FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n01_50331580-1.341000000357a8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n01_50331580-1.341000000357a8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3113244
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT762792.4501452504204617TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG112950.072.67713
CGGAAGA113950.072.539574
GATCGGA116400.070.8091051
ATCGGAA117100.070.142012
GAAGAGC124550.066.46446
GGAAGAG158350.052.4574555
AGAGCAC165000.050.3144768
TTGTGTT230000.050.25671
GAGCACA167350.049.7782149
AAGAGCA178100.046.40037
GCCGTCT90750.045.4547850-51
ATGCCGT91350.045.1558748-49
CGTATGC91100.045.01874546-47
TGCCGTC91050.044.99202350-51
TCTCGTA91700.044.98315442-43
TATGCCG91250.044.97112748-49
TCGTATG92100.044.9167144-45
CGTCTTC92050.044.68850352-53
CTCGTAT91550.044.66776344-45
ATCTCGT92200.044.37861642-43
Produced by FastQC (version 0.11.2)