##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n01_52370180-2.341000000358c0.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5323333 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.68493554695902 33.0 33.0 33.0 33.0 33.0 2 32.73019704008748 33.0 33.0 33.0 33.0 33.0 3 32.57168018607891 33.0 33.0 33.0 33.0 33.0 4 36.61434011360927 37.0 37.0 37.0 37.0 37.0 5 36.69549415751372 37.0 37.0 37.0 37.0 37.0 6 36.58799590406988 37.0 37.0 37.0 37.0 37.0 7 36.469129584792086 37.0 37.0 37.0 37.0 37.0 8 36.41924467246366 37.0 37.0 37.0 37.0 37.0 9 36.50185288051677 37.0 37.0 37.0 37.0 37.0 10-11 36.42511355197956 37.0 37.0 37.0 37.0 37.0 12-13 36.410428767090096 37.0 37.0 37.0 37.0 37.0 14-15 38.29880020656232 40.0 37.0 40.0 37.0 40.0 16-17 38.343364016491165 40.0 37.0 40.0 37.0 40.0 18-19 38.318207033074955 40.0 37.0 40.0 37.0 40.0 20-21 38.34036617660402 40.0 37.0 40.0 37.0 40.0 22-23 38.14566391018559 40.0 37.0 40.0 37.0 40.0 24-25 38.190891026355104 40.0 37.0 40.0 37.0 40.0 26-27 38.16626838862044 40.0 37.0 40.0 37.0 40.0 28-29 37.97536421260891 40.0 37.0 40.0 35.0 40.0 30-31 38.00394940538193 40.0 37.0 40.0 37.0 40.0 32-33 37.88831855906817 40.0 37.0 40.0 33.0 40.0 34-35 37.71244941843766 40.0 37.0 40.0 33.0 40.0 36-37 37.37588800099486 40.0 37.0 40.0 33.0 40.0 38-39 37.616026932750586 40.0 37.0 40.0 33.0 40.0 40-41 37.40787237995444 40.0 37.0 40.0 33.0 40.0 42-43 37.20711798416518 40.0 37.0 40.0 33.0 40.0 44-45 37.28812841503622 40.0 37.0 40.0 33.0 40.0 46-47 37.33146235638462 40.0 37.0 40.0 33.0 40.0 48-49 37.044578932033744 38.5 37.0 40.0 33.0 40.0 50-51 36.9808278948546 37.0 37.0 40.0 33.0 40.0 52-53 36.933148649539675 37.0 37.0 40.0 33.0 40.0 54-55 36.677948570942306 37.0 37.0 40.0 33.0 40.0 56-57 36.59190398196017 37.0 37.0 40.0 33.0 40.0 58-59 36.329102086981976 37.0 37.0 40.0 33.0 40.0 60-61 36.051451787817896 37.0 37.0 40.0 33.0 40.0 62-63 35.86615650382946 37.0 37.0 40.0 33.0 40.0 64-65 35.59579590455829 37.0 35.0 40.0 30.0 40.0 66-67 34.990673230474215 37.0 33.0 37.0 27.0 40.0 68-69 34.839966520974734 37.0 33.0 37.0 27.0 40.0 70-71 34.40245537898906 37.0 33.0 37.0 27.0 40.0 72-73 33.525525455574545 37.0 33.0 37.0 24.5 40.0 74-75 32.682792998296364 37.0 33.0 37.0 22.0 38.5 76-77 30.212170645721393 33.0 30.0 35.0 18.5 37.0 78-79 32.144226840590285 35.0 33.0 37.0 18.5 37.0 80-81 32.344114016538136 37.0 33.0 37.0 15.0 37.0 82-83 32.22331676414006 37.0 33.0 37.0 15.0 37.0 84-85 32.04026349657254 37.0 33.0 37.0 15.0 37.0 86-87 31.877882991727176 37.0 33.0 37.0 15.0 37.0 88-89 31.757198826374378 37.0 33.0 37.0 15.0 37.0 90-91 31.613314252555682 37.0 33.0 37.0 15.0 37.0 92-93 31.45522776801677 33.0 33.0 37.0 15.0 37.0 94-95 31.34541066658802 33.0 33.0 37.0 15.0 37.0 96-97 31.221075405201965 33.0 33.0 37.0 15.0 37.0 98-99 31.06385830080515 33.0 33.0 37.0 15.0 37.0 100-101 29.636099601509052 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 0.14272866188389344 2306 2 0.11233785997393397 2306 3 0.31445079178696744 2306 4 0.2047474324623053 2306 5 0.13388554863581703 2306 6 0.17779526279574043 2306 7 0.20452899110013334 2306 8 0.1803288318962899 2306 9 0.1423979338035224 2306 10-11 0.2178009958741498 2306 12-13 0.1945128696902998 2306 14-15 0.36231951141638064 2306 16-17 0.394860384704522 2306 18-19 0.3936229120815753 2306 20-21 0.34690968119403465 2306 22-23 0.46707853440877045 2306 24-25 