##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n01_52260024-1.34100000035820.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6634688 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.662059466850586 33.0 33.0 33.0 33.0 33.0 2 32.713298650968966 33.0 33.0 33.0 33.0 33.0 3 32.5497557684702 33.0 33.0 33.0 33.0 33.0 4 36.59941296410622 37.0 37.0 37.0 37.0 37.0 5 36.667632750778935 37.0 37.0 37.0 37.0 37.0 6 36.54284361223919 37.0 37.0 37.0 37.0 37.0 7 36.46464596375896 37.0 37.0 37.0 37.0 37.0 8 36.40638987093289 37.0 37.0 37.0 37.0 37.0 9 36.4327219305565 37.0 37.0 37.0 37.0 37.0 10-11 36.381844104801914 37.0 37.0 37.0 37.0 37.0 12-13 36.35984126156347 37.0 37.0 37.0 37.0 37.0 14-15 38.1871612500844 40.0 37.0 40.0 37.0 40.0 16-17 38.18426450497748 40.0 37.0 40.0 37.0 40.0 18-19 38.19105751468645 40.0 37.0 40.0 37.0 40.0 20-21 38.13732710566043 40.0 37.0 40.0 37.0 40.0 22-23 38.002486251049035 40.0 37.0 40.0 33.0 40.0 24-25 37.99930170039646 40.0 37.0 40.0 35.0 40.0 26-27 37.93362890613696 40.0 37.0 40.0 33.0 40.0 28-29 37.78670737795055 40.0 37.0 40.0 33.0 40.0 30-31 37.74935347374285 40.0 37.0 40.0 33.0 40.0 32-33 37.62924081735268 40.0 37.0 40.0 33.0 40.0 34-35 37.51307619891094 40.0 37.0 40.0 33.0 40.0 36-37 37.36637985086865 40.0 37.0 40.0 33.0 40.0 38-39 37.32063120375819 40.0 37.0 40.0 33.0 40.0 40-41 37.28257613621017 40.0 37.0 40.0 33.0 40.0 42-43 37.06967471567616 38.5 37.0 40.0 33.0 40.0 44-45 36.95188515270047 37.0 37.0 40.0 33.0 40.0 46-47 36.92262740915624 37.0 37.0 40.0 33.0 40.0 48-49 36.698773099805145 37.0 37.0 40.0 33.0 40.0 50-51 36.539741054892104 37.0 37.0 40.0 33.0 40.0 52-53 36.40466258549007 37.0 37.0 40.0 33.0 40.0 54-55 36.23252434779148 37.0 37.0 40.0 33.0 40.0 56-57 36.05106336575284 37.0 37.0 40.0 33.0 40.0 58-59 35.824683391291344 37.0 37.0 40.0 30.0 40.0 60-61 35.48128645989081 37.0 33.0 40.0 27.0 40.0 62-63 35.227569631006006 37.0 33.0 38.5 27.0 40.0 64-65 34.928435444138444 37.0 33.0 37.0 27.0 40.0 66-67 34.49955725122267 37.0 33.0 37.0 27.0 40.0 68-69 34.22731453234877 37.0 33.0 37.0 27.0 40.0 70-71 33.86984858971515 37.0 33.0 37.0 27.0 37.0 72-73 33.34979504688088 37.0 33.0 37.0 27.0 37.0 74-75 32.8502996674448 37.0 33.0 37.0 24.5 37.0 76-77 30.26582960645625 33.0 30.0 35.0 22.0 37.0 78-79 32.56942602274591 33.0 33.0 37.0 27.0 37.0 80-81 32.951270655078275 37.0 33.0 37.0 27.0 37.0 82-83 32.89474915775995 37.0 33.0 37.0 27.0 37.0 84-85 32.74041522374526 37.0 33.0 37.0 27.0 37.0 86-87 32.59989090670126 37.0 33.0 37.0 24.5 37.0 88-89 32.49791633005199 33.0 33.0 37.0 22.0 37.0 90-91 32.37824890936846 33.0 33.0 37.0 22.0 37.0 92-93 32.21000799133282 33.0 33.0 37.0 22.0 37.0 94-95 32.09005540275594 33.0 33.0 37.0 22.0 37.0 96-97 31.950693838203094 33.0 33.0 37.0 22.0 37.0 98-99 31.775471808169428 33.0 33.0 37.0 22.0 37.0 100-101 30.1036122572757 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 0.12829736690252247 2306 2 0.12163386828623146 2306 3 0.3030329435289687 2306 4 0.21396854943391475 2306 5 0.1347618654653715 2306 6 0.18299564825006343 2306 7 0.17987325519046493 2306 8 0.19994139832518698 2306 9 0.19199196741246993 2306 10-11 0.16069754069996378 2306 12-13 0.1937717403728314 2306 14-15 0.33966714332959924 2306 16-17 