##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n01_52150223-1.3410000003573e.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3230161 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67841510067145 33.0 33.0 33.0 33.0 33.0 2 32.72556197663212 33.0 33.0 33.0 33.0 33.0 3 32.570397265027964 33.0 33.0 33.0 33.0 33.0 4 36.613300080088884 37.0 37.0 37.0 37.0 37.0 5 36.67690279215184 37.0 37.0 37.0 37.0 37.0 6 36.55718182468304 37.0 37.0 37.0 37.0 37.0 7 36.47697003338224 37.0 37.0 37.0 37.0 37.0 8 36.4260657595705 37.0 37.0 37.0 37.0 37.0 9 36.45130567795228 37.0 37.0 37.0 37.0 37.0 10-11 36.388708643315304 37.0 37.0 37.0 37.0 37.0 12-13 36.3622368977893 37.0 37.0 37.0 37.0 37.0 14-15 38.18356484398146 40.0 37.0 40.0 37.0 40.0 16-17 38.190292217632496 40.0 37.0 40.0 37.0 40.0 18-19 38.18318328405302 40.0 37.0 40.0 37.0 40.0 20-21 38.13305869274008 40.0 37.0 40.0 37.0 40.0 22-23 37.97975286680757 40.0 37.0 40.0 33.0 40.0 24-25 37.97508405927754 40.0 37.0 40.0 33.0 40.0 26-27 37.91521057309527 40.0 37.0 40.0 33.0 40.0 28-29 37.73154186432193 40.0 37.0 40.0 33.0 40.0 30-31 37.680333890477904 40.0 37.0 40.0 33.0 40.0 32-33 37.568958637046265 40.0 37.0 40.0 33.0 40.0 34-35 37.5077047243156 40.0 37.0 40.0 33.0 40.0 36-37 37.42400425241962 40.0 37.0 40.0 33.0 40.0 38-39 37.232729266435946 40.0 37.0 40.0 33.0 40.0 40-41 37.12957357233896 40.0 37.0 40.0 33.0 40.0 42-43 36.95631905035074 38.5 37.0 40.0 33.0 40.0 44-45 36.791305603652575 37.0 37.0 40.0 33.0 40.0 46-47 36.806029792323045 37.0 37.0 40.0 33.0 40.0 48-49 36.55283544690187 37.0 37.0 40.0 33.0 40.0 50-51 36.45266660082888 37.0 37.0 40.0 33.0 40.0 52-53 36.37570309962878 37.0 37.0 40.0 33.0 40.0 54-55 36.11865074217663 37.0 37.0 40.0 30.0 40.0 56-57 35.951906422001876 37.0 37.0 40.0 27.0 40.0 58-59 35.797021727399965 37.0 37.0 40.0 27.0 40.0 60-61 35.51069234629482 37.0 35.0 40.0 27.0 40.0 62-63 35.30280750711807 37.0 33.0 40.0 27.0 40.0 64-65 35.07022451822061 37.0 33.0 40.0 27.0 40.0 66-67 34.600832590078326 37.0 33.0 40.0 27.0 40.0 68-69 34.42797928648139 37.0 33.0 37.0 27.0 40.0 70-71 34.135977897076955 37.0 33.0 37.0 27.0 40.0 72-73 33.596816381598316 37.0 33.0 37.0 24.5 40.0 74-75 33.0159927012926 37.0 33.0 37.0 22.0 40.0 76-77 30.500388989898646 33.0 30.0 35.0 22.0 37.0 78-79 32.57265674992671 37.0 33.0 37.0 22.0 37.0 80-81 32.804586520609966 37.0 33.0 37.0 22.0 37.0 82-83 32.65887985149966 37.0 33.0 37.0 22.0 37.0 84-85 32.45275777275498 37.0 33.0 37.0 22.0 37.0 86-87 32.260041217759735 37.0 33.0 37.0 22.0 37.0 88-89 32.09743616494657 37.0 33.0 37.0 22.0 37.0 90-91 31.932289907530922 33.0 33.0 37.0 22.0 37.0 92-93 31.75451455825267 33.0 33.0 37.0 15.0 37.0 94-95 31.616177490843334 33.0 33.0 37.0 15.0 37.0 96-97 31.468441820701816 33.0 33.0 37.0 15.0 37.0 98-99 31.290618486199293 33.0 33.0 37.0 15.0 37.0 100-101 29.65269718753957 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 0.14541266884876336 2306 2 0.12927465658373904 2306 3 0.3061626080639712 2306 4 0.20419646896527155 2306 5 0.14000369462221585 2306 6 0.15076190111236087 2306 7 0.17882585692822062 2306 8 0.1253711841512981 2306 9 0.20143143848277134 2306 10-11 0.23962329341664912 2306 12-13 0.13565141973930395 2306 14-15 0.33798314628687365 2306 16-17 0.2666655837033076 2306 18-19 