##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n01_50331580-1.341000000357a8.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3113244 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67399792627883 33.0 33.0 33.0 33.0 33.0 2 32.72492390573948 33.0 33.0 33.0 33.0 33.0 3 32.5615181463451 33.0 33.0 33.0 33.0 33.0 4 36.611356835506626 37.0 37.0 37.0 37.0 37.0 5 36.67745830394277 37.0 37.0 37.0 37.0 37.0 6 36.558679949274776 37.0 37.0 37.0 37.0 37.0 7 36.49098111166359 37.0 37.0 37.0 37.0 37.0 8 36.436200310672724 37.0 37.0 37.0 37.0 37.0 9 36.461535941288254 37.0 37.0 37.0 37.0 37.0 10-11 36.40221148743883 37.0 37.0 37.0 37.0 37.0 12-13 36.3720020017705 37.0 37.0 37.0 37.0 37.0 14-15 38.193263361304155 40.0 37.0 40.0 37.0 40.0 16-17 38.1818442434965 40.0 37.0 40.0 37.0 40.0 18-19 38.18876901392888 40.0 37.0 40.0 37.0 40.0 20-21 38.14130549998651 40.0 37.0 40.0 37.0 40.0 22-23 38.033803646614274 40.0 37.0 40.0 35.0 40.0 24-25 37.99055727723237 40.0 37.0 40.0 33.0 40.0 26-27 37.91262538368339 40.0 37.0 40.0 33.0 40.0 28-29 37.759133559720986 40.0 37.0 40.0 33.0 40.0 30-31 37.714203416115154 40.0 37.0 40.0 33.0 40.0 32-33 37.6076271246327 40.0 37.0 40.0 33.0 40.0 34-35 37.51984377067779 40.0 37.0 40.0 33.0 40.0 36-37 37.44788892229456 40.0 37.0 40.0 33.0 40.0 38-39 37.327230535094586 40.0 37.0 40.0 33.0 40.0 40-41 37.26133720967582 40.0 37.0 40.0 33.0 40.0 42-43 37.10006844950155 40.0 37.0 40.0 33.0 40.0 44-45 36.9947956215446 40.0 37.0 40.0 33.0 40.0 46-47 36.93263120397887 38.5 37.0 40.0 33.0 40.0 48-49 36.7279652670976 37.0 37.0 40.0 33.0 40.0 50-51 36.585653903131266 37.0 37.0 40.0 33.0 40.0 52-53 36.46886350700427 37.0 37.0 40.0 33.0 40.0 54-55 36.298438381315435 37.0 37.0 40.0 33.0 40.0 56-57 36.12826733143949 37.0 37.0 40.0 33.0 40.0 58-59 35.88753098054634 37.0 37.0 40.0 27.0 40.0 60-61 35.63730870436111 37.0 35.0 40.0 27.0 40.0 62-63 35.41570946575341 37.0 33.0 40.0 27.0 40.0 64-65 35.15368470958268 37.0 33.0 40.0 27.0 40.0 66-67 34.78167805029095 37.0 33.0 38.5 27.0 40.0 68-69 34.50424332304182 37.0 33.0 37.0 27.0 40.0 70-71 34.16883964122311 37.0 33.0 37.0 27.0 40.0 72-73 33.70512719208645 37.0 33.0 37.0 27.0 40.0 74-75 33.2303312878785 37.0 33.0 37.0 24.5 38.5 76-77 30.71270449730249 33.0 30.0 35.0 22.0 37.0 78-79 32.89649767252422 35.0 33.0 37.0 27.0 37.0 80-81 33.18604821851419 37.0 33.0 37.0 27.0 37.0 82-83 33.07091798779665 37.0 33.0 37.0 27.0 37.0 84-85 32.87612230201038 37.0 33.0 37.0 27.0 37.0 86-87 32.70022217982272 37.0 33.0 37.0 22.0 37.0 88-89 32.54784639430767 37.0 33.0 37.0 22.0 37.0 90-91 32.382976727811894 33.0 33.0 37.0 22.0 37.0 92-93 32.20277931957791 33.0 33.0 37.0 22.0 37.0 94-95 32.06158206680877 33.0 33.0 37.0 22.0 37.0 96-97 31.918479084838836 33.0 33.0 37.0 22.0 37.0 98-99 31.73787839950868 33.0 33.0 37.0 22.0 37.0 100-101 30.06447647534212 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 0.15495172332921214 2306 2 0.14538379399046164 2306 3 0.31426597421815217 2306 4 0.2173123448282368 2306 5 0.15829858684707432 2306 6 0.13317233585210886 2306 7 0.14799704507826306 2306 8 0.18774007676189086 2306 9 0.20418231253596275 2306 10-11 0.16257589214830404 2306 12-13 0.19195647818268924 2306 14-15 0.35486471614041903 2306 16-17 