0.37239375658538165 2306 18-19 0.37134209597105894 2306 20-21 0.37183634710445546 2306 22-23 0.4575796871927835 2306 24-25 0.41255891706049397 2306 26-27 0.444435433881587 2306 28-29 0.48307468444814816 2306 30-31 0.4514171748156528 2306 32-33 0.49085105545440655 2306 34-35 0.4500117161679995 2306 36-37 0.4243756051907681 2306 38-39 0.40714466032061125 2306 40-41 0.49214979713222107 2306 42-43 0.5096426141040595 2306 44-45 0.5195134291722283 2306 46-47 0.5118337059112221 2306 48-49 0.4941327102265731 2306 50-51 0.47885559270604716 2306 52-53 0.5144596663080634 2306 54-55 0.5601340526328968 2306 56-57 0.5623799280089301 2306 58-59 0.5429776510043425 2306 60-61 0.5372552133499937 2306 62-63 0.616754420584833 2306 64-65 0.4179175223795184 2306 66-67 0.35177083915372265 2306 68-69 0.42843509152057635 2306 70-71 0.5087958402017563 2306 72-73 0.4348022678737351 2306 74-75 0.40186323002824764 2306 76-77 0.20498469579682776 2306 78-79 0.4301788073708721 2306 80-81 0.5968230614910794 2306 82-83 0.5455843782965886 2306 84-85 0.6163452584735225 2306 86-87 0.6425477256610534 2306 88-89 0.6493750597921562 2306 90-91 0.6194069889335196 2306 92-93 0.5903465085224155 2306 94-95 0.605339854163887 2306 96-97 0.594438445090443 2306 98-99 0.4235132068253584 2306 100-101 0.46442154136629554 2307 1 0.1418268462043173 2307 2 0.1225843084626419 2307 3 0.32493575249174 2307 4 0.19309804531116015 2307 5 0.1524279638827437 2307 6 0.1756624126454298 2307 7 0.15471182757666924 2307 8 0.1662243733883244 2307 9 0.1941809708772695 2307 10-11 0.15762967995681976 2307 12-13 0.198736586860619 2307 14-15 0.4076608483965387 2307 16-17 0.36170477080210617 2307 18-19 0.3393759700265946 2307 20-21 0.3622399936416585 2307 22-23 0.39447492270051754 2307 24-25 0.3311664357417641 2307 26-27 0.41848425762425023 2307 28-29 0.46744347985050894 2307 30-31 0.4065517561164498 2307 32-33 0.48943431384250147 2307 34-35 0.43076541943406 2307 36-37 0.39663287023178384 2307 38-39 0.37597260603475746 2307 40-41 0.4061155270729344 2307 42-43 0.45125094862783754 2307 44-45 0.4284754054895856 2307 46-47 0.42265668179377514 2307 48-49 0.4410919637454711 2307 50-51 0.377374644583945 2307 52-53 0.4005266432867316 2307 54-55 0.4997118942441645 2307 56-57 0.44147758156982775 2307 58-59 0.3871732282561524 2307 60-61 0.3778493295940777 2307 62-63 0.4759440055925239 2307 64-65 0.4148754439424138 2307 66-67 0.3096678270793447 2307 68-69 0.37199283321488963 2307 70-71 0.2985970609588975 2307 72-73 0.19113365447418573 2307 74-75 0.13098170416439103 2307 76-77 -0.08955123905167639 2307 78-79 0.38136885163076073 2307 80-81 0.4087936381937496 2307 82-83 0.46174600515854536 2307 84-85 0.4837779763912593 2307 86-87 0.46912732810256585 2307 88-89 0.5130734326716038 2307 90-91 0.42706989533003537 2307 92-93 0.4320207164486902 2307 94-95 0.4786716182297681 2307 96-97 0.6054272767562914 2307 98-99 0.390797758270093 2307 100-101 0.26684191571291294 2201 1 0.1228659356483206 2201 2 0.10781464562744247 2201 3 0.2760561666145023 2201 4 0.2056490055878868 2201 5 0.1530464608110691 2201 6 0.1429142051546819 2201 7 0.20885856834613037 2201 8 0.17387431142909548 2201 9 0.18325015125565614 2201 10-11 0.19660926225598274 2201 12-13 0.19922484892806835 2201 