0.38473446740427875 2306 20-21 0.2530492764576948 2306 22-23 0.36903742310211385 2306 24-25 0.36157710388453523 2306 26-27 0.29210963829351044 2306 28-29 0.36088497741683767 2306 30-31 0.2761904966895017 2306 32-33 0.3300830801572019 2306 34-35 0.2816461945693831 2306 36-37 0.3305187280048827 2306 38-39 0.3906333350568616 2306 40-41 0.4277069422768207 2306 42-43 0.5052265507202307 2306 44-45 0.2775458952302259 2306 46-47 0.2258125130152564 2306 48-49 0.12913115490420068 2306 50-51 0.18615241655692216 2306 52-53 0.1804925859718267 2306 54-55 0.07443616975997003 2306 56-57 0.13248098437045286 2306 58-59 0.24885946320659258 2306 60-61 0.1448318790384704 2306 62-63 0.17713449867455466 2306 64-65 0.05876494294871293 2306 66-67 0.040706857344197545 2306 68-69 0.2489331938160788 2306 70-71 0.38001373746840983 2306 72-73 0.24483228729335593 2306 74-75 0.23695216113162587 2306 76-77 0.05820573955801933 2306 78-79 0.2862828943907516 2306 80-81 0.32559333899352083 2306 82-83 0.401215521343083 2306 84-85 0.4602268133506868 2306 86-87 0.39494434814814383 2306 88-89 0.4336897156367243 2306 90-91 0.4892703709585362 2306 92-93 0.5395554173320534 2306 94-95 0.36110973295062365 2306 96-97 0.5221119729067283 2306 98-99 0.1983979448612594 2306 100-101 0.3836385930765225 2307 1 0.1625570843387365 2307 2 0.12686138292933435 2307 3 0.3121505568462908 2307 4 0.22922265785425822 2307 5 0.12428862973437305 2307 6 0.15468244272455678 2307 7 0.18054826336246066 2307 8 0.15644852145553045 2307 9 0.16657987104530037 2307 10-11 0.18189735472895308 2307 12-13 0.1285756580971551 2307 14-15 0.2720869235471284 2307 16-17 0.1989636262461829 2307 18-19 0.2643781253644022 2307 20-21 0.2910266284796563 2307 22-23 0.30886834244034134 2307 24-25 0.24162723071667358 2307 26-27 0.31215474378495855 2307 28-29 0.38856295364337967 2307 30-31 0.4055716164484764 2307 32-33 0.426796699245223 2307 34-35 0.37665277425720234 2307 36-37 0.3873661485075317 2307 38-39 0.4102442677276059 2307 40-41 0.43406824505500197 2307 42-43 0.511698669293132 2307 44-45 0.43909086653066254 2307 46-47 0.30910876345895133 2307 48-49 0.3033270910812291 2307 50-51 0.191603601333604 2307 52-53 0.10601658018862281 2307 54-55 0.2624390613596219 2307 56-57 0.10074416322541424 2307 58-59 0.20065221169803493 2307 60-61 0.04271837076624507 2307 62-63 0.10799539887972998 2307 64-65 -0.03360348493878007 2307 66-67 -0.0026584798215125716 2307 68-69 0.011435682842716233 2307 70-71 0.1399620522891567 2307 72-73 0.05930348817295794 2307 74-75 0.13056055715956916 2307 76-77 -0.08408628603350365 2307 78-79 0.24195181488907735 2307 80-81 0.2747189331133626 2307 82-83 0.2797077339362559 2307 84-85 0.49477688390917507 2307 86-87 0.43382121275375596 2307 88-89 0.28028689156545994 2307 90-91 0.15499149608514529 2307 92-93 0.3753883105766249 2307 94-95 0.3880990063279981 2307 96-97 0.3889856255857893 2307 98-99 0.26650182601132144 2307 100-101 0.2115559487346168 2201 1 0.08605201838518894 2201 2 0.06821013250263519 2201 3 0.28206228091020336 2201 4 0.20657679461487533 2201 5 0.1640254714473599 2201 6 0.09346653934213833 2201 7 0.19319973142317792 2201 8 0.1513717607441336 2201 9 0.20379379783381069 2201 10-11 0.1452584438530593 2201 12-13 0.18026299254250233 2201 14-15 0.2865385305358572 2201 16-17 