0.40735378497731745 2306 26-27 0.4410698465910272 2306 28-29 0.4794838422875145 2306 30-31 0.3682920112272896 2306 32-33 0.39853377310070925 2306 34-35 0.45154191388062515 2306 36-37 0.3720567891440467 2306 38-39 0.45305139038358533 2306 40-41 0.5411717707625101 2306 42-43 0.4789367138245453 2306 44-45 0.515399411619633 2306 46-47 0.5054673371670972 2306 48-49 0.5425680850167183 2306 50-51 0.49020749421515575 2306 52-53 0.44969179633405787 2306 54-55 0.5529796590631761 2306 56-57 0.5934684452431327 2306 58-59 0.5609876717036641 2306 60-61 0.48276316589491586 2306 62-63 0.5892046481024238 2306 64-65 0.5429275664393671 2306 66-67 0.2711029396848801 2306 68-69 0.4431014888101714 2306 70-71 0.4544457826102004 2306 72-73 0.1412980709154752 2306 74-75 0.13422867386150727 2306 76-77 0.0646694855697092 2306 78-79 0.2631290398343751 2306 80-81 0.3987338027451415 2306 82-83 0.41590258174744577 2306 84-85 0.38083861440121325 2306 86-87 0.3679139972538472 2306 88-89 0.5225768673695796 2306 90-91 0.45762542038922405 2306 92-93 0.5239474557414425 2306 94-95 0.454178496778308 2306 96-97 0.561185716173771 2306 98-99 0.3716224105801835 2306 100-101 0.4489390916401099 2307 1 0.13176525767078573 2307 2 0.11767790261595223 2307 3 0.32709209673199524 2307 4 0.23108541277986205 2307 5 0.12616788167434123 2307 6 0.11540760394282046 2307 7 0.12637835704126843 2307 8 0.18587316083627314 2307 9 0.1743369661806753 2307 10-11 0.18895559853403654 2307 12-13 0.2202657890026103 2307 14-15 0.3440957852271822 2307 16-17 0.3688419169383792 2307 18-19 0.3500083511381078 2307 20-21 0.3513325715122946 2307 22-23 0.3764959844102407 2307 24-25 0.3957738728411897 2307 26-27 0.4055640513987484 2307 28-29 0.3968077890916675 2307 30-31 0.3519383679713286 2307 32-33 0.3813013071603848 2307 34-35 0.3062386415855656 2307 36-37 0.4274323786699128 2307 38-39 0.4038941025129219 2307 40-41 0.3954474657720297 2307 42-43 0.3945157126947123 2307 44-45 0.34908372420009925 2307 46-47 0.3610030745718049 2307 48-49 0.3496683006476431 2307 50-51 0.3079396160787269 2307 52-53 0.2714023983004523 2307 54-55 0.3870177198017686 2307 56-57 0.31346271597071507 2307 58-59 0.32255743775876766 2307 60-61 0.2792828583701592 2307 62-63 0.32137721241107187 2307 64-65 0.2583797748970582 2307 66-67 0.13812415947166556 2307 68-69 0.23266007969870373 2307 70-71 0.22508552209206556 2307 72-73 -0.08609379055100419 2307 74-75 -0.22929677601037923 2307 76-77 -0.3676642530926415 2307 78-79 0.18575642032154605 2307 80-81 0.22784469513865702 2307 82-83 0.2582182425854427 2307 84-85 0.30652388130663155 2307 86-87 0.28560993736182283 2307 88-89 0.4166028607984771 2307 90-91 0.25195700072500316 2307 92-93 0.17883714292055686 2307 94-95 0.28048518444597903 2307 96-97 0.23585213260999538 2307 98-99 0.0666782371659771 2307 100-101 0.03895108774923628 2201 1 0.16021123188609465 2201 2 0.1110784956216122 2201 3 0.3097932985661771 2201 4 0.2375739234938905 2201 5 0.12893994816125343 2201 6 0.1840269758998403 2201 7 0.16492247616725564 2201 8 0.15854579751395192 2201 9 0.11761198645869086 2201 10-11 0.13291114967038453 2201 12-13 0.18348437590023536 2201 14-15 0.3388740687162155 2201 16-17 0.31084543247532537 2201 18-19 0.3095762143268317 2201 20-21 0.3408432816866167 2201 22-23 0.33986485449632653 2201 24-25 0.26759059908765437 2201 26-27 0.3092937078336675 2201 28-29 0.3669638634042087 2201 