FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n01_52370180-2.341000000358c0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n01_52370180-2.341000000358c0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5323333
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT4240107.965122602700225TruSeq Adapter, Index 7 (97% over 35bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC81960.15396369154437642No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC75180.14122731003301878No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG65970.12392611921891793No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA65840.1236819113138329No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG60180.11304947483090012No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA54330.10206011910207384No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA505550.088.424851
TCGGAAG505100.088.263823
ATCGGAA507100.087.953172
CGGAAGA511900.087.897854
GAAGAGC525300.085.653626
GGAAGAG549400.082.0087055
GAGCACA708050.063.4455229
AGAGCAC708900.063.429748
AAGAGCA725800.061.8873757
GCCGTCT481150.046.41095750-51
ATGCCGT478500.046.4075148-49
TGCCGTC480600.046.3158350-51
TCGTATG481300.046.31363344-45
TATGCCG478400.046.30304348-49
TCTCGTA478250.046.26638842-43
TATCTCG475350.046.2586940-41
CGTATGC480100.046.22165346-47
CGTATCT475750.046.16966638-39
CGTCTTC483450.046.1498552-53
CTCGTAT478950.046.1343144-45
Produced by FastQC (version 0.11.2)