0.4030410138696894 2306 18-19 0.3967065917131478 2306 20-21 0.3992996416148742 2306 22-23 0.4578848580202646 2306 24-25 0.41150204935340184 2306 26-27 0.44221090877139346 2306 28-29 0.4355555285128716 2306 30-31 0.4771058002958526 2306 32-33 0.46254486300532704 2306 34-35 0.4217159899429106 2306 36-37 0.3517951017154104 2306 38-39 0.39773431596454856 2306 40-41 0.4738948249938346 2306 42-43 0.5021268940185379 2306 44-45 0.5517282858010688 2306 46-47 0.5801158149055894 2306 48-49 0.47192984020668405 2306 50-51 0.5050942939315703 2306 52-53 0.4991725588409892 2306 54-55 0.48376984740327345 2306 56-57 0.5590350419407955 2306 58-59 0.4840133247652645 2306 60-61 0.4495548953480153 2306 62-63 0.3951932383393455 2306 64-65 0.4364629458584446 2306 66-67 0.42317922873626657 2306 68-69 0.44006869007899496 2306 70-71 0.345134052673167 2306 72-73 0.3499445267274339 2306 74-75 0.36434261308026805 2306 76-77 0.20316018669627312 2306 78-79 0.31974622219964743 2306 80-81 0.4552582393369704 2306 82-83 0.34813364915957123 2306 84-85 0.48129974579254764 2306 86-87 0.49856045983249686 2306 88-89 0.5020747951551456 2306 90-91 0.46480993752778943 2306 92-93 0.5189062737102645 2306 94-95 0.5305382843203859 2306 96-97 0.6508878226630088 2306 98-99 0.485943280399475 2306 100-101 0.3900458727006324 2307 1 0.1400828600987012 2307 2 0.08342151924217234 2307 3 0.22694722029627457 2307 4 0.18135168518724498 2307 5 0.11812715973450594 2307 6 0.1655163039906853 2307 7 0.16688671255695908 2307 8 0.19725167476410377 2307 9 0.19159989436290914 2307 10-11 0.18250506660273658 2307 12-13 0.19280996900466363 2307 14-15 0.4280894082018065 2307 16-17 0.3014465489042166 2307 18-19 0.3209153806110905 2307 20-21 0.3079103671635366 2307 22-23 0.2749443113024199 2307 24-25 0.2297111916400567 2307 26-27 0.2963536690730777 2307 28-29 0.29024936691882885 2307 30-31 0.37648256639955946 2307 32-33 0.4151854021985528 2307 34-35 0.3139001874213534 2307 36-37 0.34807956551188113 2307 38-39 0.3245770600076625 2307 40-41 0.3558616548284448 2307 42-43 0.17303509290515962 2307 44-45 0.1585983872357204 2307 46-47 0.3715436698630441 2307 48-49 0.3236775690460263 2307 50-51 0.1791510786587267 2307 52-53 0.23246662862921852 2307 54-55 0.13889959346056457 2307 56-57 0.21658106645225672 2307 58-59 0.21839972507807914 2307 60-61 0.20865507432662866 2307 62-63 0.20572709378321008 2307 64-65 0.09066109950325796 2307 66-67 0.07981574762912658 2307 68-69 0.22790891068492414 2307 70-71 -0.12489156464852869 2307 72-73 -0.0867897417646688 2307 74-75 -0.2474915582635333 2307 76-77 -0.2474146074178556 2307 78-79 -0.007501709535866041 2307 80-81 -0.03817820377214787 2307 82-83 0.13063944715291598 2307 84-85 0.12193182112481082 2307 86-87 0.07758020593009718 2307 88-89 0.13950898826661984 2307 90-91 0.1367351366952363 2307 92-93 0.24592979354235922 2307 94-95 0.19934000772856564 2307 96-97 0.289235636423129 2307 98-99 -0.02020003954629601 2307 100-101 -0.023156386568430776 2201 1 0.12967657464587745 2201 2 0.09650357235637586 2201 3 0.29721934129187133 2201 4 0.21197383720895857 2201 5 0.14326709066221355 2201 6 0.19856742500508773 2201 7 0.13120651975627595 2201 8 0.1927892414389234 2201 9 0.19629282980978502 2201 10-11 0.19074829883479794 2201 12-13 