30-31 0.3873496388018367 2201 32-33 0.3994008967873981 2201 34-35 0.38579383966992964 2201 36-37 0.3320643017703233 2201 38-39 0.3848520647636917 2201 40-41 0.4091085935270442 2201 42-43 0.4258447631841875 2201 44-45 0.4967925185134874 2201 46-47 0.44817997990497105 2201 48-49 0.44839300477477195 2201 50-51 0.35662682886359676 2201 52-53 0.40924375055857354 2201 54-55 0.4530651380069628 2201 56-57 0.5438439494597134 2201 58-59 0.47721660252475573 2201 60-61 0.3922428651079386 2201 62-63 0.4706260688854087 2201 64-65 0.19824975723765448 2201 66-67 0.18217245712862962 2201 68-69 0.3609984606856642 2201 70-71 0.26154792393978 2201 72-73 -6.668217107019814E-4 2201 74-75 0.10984663705703213 2201 76-77 -0.0330334786824622 2201 78-79 0.2236583171814246 2201 80-81 0.3364147074677817 2201 82-83 0.34564548747462 2201 84-85 0.42220612498469734 2201 86-87 0.4008688569074188 2201 88-89 0.4332453050841707 2201 90-91 0.38280127442473955 2201 92-93 0.2703032484997152 2201 94-95 0.33716703153078953 2201 96-97 0.3006143117343889 2201 98-99 0.28911160121428736 2201 100-101 0.12111223491393375 1101 1 0.14920347728798333 1101 2 0.10654496249487266 1101 3 0.3190405749988514 1101 4 0.1692186614996416 1101 5 0.1469341702087945 1101 6 0.14085437860779138 1101 7 0.12005676108235974 1101 8 0.12911683448134426 1101 9 0.14963405905474048 1101 10-11 0.15894926992199743 1101 12-13 0.1274188338340565 1101 14-15 0.20283002583561682 1101 16-17 0.15712683805752903 1101 18-19 0.18027096243134366 1101 20-21 0.253845268711693 1101 22-23 0.20849912717852703 1101 24-25 0.23251594187092195 1101 26-27 0.16259465205451562 1101 28-29 0.18700355836339355 1101 30-31 0.1897928662406443 1101 32-33 0.255005385699576 1101 34-35 0.25805585499696093 1101 36-37 0.27603153963388394 1101 38-39 0.21397955835518445 1101 40-41 0.324209952353101 1101 42-43 0.3214223376028329 1101 44-45 0.26279579671828657 1101 46-47 0.24878771662505272 1101 48-49 0.3211800285403825 1101 50-51 0.26977399549674885 1101 52-53 0.2285537081480129 1101 54-55 0.3742067946463621 1101 56-57 0.4037398554424243 1101 58-59 0.39288128364912467 1101 60-61 0.3676140964883743 1101 62-63 0.29907927031950976 1101 64-65 0.28385597358328596 1101 66-67 0.1451465203137161 1101 68-69 0.29914281269317655 1101 70-71 0.18565213954465065 1101 72-73 0.1455023271751017 1101 74-75 0.12410423837322782 1101 76-77 -0.08576007511005201 1101 78-79 0.4603993916407987 1101 80-81 0.397785196613178 1101 82-83 0.2900302325532067 1101 84-85 0.3657349379074617 1101 86-87 0.4896486099892918 1101 88-89 0.4719949948928317 1101 90-91 0.46041797865328604 1101 92-93 0.4743856365901493 1101 94-95 0.4151981979889712 1101 96-97 0.3475246418394029 1101 98-99 0.38085828215372786 1101 100-101 0.38132230925076627 2304 1 0.09358214215178862 2304 2 0.05216345268391365 2304 3 0.25930329189548473 2304 4 0.13749850406781405 2304 5 0.05061599578294107 2304 6 0.08808078026548571 2304 7 0.08050695475948544 2304 8 0.10304183916868226 2304 9 0.04217933338120616 2304 10-11 0.08130854304264545 2304 12-13 0.0722192732469864 2304 14-15 0.16467518817990623 2304 16-17 0.17965715059501264 2304 18-19 0.22371315369743883 2304 20-21 0.17173408888181996 2304 22-23 0.2298746251115773 2304 24-25 0.21696492631092212 2304 26-27 0.1933495927704243 2304 28-29 0.17727560588404145 2304 30-31 0.13808197531997024 2304 32-33 0.2111269577841881 2304 34-35 