##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n01_53115004-1.3410000003588d.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4778339 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.650711052522645 33.0 33.0 33.0 33.0 33.0 2 32.68628303684607 33.0 33.0 33.0 33.0 33.0 3 32.53938408304643 33.0 33.0 33.0 33.0 33.0 4 36.56677184268425 37.0 37.0 37.0 37.0 37.0 5 36.64518423661444 37.0 37.0 37.0 37.0 37.0 6 36.5251946335327 37.0 37.0 37.0 37.0 37.0 7 36.36875659094091 37.0 37.0 37.0 37.0 37.0 8 36.33073752197155 37.0 37.0 37.0 37.0 37.0 9 36.38896068278119 37.0 37.0 37.0 37.0 37.0 10-11 36.31590538050984 37.0 37.0 37.0 35.0 37.0 12-13 36.32370892898139 37.0 37.0 37.0 35.0 37.0 14-15 38.15268171638722 40.0 37.0 40.0 37.0 40.0 16-17 38.193009432775696 40.0 37.0 40.0 37.0 40.0 18-19 38.16232533941188 40.0 37.0 40.0 37.0 40.0 20-21 38.17802284433984 40.0 37.0 40.0 37.0 40.0 22-23 37.889664065274566 40.0 37.0 40.0 33.0 40.0 24-25 38.03261530837389 40.0 37.0 40.0 35.0 40.0 26-27 38.03040993952082 40.0 37.0 40.0 37.0 40.0 28-29 37.79679717156945 40.0 37.0 40.0 33.0 40.0 30-31 37.75009956388611 40.0 37.0 40.0 33.0 40.0 32-33 37.593699609843505 40.0 37.0 40.0 33.0 40.0 34-35 37.527476284123 40.0 37.0 40.0 33.0 40.0 36-37 37.581219227015914 40.0 37.0 40.0 33.0 40.0 38-39 37.385116459924674 40.0 37.0 40.0 33.0 40.0 40-41 37.09058628950353 40.0 37.0 40.0 33.0 40.0 42-43 36.88395329423049 37.0 37.0 40.0 33.0 40.0 44-45 36.80558872026451 37.0 37.0 40.0 33.0 40.0 46-47 37.02855102997087 37.0 37.0 40.0 33.0 40.0 48-49 36.728509530194486 37.0 37.0 40.0 33.0 40.0 50-51 36.69439181690541 37.0 37.0 40.0 33.0 40.0 52-53 36.61379037778609 37.0 37.0 40.0 33.0 40.0 54-55 36.333278049129625 37.0 37.0 40.0 33.0 40.0 56-57 36.24351631393252 37.0 37.0 40.0 33.0 40.0 58-59 36.124103480309785 37.0 37.0 40.0 33.0 40.0 60-61 35.825091522388846 37.0 37.0 40.0 30.0 40.0 62-63 35.61203223546927 37.0 35.0 40.0 27.0 40.0 64-65 35.23598472188767 37.0 33.0 38.5 27.0 40.0 66-67 34.39626029044821 37.0 33.0 37.0 27.0 40.0 68-69 34.205837007378506 37.0 33.0 37.0 27.0 40.0 70-71 33.66624971564387 37.0 33.0 37.0 27.0 38.5 72-73 32.38449113802935 37.0 33.0 37.0 22.0 37.0 74-75 31.407169625260995 35.0 30.0 37.0 15.0 37.0 76-77 28.90855786916751 33.0 27.0 35.0 15.0 37.0 78-79 30.692278216342544 33.0 30.0 37.0 15.0 37.0 80-81 30.877274927542814 33.0 33.0 37.0 15.0 37.0 82-83 30.75708923121612 33.0 33.0 37.0 15.0 37.0 84-85 30.570503160198555 33.0 33.0 37.0 15.0 37.0 86-87 30.41077977096225 33.0 30.0 37.0 10.5 37.0 88-89 30.30029702790028 33.0 27.0 37.0 6.0 37.0 90-91 30.17116878898714 33.0 27.0 37.0 6.0 37.0 92-93 30.015626978328662 33.0 27.0 37.0 6.0 37.0 94-95 29.89870915395496 33.0 27.0 37.0 6.0 37.0 96-97 29.762539765387093 33.0 27.0 37.0 6.0 37.0 98-99 29.600577418220013 33.0 27.0 37.0 6.0 37.0 100-101 28.172488389794026 33.0 24.