0.13614131227888748 2201 14-15 0.2577660471016614 2201 16-17 0.3321989949532522 2201 18-19 0.3712288540947313 2201 20-21 0.3698002651847574 2201 22-23 0.33986763918267116 2201 24-25 0.27583629584393066 2201 26-27 0.3273547941616144 2201 28-29 0.3604729900173069 2201 30-31 0.3780935740902791 2201 32-33 0.32573428243773606 2201 34-35 0.43296965458694814 2201 36-37 0.3737854097043538 2201 38-39 0.3433827268184899 2201 40-41 0.26687874739910455 2201 42-43 0.3481031779982118 2201 44-45 0.3490405407617274 2201 46-47 0.3194754970193614 2201 48-49 0.35458303026965154 2201 50-51 0.5050703449380407 2201 52-53 0.3840701931854795 2201 54-55 0.4976083741654591 2201 56-57 0.48586223203144385 2201 58-59 0.5298771951018324 2201 60-61 0.39646079128068124 2201 62-63 0.27118632734406845 2201 64-65 0.4044610625226355 2201 66-67 0.2630290506258106 2201 68-69 0.4382966274759994 2201 70-71 0.33502065337420817 2201 72-73 0.2719479496414081 2201 74-75 0.4315658901986339 2201 76-77 0.18997797639967118 2201 78-79 0.41479220182345244 2201 80-81 0.5055169129048593 2201 82-83 0.5497357879513203 2201 84-85 0.48699186763264635 2201 86-87 0.4795469954476559 2201 88-89 0.5173883844168685 2201 90-91 0.3627620542033725 2201 92-93 0.4590746823811074 2201 94-95 0.47300400426868094 2201 96-97 0.5027992211620358 2201 98-99 0.49854224309851247 2201 100-101 0.4983207387206754 1101 1 0.1574073342602631 1101 2 0.10244799578093478 1101 3 0.32066912478705945 1101 4 0.1981055809964758 1101 5 0.13218540738984075 1101 6 0.14387766744179942 1101 7 0.18174805836758878 1101 8 0.15693861095300576 1101 9 0.15088855104628607 1101 10-11 0.11787368576662516 1101 12-13 0.09583712135638223 1101 14-15 0.13924354586813337 1101 16-17 0.1517047842720487 1101 18-19 0.1171911554844769 1101 20-21 0.1800247796807355 1101 22-23 0.17305493963205265 1101 24-25 0.2525462634733131 1101 26-27 0.15476560900297898 1101 28-29 0.14406885064882857 1101 30-31 0.20229786081921475 1101 32-33 0.13994460961777833 1101 34-35 0.10124113414345004 1101 36-37 0.1560702933095115 1101 38-39 0.1330378458242265 1101 40-41 0.251941574728491 1101 42-43 0.1557298559934921 1101 44-45 0.19388414175563895 1101 46-47 0.2811259341223362 1101 48-49 0.22735962429601386 1101 50-51 0.23160451995181575 1101 52-53 0.16756737098936725 1101 54-55 0.19780374846322957 1101 56-57 0.39195467969747 1101 58-59 0.3340674538234296 1101 60-61 0.28491069646993594 1101 62-63 0.3378204685047663 1101 64-65 0.24729892398167408 1101 66-67 0.11536679370971825 1101 68-69 0.11288513557308733 1101 70-71 0.03656948699185847 1101 72-73 0.18304401494022926 1101 74-75 0.09493840902249673 1101 76-77 -0.13265247411177583 1101 78-79 0.1867605259633507 1101 80-81 0.30157535260987345 1101 82-83 0.28036220264488065 1101 84-85 0.3022992115378784 1101 86-87 0.3249351220891441 1101 88-89 0.45062436249423854 1101 90-91 0.4246441525631539 1101 92-93 0.3584670970790924 1101 94-95 0.2769375991336318 1101 96-97 0.297490925830445 1101 98-99 0.3583026606640516 1101 100-101 0.2390838269179021 2304 1 0.11371360742366221 2304 2 0.05298437945626944 2304 3 0.29229365814331487 2304 4 0.1783429241246779 2304 5 0.07899043235788383 2304 6 0.10880212399663947 2304 7 0.12173919602897598 2304 8 0.11858185277222333 2304 9 0.10124719192288012 