0.30050329358282823 2304 36-37 0.26837895970533765 2304 38-39 0.23928828259386847 2304 40-41 0.2599879744340612 2304 42-43 0.24128725326725942 2304 44-45 0.3212996902533476 2304 46-47 0.4093325917877664 2304 48-49 0.3053191667665871 2304 50-51 0.34948123658465136 2304 52-53 0.2578143052580302 2304 54-55 0.34728663517858394 2304 56-57 0.3119337339168311 2304 58-59 0.23429136141802331 2304 60-61 0.3306171811980576 2304 62-63 0.28242074174496423 2304 64-65 0.3003824345527164 2304 66-67 0.24838193438028355 2304 68-69 0.20591341006404207 2304 70-71 0.22495594334127844 2304 72-73 0.014676700803441634 2304 74-75 0.041685488091509626 2304 76-77 -0.02463310898019344 2304 78-79 0.3325360487481248 2304 80-81 0.3338615392748068 2304 82-83 0.38190707399333235 2304 84-85 0.32888944643416096 2304 86-87 0.36700207208637536 2304 88-89 0.40733851773416063 2304 90-91 0.37823803655356514 2304 92-93 0.4697714925191505 2304 94-95 0.39518411958716015 2304 96-97 0.3642655138438471 2304 98-99 0.33511387625802413 2304 100-101 0.2786438206288189 2202 1 0.13506913090409967 2202 2 0.0967442005049719 2202 3 0.2785644592101377 2202 4 0.1456165553236204 2202 5 0.09669886431635177 2202 6 0.10296858047052382 2202 7 0.12007897072017215 2202 8 0.11195689951002663 2202 9 0.10460800472930742 2202 10-11 0.12271184927340784 2202 12-13 0.13114423956687915 2202 14-15 0.09945918178443236 2202 16-17 0.04758109775928432 2202 18-19 0.08661365533948384 2202 20-21 0.09502225043426193 2202 22-23 0.16986183177711212 2202 24-25 0.09884866037377549 2202 26-27 0.14539767729193187 2202 28-29 0.13707529596810275 2202 30-31 0.13640642561367144 2202 32-33 0.1265320747793055 2202 34-35 0.10880723124706293 2202 36-37 0.10665467230995063 2202 38-39 0.18585240457200314 2202 40-41 0.1913904573404679 2202 42-43 0.2052469966884516 2202 44-45 0.20774481966743963 2202 46-47 0.2119278803679805 2202 48-49 0.12815397820128993 2202 50-51 0.2520411894936103 2202 52-53 0.13954685244094378 2202 54-55 0.18516139477725346 2202 56-57 0.16057248679143044 2202 58-59 0.21276657846284053 2202 60-61 0.2469866723218317 2202 62-63 0.173943503230646 2202 64-65 0.11008249964522321 2202 66-67 0.19504880028015492 2202 68-69 0.1363062565974431 2202 70-71 0.18595778307531674 2202 72-73 0.15201397468271693 2202 74-75 0.14588045605577094 2202 76-77 -0.03441677879262528 2202 78-79 0.17022776856283883 2202 80-81 0.16424603815059413 2202 82-83 0.1880120171384192 2202 84-85 0.23827479934305984 2202 86-87 0.2963186245532192 2202 88-89 0.3117897825850662 2202 90-91 0.2634200335668062 2202 92-93 0.28070800862882095 2202 94-95 0.2740250317682893 2202 96-97 0.26451963228474895 2202 98-99 0.28384843497928713 2202 100-101 0.16794266818983417 1102 1 0.12083004502142813 1102 2 0.09618495005105387 1102 3 0.24685327337390817 1102 4 0.1544335384457085 1102 5 0.07638171901999868 1102 6 0.05552165052716873 1102 7 0.09188079815935879 1102 8 0.11633195307680921 1102 9 0.052225494841778186 1102 10-11 0.07068400211178982 1102 12-13 0.11549602820919347 1102 14-15 0.10657729481154377 1102 16-17 0.1194886024595263 1102 18-19 0.05963601356635451 1102 20-21 0.08476688359218087 1102 22-23 0.08111015745754457 1102 24-25 0.13429352824881846 1102 26-27 0.06861119525226655 1102 28-29 0.12782876661191978 1102 30-31 0.0779650171631161 1102 32-33 0.1408176438737314 1102 34-35 0.055463577012822896 1102 36-37 0.06068312786706542 1102 