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 0.16752319700375295 2306 2 0.140942547352239 2306 3 0.34312979573233093 2306 4 0.2551044999853005 2306 5 0.15344757863450553 2306 6 0.21607556762472058 2306 7 0.24350964206738013 2306 8 0.2101902575790504 2306 9 0.25822525450757183 2306 10-11 0.24213312178289215 2306 12-13 0.18677921080796267 2306 14-15 0.45009894853714627 2306 16-17 0.4969063405770129 2306 18-19 0.49011216181239803 2306 20-21 0.4490561552253709 2306 22-23 0.445821714924314 2306 24-25 0.4223562430139509 2306 26-27 0.45858262834430974 2306 28-29 0.5126907960901121 2306 30-31 0.3572418136604938 2306 32-33 0.48077292051770826 2306 34-35 0.3545306859417181 2306 36-37 0.47660157880908116 2306 38-39 0.55361387044195 2306 40-41 0.6030339622760366 2306 42-43 0.5350399781857718 2306 44-45 0.6122292337988569 2306 46-47 0.6041483054560572 2306 48-49 0.5730371793184759 2306 50-51 0.5600245341522623 2306 52-53 0.5208372619844113 2306 54-55 0.5413324317045252 2306 56-57 0.5136071405683253 2306 58-59 0.558701838156928 2306 60-61 0.5980751049469717 2306 62-63 0.5865190456244491 2306 64-65 0.3783926136727658 2306 66-67 0.2113086062573828 2306 68-69 0.4264374426839126 2306 70-71 0.43342586080698453 2306 72-73 0.018608391643326172 2306 74-75 -0.034291518215113115 2306 76-77 0.029984844221136342 2306 78-79 0.2919554463026195 2306 80-81 0.41989353638193094 2306 82-83 0.39012031837691197 2306 84-85 0.4849781300405702 2306 86-87 0.4699429634421932 2306 88-89 0.4330000823589444 2306 90-91 0.2895249363407224 2306 92-93 0.41918427665610025 2306 94-95 0.4326946138948955 2306 96-97 0.45461290342450766 2306 98-99 0.29573078551018384 2306 100-101 0.42301858015173366 2307 1 0.16638408695389018 2307 2 0.13524364037532166 2307 3 0.3486691220643152 2307 4 0.23204595865581723 2307 5 0.17239195130747476 2307 6 0.19033200709838383 2307 7 0.21472543066249017 2307 8 0.1711765548726234 2307 9 0.20369766402107814 2307 10-11 0.2465978416685104 2307 12-13 0.22419284311384047 2307 14-15 0.42181479882346906 2307 16-17 0.3742187176482048 2307 18-19 0.4335932607142894 2307 20-21 0.4698905469863277 2307 22-23 0.4431271245176518 2307 24-25 0.42883499946430703 2307 26-27 0.5502057331540442 2307 28-29 0.48459657365338416 2307 30-31 0.42769911789976334 2307 32-33 0.4721631863705369 2307 34-35 0.4244112227278194 2307 36-37 0.534296014987703 2307 38-39 0.4878005534870198 2307 40-41 0.5560493469751648 2307 42-43 0.45608148911237123 2307 44-45 0.5409742531408668 2307 46-47 0.4426528288733138 2307 48-49 0.45112455221644154 2307 50-51 0.2798661964037734 2307 52-53 0.35833109286340203 2307 54-55 0.5038868651251889 2307 56-57 0.45516297474235756 2307 58-59 0.568997965037596 2307 60-61 0.4717454779247703 2307 62-63 0.39797991162388513 2307 64-65 0.39634879295467584 2307 66-67 0.2175079378149789 2307 68-69 0.30637382815962155 2307 70-71 0.20955344911613594 2307 72-73 -0.3252307430830257 2307 74-75 -0.24372369598740562 2307 76-77 -0.42880814910895637 2307 78-79 0.07149596472651965 2307 80-81 0.09257920877983494 2307 82-83 0.06317223697354635 2307 84-85 0.09564440418179032 2307 86-87 0.09299084489346399 2307 88-89 0.19967340199924877 2307 90-91 0.05472170408420496 2307 92-93 0.07626161928908104 2307 94-95 0.04740433860694537 2307 96-97 0.10545530597054054 2307 98-99 0.07663177656133158 2307 100-101 0.0794251215973425 2201 1 0.16938376696970892 2201 2 0.1198139311489328 2201 3 0.30660648069791563 2201 4 0.19843131162033956 2201 5 0.14835923395513362 2201 6 0.14074733420324037 2201 7 0.18846973309010906 2201 8 0.15172670106282027 2201 9 0.1482588599653809 2201 10-11 0.15240996632226 2201 12-13 0.20304650665803337 2201 14-15 0.26484350401366896 2201 16-17 0.3260628582297542 2201 18-19 0.31415388707302583 2201 