2304 10-11 0.11477076497019567 2304 12-13 0.029856927691135127 2304 14-15 0.21633445566049403 2304 16-17 0.1832370456923016 2304 18-19 0.1680670692409123 2304 20-21 0.23337634749622538 2304 22-23 0.3225143810677338 2304 24-25 0.23487435794099554 2304 26-27 0.1977456089423555 2304 28-29 0.3057660904547319 2304 30-31 0.22654128964038023 2304 32-33 0.12206451077624081 2304 34-35 0.22153491129917313 2304 36-37 0.23694202666555242 2304 38-39 0.2573909102616412 2304 40-41 0.32416398525703016 2304 42-43 0.25499045972928513 2304 44-45 0.1853050204443818 2304 46-47 0.32929967509017644 2304 48-49 0.26896425321452 2304 50-51 0.2707392817861134 2304 52-53 0.14805431185391882 2304 54-55 0.18513785173978192 2304 56-57 0.1558821369365475 2304 58-59 0.07864719406541809 2304 60-61 0.1310413046341523 2304 62-63 0.2719199835287114 2304 64-65 0.1677148614206203 2304 66-67 0.12075164269309369 2304 68-69 0.11735509139091249 2304 70-71 0.2562465655285493 2304 72-73 0.07957428260986177 2304 74-75 -0.12012638167227152 2304 76-77 -0.23629671129065954 2304 78-79 0.1535942019682679 2304 80-81 0.2890894032470044 2304 82-83 0.3295752567373924 2304 84-85 0.25899204084223726 2304 86-87 0.3617056980226607 2304 88-89 0.3979616513626567 2304 90-91 0.14338629020001292 2304 92-93 0.271321594068354 2304 94-95 0.07719361765636279 2304 96-97 0.3230249189340242 2304 98-99 0.2720648859928687 2304 100-101 0.21963084209272665 2202 1 0.10808461106635292 2202 2 0.11179170393843663 2202 3 0.30767964184801855 2202 4 0.18211502261834767 2202 5 0.05915870112251298 2202 6 0.034702669043475964 2202 7 0.07870697127555815 2202 8 0.12251166606633745 2202 9 0.08509577433830628 2202 10-11 0.07274170976717187 2202 12-13 0.0869207279817985 2202 14-15 0.05113027058191477 2202 16-17 0.02240569736778042 2202 18-19 0.04922211555864919 2202 20-21 0.034767973771096194 2202 22-23 0.08635980143067457 2202 24-25 0.07603745547001495 2202 26-27 0.08522773352101609 2202 28-29 0.019871753165851658 2202 30-31 0.09558138306960018 2202 32-33 0.0586397623248871 2202 34-35 -0.002541558660936971 2202 36-37 0.04114336796529017 2202 38-39 -0.005557604129577953 2202 40-41 0.16302806638346112 2202 42-43 0.04865496570318584 2202 44-45 0.09987227042311986 2202 46-47 0.03807871432780274 2202 48-49 0.07561573272155186 2202 50-51 0.13228294512861538 2202 52-53 0.07488660233045863 2202 54-55 0.17032041696326417 2202 56-57 0.025394146660055128 2202 58-59 0.012754338261473208 2202 60-61 0.02281643900845154 2202 62-63 0.19783468412526162 2202 64-65 -0.002066349368831766 2202 66-67 -0.14617322378580866 2202 68-69 -0.09695986661687783 2202 70-71 -0.16148053925949313 2202 72-73 -0.20875629588544342 2202 74-75 -0.2442395267872044 2202 76-77 -0.3642099428126251 2202 78-79 0.05023677705279539 2202 80-81 -0.09324436729244212 2202 82-83 -0.11315119521479744 2202 84-85 -0.03301012528005742 2202 86-87 0.031160943345447834 2202 88-89 0.10806840359865788 2202 90-91 -0.1449579913845085 2202 92-93 -0.0803301840626034 2202 94-95 -0.13119979596672948 2202 96-97 -0.07084575389540859 2202 98-99 -0.2560093826823078 2202 100-101 -0.2678339984950995 1102 1 0.1094985018362209 1102 2 0.0837908596436634 1102 3 0.2747310635404361 1102 4 0.13199385722897716 1102 5 0.08539079331223576 1102 6 