FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n01_52150223-1.3410000003573e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n01_52150223-1.3410000003573e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3230161
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT1232503.8155992843700357TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG190450.072.13393
CGGAAGA192150.071.7429354
GATCGGA194750.070.82711
ATCGGAA194400.070.692662
GAAGAGC203700.067.591476
AGAGCAC204550.067.2873848
GAGCACA204350.067.1440669
AAGAGCA219550.062.625317
GGAAGAG237100.058.3503765
GCCGTCT151750.044.8218150-51
ATGCCGT151850.044.54136748-49
TCTCGTA150400.044.4016542-43
TCGTATG154000.044.3506344-45
CGTCTTC153850.044.34962552-53
TGCCGTC151750.044.1958150-51
AATCTCG151150.043.96034240-41
CGTATGC152400.043.95919446-47
TATGCCG152200.043.89281548-49
CTCGTAT150950.043.68922844-45
ATCTCGT151000.043.64330742-43
Produced by FastQC (version 0.11.2)