14-15 0.3410777189773242 2201 16-17 0.3244753449527309 2201 18-19 0.30896023347853685 2201 20-21 0.3158211419721155 2201 22-23 0.3869894668711993 2201 24-25 0.30083361575357515 2201 26-27 0.37094072004088474 2201 28-29 0.4681575991787881 2201 30-31 0.4211409083959481 2201 32-33 0.45849270886122895 2201 34-35 0.38211421007905955 2201 36-37 0.38377065338399063 2201 38-39 0.38667932092519663 2201 40-41 0.4003775623878667 2201 42-43 0.4815476592026826 2201 44-45 0.44080594864964695 2201 46-47 0.5147027893888847 2201 48-49 0.4399456998101172 2201 50-51 0.416813681744145 2201 52-53 0.4463499269577582 2201 54-55 0.45718752860513945 2201 56-57 0.4231855896811183 2201 58-59 0.4621195022685569 2201 60-61 0.4031481176467935 2201 62-63 0.37921137809050975 2201 64-65 0.4178448863970132 2201 66-67 0.41058695586764316 2201 68-69 0.45460072879917135 2201 70-71 0.3582202378716559 2201 72-73 0.3956101994203678 2201 74-75 0.35165534735957493 2201 76-77 0.15684300418045538 2201 78-79 0.3784892263216051 2201 80-81 0.4634435740438647 2201 82-83 0.5450324583232842 2201 84-85 0.5712755392796893 2201 86-87 0.5895211507275775 2201 88-89 0.5738022002270213 2201 90-91 0.5174852375589154 2201 92-93 0.5199113206259938 2201 94-95 0.618529035336941 2201 96-97 0.6323521202517348 2201 98-99 0.453971706332414 2201 100-101 0.4228182609437354 1101 1 0.12355807928561546 1101 2 0.10164254981650345 1101 3 0.28954642640415074 1101 4 0.18942728840234224 1101 5 0.11988441401303618 1101 6 0.1602208384767536 1101 7 0.1263240144060802 1101 8 0.1292737923974272 1101 9 0.1454658019163091 1101 10-11 0.10290655239955271 1101 12-13 0.17090126645344839 1101 14-15 0.2837924128064486 1101 16-17 0.23180126702496295 1101 18-19 0.24230771392358008 1101 20-21 0.24592756476082656 1101 22-23 0.2588225674617348 1101 24-25 0.27867741338598506 1101 26-27 0.23160140536956675 1101 28-29 0.23680075425851754 1101 30-31 0.28320322262003117 1101 32-33 0.308479170227443 1101 34-35 0.3281699040633512 1101 36-37 0.3069051361321158 1101 38-39 0.34022492178193886 1101 40-41 0.3182592073470758 1101 42-43 0.3350731802879565 1101 44-45 0.2824699532254087 1101 46-47 0.32617062068228364 1101 48-49 0.2817148189470515 1101 50-51 0.2247757674847648 1101 52-53 0.24656715357622971 1101 54-55 0.30169312508083834 1101 56-57 0.3864577101354101 1101 58-59 0.36042808576237206 1101 60-61 0.31959299411056463 1101 62-63 0.29191350685052697 1101 64-65 0.1927536907683205 1101 66-67 0.23866653869517762 1101 68-69 0.23840228589417478 1101 70-71 0.2033651925248421 1101 72-73 0.15914137935033068 1101 74-75 0.136488274533896 1101 76-77 -0.11493472491908818 1101 78-79 0.19336742425338826 1101 80-81 0.2872312578422296 1101 82-83 0.3170318255062412 1101 84-85 0.3641231855349645 1101 86-87 0.32814732927325707 1101 88-89 0.4287404549297946 1101 90-91 0.4004134767150802 1101 92-93 0.4516828432507225 1101 94-95 0.29860320879574687 1101 96-97 0.4204810202149254 1101 98-99 0.32640811278350057 1101 100-101 0.34349510136127037 2304 1 0.12075483816975208 2304 2 0.08184639388490922 2304 3 0.2874321386638812 2304 4 0.18596321635097723 2304 5 0.092987295551481 2304 6 0.09688390584234696 2304 7 0.13717064464165674 2304 8 0.16627883977213997 2304 9 0.10069747606280544 2304 10-11 