##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n01_50330665-2.34100000035758.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2525090 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.680443865367174 33.0 33.0 33.0 33.0 33.0 2 32.73310852286453 33.0 33.0 33.0 33.0 33.0 3 32.56889497008027 33.0 33.0 33.0 33.0 33.0 4 36.62729486869775 37.0 37.0 37.0 37.0 37.0 5 36.69134605103184 37.0 37.0 37.0 37.0 37.0 6 36.58119789789671 37.0 37.0 37.0 37.0 37.0 7 36.53071058853348 37.0 37.0 37.0 37.0 37.0 8 36.461169700881946 37.0 37.0 37.0 37.0 37.0 9 36.47980428420373 37.0 37.0 37.0 37.0 37.0 10-11 36.434299767533034 37.0 37.0 37.0 37.0 37.0 12-13 36.41579805076255 37.0 37.0 37.0 37.0 37.0 14-15 38.257077569512376 40.0 37.0 40.0 37.0 40.0 16-17 38.265978638385164 40.0 37.0 40.0 37.0 40.0 18-19 38.308407225089006 40.0 37.0 40.0 37.0 40.0 20-21 38.26301636773343 40.0 37.0 40.0 37.0 40.0 22-23 38.16841261103565 40.0 37.0 40.0 37.0 40.0 24-25 38.133644741375555 40.0 37.0 40.0 37.0 40.0 26-27 38.07806791045071 40.0 37.0 40.0 37.0 40.0 28-29 37.959254917646504 40.0 37.0 40.0 33.0 40.0 30-31 37.93235706450067 40.0 37.0 40.0 33.0 40.0 32-33 37.83297664637696 40.0 37.0 40.0 33.0 40.0 34-35 37.75131421058259 40.0 37.0 40.0 33.0 40.0 36-37 37.67869996712989 40.0 37.0 40.0 33.0 40.0 38-39 37.58643117671053 40.0 37.0 40.0 33.0 40.0 40-41 37.521202808612756 40.0 37.0 40.0 33.0 40.0 42-43 37.3976759244225 40.0 37.0 40.0 33.0 40.0 44-45 37.278850456815405 40.0 37.0 40.0 33.0 40.0 46-47 37.23418234597579 40.0 37.0 40.0 33.0 40.0 48-49 37.068091236351975 38.5 37.0 40.0 33.0 40.0 50-51 36.91020498279269 37.0 37.0 40.0 33.0 40.0 52-53 36.79471820806387 37.0 37.0 40.0 33.0 40.0 54-55 36.668473004922596 37.0 37.0 40.0 33.0 40.0 56-57 36.49870420460261 37.0 37.0 40.0 33.0 40.0 58-59 36.292805800981355 37.0 37.0 40.0 33.0 40.0 60-61 36.02881758669988 37.0 37.0 40.0 33.0 40.0 62-63 35.804374101517176 37.0 37.0 40.0 33.0 40.0 64-65 35.54369270006218 37.0 33.0 40.0 27.0 40.0 66-67 35.23303684225117 37.0 33.0 37.0 27.0 40.0 68-69 34.97515217279384 37.0 33.0 37.0 27.0 40.0 70-71 34.69746820905394 37.0 33.0 37.0 27.0 40.0 72-73 34.37736258905623 37.0 33.0 37.0 27.0 40.0 74-75 34.0238781587983 37.0 33.0 37.0 27.0 38.5 76-77 31.426769738900397 33.0 30.0 35.0 24.5 37.0 78-79 33.72558047435933 37.0 33.0 37.0 27.0 37.0 80-81 34.05862127686538 37.0 33.0 37.0 27.0 37.0 82-83 33.98113354375488 37.0 33.0 37.0 27.0 37.0 84-85 33.81441750591068 37.0 33.0 37.0 27.0 37.0 86-87 33.648853308198916 37.0 33.0 37.0 27.0 37.0 88-89 33.53342435319137 37.0 33.0 37.0 27.0 37.0 90-91 33.404002431596496 37.0 33.0 37.0 27.0 37.0 92-93 33.23467282354292 37.0 33.0 37.0 27.0 37.0 94-95 33.117961538004586 37.0 33.0 37.0 27.0 37.0 96-97 32.98682898431343 35.0 33.0 37.0 27.0 37.0 98-99 32.82242949756247 33.0 33.0 37.0 27.0 37.0 100-101 31.246137761426326 