0.04148139160326281 1102 7 0.026064535666918687 1102 8 1.4396458311694005E-4 1102 9 0.02102809138715145 1102 10-11 0.10101119330821717 1102 12-13 0.09560340595150763 1102 14-15 0.162410164903676 1102 16-17 0.1816313220171537 1102 18-19 0.14698092195206414 1102 20-21 0.05934770736377715 1102 22-23 0.14905840889976218 1102 24-25 0.07428606139896488 1102 26-27 0.09632639734373782 1102 28-29 0.1669768623106478 1102 30-31 0.17497993413453372 1102 32-33 0.12411029002426943 1102 34-35 0.14160460532716002 1102 36-37 0.03179868613868564 1102 38-39 0.039927956258459574 1102 40-41 0.1434434700164502 1102 42-43 0.18559066548569803 1102 44-45 0.16503811728561857 1102 46-47 0.13308384220665204 1102 48-49 0.0830219955507161 1102 50-51 0.13403351863805568 1102 52-53 0.005536396230631624 1102 54-55 0.05940964912463187 1102 56-57 0.16143385628728169 1102 58-59 0.04878557716811116 1102 60-61 0.10285534372786742 1102 62-63 0.08861559872070757 1102 64-65 -0.027480089155368148 1102 66-67 -0.048028059115509336 1102 68-69 -0.13630560633675515 1102 70-71 -0.17842305349055465 1102 72-73 -0.351480742208345 1102 74-75 -0.35247999779462447 1102 76-77 -0.26483077972487123 1102 78-79 -0.020014973773200495 1102 80-81 -0.1259039816739289 1102 82-83 -0.15190796106085003 1102 84-85 -0.07018636322979432 1102 86-87 -0.06310618404762636 1102 88-89 0.0636372648762773 1102 90-91 0.006700729720989784 1102 92-93 0.04141755156607729 1102 94-95 0.023806122176061706 1102 96-97 -0.08719998857401023 1102 98-99 -0.15531687918166526 1102 100-101 -0.26510979707827786 2305 1 0.08582977885649967 2305 2 0.05329091614918724 2305 3 0.23114116919889938 2305 4 0.12387696445632912 2305 5 0.04215800473485842 2305 6 -0.01219576831469027 2305 7 0.015851676839680806 2305 8 0.03486992651532006 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0.14485432900525552 1213 96-97 0.1598403454782087 1213 98-99 0.19448056560728944 1213 100-101 0.268162682289276 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 10.0 10 15.0 11 21.0 12 48.0 13 138.0 14 322.0 15 862.0 16 1612.0 17 2625.0 18 3682.0 19 5111.0 20 6767.0 21 8537.0 22 10882.0 23 13875.0 24 17436.0 25 21943.0 26 27779.0 27 36796.0 28 52977.0 29 74716.0 30 74755.0 31 76571.0 32 99645.0 33 139034.0 34 206919.0 35 336940.0 36 599658.0 37 935420.0 38 356768.0 39 1378.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.3970081944532 25.251161856860264 22.054269751566626 16.29756019711991 2 29.00659248038381 24.99964667080383 31.56382859807969 14.429932250732676 3 36.1038018270355 20.31706517968419 26.886683969112564 16.69244902416775 4 28.662772560397897 20.933364647250965 31.740425186028126 18.663437606323015 5 32.57547432838544 19.081896568338365 31.714668044008114 16.62796105926808 6 27.965524064287926 20.335637039692358 35.09551451797546 16.603324378044253 7 31.991549650461064 22.72186182644213 27.118529739397236 18.168058783699575 8 28.488708241307137 30.11990065667837 24.144525774401234 17.246865327613257 9 31.70862932683722 25.140881986763645 24.55040465829212 18.600084028107016 10-11 26.757432727271556 29.00839848113293 27.688022275175246 16.546146516420265 12-13 25.556855022238867 27.98159347021739 27.202662946644384 19.258888560899358 14-15 24.573065908101004 28.43839095168898 