0.11276100809853773 2304 12-13 0.07659141062411123 2304 14-15 0.21904810518741868 2304 16-17 0.21192970025978752 2304 18-19 0.1818000101384456 2304 20-21 0.17323783249573665 2304 22-23 0.27659864118670185 2304 24-25 0.23880113912852607 2304 26-27 0.24013856142423862 2304 28-29 0.25313901022544627 2304 30-31 0.22487398699537664 2304 32-33 0.29927399020392187 2304 34-35 0.32514437501341575 2304 36-37 0.300025811842886 2304 38-39 0.2566819695345828 2304 40-41 0.2947521887099782 2304 42-43 0.27516811347813785 2304 44-45 0.28167486780279205 2304 46-47 0.3187571092513082 2304 48-49 0.3109895829830762 2304 50-51 0.260578835782205 2304 52-53 0.276682249535007 2304 54-55 0.36097114500859817 2304 56-57 0.3956154161387744 2304 58-59 0.31653206602505435 2304 60-61 0.4127065788826414 2304 62-63 0.28290852518765774 2304 64-65 0.1642794881415881 2304 66-67 0.06030632012348747 2304 68-69 0.1673733554211978 2304 70-71 0.14317175468740118 2304 72-73 0.09568128342218074 2304 74-75 0.028227228865468135 2304 76-77 -0.07390598749784871 2304 78-79 0.17355112782838944 2304 80-81 0.2230581234784026 2304 82-83 0.3024448643317328 2304 84-85 0.2258090432146247 2304 86-87 0.2864674599098791 2304 88-89 0.3316665252113964 2304 90-91 0.24879189783776212 2304 92-93 0.3442177647315674 2304 94-95 0.3311295023490928 2304 96-97 0.2685303716631928 2304 98-99 0.21660664421668585 2304 100-101 0.07715341130100484 2202 1 0.10707223662144827 2202 2 0.08931857341909932 2202 3 0.265475326134613 2202 4 0.17349344703114866 2202 5 0.07574361944583785 2202 6 0.08095863759441357 2202 7 0.05599225581845957 2202 8 0.1324886300491528 2202 9 0.11672660326306783 2202 10-11 0.10380664973056497 2202 12-13 0.08161543723397813 2202 14-15 0.07112119854306087 2202 16-17 -0.017277136646299596 2202 18-19 0.008771253053723171 2202 20-21 0.0543397605299063 2202 22-23 0.14785551304431976 2202 24-25 0.10590382456052794 2202 26-27 0.10377810871593596 2202 28-29 0.16662793628611183 2202 30-31 0.15088323851051655 2202 32-33 0.1712086061008975 2202 34-35 0.13128391543841644 2202 36-37 0.07237574338181219 2202 38-39 0.1351294000812686 2202 40-41 0.11127427684828461 2202 42-43 0.08897004123762287 2202 44-45 0.1621793064425816 2202 46-47 0.10882357554744715 2202 48-49 0.169500643875665 2202 50-51 0.07218513378906977 2202 52-53 0.08110863224957399 2202 54-55 0.1943963205559811 2202 56-57 0.10901140131218057 2202 58-59 0.04906015772582606 2202 60-61 0.13153138307847456 2202 62-63 0.17840049731980656 2202 64-65 0.08121541557971312 2202 66-67 -0.029116752838028503 2202 68-69 -0.06265988974360681 2202 70-71 0.11846757245047712 2202 72-73 -0.04893544099348901 2202 74-75 -0.1516324430981726 2202 76-77 -0.34025776412110176 2202 78-79 0.022427597132143262 2202 80-81 0.0855947058631088 2202 82-83 0.10214102968754446 2202 84-85 0.1296190015886438 2202 86-87 0.19560538253856663 2202 88-89 0.2797149897468998 2202 90-91 0.2257647372625513 2202 92-93 0.19789955600838027 2202 94-95 0.2675345199896668 2202 96-97 0.2420265112001836 2202 98-99 0.05560003295061122 2202 100-101 0.20416715913627215 1102 1 0.12226874328571569 1102 2 0.10458869581381691 1102 3 0.2557438488970547 1102 4 0.16197952239537727 1102 5 0.047548957908439604 1102 6 0.02703666569831853 1102 7 