FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n01_53115004-1.3410000003588d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n01_53115004-1.3410000003588d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4778339
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT54929211.495458986899004TruSeq Adapter, Index 13 (97% over 38bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC86710.18146473073593147No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG69470.14538524788634713No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATATCGTAT67790.14186938180819736TruSeq Adapter, Index 13 (97% over 38bp)
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA67310.14086484864301174No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC61610.12893601730643223No Hit
GTGTTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAAT48380.10124857194100292No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG48270.10101836642398122TruSeq Adapter, Index 13 (97% over 37bp)
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG47970.10039053319574019No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA673850.087.1711961
TCGGAAG674500.086.693543
CGGAAGA680550.086.418424
ATCGGAA679500.086.118532
GAAGAGC687200.085.482216
GGAAGAG707250.083.173015
AGAGCAC864500.067.983658
GAGCACA865150.067.949049
AAGAGCA872750.067.2485057
TCGGACG2450.056.2266963
GAGCGGA4300.055.3192561
ACGGCGA1150.053.7799381
TCGCCGG851.2914825E-1050.296092
GCGGAAG4850.048.970993
ATCGGAC2900.047.5018652
GAACGGA5900.046.7682151
GCCGTCT637900.046.0990150-51
ATGCCGT637150.046.06140548-49
CGTCTTC638300.046.04696352-53
TCGTATG641650.046.00316244-45
Produced by FastQC (version 0.11.2)