27.85869016370063 19.12985297650939 16-17 24.866891255552087 27.59526718753814 28.11512685802976 19.42271469888001 18-19 22.574427189131338 28.0246264025563 29.740505402082203 19.660441006230155 20-21 23.649591872657588 28.008598105384607 29.7461265483849 18.5956834735729 22-23 22.110345350380502 31.04435437762026 28.09966067548833 18.74563959651091 24-25 21.939028871492244 28.0089996158348 29.773477440252034 20.278494072420923 26-27 21.98240806053107 27.95832899702047 28.23822996205887 21.82103298038959 28-29 22.92186087686084 29.81645821301396 28.081723168324206 19.17995774180099 30-31 21.268313694654193 28.429975292652937 29.642633214743206 20.659077797949664 32-33 21.178359293393 30.064620697895826 28.1658295976801 20.591190411031064 34-35 22.45993246915436 28.573041496265635 28.60061723398487 20.366408800595135 36-37 21.142148182946073 28.54570640455799 29.800118045266743 20.512027367229187 38-39 21.242279916902955 30.032659033109532 28.168596737629997 20.556464312357512 40-41 21.098237395582856 28.32808263047365 30.970431390637515 19.603248583305977 42-43 21.091147195129942 29.777870429020943 29.5164215406003 19.614560835248813 44-45 20.709471016482855 28.314871829463566 30.030322156856133 20.94533499719745 46-47 22.29348098249825 28.149439208671744 28.360929484343085 21.196150324486922 48-49 20.86230871749374 30.021479982692668 29.404592996568407 19.71161830324519 50-51 22.210809904442762 28.29334864065108 29.496053461685236 19.99978799322092 52-53 20.76228386423453 28.459019468741715 28.05469820695871 22.723998460065044 54-55 21.964746302155415 28.423625152522586 29.7397724138939 19.871856131428093 56-57 20.84676846177383 28.242078862430443 29.449405347495667 21.461747328300063 58-59 20.655355455657315 28.591333054691987 29.682210720324292 21.071100769326407 60-61 21.900192894232607 28.43240109151474 29.724840633386922 19.942565380865727 62-63 20.614311996742856 28.657994335333182 29.259329153761094 21.46836451416287 64-65 21.83746945193785 28.55103202858407 29.557979679866747 20.053518839611332 66-67 20.831034818444365 30.863654720600724 27.94095582449831 20.364354636456603 68-69 20.791364098072627 31.169238623546704 27.91689565305257 20.1225016253281 70-71 20.591406624504728 31.114278682604414 28.099863001551196 20.19445169133967 72-73 20.50213748669091 31.26155409065411 27.86988029688993 20.36642812576505 74-75 20.544552984204394 30.851881916009404 28.262178112337764 20.34138698744843 76-77 20.918253831452553 30.259443028734665 28.15764011791828 20.664663021894498 78-79 20.685193023261416 30.338355919040023 28.423728364344004 20.552722693354557 80-81 20.458927895149184 30.129222937432765 28.557919044619478 20.85393012279857 82-83 20.396914953724625 30.216246184405747 28.274826216404865 21.112012645464763 84-85 20.302892857590276 30.01619617350143 28.670086001617044 21.010824967291246 86-87 20.511394639202017 29.712463194328325 28.64164322717012 21.134498939299533 88-89 20.57989763998845 30.152897496385016 28.449767230725094 20.81743763290144 90-91 20.740197650992858 29.60530363551048 28.415089253628867 21.239409459867794 92-93 20.52316795552899 29.94847067445312 28.195699308546075 21.332662061471815 94-95 20.224019303090326 29.98459415477493 28.503946241793056 