0.02718043003082471 1102 8 0.1765341784357446 1102 9 0.08193966122460239 1102 10-11 0.08976432672498191 1102 12-13 0.0874351606346977 1102 14-15 0.1119583416128691 1102 16-17 0.08548201684135392 1102 18-19 0.0639797047272026 1102 20-21 0.0948071926695846 1102 22-23 0.15633266637789234 1102 24-25 0.08960251064216607 1102 26-27 0.08915453157252529 1102 28-29 0.11721709064866559 1102 30-31 0.09905784588240607 1102 32-33 0.12672846374015023 1102 34-35 0.12037646363305043 1102 36-37 0.12838425603718662 1102 38-39 0.17195505713839054 1102 40-41 0.15642781543076723 1102 42-43 0.10815216231085145 1102 44-45 0.14724812654075237 1102 46-47 0.20900585201352584 1102 48-49 0.111241301696694 1102 50-51 0.15229496323562586 1102 52-53 0.03614182625167217 1102 54-55 0.08977367707549178 1102 56-57 0.061982538722553215 1102 58-59 0.07467720771661135 1102 60-61 0.11695742718131896 1102 62-63 0.13658823147006416 1102 64-65 -0.021024836783496426 1102 66-67 -0.05678745318968481 1102 68-69 0.052373340168607285 1102 70-71 0.04692108060729083 1102 72-73 -0.04632159287891113 1102 74-75 -0.02152488251781648 1102 76-77 -0.19053879339633184 1102 78-79 0.009132694148213716 1102 80-81 0.002426069363544059 1102 82-83 0.08167757051212021 1102 84-85 -0.03440223238728635 1102 86-87 0.031029908623018798 1102 88-89 0.2094234747188537 1102 90-91 0.09893283184587176 1102 92-93 0.22457358751265133 1102 94-95 -0.04096076795067205 1102 96-97 -0.023766184944111757 1102 98-99 -0.20604251517872996 1102 100-101 -0.2055428210021688 2305 1 0.07015102018366548 2305 2 0.04113526858919414 2305 3 0.2247479793438103 2305 4 0.12677296071430533 2305 5 0.03763003121984809 2305 6 0.02898534399544417 2305 7 0.03287923705953233 2305 8 0.022415334224447747 2305 9 0.04984297265389159 2305 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94-95 0.07411618252601926 1213 96-97 0.1372741851789776 1213 98-99 0.07729086362358117 1213 100-101 0.32835026344936225 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 5.0 8 107.0 9 303.0 10 252.0 11 110.0 12 92.0 13 213.0 14 514.0 15 1176.0 16 2222.0 17 3736.0 18 5867.0 19 8211.0 20 11292.0 21 15233.0 22 20330.0 23 26105.0 24 34431.0 25 44251.0 26 57838.0 27 79576.0 28 118603.0 29 164951.0 30 157583.0 31 159883.0 32 211010.0 33 301617.0 34 466062.0 35 809619.0 36 1523171.0 37 2022872.0 38 387107.0 39 346.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.43026554364056 25.83510598948937 21.880303814896955 15.854324651973121 2 29.565534958086953 25.064434077382387 31.07482974331272 14.295201221217937 3 35.721786339517244 19.667022624284346 27.149729037625754 17.461461998572652 4 28.760345180827056 19.28213411373552 31.709451949078275 20.248068756359146 5 33.85701030704082 17.958915325031107 30.696424609567174 17.487649758360906 6 28.839864059922636 19.01625215835319 34.75557554477317 17.388308236951005 7 31.810086623515684 21.916780412281632 27.472881919993824 18.80025104420886 8 28.455264211369098 27.820569708779068 25.220070634821113 18.504095445030725 9 31.093052152565427 23.3990204211562 25.917556334224006 19.590371092054365 10-11 24.99057415190164 28.473944077044237 29.102112889669236 17.433368881384887 12-13 23.742241062597145 27.073143987565352 28.534659695727942 20.649955254109557 