38-39 0.13933553483992256 1102 40-41 0.12427833499401686 1102 42-43 -0.025397794314400812 1102 44-45 0.1413194741253676 1102 46-47 0.09732016699705781 1102 48-49 0.1516579843369854 1102 50-51 0.13702973434074295 1102 52-53 0.0686813372380044 1102 54-55 0.10700193109452982 1102 56-57 0.11309942241572912 1102 58-59 0.05939092054831008 1102 60-61 0.012127968086353746 1102 62-63 0.07697415583997724 1102 64-65 -0.016010328802551044 1102 66-67 0.0023278644527735537 1102 68-69 0.01577421059168671 1102 70-71 -0.1400465358816163 1102 72-73 -0.20670878369134726 1102 74-75 -0.23788859194164758 1102 76-77 -0.2653614778057971 1102 78-79 -0.08574898692745592 1102 80-81 -0.01776838189643115 1102 82-83 -0.023707903053889368 1102 84-85 -0.1467605146986699 1102 86-87 -0.024840309049313447 1102 88-89 0.027075908862066456 1102 90-91 -0.05965153130844314 1102 92-93 -0.027509189270777057 1102 94-95 -0.19427970944011008 1102 96-97 -0.1472299993253472 1102 98-99 -0.221746379421365 1102 100-101 -0.2854896011289263 2305 1 0.0889471384921734 2305 2 0.04107266495402229 2305 3 0.25951279739045674 2305 4 0.13249455704889357 2305 5 0.07744160281259127 2305 6 0.07302346835805906 2305 7 0.07691757360285578 2305 8 0.055235078854146025 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1213 96-97 0.03210924929999592 1213 98-99 0.006431906343426164 1213 100-101 0.2435991787552929 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 5.0 9 10.0 10 23.0 11 28.0 12 48.0 13 93.0 14 263.0 15 625.0 16 1308.0 17 2524.0 18 4030.0 19 5761.0 20 8248.0 21 11531.0 22 15469.0 23 20922.0 24 27900.0 25 37787.0 26 52713.0 27 81512.0 28 149302.0 29 222141.0 30 151230.0 31 98975.0 32 125774.0 33 179789.0 34 279312.0 35 494290.0 36 1007635.0 37 1732826.0 38 610851.0 39 408.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.95690192912038 24.30716896818469 22.98502311930003 12.750905983394901 2 26.777584644808055 29.86501126268073 32.037071511400846 11.320332581110367 3 34.5756957210586 18.35615553133511 32.464365323483854 14.603783424122438 4 25.36032687775079 18.40363374540032 33.62403872748263 22.61200064936627 5 36.8642164598758 17.430019876644952 31.688060844587408 14.017702818891847 6 30.495556073610274 18.002574702728534 37.331649175432005 14.170220048229181 7 29.90158609277308 26.61334543602664 28.33931298305028 15.14575548815 8 25.31235599952135 34.03959511832155 26.211736143502577 14.436312738654522 9 36.51332727071554 23.217390307914236 24.422669030849658 15.846613390520565 10-11 26.304310214788444 30.970110631858134 28.038318488931086 14.687260664422332 12-13 22.351694169019122 29.244660182320004 27.463341807035345 20.940303841625525 14-15 21.285226755493223 30.097816537120636 28.027562806985774 20.589393900400367 16-17 24.661316509788133 26.531366720811945 28.700167733260347 20.107149036139578 18-19 19.037706264101832 26.866870811951838 33.39379670593593 20.701626218010407 20-21 23.587928840822094 26.98255021806827 33.062697749699296 16.366823191410344 22-23 18.92088095935385 36.08908178391245 28.598154577216945 16.391882679516762 24-25 18.917546582188262 26.986091232692 33.06185241464323 21.034509770476504 26-27 18.959813334991445 27.1208282480168 28.263176472334155 25.656181944657604 28-29 23.005555825332312 31.67310733889107 28.236255810858296 17.085081024918324 30-31 18.028310458879805 27.77855527730465 32.45712789337056 21.736006370444983 