21.287440300341693 96-97 20.473480975312913 29.972498860612003 28.175631378383965 21.37838878569112 98-99 20.50468016728206 29.934900345524117 28.08053778827615 21.479881698917673 100-101 20.577607875574355 29.63793363522264 28.067352083584 21.717106405619003 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 77.0 1 50.0 2 40.0 3 85.5 4 92.5 5 59.0 6 45.0 7 48.5 8 68.0 9 80.5 10 70.0 11 67.5 12 70.0 13 80.0 14 92.5 15 114.5 16 183.0 17 264.5 18 396.0 19 656.5 20 1044.5 21 1636.5 22 2511.0 23 3713.0 24 5455.5 25 7783.0 26 10788.0 27 14402.5 28 18656.0 29 23901.5 30 30011.5 31 36919.0 32 45510.0 33 56266.0 34 69274.0 35 86527.5 36 107927.5 37 133005.5 38 158125.5 39 174685.5 40 183475.0 41 180712.5 42 171241.5 43 166915.5 44 161342.0 45 156085.5 46 153630.0 47 144057.0 48 126267.5 49 110011.5 50 97828.5 51 86940.0 52 75757.5 53 64231.0 54 53089.5 55 42533.0 56 33887.5 57 26850.0 58 21073.0 59 16573.0 60 12952.0 61 9929.5 62 7305.0 63 5345.0 64 3970.0 65 2885.5 66 2052.5 67 1472.0 68 1079.5 69 796.5 70 577.5 71 419.0 72 297.5 73 221.0 74 167.5 75 120.5 76 91.5 77 72.5 78 59.0 79 46.5 80 32.0 81 25.0 82 23.0 83 15.5 84 13.5 85 12.0 86 7.5 87 3.5 88 1.5 89 2.5 90 1.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16985498084955758 2 0.0 3 1.2848334406169256E-4 4 0.02174580598244147 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 8.030209003855785E-5 12-13 3.212083601542314E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 6.424167203084628E-5 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0010118063344858289 38-39 5.299937942544818E-4 40-41 3.212083601542314E-4 42-43 5.139333762467702E-4 44-45 2.8908752413880825E-4 46-47 0.0031639023475191794 48-49 0.0036296544697428145 50-51 0.004368433698097547 52-53 0.0040311649199356045 54-55 0.005797810900783877 56-57 0.009363223698495847 58-59 0.016734955564035457 60-61 0.0044647962061438165 62-63 0.002778452315334102 64-65 0.01032684877895854 66-67 0.023351847783212625 68-69 0.02555212505026911 70-71 0.02569666881233851 72-73 0.02280579357095043 74-75 0.024556379133790992 76-77 0.024074566593559645 78-79 0.015144974181272012 80-81 0.0031157210934960444 82-83 0.0010599875885089636 84-85 0.005235696270513972 86-87 0.01421346993682474 88-89 0.016221022187788686 90-91 0.023689116561374565 92-93 0.02391396241348253 94-95 0.02534333961616886 96-97 0.026579991802762652 98-99 0.032618708973662196 100-101 0.023592754053328297 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3113244.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.15694317740662 #Duplication Level Percentage of deduplicated Percentage of total 1 94.65953021805545 73.98299524452565 2 3.3253252880523982 5.197945191694092 3 0.748441748162937 1.754877575483086 4 0.32883982237775533 1.0280446124818685 5 0.1684699272294404 0.65835472647866 6 0.15080438155849588 0.7071845688222784 7 0.0987213090669976 0.5401029020203969 8 0.08649717611287479 0.5408283902768071 9 0.04448288529037158 0.3128981704206001 >10 0.320253391693677 4.723102353534352 >50 0.0329169457346323 1.8209323775778465 >100 0.03342351922255361 4.999741573775126 >500 0.0019968069813859498 1.034165796475683 >1k 2.555962475185841E-4 0.2554569460678135 >5k 0.0 0.0 >10k+ 4.0984213454406026E-5 2.4433695703658374 