20-21 0.30070423379460465 2201 22-23 0.3167090401242305 2201 24-25 0.24654023566894523 2201 26-27 0.36232236155078823 2201 28-29 0.40934857733113716 2201 30-31 0.4367609445424989 2201 32-33 0.39439278257247423 2201 34-35 0.34199092007497 2201 36-37 0.33045622200236835 2201 38-39 0.2889078549629289 2201 40-41 0.3316167953141971 2201 42-43 0.24813168474218372 2201 44-45 0.3623171635686404 2201 46-47 0.3818238621338139 2201 48-49 0.35009924668648296 2201 50-51 0.3472738078386044 2201 52-53 0.36728215245049256 2201 54-55 0.45739431402729025 2201 56-57 0.5086726896268416 2201 58-59 0.5477853228472043 2201 60-61 0.5224933551112727 2201 62-63 0.44738974337812465 2201 64-65 0.45479384322515415 2201 66-67 0.21025207338390572 2201 68-69 0.3380239371327747 2201 70-71 0.16427800522392744 2201 72-73 -0.17623647876510518 2201 74-75 -0.2382329374189034 2201 76-77 -0.2953683947139787 2201 78-79 0.07645335910770612 2201 80-81 0.15656746137781496 2201 82-83 0.16906682965291608 2201 84-85 0.1685620436186781 2201 86-87 0.23770630788859748 2201 88-89 0.15897395503628076 2201 90-91 0.1668489070654111 2201 92-93 0.23111636638484612 2201 94-95 0.26594014318862236 2201 96-97 0.1642945856765401 2201 98-99 0.1320762884893263 2201 100-101 0.12249672595406835 1101 1 0.1769991279820431 1101 2 0.12558943940005918 1101 3 0.3092107854460622 1101 4 0.21931181901922514 1101 5 0.14725009108882148 1101 6 0.1372278838627068 1101 7 0.11823221009787943 1101 8 0.1052722165767932 1101 9 0.10917927469757416 1101 10-11 0.10456774940201541 1101 12-13 0.12051492994696389 1101 14-15 0.2002312697948696 1101 16-17 0.21154591183692872 1101 18-19 0.2253088646598158 1101 20-21 0.2627608956965517 1101 22-23 0.30883670186758394 1101 24-25 0.27609842683045827 1101 26-27 0.22482373158245395 1101 28-29 0.2838980077970277 1101 30-31 0.24046071357116716 1101 32-33 0.3120223697881599 1101 34-35 0.34099754340267197 1101 36-37 0.371080752566499 1101 38-39 0.4020657931614835 1101 40-41 0.3331573549287796 1101 42-43 0.35910223453361567 1101 44-45 0.3143156792326707 1101 46-47 0.4021312676063715 1101 48-49 0.3806100395331953 1101 50-51 0.3100215899677323 1101 52-53 0.20249624523429333 1101 54-55 0.3228588640085306 1101 56-57 0.4241481563996956 1101 58-59 0.42643599792216236 1101 60-61 0.39990145986695325 1101 62-63 0.37655960020023116 1101 64-65 0.30234612311983966 1101 66-67 0.020519894720230525 1101 68-69 0.049032994000207 1101 70-71 0.089446342308527 1101 72-73 -0.15716547995097585 1101 74-75 -0.218776709802178 1101 76-77 -0.23525966299545686 1101 78-79 0.23597889788080906 1101 80-81 0.25996263493109595 1101 82-83 0.3400677615510226 1101 84-85 0.2024779034845281 1101 86-87 0.2182820048741796 1101 88-89 0.2783206038262236 1101 90-91 0.19174100941663497 1101 92-93 0.09224920615645615 1101 94-95 0.028268314350640367 1101 96-97 0.19700995406155997 1101 98-99 0.2469768437482429 1101 100-101 0.24231129932956819 2304 1 0.12293709799268981 2304 2 0.07446776136230682 2304 3 0.27164046983923384 2304 4 0.18719025167408176 2304 5 0.11317097513683194 2304 6 0.11629277305771524 2304 7 0.09068791644325103 2304 8 0.15981129919659764 2304 9 0.09065840786841761 2304 10-11 0.09049133926935582 2304 12-13 0.080749140240016 2304 14-15 0.18184166489114517 2304 16-17 0.19693484085129853 2304 18-19 0.2576443953050571 