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 0.18224562479412043 2306 2 0.14829035108571276 2306 3 0.31381582353044735 2306 4 0.22786406711546903 2306 5 0.17510103187115078 2306 6 0.15295568354787292 2306 7 0.17777812443009822 2306 8 0.18032585683004498 2306 9 0.17744418905588333 2306 10-11 0.21889591854066737 2306 12-13 0.16833552204286661 2306 14-15 0.4173706646690505 2306 16-17 0.4675055640551591 2306 18-19 0.4359584682983453 2306 20-21 0.4434136857517146 2306 22-23 0.48490352092102995 2306 24-25 0.5293020614621966 2306 26-27 0.4107726713025883 2306 28-29 0.5513558222171611 2306 30-31 0.5366655394012838 2306 32-33 0.5787727672265461 2306 34-35 0.45373172008542184 2306 36-37 0.49564183928490024 2306 38-39 0.488585323453826 2306 40-41 0.3866527432697282 2306 42-43 0.5328203893877372 2306 44-45 0.5691155716058987 2306 46-47 0.5807338678880427 2306 48-49 0.4564473687539419 2306 50-51 0.3837959087370848 2306 52-53 0.5142695155972987 2306 54-55 0.4566774468966628 2306 56-57 0.5061919091315801 2306 58-59 0.5250504900206465 2306 60-61 0.6842407544468259 2306 62-63 0.5224656724552901 2306 64-65 0.47947944302578094 2306 66-67 0.4717071272130724 2306 68-69 0.4888963526010244 2306 70-71 0.5873832032017177 2306 72-73 0.41977196562696406 2306 74-75 0.4096129115119993 2306 76-77 0.10712164264249324 2306 78-79 0.3974284602704259 2306 80-81 0.4027619450580886 2306 82-83 0.22622661066140637 2306 84-85 0.27297009388331617 2306 86-87 0.35336682299144684 2306 88-89 0.32948864102490916 2306 90-91 0.41274633667769933 2306 92-93 0.3611480219611991 2306 94-95 0.3571804999949322 2306 96-97 0.45012244511514155 2306 98-99 0.3323447720133714 2306 100-101 0.5467463561914769 2307 1 0.1630946749639648 2307 2 0.14258131965283383 2307 3 0.29831654800253204 2307 4 0.18393868474856134 2307 5 0.1380698000053826 2307 6 0.15964164554031157 2307 7 0.13853877823773786 2307 8 0.18402060461684755 2307 9 0.1712440002504465 2307 10-11 0.17439383007491216 2307 12-13 0.15413204050882712 2307 14-15 0.29319070604352504 2307 16-17 0.26842919117757447 2307 18-19 0.32723889941606643 2307 20-21 0.38606851997264613 2307 22-23 0.41466711927992606 2307 24-25 0.23075166369053335 2307 26-27 0.3220374045281602 2307 28-29 0.43939407625738625 2307 30-31 0.41575108932404703 2307 32-33 0.4683494027327342 2307 34-35 0.35478295240584856 2307 36-37 0.36666128098075035 2307 38-39 0.29053104847030653 2307 40-41 0.39092329979111895 2307 42-43 0.3789589113211136 2307 44-45 0.2648914387289878 2307 46-47 0.41488479977090975 2307 48-49 0.24415341854449224 2307 50-51 0.29004347837553723 2307 52-53 0.13670469897667203 2307 54-55 0.22686933927038666 2307 56-57 0.27001012227592724 2307 58-59 0.23776380846172174 2307 60-61 0.22734684324280607 2307 62-63 0.23662883242183597 2307 64-65 0.14027904244237277 2307 66-67 0.09997398180775718 2307 68-69 0.31371110670527 2307 70-71 0.30430934773000473 2307 72-73 