32-33 17.837790722466547 32.33609469856573 28.373633586326463 21.452480992641267 34-35 22.636335168211343 32.283045227491876 28.619109493995587 16.461510110301198 36-37 18.016275120679857 32.32646948680217 33.26671711803168 16.390538274486296 38-39 18.402227263368765 27.81452083142349 32.650539965404946 21.1327119398028 40-41 22.788999835722834 27.649913244938006 32.40030353235353 17.160783386985624 42-43 18.297869502646 32.168203210370386 32.67840259567777 16.855524691305842 44-45 17.90628948722691 27.867141921502174 32.901303406643166 21.32526518462775 46-47 22.51728669844724 27.759475184702044 28.33767410684765 21.385564010003066 48-49 18.444999671247288 32.37983149075266 32.12491663770512 17.05025220029494 50-51 22.92573500825315 27.74037333928473 31.804061170510796 17.52983048195133 52-53 18.225929226681416 27.60192488037127 27.64923721655806 26.522908676389257 54-55 22.77300756872196 27.57013844851955 32.02812949139545 17.62872449136304 56-57 18.3792361786331 27.47201712915504 31.926018267078316 22.222728425133546 58-59 18.39340763976985 27.57904985360164 32.3586688421002 21.66887366452831 60-61 22.576562229804058 27.97378860399979 32.08068712534199 17.368962040854168 62-63 18.130937051578925 28.07224763565241 32.039219329231344 21.75759598353732 64-65 22.65298090010812 27.846445624064753 32.09513592927038 17.405437546556747 66-67 18.477992710803566 36.286562711910264 27.521000548089553 17.71444402919662 68-69 18.35266929682244 36.54152773449563 27.473108515862904 17.632694452819024 70-71 17.971826576870274 37.02581903003514 27.574217873620388 17.428136519474194 72-73 17.854204666251107 36.88333808548327 27.49247602356377 17.769981224701848 74-75 18.36273327427697 35.9299906830743 27.48011122438233 18.2271648182664 76-77 18.871314489245243 34.844971585616456 27.83171229155434 18.45200163358396 78-79 18.85610983440837 34.216592643167345 28.26975898547333 18.657538536950955 80-81 18.424859977398757 34.18973904308879 28.117141355080822 19.26825962443162 82-83 18.05982558853818 33.81094980269661 28.25691482347142 19.872309785293787 84-85 18.136318079903212 33.19587493295686 28.880992435748954 19.786814551390975 86-87 18.078699324219592 32.976576991602016 29.433337585651486 19.511386098526902 88-89 18.36964600685846 33.268924087210536 28.999134989053964 19.362294916877033 90-91 18.750850817625757 32.689716711669384 28.764686556672835 19.794745914032024 92-93 18.34222676763701 33.24540110268689 28.640810538564637 19.771561591111464 94-95 18.118942833918513 33.35626330091571 28.51419447042673 20.01059939473905 96-97 18.600328321388552 33.49067944376085 28.196161178311698 19.712831056538903 98-99 18.8795095901328 32.6903456194808 28.085781513211423 20.34436327717499 100-101 18.765773280758786 32.75242157996661 27.81679803257872 20.66500710669589 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 442.0 1 330.5 2 281.0 3 629.0 4 738.5 5 485.0 6 401.0 7 384.0 8 373.0 9 373.5 10 368.5 11 352.5 12 372.5 13 400.0 14 414.5 15 480.0 16 609.5 17 786.5 18 1103.0 19 1697.0 20 2641.0 21 4096.0 22 6442.5 23 9967.0 24 14431.5 25 20436.0 26 28621.5 27 38855.0 28 50766.0 29 63002.5 30 76020.5 31 89722.0 32 105745.0 33 127114.5 34 154705.5 35 193896.5 36 252014.5 37 324204.5 38 388943.5 39 410715.5 40 409499.5 41 382159.5 42 330067.0 43 304009.5 44 275532.5 45 245257.0 46 238280.5 47 209245.5 48 147821.0 49 