2304 20-21 0.24491916972875316 2304 22-23 0.36924751140377765 2304 24-25 0.28609150960266305 2304 26-27 0.2737228631897395 2304 28-29 0.24841664409235165 2304 30-31 0.26943846237755054 2304 32-33 0.3000640337511271 2304 34-35 0.41524948826333485 2304 36-37 0.32885643477155213 2304 38-39 0.3062452237603992 2304 40-41 0.33663491239103394 2304 42-43 0.3376955845231464 2304 44-45 0.4783546334179505 2304 46-47 0.3275351224517422 2304 48-49 0.2329711920538955 2304 50-51 0.33576599776439764 2304 52-53 0.2921276416731615 2304 54-55 0.3934069182431017 2304 56-57 0.488510465891423 2304 58-59 0.29546858127418574 2304 60-61 0.4531489873432477 2304 62-63 0.34896116188492243 2304 64-65 0.2384930632467288 2304 66-67 0.19997113363631058 2304 68-69 0.27618451267311883 2304 70-71 0.12508702985807219 2304 72-73 -0.17281540743530144 2304 74-75 -0.09178062023418576 2304 76-77 -0.13677847727545256 2304 78-79 0.24857576628369316 2304 80-81 0.20783549902672505 2304 82-83 0.18093501340413454 2304 84-85 0.19232623905927682 2304 86-87 0.29676688176152055 2304 88-89 0.43016509894705734 2304 90-91 0.39448279324711777 2304 92-93 0.2949106565576116 2304 94-95 0.37696204967203073 2304 96-97 0.3051846951391042 2304 98-99 0.2650347595916074 2304 100-101 0.12415998041228349 2202 1 0.12086284217902943 2202 2 0.08420369437133246 2202 3 0.31237072714154124 2202 4 0.21216253881451763 2202 5 0.1215326381705637 2202 6 0.11191590181321232 2202 7 0.1381485497897188 2202 8 0.11887144451915788 2202 9 0.21208930897264366 2202 10-11 0.1624915073584816 2202 12-13 0.11972345730082878 2202 14-15 0.06188809758494074 2202 16-17 0.10119838044335694 2202 18-19 0.04826715524114178 2202 20-21 0.11821421523747233 2202 22-23 0.12010819713030685 2202 24-25 0.18198227017511925 2202 26-27 0.12980689284642466 2202 28-29 0.18254735986160853 2202 30-31 0.1295606791081667 2202 32-33 0.2237736091513085 2202 34-35 0.1988962281438731 2202 36-37 0.14865615169301094 2202 38-39 0.13204551691400468 2202 40-41 0.17343816817976432 2202 42-43 0.15059914942624886 2202 44-45 0.2506155838206112 2202 46-47 0.24553545040545544 2202 48-49 0.1607014131167972 2202 50-51 0.23790246454636588 2202 52-53 0.20908531585423162 2202 54-55 0.31944352569048107 2202 56-57 0.33429241831088774 2202 58-59 0.39757083909155 2202 60-61 0.26930009073014105 2202 62-63 0.18834107749595574 2202 64-65 0.1283899942836655 2202 66-67 0.17984040450217265 2202 68-69 0.0260771438154066 2202 70-71 0.27969201655358944 2202 72-73 -0.05088627884892105 2202 74-75 -0.1383140647016745 2202 76-77 -0.25529339184428324 2202 78-79 0.07163657933645595 2202 80-81 0.12030796929178322 2202 82-83 0.05147001745086399 2202 84-85 0.13367386408070914 2202 86-87 0.17559139131522983 2202 88-89 0.1655299718662775 2202 90-91 0.018915203298231376 2202 92-93 0.13116710864848358 2202 94-95 0.09110926850377155 2202 96-97 0.2004156930359322 2202 98-99 0.027026980714651927 2202 100-101 -0.018382876532303527 1102 1 0.09998565680091076 1102 2 0.06011084118725307 1102 3 0.27820058883774834 1102 4 0.18560232324021797 1102 5 0.09452984365284323 1102 6 0.013327332939404357 1102 7 0.13994005091034722 1102 8 0.1438369148387295 1102 9 0.06565168593935056 1102 10-11 0.08344629928915737 1102 12-13 0.09208151060608571 1102 14-15 0.15674366222070546 1102 16-17 0.03482164458421266 