0.2104303438384818 2307 74-75 -0.0072427001853228035 2307 76-77 -0.25323112919606316 2307 78-79 0.04312392355566175 2307 80-81 0.051163559835167405 2307 82-83 0.10140503658877975 2307 84-85 0.1749979576418852 2307 86-87 0.18528391610555417 2307 88-89 0.14592560238200747 2307 90-91 0.10890836955472594 2307 92-93 0.09020294185049238 2307 94-95 0.15830872616901814 2307 96-97 0.148403155014023 2307 98-99 0.07456372650025145 2307 100-101 -0.09796439937726831 2201 1 0.12704690733071544 2201 2 0.09214412157107432 2201 3 0.2685013992126528 2201 4 0.19350859203310478 2201 5 0.1457530091632222 2201 6 0.16762348218176726 2201 7 0.1270603674358739 2201 8 0.15981954615127592 2201 9 0.015974727609133765 2201 10-11 0.09515370539062928 2201 12-13 0.15434823297916012 2201 14-15 0.22489409529261195 2201 16-17 0.2524184501612723 2201 18-19 0.25730130006193264 2201 20-21 0.30067528634490515 2201 22-23 0.3388323584026338 2201 24-25 0.21692505164725873 2201 26-27 0.22777642378551377 2201 28-29 0.2959159356296439 2201 30-31 0.4844961731391777 2201 32-33 0.3741959863864608 2201 34-35 0.4289311195032255 2201 36-37 0.34551882742756135 2201 38-39 0.32996157676173965 2201 40-41 0.4780080467076502 2201 42-43 0.44063864969897537 2201 44-45 0.41587940906727283 2201 46-47 0.4708600352292436 2201 48-49 0.4952309239439927 2201 50-51 0.4175123405436665 2201 52-53 0.538991951492136 2201 54-55 0.5924391061494845 2201 56-57 0.5929016872594488 2201 58-59 0.5629203419151736 2201 60-61 0.443682644130881 2201 62-63 0.5580236700753858 2201 64-65 0.4226881019971529 2201 66-67 0.46609978614745273 2201 68-69 0.576487470087315 2201 70-71 0.4439585149502321 2201 72-73 0.5046826556087609 2201 74-75 0.5330911563001095 2201 76-77 0.16788840210504574 2201 78-79 0.4534556367307445 2201 80-81 0.5890912446504757 2201 82-83 0.5794648248424821 2201 84-85 0.5807986701995063 2201 86-87 0.740030338027104 2201 88-89 0.6581332971320748 2201 90-91 0.6836995297557422 2201 92-93 0.5291536983645884 2201 94-95 0.5839645764755446 2201 96-97 0.6330580102501671 2201 98-99 0.5990958546159675 2201 100-101 0.44431319494567134 1101 1 0.15995858392673767 1101 2 0.12021240620916274 1101 3 0.3216862497827009 1101 4 0.14544607904299056 1101 5 0.09904876080316427 1101 6 0.16601466135340814 1101 7 0.12217243794166421 1101 8 0.1245572773622925 1101 9 0.15990155269746964 1101 10-11 0.1390505262439845 1101 12-13 0.12333838731865399 1101 14-15 0.2638847549117571 1101 16-17 0.20453565003687402 1101 18-19 0.13728188586336643 1101 20-21 0.21852059159371606 1101 22-23 0.18756230934883433 1101 24-25 0.19252785808504314 1101 26-27 0.3193677369343959 1101 28-29 0.30257078118336267 1101 30-31 0.2768313152347588 1101 32-33 0.2572574757945958 1101 34-35 0.3637816895576407 1101 36-37 0.2607987966315477 1101 38-39 0.31841723899322716 1101 40-41 0.309364712153652 1101 42-43 0.2698047715838854 1101 44-45 0.2511508197715955 