0.33248992344184103 2201 18-19 0.23068808092830295 2201 20-21 0.22057059576896876 2201 22-23 0.32510480925637353 2201 24-25 0.26212507599080936 2201 26-27 0.24425702879310052 2201 28-29 0.3020046120742421 2201 30-31 0.3387980333428473 2201 32-33 0.3608267595715873 2201 34-35 0.42342452387074303 2201 36-37 0.3221838278431832 2201 38-39 0.2927450018697684 2201 40-41 0.28894716004506904 2201 42-43 0.39742646130655856 2201 44-45 0.2894494036722861 2201 46-47 0.3694835709795541 2201 48-49 0.14551224098784132 2201 50-51 0.2017910349067833 2201 52-53 0.1991482890181615 2201 54-55 0.12830644472790453 2201 56-57 0.27413605650722417 2201 58-59 0.33305810361739674 2201 60-61 0.3067429082566875 2201 62-63 0.15190939766635125 2201 64-65 0.22000014686619807 2201 66-67 0.2696016792898206 2201 68-69 0.36023127977855296 2201 70-71 0.24855621105167813 2201 72-73 0.18665386105755033 2201 74-75 0.020937295064115347 2201 76-77 0.10493649995026644 2201 78-79 0.3744804986057275 2201 80-81 0.36642446832162534 2201 82-83 0.44529144775646046 2201 84-85 0.5546633191980845 2201 86-87 0.530643383399692 2201 88-89 0.5724967116298245 2201 90-91 0.4769329555150392 2201 92-93 0.413448923141857 2201 94-95 0.4543681927792349 2201 96-97 0.3978566342002203 2201 98-99 0.25522827197324105 2201 100-101 0.23370356422598704 1101 1 0.12494783775880336 1101 2 0.12949572664626885 1101 3 0.28697789777002924 1101 4 0.17975905056306374 1101 5 0.14351662304974155 1101 6 0.13254405950697645 1101 7 0.1471410944951117 1101 8 0.22443810164211442 1101 9 0.07985374904033193 1101 10-11 0.1506509355167296 1101 12-13 0.15600988693125117 1101 14-15 0.21260659723225928 1101 16-17 0.16468888365367462 1101 18-19 0.18774331437791147 1101 20-21 0.23806594013505133 1101 22-23 0.21737168103665994 1101 24-25 0.3075475806033481 1101 26-27 0.23159650548046073 1101 28-29 0.2436052236805395 1101 30-31 0.2562981536388236 1101 32-33 0.3218530171313816 1101 34-35 0.43948062560595247 1101 36-37 0.3637338792293221 1101 38-39 0.3814476654022698 1101 40-41 0.4754355516627058 1101 42-43 0.46398676478278844 1101 44-45 0.2965675142138835 1101 46-47 0.5862472285231206 1101 48-49 0.497721270152617 1101 50-51 0.2992009727191416 1101 52-53 0.20736719753504218 1101 54-55 0.42497966842312707 1101 56-57 0.3331164565218856 1101 58-59 0.25734417391765163 1101 60-61 0.47225311761478395 1101 62-63 0.4751100391491434 1101 64-65 0.2839590049234104 1101 66-67 0.1382519368668511 1101 68-69 0.14769144744600027 1101 70-71 0.26533426820340367 1101 72-73 0.3969107629944162 1101 74-75 0.25721385803205976 1101 76-77 -0.028699404700624598 1101 78-79 0.31621828353663517 1101 80-81 0.4792468346117431 1101 82-83 0.5175765829799133 1101 84-85 0.5109075422979288 1101 86-87 0.5399702717413319 1101 88-89 0.743327953249505 1101 90-91 0.7422101856767966 1101 92-93 0.5470350934852704 1101 94-95 0.5804927806308484 1101 96-97 0.5941751822600807 1101 98-99 0.3445509086599934 1101 100-101 0.44397716396284537 2304 1 0.10815706984413964 2304 2 0.056895284168817284 2304 3 0.28200398699813434 2304 4 0.14465845855630732 2304 5 0.06605526546582752 2304 6 0.09953639293697591 2304 7 0.1271133806512239 2304 8 0.14460238020438965 2304 9 0.11704225523116207 2304 10-11 0.03236936842365168 2304 12-13 0.11586789389463092 2304 14-15 0.2715322202198749 