1102 18-19 0.03868182066363346 1102 20-21 0.03197329449794495 1102 22-23 0.06139958282732749 1102 24-25 0.0864674029317527 1102 26-27 0.03727410669569764 1102 28-29 0.10069722403188308 1102 30-31 0.06474910207094808 1102 32-33 0.07775774497908827 1102 34-35 0.031308749706653316 1102 36-37 0.017904451751547867 1102 38-39 0.16939820569979958 1102 40-41 0.13762220845432438 1102 42-43 -0.0036067994191810726 1102 44-45 -0.02835367356236418 1102 46-47 0.13809371984994812 1102 48-49 0.13468945971092694 1102 50-51 0.17162641996264938 1102 52-53 0.06151385061719594 1102 54-55 0.15129920136668318 1102 56-57 0.09817466479533721 1102 58-59 0.1034329341713871 1102 60-61 0.04356644460043668 1102 62-63 0.049139955013487224 1102 64-65 -0.0946779507766422 1102 66-67 -0.18661069923005158 1102 68-69 -0.13693569986205745 1102 70-71 -0.2876613759753397 1102 72-73 -0.3594244972148175 1102 74-75 -0.267274020012767 1102 76-77 -0.3772498245435507 1102 78-79 -0.36221180955443444 1102 80-81 -0.330003869274897 1102 82-83 -0.26097708005919884 1102 84-85 -0.32328786008510235 1102 86-87 -0.2660776171033916 1102 88-89 -0.20805036637848673 1102 90-91 -0.334918504390739 1102 92-93 -0.25733203614532485 1102 94-95 -0.36452337952823655 1102 96-97 -0.41698467252087923 1102 98-99 -0.502767531160341 1102 100-101 -0.5982860302755846 2305 1 0.07499716918371746 2305 2 0.03147793126572651 2305 3 0.2547309826042792 2305 4 0.09488953459680971 2305 5 0.05590569174964344 2305 6 0.03772809727554716 2305 7 0.03709154310882923 2305 8 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-0.09977602420613607 1213 96-97 -0.10043341027784791 1213 98-99 -0.12429052969503473 1213 100-101 0.11022201911352525 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 10.0 8 91.0 9 268.0 10 198.0 11 104.0 12 95.0 13 168.0 14 397.0 15 836.0 16 1629.0 17 2752.0 18 4360.0 19 6318.0 20 8966.0 21 12445.0 22 17157.0 23 22991.0 24 31509.0 25 42902.0 26 60498.0 27 90424.0 28 156945.0 29 259999.0 30 227139.0 31 117616.0 32 127095.0 33 180213.0 34 276833.0 35 482639.0 36 941892.0 37 1385588.0 38 318200.0 39 62.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.52391886784424 22.939649789044573 19.808365645174376 13.728065697936815 2 26.513857639652606 32.5861141287799 28.286314554074128 12.61371367749337 3 32.70949020349753 17.439117952727496 34.41374385822016 15.43764798555481 4 25.87127088233675 17.11844528178347 28.617757759974616 28.392526075905174 5 40.89881442065956 15.982352863620603 27.404439073912506 15.71439364180733 6 35.832995524176916 17.02256788394461 31.407022398368973 15.737414193509503 7 28.478871005175648 30.13270929500816 24.350051346294183 17.038368353522007 8 25.064379065612545 36.035743801350215 22.248254048111697 16.651623084925536 9 38.24799789215457 21.7216484640374 22.583098436506912 17.447255207301115 10-11 27.244504228200945 31.733319102427473 25.660342807872834 15.361833861498756 12-13 20.57093910058267 30.362125910724608 25.624641873387777 23.442293115304945 14-15 20.16282226941203 30.849380925045295 25.954458233289852 23.033338572252827 16-17 26.050757386614887 24.774351505826605 26.512193881597767 22.662697225960738 18-19 18.80196235553819 24.88744519800709 33.18433455642222 23.126257890032498 20-21 25.294092779938804 25.065781226488955 32.845315077059205 