14-15 23.378002100475562 27.493787198433445 28.571629894276867 20.556580806814125 16-17 24.356096322841406 26.322496250012055 29.26313189105501 20.058275536091525 18-19 21.935839334117897 26.376281748290197 31.010923196388436 20.67695572120347 20-21 23.414235906797728 26.68353055938727 30.51445674611979 19.387776787695216 22-23 21.94786702856261 29.908595551139705 28.650156571039965 19.49338084925772 24-25 21.82387476246057 26.201940468037083 30.723728078848623 21.250456690653728 26-27 21.667590096173324 26.36469868666017 29.173225628695725 22.794485588470774 28-29 22.832151676865973 28.449540961524388 28.62702493645703 20.09128242515261 30-31 20.848433264684036 27.50827167758303 30.163053635679628 21.480241422053307 32-33 20.68014351240028 28.974768670357975 28.945242037003094 21.39984578023865 34-35 22.52108162433561 29.16970624692525 29.067855187764668 19.241356940974466 36-37 22.211559280400646 29.44833896378362 29.437215473980118 18.90288628183562 38-39 21.02646038312922 27.58399853375937 32.003286682072144 19.38625440103927 40-41 21.016632863158986 27.28869642505769 28.664085828579328 23.030584883204 42-43 21.278264770691386 28.95336385842781 30.18170286437846 19.58666850650234 44-45 20.885752132379224 27.55428626291917 30.4274977498869 21.132463854814706 46-47 22.489727481513537 27.462242908190266 28.97981429957484 21.068215310721357 48-49 21.056076136299833 29.27080636694932 30.033307370240113 19.639810126510728 50-51 22.45059763367257 27.819868748722094 29.444114052366814 20.285419565238527 52-53 20.75507418197697 27.49469450452487 28.368074886110556 23.38215642738761 54-55 22.51164879920805 27.573081730575378 29.81770783285419 20.097561637362375 56-57 21.187717670144764 27.276659474713316 29.49224939966773 22.043373455474192 58-59 21.164199869617022 27.433904202955457 29.604541892410445 21.797354035017076 60-61 22.35084212304934 27.67304864084163 29.481036838050617 20.49507239805841 62-63 20.8058060856853 27.727117409379204 29.52054167865579 21.946534826279713 64-65 22.716790870751076 27.619176680523623 29.440068652665808 20.223963796059497 66-67 21.666049564370002 30.498471918026304 27.689136326138765 20.14634219146493 68-69 21.738921662779532 30.378697992911192 27.71405504908043 20.168325295228847 70-71 21.426009551375998 30.95467325275506 27.74617889931978 19.873138296549165 72-73 21.34974062468223 31.238845323604618 27.45166944475915 19.959744606953997 74-75 21.93521478733839 30.515619869423094 27.22020839357482 20.3289569496637 76-77 22.060139360204627 29.55655087790235 27.759859864885218 20.623449897007806 78-79 21.757761834728953 29.28042821919642 28.455183993275458 20.50662595279917 80-81 21.335209799809498 29.5586427552344 28.27643504429263 20.829712400663475 82-83 20.511899159068566 29.601849092052547 28.54514754640723 21.341104202471662 84-85 20.65013100836877 29.571482124212185 28.487797297352298 21.290589570066754 86-87 20.483615611401067 29.65916718140127 29.028611957696164 20.828605249501503 88-89 20.605212028323557 29.961704572032556 28.868369990500575 20.564713409143312 90-91 21.017140207235904 29.383033647680268 28.399453831434002 21.200372313649826 92-93 20.60792116121612 29.947322364020764 28.0268044387521 21.41795203601102 94-95 20.36637547633018 