2304 16-17 0.2287937979467074 2304 18-19 0.2727675413598547 2304 20-21 0.24315219995913395 2304 22-23 0.27808931181984065 2304 24-25 0.2216188225113953 2304 26-27 0.16751163619817788 2304 28-29 0.19369306609055315 2304 30-31 0.17163586842353595 2304 32-33 0.2836397905926944 2304 34-35 0.12925668403909896 2304 36-37 0.2066205191973225 2304 38-39 0.24326002858294515 2304 40-41 0.2582328264465019 2304 42-43 0.3187923459603965 2304 44-45 0.2646963512573208 2304 46-47 0.22934354900274911 2304 48-49 0.15577634155650344 2304 50-51 0.07144293281081815 2304 52-53 0.23358978962004073 2304 54-55 0.2930355674919696 2304 56-57 0.2682039222796675 2304 58-59 0.09171839293008333 2304 60-61 0.23243638720821735 2304 62-63 0.28095463082742356 2304 64-65 0.1664477158643365 2304 66-67 0.11551119547949185 2304 68-69 0.1729001499550904 2304 70-71 0.14106005116313725 2304 72-73 0.026821426907808643 2304 74-75 0.015567697088080479 2304 76-77 -0.09505679624983898 2304 78-79 0.16561195429215303 2304 80-81 0.25621618417378045 2304 82-83 0.301819200030657 2304 84-85 0.3194925617561353 2304 86-87 0.3197192500115662 2304 88-89 0.3773731431097005 2304 90-91 0.3776715393810264 2304 92-93 0.29670297675477286 2304 94-95 0.3656929510781026 2304 96-97 0.2425827839747683 2304 98-99 0.3182578357307513 2304 100-101 0.16358036004281828 2202 1 0.10534340217206051 2202 2 0.06339423123185384 2202 3 0.30736868001240225 2202 4 0.22149951426109027 2202 5 0.15034816368525128 2202 6 0.13162429274873233 2202 7 0.11457025858889835 2202 8 0.13838521674822601 2202 9 0.08051376257338205 2202 10-11 0.13465577561597541 2202 12-13 0.12299626883689996 2202 14-15 0.08952085216880334 2202 16-17 0.0882248368278411 2202 18-19 0.057211002062707905 2202 20-21 0.09542151672558674 2202 22-23 0.1322733815421131 2202 24-25 0.13415043979923524 2202 26-27 0.15413504324543226 2202 28-29 0.19453112221717817 2202 30-31 0.1217093155707687 2202 32-33 0.14250142650492847 2202 34-35 0.06048418511871745 2202 36-37 0.048719156798597396 2202 38-39 0.15238106381256955 2202 40-41 0.11849379308098662 2202 42-43 0.17872380084750716 2202 44-45 0.16754811807388137 2202 46-47 0.21576687301554642 2202 48-49 0.16406332232018173 2202 50-51 0.20307255910574185 2202 52-53 0.22490285120726838 2202 54-55 0.1904825913401993 2202 56-57 0.21905644274232827 2202 58-59 0.1467264295986368 2202 60-61 0.27669329549181754 2202 62-63 0.2327833897623819 2202 64-65 0.1419998616715077 2202 66-67 0.1919310347127805 2202 68-69 0.05578779832917036 2202 70-71 0.11014743075900668 2202 72-73 -0.014542197329582507 2202 74-75 -0.15917148633970157 2202 76-77 -0.3028803346849607 2202 78-79 0.06016215095710464 2202 80-81 -0.0052360685110883765 2202 82-83 0.0017525890537655187 2202 84-85 0.08059612806948735 2202 86-87 0.2525086492789299 2202 88-89 0.10955303943334371 2202 90-91 0.026885546527324777 2202 92-93 0.12322952693975964 2202 94-95 0.18675973546028501 2202 96-97 0.05885737126182633 2202 98-99 0.039364595035863204 2202 100-101 0.09931806619086814 1102 1 0.09804358494659482 1102 2 0.09774792282028955 1102 3 0.29631183064562094 1102 4 0.19757986700849983 1102 5 0.07856875415652098 1102 6 0.07506067732166599 1102 7 0.11244825578827289 1102 8 0.08977798210889176 1102 9 0.08951054408284875 1102 10-11 0.15512740090584742 