16.79481091651304 22-23 18.563396192693737 38.00181401947413 26.419086213849624 17.015703573982506 24-25 18.747497822988283 24.472949700722364 33.04350109944062 23.73605137684873 26-27 18.67662982400994 24.662884877545288 26.313919191149726 30.346566107295047 28-29 24.61333014951761 31.671910300944877 26.0467901623024 17.66796938723511 30-31 17.55204057309454 26.08176188420286 32.284743715337065 24.081453827365536 32-33 17.364559107254635 32.43089282698444 26.38091395357257 23.82363411218836 34-35 17.755029519672004 32.35145099583768 33.250947661938596 16.642571822551727 36-37 17.691450771834532 26.44116902884316 33.01705567953061 22.850324519791698 38-39 24.918674141558057 32.47002914002195 25.67442282916882 16.936873889251174 40-41 18.224493870442384 38.49838918348324 25.599300340161253 17.677816605913122 42-43 18.274241598223636 32.01567292167411 32.49488050679059 17.215204973311664 44-45 17.700685837209956 25.599381623731603 32.951965026205336 23.747967512853105 46-47 24.457738887155315 25.521426850811824 26.413930639382677 23.60690362265018 48-49 18.358973189124796 32.40021866098193 32.02930283185644 17.21150531803683 50-51 24.897447158641956 25.725875085663446 31.533367593480023 17.843310162214575 52-53 18.187712424866476 25.60346451353659 25.378744119075126 30.83007894252181 54-55 24.926308664769934 25.709025336340986 31.76421995806647 17.600446040822614 56-57 18.335293066229205 25.70679102912558 31.787471048766164 24.170444855879055 58-59 18.2847185519699 25.743894589771898 32.16838216440521 23.803004693852987 60-61 24.451711599092487 26.019901762607656 31.944575685057693 17.58381095324217 62-63 17.845139337176683 26.111767970086714 32.04281560161161 24.000277091124993 64-65 24.66534396669969 26.035635039877214 31.819539883338827 17.479481110084265 66-67 18.739690023265577 38.296098188290934 25.161947944933356 17.802263843510136 68-69 18.781897974627103 38.43413796315582 25.121060800959572 17.662903261257508 70-71 18.27517023047223 39.20107621181897 25.218256204854526 17.305497352854275 72-73 18.120796097007133 39.08979567144912 25.05621950001852 17.733188731525225 74-75 18.85453934785983 37.835670155511245 24.93862224323416 18.37116825339476 76-77 19.22789941462272 36.11053225907233 25.56615305424722 19.095415272057725 78-79 18.92610501718639 35.157446660658096 26.418918381503083 19.497529940652434 80-81 18.451432446177318 34.97826582869246 26.353043725762266 20.21725799936796 82-83 17.994208176046335 34.402199532782404 26.62187028302504 20.981722008146225 84-85 18.328182724594587 33.78597368480535 26.8696228356351 21.01622075496497 86-87 18.447488345540275 33.460546468446225 27.546459550691733 20.545505635321764 88-89 18.76006194331937 33.772002578285495 27.18428221237324 20.2836532660219 90-91 19.123994265294797 33.00934495708705 26.826513863999402 21.040146913618752 92-93 18.67119231602513 33.60357446755066 26.52555934786272 21.199673868561494 94-95 18.433295095830996 33.7443376399367 26.374791717254602 21.447575546977703 96-97 18.948201673632372 34.0302353240029 26.117371134274194 20.90419186809053 98-99 19.445307123249673 32.955987680004604 26.027680737315613 21.571024459430106 100-101 19.454155782078036 33.222466565442915 25.50888798901366 21.814489663465388 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 95.0 1 68.0 