99747.5 50 73286.0 51 56779.5 52 44644.5 53 33222.5 54 25007.5 55 19290.5 56 14406.5 57 10712.5 58 8245.5 59 6388.0 60 4737.5 61 3445.5 62 2539.5 63 1924.5 64 1389.5 65 895.5 66 549.0 67 344.5 68 222.0 69 156.0 70 113.0 71 82.5 72 55.0 73 37.5 74 24.0 75 19.5 76 17.5 77 8.0 78 3.5 79 8.0 80 7.0 81 1.5 82 2.0 83 2.0 84 1.0 85 0.5 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16797746825156346 2 0.0 3 1.314965642765538E-4 4 0.0229367578545246 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.757044693615823E-5 12-13 9.392611734039557E-6 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 2.8177835202118672E-5 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0010237946790103117 38-39 5.447714805742943E-4 40-41 2.3481529335098893E-4 42-43 4.132749162977405E-4 44-45 2.5360051681906805E-4 46-47 0.0030995618722330543 48-49 0.003437695894658478 50-51 0.003991859986966812 52-53 0.003700689023211586 54-55 0.005776456216434328 56-57 0.008988729429475857 58-59 0.01632435919376075 60-61 0.004292423562456078 62-63 0.002658109120733195 64-65 0.009918597991145773 66-67 0.02273012039637573 68-69 0.025200377282428132 70-71 0.025679400480864152 72-73 0.022175956304067396 74-75 0.02439261267330073 76-77 0.024279901332492256 78-79 0.015244208844346203 80-81 0.0032780214951798055 82-83 0.0011083281846166677 84-85 0.005034439889445202 86-87 0.01401377670718702 88-89 0.0160989365121438 90-91 0.023415781052960615 92-93 0.02333124754735426 94-95 0.0250031324360133 96-97 0.026552913372129828 98-99 0.03275203711659594 100-101 0.023284284488684064 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5323333.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.157379921296574 #Duplication Level Percentage of deduplicated Percentage of total 1 93.50480907768372 49.70470660610734 2 3.3811714356282336 3.594684291654515 3 0.9733836389509816 1.5522757171467452 4 0.45487838068233283 0.9672057159965975 5 0.26736557133838373 0.7106226626754493 6 0.22947339126860195 0.7318922544896047 7 0.16082512817516167 0.5984329707508801 8 0.11413804203189107 0.4853823411009721 9 0.07130047939665721 0.3411132004672817 >10 0.6434846945868272 7.011634095676 >50 0.08376939444250017 3.2042887520185492 >100 0.09130970329213052 10.106211482716978 >500 0.015675063478984515 5.783573211248964 >1k 0.008167597698361425 6.448736998036221 >5k 2.129158075209936E-4 0.7610618573764405 >10k+ 3.5485537715881414E-5 7.9981778425373395 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT 424010 7.965122602700225 TruSeq Adapter, Index 7 (97% over 35bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 8196 0.15396369154437642 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 7518 0.14122731003301878 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 6597 0.12392611921891793 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 6584 0.1236819113138329 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 6018 0.11304947483090012 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 5433 0.10206011910207384 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0015403883243824874 0.0 0.0 2 0.0017094553355951995 0.0 0.0 3 0.0018973075702759907 0.0 0.0 4 0.002085159804956782 0.0 1.8785223468079115E-5 5 0.002460864274318364 0.0 1.8785223468079115E-5 6 0.0026675017324672345 0.0 1.8785223468079115E-5 7 0.0029868505314245794 0.0 1.8785223468079115E-5 8 