1102 12-13 0.14039653231292704 1102 14-15 0.08818699145810172 1102 16-17 0.12800660785887175 1102 18-19 0.09998093474766989 1102 20-21 0.07677232049761074 1102 22-23 0.15299500603453708 1102 24-25 0.05096941857973292 1102 26-27 0.16211867009191394 1102 28-29 0.2197406377636426 1102 30-31 0.12654529163889805 1102 32-33 0.22612330224588817 1102 34-35 0.050279406399248217 1102 36-37 0.10867616916325318 1102 38-39 0.09825656872855149 1102 40-41 0.10309259287216577 1102 42-43 0.1611103535751468 1102 44-45 0.2508200480943046 1102 46-47 0.2763853665238116 1102 48-49 0.18082416731483164 1102 50-51 0.15113944459148598 1102 52-53 0.09386738401577333 1102 54-55 0.32534978474628673 1102 56-57 0.30796073119223166 1102 58-59 0.13950489495174878 1102 60-61 0.1505471003175316 1102 62-63 0.20833450443186763 1102 64-65 -0.032838363193270936 1102 66-67 -0.021146568442127034 1102 68-69 -0.13075227650313082 1102 70-71 -0.1978176953490447 1102 72-73 -0.2459443034366373 1102 74-75 -0.1846514688596983 1102 76-77 -0.15659039727912116 1102 78-79 -0.1362786099974045 1102 80-81 -0.08181849302222588 1102 82-83 0.0041159520627118695 1102 84-85 -0.034401245676264125 1102 86-87 0.06382836330429598 1102 88-89 0.04942231683065046 1102 90-91 0.10314101833337475 1102 92-93 0.08762743422979469 1102 94-95 -0.12284281232531313 1102 96-97 -0.1520268577092665 1102 98-99 -0.24911561183216335 1102 100-101 -0.3279132929860822 2305 1 0.10492112007653986 2305 2 0.08011821750098846 2305 3 0.2521087988421158 2305 4 0.1005657677835643 2305 5 0.04483140577796263 2305 6 0.08266102990651092 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-0.2044205944323778 1213 82-83 -0.1739219600666715 1213 84-85 -0.16642284004299768 1213 86-87 -0.1655151090411593 1213 88-89 -0.12279352719522052 1213 90-91 -0.12212341631461499 1213 92-93 -0.0787670377178209 1213 94-95 0.03091224393184433 1213 96-97 0.03070449925424512 1213 98-99 0.15242678013555633 1213 100-101 0.2890054325110789 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 3.0 9 10.0 10 13.0 11 26.0 12 42.0 13 129.0 14 374.0 15 842.0 16 1708.0 17 2879.0 18 4262.0 19 5820.0 20 7933.0 21 10585.0 22 13490.0 23 17310.0 24 21869.0 25 28128.0 26 36263.0 27 49693.0 28 72152.0 29 88015.0 30 75239.0 31 77916.0 32 101965.0 33 141390.0 34 208877.0 35 335007.0 36 580031.0 37 932181.0 38 415039.0 39 970.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.98494851051031 26.373006545878514 19.545571531785185 16.096473411825993 2 29.530107013241757 27.718804109145022 28.445795735878182 14.30529314173504 3 34.89378235133863 21.2335921440424 27.31536518314882 16.557260321470142 4 29.42866246554761 21.670980601382833 28.240258346836633 20.660098586232916 5 34.36661516252595 19.90665480760866 28.783766505756216 16.94296352410917 6 31.046842556764197 20.739492551609658 31.0176180072758 17.196046884350345 7 31.101143255707687 24.865912256385982 25.126920918183338 18.906023569722997 8 28.61123640586336 30.232734529331513 23.064020647887208 18.092008416917917 9 31.82637026451623 24.501441259429484 24.787742778146352 18.884445697907935 10-11 26.380061269496156 29.995274221318446 27.927943407058887 15.696721102126507 12-13 24.016929806285198 29.350286255081404 27.655927986252077 18.97685595238132 14-15 23.309983620011508 29.63832143351369 