30.108522473377068 28.027180444938672 21.497921605354076 96-97 20.72866484565032 30.56236418135827 27.63974752620488 21.06922344678653 98-99 20.938980160617803 30.012597031442823 27.65204451101882 21.39637829692055 100-101 21.058448095406263 29.920305075713006 27.239841959443595 21.78140486943714 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 29.0 1 23.0 2 28.5 3 63.0 4 81.5 5 63.0 6 47.5 7 47.0 8 52.0 9 52.0 10 51.0 11 52.0 12 59.5 13 68.0 14 65.0 15 69.0 16 93.5 17 133.5 18 154.0 19 182.0 20 293.0 21 456.0 22 679.0 23 1003.0 24 1477.5 25 2280.0 26 3694.0 27 5743.5 28 8255.5 29 10677.0 30 13443.5 31 17582.0 32 29162.5 33 52501.5 34 89762.0 35 143335.5 36 218703.0 37 314348.5 38 404959.5 39 441080.5 40 449620.5 41 440211.0 42 417322.5 43 426850.0 44 424877.0 45 409147.5 46 407369.5 47 369885.5 48 296829.0 49 243404.0 50 202438.5 51 171740.0 52 150080.5 53 121919.0 54 88986.0 55 60750.0 56 43480.5 57 32548.0 58 26002.0 59 20942.5 60 16119.0 61 12553.5 62 9662.0 63 7474.0 64 5938.5 65 4549.0 66 3402.0 67 2593.5 68 1957.5 69 1444.0 70 1043.5 71 760.0 72 565.5 73 401.5 74 293.5 75 213.0 76 143.5 77 100.0 78 63.0 79 43.5 80 39.5 81 29.0 82 17.0 83 11.0 84 5.5 85 6.0 86 6.5 87 3.5 88 1.0 89 1.5 90 3.0 91 3.0 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16455935833003754 2 0.0 3 4.521689640869322E-5 4 0.02301540027202485 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 9.796994221883531E-5 12-13 7.5361494014488695E-6 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 6.782534461303983E-5 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0011982477548303702 38-39 5.049220098970743E-4 40-41 3.2405442426230145E-4 42-43 5.652112051086652E-4 44-45 3.2405442426230145E-4 46-47 0.00360227941389256 48-49 0.004077056826183839 50-51 0.00474023797351134 52-53 0.004423719698650486 54-55 0.0063379016466185 56-57 0.009849747267693672 58-59 0.017385896669142545 60-61 0.004898497110941765 62-63 0.0030295320593824457 64-65 0.010761621345268986 66-67 0.02315105096125093 68-69 0.025592763367320363 70-71 0.025879137044575416 72-73 0.023203804007061072 74-75 0.025050160610416043 76-77 0.02503508831161315 78-79 0.016217793511917968 80-81 0.0035043094716737245 82-83 0.0012133200536332681 84-85 0.005712401246298243 86-87 0.015230557940328166 88-89 0.0170090891990701 90-91 0.02367858141935235 92-93 0.024228720325658114 94-95 0.02538175118407979 96-97 0.026557390490705816 98-99 0.032752105298696786 100-101 0.023821768257979877 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6634688.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.648764316548288 #Duplication Level Percentage of deduplicated Percentage of total 1 84.62988818576717 26.784313853271797 2 6.5703430413652315 4.158864767900825 3 2.5204712529674516 2.393094019554061 4 1.200839796804964 1.5202038284404817 5 0.7770002782179702 1.2295549339606493 6 0.5436382449497146 1.0323287212725276 7 0.4145028286660417 0.9182951633095904 8 0.2969628397992714 0.751880554206402 9 0.319192733396954 0.9091850031751525 >10 2.151783147139586 13.519637479855923 >50 0.2621920773900362 5.834465919019001 >100 0.24489737632823255 17.21464595761805 >500 0.0482194073745689 10.527722406788865 >1k 0.01978109167911505 10.048134423187385 >5k 2.3975801279698286E-4 0.4426215520417263 >10k+ 4.794014080111836E-5 2.7150514163974977 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT 178946 2.697127581583339 TruSeq Adapter, Index 7 (97% over 38bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0019895434419825017 0.0 0.0 2 0.0022156279240259677 0.0 0.0 3 0.002486929302478127 0.0 0.0 4 0.002607507692901309 0.0 0.0 5 0.0029692428641708546 0.0 0.0 6 0.003315905736637503 0.0 0.0 7 0.003903725389950515 0.0 0.0 8 0.004340822055234549 0.0 0.0 9 0.004943714007350459 0.0 0.0 10-11 0.007566293999054665 0.0 0.0 12-13 0.01019641014016032 0.0 0.0 14-15 0.012864207028273221 0.0 0.0 16-17 0.015253166388532514 0.0 0.0 18-19 0.01859168057337436 0.0 1.5072298802897739E-5 20-21 0.02235221912469735 0.0 1.5072298802897739E-5 22-23 0.025924353940984114 0.0 1.5072298802897739E-5 24-25 0.030121989157591133 0.0 1.5072298802897739E-5 26-27 0.034372377420008295 0.0 1.5072298802897739E-5 28-29 0.03884885016446893 0.0 1.5072298802897739E-5 30-31 0.06631811473275005 0.0 1.5072298802897739E-5 32-33 0.07309311304465259 0.0 1.5072298802897739E-5 34-35 0.07845885141848419 0.0 1.5072298802897739E-5 36-37 0.08455559628425632 0.0 3.0144597605795478E-5 38-39 0.09155667907820232 0.0 3.0144597605795478E-5 40-41 0.09980122652338738 0.0 3.0144597605795478E-5 42-43 0.1090405456895637 0.0 3.0144597605795478E-5 44-45 0.11953086565638052 0.0 3.0144597605795478E-5 46-47 0.13117421648161903 0.0 4.521689640869322E-5 48-49 0.14419668264732266 0.0 5.2753045810142085E-5 50-51 0.15724175726123069 0.0 6.0289195211590956E-5 52-53 0.17226883916771973 0.0 6.0289195211590956E-5 54-55 0.18812489750836814 0.0 6.0289195211590956E-5 56-57 0.20612875842842948 0.0 6.0289195211590956E-5 58-59 0.22649143411114434 0.0 6.0289195211590956E-5 60-61 0.24870046639721416 0.0 6.0289195211590956E-5 62-63 0.27337381953755774 0.0 6.0289195211590956E-5 64-65 0.29985584853424907 0.0 6.0289195211590956E-5 66-67 0.3297743616580011 0.0 6.0289195211590956E-5 68-69 0.3625566115543037 0.0 6.0289195211590956E-5 70-71 0.39885070707168147 0.0 6.0289195211590956E-5 72-73 0.4392520040128488 0.0 6.0289195211590956E-5 74-75 0.4836474601367841 0.0 6.0289195211590956E-5 76-77 0.5331524255549017 0.0 6.0289195211590956E-5 78-79 0.5889952926196378 0.0 6.0289195211590956E-5 80-81 0.6530224179343476 0.0 6.0289195211590956E-5 82-83 0.7238546861585654 0.0 6.0289195211590956E-5 84-85 0.8025094774614873 0.0 7.53614940144887E-5 86-87 0.889069689486529 0.0 7.53614940144887E-5 88-89 0.9834373522914717 0.0 7.53614940144887E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAAGA 28755 0.0 67.77761 4 TCGGAAG 28930 0.0 66.85863 3 GAAGAGC 29140 0.0 66.73915 6 GATCGGA 29565 0.0 65.64246 1 ATCGGAA 30020 0.0 64.44688 2 GGAAGAG 32045 0.0 60.94094 5 TCGCCGG 255 0.0 46.569115 2 TTGTGTT 61430 0.0 46.26939 1 GCCGTCT 21805 0.0 44.64474 50-51 ATGCCGT 21850 0.0 44.376045 48-49 TCGTATG 21975 0.0 44.349228 44-45 TATGCCG 21775 0.0 44.321674 48-49 TGCCGTC 21855 0.0 44.292667 50-51 CGTATGC 21865 0.0 44.224964 46-47 TCTCGTA 21780 0.0 44.015617 42-43 CGTCTTC 22125 0.0 43.98038 52-53 CATCTCG 21740 0.0 43.724064 40-41 CTCGTAT 21905 0.0 43.612835 44-45 ATCTCGT 21850 0.0 43.396408 42-43 GTATGCC 22470 0.0 43.21388 46-47 >>END_MODULE