0.0034940515650627156 0.0 5.6355670404237344E-5 9 0.003719474246679665 0.0 5.6355670404237344E-5 10-11 0.006875391789316955 0.0 7.514089387231646E-5 12-13 0.009232937334560886 0.0 7.514089387231646E-5 14-15 0.011609268103272893 0.0 7.514089387231646E-5 16-17 0.012999374639910747 0.0 7.514089387231646E-5 18-19 0.01465247430510171 0.0 9.392611734039557E-5 20-21 0.016183470017750157 0.0 9.392611734039557E-5 22-23 0.017855354906409198 0.0 9.392611734039557E-5 24-25 0.019790232923621347 0.0 9.392611734039557E-5 26-27 0.021772073999503695 0.0 9.392611734039557E-5 28-29 0.024308079167694376 0.0 9.392611734039557E-5 30-31 0.04121478028896558 0.0 1.0331872907443513E-4 32-33 0.044304949549464595 0.0 1.1271134080847469E-4 34-35 0.04689731038805951 1.8785223468079115E-5 1.1271134080847469E-4 36-37 0.04885097362873974 1.8785223468079115E-5 1.1271134080847469E-4 38-39 0.051283660067855986 1.8785223468079115E-5 1.2210395254251425E-4 40-41 0.05411083619980189 1.8785223468079115E-5 1.5967439947867248E-4 42-43 0.057191612848566865 1.8785223468079115E-5 1.6906701121271203E-4 44-45 0.060958050153916726 1.8785223468079115E-5 1.6906701121271203E-4 46-47 0.06501565842302182 1.8785223468079115E-5 1.784596229467516E-4 48-49 0.06923294109160558 1.8785223468079115E-5 2.0663745814887026E-4 50-51 0.07414527702850826 1.8785223468079115E-5 2.0663745814887026E-4 52-53 0.07908579080061308 1.8785223468079115E-5 2.0663745814887026E-4 54-55 0.08491860268745163 1.8785223468079115E-5 2.0663745814887026E-4 56-57 0.09185974275890688 1.8785223468079115E-5 2.2542268161694938E-4 58-59 0.09977771445070222 1.8785223468079115E-5 2.2542268161694938E-4 60-61 0.10828742068174206 1.8785223468079115E-5 2.2542268161694938E-4 62-63 0.11820601867288782 1.8785223468079115E-5 2.2542268161694938E-4 64-65 0.12881966993235255 1.8785223468079115E-5 2.2542268161694938E-4 66-67 0.14023169318921058 1.8785223468079115E-5 2.2542268161694938E-4 68-69 0.15330620872299366 1.8785223468079115E-5 2.2542268161694938E-4 70-71 0.1687758402489568 1.8785223468079115E-5 2.2542268161694938E-4 72-73 0.1870538626833978 1.8785223468079115E-5 2.2542268161694938E-4 74-75 0.20729494097025303 1.8785223468079115E-5 2.2542268161694938E-4 76-77 0.22966814212073527 1.8785223468079115E-5 2.3481529335098893E-4 78-79 0.2541546809113764 1.8785223468079115E-5 2.629931285531076E-4 80-81 0.28256733140684603 1.8785223468079115E-5 2.817783520211867E-4 82-83 0.31463370786685707 1.8785223468079115E-5 2.817783520211867E-4 84-85 0.3504383437970159 1.8785223468079115E-5 3.0056357548926584E-4 86-87 0.39156859058037513 1.8785223468079115E-5 3.0056357548926584E-4 88-89 0.43623045937573324 1.8785223468079115E-5 3.0056357548926584E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCGGA 50555 0.0 88.42485 1 TCGGAAG 50510 0.0 88.26382 3 ATCGGAA 50710 0.0 87.95317 2 CGGAAGA 51190 0.0 87.89785 4 GAAGAGC 52530 0.0 85.65362 6 GGAAGAG 54940 0.0 82.008705 5 GAGCACA 70805 0.0 63.445522 9 AGAGCAC 70890 0.0 63.42974 8 AAGAGCA 72580 0.0 61.887375 7 GCCGTCT 48115 0.0 46.410957 50-51 ATGCCGT 47850 0.0 46.40751 48-49 TGCCGTC 48060 0.0 46.31583 50-51 TCGTATG 48130 0.0 46.313633 44-45 TATGCCG 47840 0.0 46.303043 48-49 TCTCGTA 47825 0.0 46.266388 42-43 TATCTCG 47535 0.0 46.25869 40-41 CGTATGC 48010 0.0 46.221653 46-47 CGTATCT 47575 0.0 46.169666 38-39 CGTCTTC 48345 0.0 46.14985 52-53 CTCGTAT 47895 0.0 46.13431 44-45 >>END_MODULE