2 120.5 3 331.0 4 344.0 5 201.5 6 178.0 7 175.5 8 186.0 9 195.5 10 185.5 11 182.5 12 170.5 13 173.5 14 185.5 15 190.5 16 205.5 17 240.5 18 309.5 19 430.5 20 604.0 21 823.0 22 1222.0 23 1827.0 24 2656.5 25 3892.0 26 5651.5 27 7794.0 28 10425.0 29 13739.5 30 17771.5 31 22780.5 32 31078.5 33 48909.0 34 79500.5 35 131282.5 36 200241.0 37 281562.5 38 365829.0 39 407644.0 40 408068.0 41 374178.5 42 329431.0 43 315873.5 44 294948.0 45 263253.0 46 249673.5 47 220385.5 48 167170.0 49 122046.0 50 92303.0 51 74110.5 52 60289.5 53 46759.5 54 35474.5 55 25510.5 56 17310.5 57 12016.5 58 8704.5 59 6329.0 60 4617.0 61 3438.5 62 2383.0 63 1568.5 64 1021.5 65 660.5 66 439.5 67 298.0 68 208.0 69 143.0 70 101.5 71 74.0 72 51.0 73 36.5 74 29.0 75 20.0 76 15.5 77 11.5 78 11.5 79 8.5 80 3.5 81 3.0 82 2.5 83 0.5 84 0.0 85 0.5 86 0.5 87 0.5 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16532941677013707 2 0.0 3 2.0927774274700895E-5 4 0.021681174148590127 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.360305327855558E-4 12-13 2.0927774274700895E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 2.0927774274700895E-5 28-29 1.1510275851085493E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0010045331651856429 38-39 6.487610025157277E-4 40-41 3.557721626699152E-4 42-43 7.115443253398304E-4 44-45 3.8716382408196656E-4 46-47 0.0031810216897545365 48-49 0.003474010529600349 50-51 0.004028596547879923 52-53 0.0037460715951714604 54-55 0.006299260056684969 56-57 0.009982548329032328 58-59 0.01703520825960653 60-61 0.004593646453296846 62-63 0.0026682912200243643 64-65 0.010160434410367285 66-67 0.023219365557780643 68-69 0.024987762483992867 70-71 0.02537492630807484 72-73 0.022643851765226368 74-75 0.02428668204579039 76-77 0.024349465368614494 78-79 0.015088925252059347 80-81 0.0031182383669304337 82-83 0.001077780375147096 84-85 0.005504004634246336 86-87 0.013843722682714641 88-89 0.015601655721789518 90-91 0.023229829444917995 92-93 0.023522818284763804 94-95 0.024673845869872358 96-97 0.026044615084865264 98-99 0.03246944178719844 100-101 0.023721632140373466 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4778339.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.13501545835088 #Duplication Level Percentage of deduplicated Percentage of total 1 88.00010722854478 30.03885020582911 2 5.4176993020153486 3.698664988459814 3 1.8570780517910273 1.9017416401575251 4 0.8312808728178094 1.1350314177546934 5 0.4778635690977022 0.8155940159066396 6 0.5420204537693485 1.1101125940895442 7 0.42668162347170224 1.0195348669100563 8 0.24918275697902995 0.6804685809146943 9 0.22774266567304907 0.699659947194801 >10 1.5061063519740192 10.215497216153011 >50 0.21113088919234246 5.098556634783731 >100 0.2015528221727444 14.125289526967133 >500 0.03472442007946923 8.320981731377628 >1k 0.016461155222239638 8.906561989298469 >5k 3.0653101160409813E-4 0.7384887103556225 >10k+ 6.130618773167038E-5 11.494965933847547 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT 549292 11.495458986899004 TruSeq Adapter, Index 13 (97% over 38bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 8671 0.18146473073593147 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 6947 0.14538524788634713 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATATCGTAT 6779 0.14186938180819736 TruSeq Adapter, Index 13 (97% over 38bp) TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 6731 0.14086484864301174 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 6161 0.12893601730643223 No Hit GTGTTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAAT 4838 0.10124857194100292 No Hit ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG 4827 0.10101836642398122 TruSeq Adapter, Index 13 (97% over 37bp) TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 4797 0.10039053319574019 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0063201878309596705 0.0 0.0 2 0.006864309962101894 0.0 0.0 3 0.007429359867518818 0.0 0.0 4 0.007638637610265827 0.0 0.0 5 0.00839203748415506 0.0 0.0 6 0.009124509583769591 0.0 0.0 7 0.010233681620328738 0.0 0.0 8 0.011280070334063783 0.0 0.0 9 0.012263675724974724 0.0 0.0 10-11 0.017976958101968067 0.0 0.0 12-13 0.02244503790961671 0.0 0.0 14-15 0.027488631509819627 0.0 0.0 16-17 0.03145444473487544 0.0 0.0 18-19 0.03590159676824939 0.0 0.0 20-21 0.0410079736912764 0.0 0.0 22-23 0.04657476164834684 0.0 0.0 24-25 0.05250778565522454 0.0 0.0 26-27 0.05891168458328302 0.0 0.0 28-29 0.06613176670805482 0.0 0.0 30-31 0.10272398002736935 0.0 0.0 32-33 0.11151364522274372 0.0 0.0 34-35 0.11879651067033964 0.0 0.0 36-37 0.12740828978437904 0.0 0.0 38-39 0.13606192444696785 0.0 0.0 40-41 0.14555267008054473 0.0 0.0 42-43 0.15605841276644458 0.0 2.0927774274700895E-5 44-45 0.16752683306898067 0.0 2.0927774274700895E-5 46-47 0.17908942835575292 0.0 2.0927774274700895E-5 48-49 0.19111243467656858 0.0 2.0927774274700895E-5 50-51 0.20469456018084947 0.0 2.0927774274700895E-5 52-53 0.21896730223619548 0.0 2.0927774274700895E-5 54-55 0.23527003839618746 0.0 2.0927774274700895E-5 56-57 0.25053684972958173 0.0 2.0927774274700895E-5 58-59 0.2678231912804847 0.0 2.0927774274700895E-5 60-61 0.2871395269360336 0.0 2.0927774274700895E-5 62-63 0.30822425951779475 0.0 2.0927774274700895E-5 64-65 0.32966476426222585 0.0 2.0927774274700895E-5 66-67 0.353198046434127 0.0 2.0927774274700895E-5 68-69 0.37905431155051994 0.0 3.139166141205134E-5 70-71 0.4065429430603396 0.0 4.185554854940179E-5 72-73 0.436354557514651 0.0 5.231943568675223E-5 74-75 0.4696925019342495 0.0 1.0463887137350448E-4 76-77 0.5079484733084028 0.0 1.2556664564820536E-4 78-79 0.5491657247424262 0.0 1.2556664564820536E-4 80-81 0.5942441505301319 0.0 1.2556664564820536E-4 82-83 0.645872969665819 0.0 1.2556664564820536E-4 84-85 0.7031941434042248 0.0 1.2556664564820536E-4 86-87 0.7666890105536672 0.0 1.2556664564820536E-4 88-89 0.8372470015208213 0.0 1.2556664564820536E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCGGA 67385 0.0 87.171196 1 TCGGAAG 67450 0.0 86.69354 3 CGGAAGA 68055 0.0 86.41842 4 ATCGGAA 67950 0.0 86.11853 2 GAAGAGC 68720 0.0 85.48221 6 GGAAGAG 70725 0.0 83.17301 5 AGAGCAC 86450 0.0 67.98365 8 GAGCACA 86515 0.0 67.94904 9 AAGAGCA 87275 0.0 67.248505 7 TCGGACG 245 0.0 56.226696 3 GAGCGGA 430 0.0 55.319256 1 ACGGCGA 115 0.0 53.779938 1 TCGCCGG 85 1.2914825E-10 50.29609 2 GCGGAAG 485 0.0 48.97099 3 ATCGGAC 290 0.0 47.501865 2 GAACGGA 590 0.0 46.768215 1 GCCGTCT 63790 0.0 46.09901 50-51 ATGCCGT 63715 0.0 46.061405 48-49 CGTCTTC 63830 0.0 46.046963 52-53 TCGTATG 64165 0.0 46.003162 44-45 >>END_MODULE