27.926487255588807 19.125207690885997 16-17 24.91908298069353 27.624025551667547 28.13305590650125 19.323835561137663 18-19 22.05724420547459 27.683991602895336 30.585812905301008 19.67295128632907 20-21 24.000537434511777 27.712364801630628 30.51180730619929 17.7752904576583 22-23 21.592329298756315 32.54165968817034 27.943576187069315 17.92243482600403 24-25 21.31489730697634 27.923112810785593 30.481979071631415 20.280010810606655 26-27 21.301913635560833 27.886540003197986 28.177686526722123 22.63385983451906 28-29 23.30529135271134 30.35598579264104 28.073743783970873 18.264979070676752 30-31 20.856158562994228 28.159014364918654 30.447321356427743 20.537505715659375 32-33 20.81312665220093 30.471824779012564 28.117948300409797 20.597100268376717 34-35 20.720700918622942 28.20549811603818 30.450958946009195 20.62284201932969 36-37 22.975250516388204 28.12221373270657 28.174332410356495 20.728203340548735 38-39 22.870747303175417 30.48671397212785 28.135671455878946 18.50686726881779 40-41 22.72991895588895 30.393716461550056 28.14366928888386 18.73269529367713 42-43 20.50944763943296 30.33200826402402 30.330119804200773 18.82842429234225 44-45 20.31940926591501 28.10943329111136 30.52015687813927 21.051000564834364 46-47 22.598789247747252 28.084557614754907 28.10542411312738 21.21122902437046 48-49 20.26102704030605 30.43912498527499 30.26329171218327 19.036556262235692 50-51 22.533633640606496 28.11091245423797 30.196143992693553 19.15930991246198 52-53 20.341355645592476 28.19570264452407 27.932764154421953 23.530177555461506 54-55 22.341187840202306 28.096068339921885 30.407094923283378 19.15564889659243 56-57 20.12663720508945 28.14778303618133 30.292754492343544 21.432825266385674 58-59 20.063072386943055 28.39604319080636 30.278585190684048 21.262299231566537 60-61 22.21888031068529 28.24105477952001 30.315607855181128 19.224457054613577 62-63 19.997442784232135 28.36689111446466 30.21118237019537 21.42448373110783 64-65 22.118403500371613 28.317231085179912 30.28814068610764 19.27622472834084 66-67 20.039227028128394 32.491578946716515 27.90228295853789 19.566911066617205 68-69 19.972774525725704 32.70997360596793 27.885131537407855 19.432120330898513 70-71 19.916081469100718 32.6588936224239 27.950135051547193 19.474889856928197 72-73 19.81043415684244 32.57665744316648 27.909582861630156 19.703325538360925 74-75 19.78638877564876 32.32678556802771 28.07549394673236 19.81133170959117 76-77 19.89365390031435 31.919761847659363 28.061537861276914 20.12504639074938 78-79 19.847605966459565 31.769796393050154 28.189505748913042 20.19309189157724 80-81 19.873339755301643 31.44806820062237 28.22998406994796 20.448607974128024 82-83 19.916972304804116 31.415359210334454 28.035832646827817 20.631835838033613 84-85 19.9314208509282 31.139069913673122 28.29372098772427 20.635788247674412 86-87 20.04465121290696 30.88336856106892 28.289551396345082 20.782428829679045 88-89 20.112734433140933 31.00652072508097 28.196953485783418 20.683791355994682 90-91 20.16131816660987 30.735390179180648 28.202701775828253 20.900589878381226 92-93 20.101526787997994 30.837116808912135 28.062602457303136 20.99875394578673 94-95 20.062988210109545 30.708695553216923 28.22821544191226 21.000100794761273 96-97 20.15708699131869 