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT 76279 2.4501452504204617 TruSeq Adapter, Index 2 (97% over 37bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0012205917685860794 0.0 0.0 2 0.0012848334406169255 0.0 0.0 3 0.0014454376206940412 0.0 0.0 4 0.0015096792927248876 0.0 0.0 5 0.0018630084888945422 0.0 0.0 6 0.0024090627011567355 0.0 0.0 7 0.002730271061310967 0.0 3.212083601542314E-5 8 0.003212083601542314 0.0 6.424167203084628E-5 9 0.0036938961417736613 0.0 6.424167203084628E-5 10-11 0.005332058778560241 0.0 6.424167203084628E-5 12-13 0.006729315145231148 0.0 6.424167203084628E-5 14-15 0.00838353820002544 0.0 6.424167203084628E-5 16-17 0.009764734148688635 0.0 6.424167203084628E-5 18-19 0.011370775949459792 0.0 1.1242292605398099E-4 20-21 0.012928636496207815 0.0 1.2848334406169256E-4 22-23 0.014775584567094643 0.0 1.2848334406169256E-4 24-25 0.016863438908097148 0.0 1.4454376206940412E-4 26-27 0.019079776593161346 0.0 1.606041800771157E-4 28-29 0.021312174696233255 0.0 1.606041800771157E-4 30-31 0.035878973829227646 0.0 1.9272501609253885E-4 32-33 0.038914392832685135 0.0 1.9272501609253885E-4 34-35 0.04129133469782645 0.0 1.9272501609253885E-4 36-37 0.044117968267183685 0.0 1.9272501609253885E-4 38-39 0.04700884350857176 0.0 1.9272501609253885E-4 40-41 0.05062243756030687 0.0 2.2484585210796198E-4 42-43 0.05495875042238899 0.0 2.8908752413880825E-4 44-45 0.059102338268378574 0.0 3.37268778161943E-4 46-47 0.0637116782365918 0.0 3.5332919616965457E-4 48-49 0.06942918704733711 0.0 4.015104501927893E-4 50-51 0.07546790421823667 0.0 4.4969170421592397E-4 52-53 0.0818920714213213 0.0 4.4969170421592397E-4 54-55 0.08934410537689946 0.0 4.4969170421592397E-4 56-57 0.0989642957635187 0.0 4.4969170421592397E-4 58-59 0.10898599660033072 0.0 4.6575212222363556E-4 60-61 0.1197785975015129 0.0 5.139333762467702E-4 62-63 0.1314545213931192 0.0 5.139333762467702E-4 64-65 0.14568405174795168 0.0 5.299937942544818E-4 66-67 0.16082902592922366 0.0 5.460542122621934E-4 68-69 0.17857578782774497 0.0 5.460542122621934E-4 70-71 0.19900463953355407 0.0 5.460542122621934E-4 72-73 0.22150528516235798 0.0 5.460542122621934E-4 74-75 0.24800497487508205 0.0 5.621146302699049E-4 76-77 0.2788409774498883 0.0 5.781750482776165E-4 78-79 0.3139651116327535 0.0 5.781750482776165E-4 80-81 0.3528634440474309 0.0 5.781750482776165E-4 82-83 0.3955841559479437 0.0 5.781750482776165E-4 84-85 0.4413884681059371 0.0 5.781750482776165E-4 86-87 0.4934724037049457 0.0 5.781750482776165E-4 88-89 0.5518038419089542 0.0 5.781750482776165E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 11295 0.0 72.6771 3 CGGAAGA 11395 0.0 72.53957 4 GATCGGA 11640 0.0 70.809105 1 ATCGGAA 11710 0.0 70.14201 2 GAAGAGC 12455 0.0 66.4644 6 GGAAGAG 15835 0.0 52.457455 5 AGAGCAC 16500 0.0 50.314476 8 TTGTGTT 23000 0.0 50.2567 1 GAGCACA 16735 0.0 49.778214 9 AAGAGCA 17810 0.0 46.4003 7 GCCGTCT 9075 0.0 45.45478 50-51 ATGCCGT 9135 0.0 45.15587 48-49 CGTATGC 9110 0.0 45.018745 46-47 TGCCGTC 9105 0.0 44.992023 50-51 TCTCGTA 9170 0.0 44.983154 42-43 TATGCCG 9125 0.0 44.971127 48-49 TCGTATG 9210 0.0 44.91671 44-45 CGTCTTC 9205 0.0 44.688503 52-53 CTCGTAT 9155 0.0 44.667763 44-45 ATCTCGT 9220 0.0 44.378616 42-43 >>END_MODULE