FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n01_52260024-1.34100000035820.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n01_52260024-1.34100000035820.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6634688
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT1789462.697127581583339TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA287550.067.777614
TCGGAAG289300.066.858633
GAAGAGC291400.066.739156
GATCGGA295650.065.642461
ATCGGAA300200.064.446882
GGAAGAG320450.060.940945
TCGCCGG2550.046.5691152
TTGTGTT614300.046.269391
GCCGTCT218050.044.6447450-51
ATGCCGT218500.044.37604548-49
TCGTATG219750.044.34922844-45
TATGCCG217750.044.32167448-49
TGCCGTC218550.044.29266750-51
CGTATGC218650.044.22496446-47
TCTCGTA217800.044.01561742-43
CGTCTTC221250.043.9803852-53
CATCTCG217400.043.72406440-41
CTCGTAT219050.043.61283544-45
ATCTCGT218500.043.39640842-43
GTATGCC224700.043.2138846-47
Produced by FastQC (version 0.11.2)