30.62244110050697 28.09295593602749 21.12751597214685 98-99 20.126531258792163 30.639173623103893 28.09534036515315 21.1389547529508 100-101 20.262968882547142 30.55005529676083 27.92188378406038 21.265092036631646 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 14.0 1 7.5 2 5.0 3 14.0 4 13.0 5 6.0 6 8.5 7 10.5 8 12.5 9 17.5 10 17.5 11 20.0 12 32.0 13 53.0 14 79.5 15 120.5 16 203.5 17 352.0 18 593.5 19 1042.0 20 1843.5 21 2987.0 22 4737.5 23 7172.0 24 10273.0 25 14439.0 26 19514.0 27 25560.5 28 32601.5 29 40257.0 30 48626.0 31 57799.0 32 67835.5 33 78883.5 34 90940.0 35 103874.5 36 117769.5 37 132071.0 38 145104.5 39 154676.5 40 161630.5 41 164767.0 42 164554.5 43 162702.0 44 157637.5 45 149958.5 46 141167.5 47 131067.0 48 119199.5 49 107038.5 50 96054.0 51 86128.5 52 75729.5 53 65226.0 54 55455.5 55 46390.5 56 38457.5 57 31868.0 58 26059.0 59 20940.5 60 16665.5 61 13012.0 62 9873.0 63 7448.5 64 5631.0 65 4235.5 66 3115.0 67 2284.5 68 1695.0 69 1200.0 70 847.0 71 628.0 72 474.0 73 351.5 74 258.0 75 194.5 76 143.5 77 112.5 78 92.5 79 79.5 80 56.0 81 37.0 82 32.0 83 23.0 84 17.0 85 11.0 86 11.0 87 7.5 88 3.5 89 2.0 90 1.5 91 1.5 92 1.5 93 1.5 94 1.0 95 0.5 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16194239234514937 2 0.0 3 3.095820920381368E-5 4 0.021794579279484833 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.7027015062097525E-4 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.547910460190684E-5 28-29 1.2383283681525472E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0010216209037258515 38-39 6.036850794743667E-4 40-41 2.012283598247889E-4 42-43 4.79852242659112E-4 44-45 3.2506119664004363E-4 46-47 0.0031886955479928088 48-49 0.003482798535429039 50-51 0.00421031645171866 52-53 0.003962650778088151 54-55 0.005866580644122692 56-57 0.009426774702561265 58-59 0.016670995656253667 60-61 0.004334149288533915 62-63 0.00264692688692607 64-65 0.010293604560268049 66-67 0.023373447948879328 68-69 0.02577270916217489 70-71 0.025958458417397773 72-73 0.02256853450958017 74-75 0.024441506166410897 76-77 0.024751088258449034 78-79 0.015695812066333536 80-81 0.003173216443390902 82-83 0.001052579112929665 84-85 0.005278374669250232 86-87 0.014287213547560013 88-89 0.016578121028642225 90-91 0.023729467354723185 92-93 0.02374494645932509 94-95 0.025586959906952006 96-97 0.02685624648430837 98-99 0.03335747041710924 100-101 0.023760425563927 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3230161.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.42774441272852 #Duplication Level Percentage of deduplicated Percentage of total 1 97.75879939576178 87.42348926459384 2 1.5210742912759878 2.720524859060024 3 0.26390313591378206 0.7080078656464577 4 0.09741162529326795 0.3484520771821939 5 0.06726691892412727 0.30077644164892925 6 0.07184371271138772 0.38548927068092853 7 0.04817151176038977 0.3015508749178018 8 0.02194621855286829 0.1570080658857429 9 0.016527771387620356 0.13302371838277086 >10 0.11657297271033039 2.163050185287492 >50 0.012415113310921511 0.7153307069437027 >100 0.0036840423316568086 0.5202754463671979 >500 2.7820953746912896E-4 0.1971398057864296 >1k 7.061461108943671E-5 0.1270585266296558 >5k 0.0 0.0 >10k+ 3.446591728798298E-5 3.7988228909868296 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT 123250 3.8155992843700357 TruSeq Adapter, Index 6 (97% over 36bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0022289910626745848 0.0 0.0 2 0.0024456985271012806 0.0 0.0 3 0.002817197037547045 0.0 3.095820920381368E-5 4 0.0031577373387889954 0.0 3.095820920381368E-5 5 0.0038697761504767098 0.0 3.095820920381368E-5 6 0.0044579821253491695 0.0 3.095820920381368E-5 7 0.005417686610667394 0.0 3.095820920381368E-5 8 0.006067809003947482 0.0 3.095820920381368E-5 9 0.007213262744488588 0.0 6.191641840762736E-5 10-11 0.01047935381549093 0.0 6.191641840762736E-5 12-13 0.013048885179407466 0.0 6.191641840762736E-5 14-15 0.016051831472177393 0.0 6.191641840762736E-5 16-17 0.019132173287956855 0.0 6.191641840762736E-5 18-19 0.022723325555599243 0.0 6.191641840762736E-5 20-21 0.026562143496872137 0.0 1.0835373221334788E-4 22-23 0.032072704735150975 0.0 1.2383283681525472E-4 24-25 0.03718080925378023 0.0 1.3931194141716155E-4 26-27 0.041870977948158 0.0 1.547910460190684E-4 28-29 0.0486508257637932 0.0 1.8574925522288208E-4 30-31 0.0812652991600109 0.0 2.1670746442669576E-4 32-33 0.0894073081806139 0.0 2.1670746442669576E-4 34-35 0.09875668736016563 0.0 2.4766567363050944E-4 36-37 0.1083692113179498 0.0 2.4766567363050944E-4 38-39 0.11898787707485789 0.0 2.786238828343231E-4 40-41 0.1310306204551414 0.0 2.786238828343231E-4 42-43 0.1445748369818099 0.0 2.9410298743623E-4 44-45 0.15844411470511843 0.0 3.405403012419505E-4 46-47 0.17415540587605385 0.0 3.86977615047671E-4 48-49 0.19047038212646367 0.0 4.024567196495778E-4 50-51 0.20780697928059932 0.0 4.024567196495778E-4 52-53 0.22727969286979813 0.0 4.024567196495778E-4 54-55 0.25014233036681455 0.0 4.179358242514847E-4 56-57 0.270342561872303 0.0 4.334149288533915E-4 58-59 0.29510912923535393 0.0 4.334149288533915E-4 60-61 0.32129977422178024 0.0 4.334149288533915E-4 62-63 0.35024569982734605 0.0 4.334149288533915E-4 64-65 0.3813432209725769 0.0 4.334149288533915E-4 66-67 0.4153198555737624 0.0 4.334149288533915E-4 68-69 0.45061221406611 0.0 4.334149288533915E-4 70-71 0.48969695318592477 0.0 4.334149288533915E-4 72-73 0.5354531863891614 0.0 4.334149288533915E-4 74-75 0.5837634718517126 0.0 4.79852242659112E-4 76-77 0.6382344409458228 0.0 5.108104518629258E-4 78-79 0.6984017205334347 0.0 5.262895564648325E-4 80-81 0.7675468807901525 0.0 5.262895564648325E-4 82-83 0.8413667306366462 0.0 5.262895564648325E-4 84-85 0.9219199909849696 0.0 5.572477656686462E-4 86-87 1.0121786499186882 0.0 5.727268702705531E-4 88-89 1.1114306686261148 0.0 6.036850794743668E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 19045 0.0 72.1339 3 CGGAAGA 19215 0.0 71.742935 4 GATCGGA 19475 0.0 70.8271 1 ATCGGAA 19440 0.0 70.69266 2 GAAGAGC 20370 0.0 67.59147 6 AGAGCAC 20455 0.0 67.287384 8 GAGCACA 20435 0.0 67.144066 9 AAGAGCA 21955 0.0 62.62531 7 GGAAGAG 23710 0.0 58.350376 5 GCCGTCT 15175 0.0 44.82181 50-51 ATGCCGT 15185 0.0 44.541367 48-49 TCTCGTA 15040 0.0 44.40165 42-43 TCGTATG 15400 0.0 44.35063 44-45 CGTCTTC 15385 0.0 44.349625 52-53 TGCCGTC 15175 0.0 44.19581 50-51 AATCTCG 15115 0.0 43.960342 40-41 CGTATGC 15240 0.0 43.959194 46-47 TATGCCG 15220 0.0 43.892815 48-49 CTCGTAT 15095 0.0 43.689228 44-45 ATCTCGT 15100 0.0 43.643307 42-43 >>END_MODULE