1101 46-47 0.3106224444026182 1101 48-49 0.2489319671641539 1101 50-51 0.18785792057084905 1101 52-53 0.2359976226050975 1101 54-55 0.39565234614325817 1101 56-57 0.2743289876752826 1101 58-59 0.2638360701247322 1101 60-61 0.19942290747012947 1101 62-63 0.2736199860137276 1101 64-65 0.11430707530016093 1101 66-67 0.0401048931182828 1101 68-69 0.1139389801702464 1101 70-71 0.1977464193888281 1101 72-73 0.15661074024987442 1101 74-75 0.07937698622714606 1101 76-77 -0.003455443823209947 1101 78-79 0.20011935853731444 1101 80-81 0.26808577981663717 1101 82-83 0.24927420939624056 1101 84-85 0.17864333994194936 1101 86-87 0.22808190676413886 1101 88-89 0.338267576501714 1101 90-91 0.27342508476897365 1101 92-93 0.31826351542731146 1101 94-95 0.24194572902363376 1101 96-97 0.3061470243766422 1101 98-99 0.08501053319820073 1101 100-101 0.1833247950204715 2304 1 0.11250404402259306 2304 2 0.09638931475305412 2304 3 0.30842557119685665 2304 4 0.2137015171253367 2304 5 0.12515121688639397 2304 6 0.06465586154742908 2304 7 0.1268990668224319 2304 8 0.13706325560657007 2304 9 0.037102479367732144 2304 10-11 0.1202355923353835 2304 12-13 0.15715235453244247 2304 14-15 0.36454482091835416 2304 16-17 0.36581935291898304 2304 18-19 0.24286107173673344 2304 20-21 0.189576561527808 2304 22-23 0.27616073753697634 2304 24-25 0.31785729750380654 2304 26-27 0.2688553886800378 2304 28-29 0.2351802805711216 2304 30-31 0.1475681062828258 2304 32-33 0.28873187194952976 2304 34-35 0.2670587849958608 2304 36-37 0.2876678247471105 2304 38-39 0.1949075601733412 2304 40-41 0.35402092494862103 2304 42-43 0.2802849305967001 2304 44-45 0.3390140511101478 2304 46-47 0.31935407296116836 2304 48-49 0.2971861502391846 2304 50-51 0.3202366003709898 2304 52-53 0.2552297616463335 2304 54-55 0.3183311762050849 2304 56-57 0.34361302296473895 2304 58-59 0.2174711818373396 2304 60-61 0.2894272830361544 2304 62-63 0.22795637545945624 2304 64-65 0.2584260092901616 2304 66-67 0.10508798443871825 2304 68-69 0.29841572691994855 2304 70-71 0.22828549590695957 2304 72-73 0.21750149016762066 2304 74-75 0.3412758122865185 2304 76-77 0.06461598305226701 2304 78-79 0.18592590424841404 2304 80-81 0.2559088170021582 2304 82-83 0.26491261804697075 2304 84-85 0.27162327000073816 2304 86-87 0.3904790046445399 2304 88-89 0.38993147876038137 2304 90-91 0.41365434407723 2304 92-93 0.40153325041725196 2304 94-95 0.3710961569132891 2304 96-97 0.2590638987978906 2304 98-99 0.3037108204931158 2304 100-101 0.0974161527687869 2202 1 0.11860007104815651 2202 2 0.10959683851294955 2202 3 0.3054884742722308 2202 4 0.16345127561168482 2202 5 0.11546756768144206 2202 6 0.1265400445210787 2202 7 0.18505679470307257 2202 8 0.1599591347718814 2202 9 0.1024194077419267 2202 10-11 0.14747839634095783 2202 12-13 0.09685565854186251 2202 14-15 0.057701192557601644 2202 16-17 0.07112456713239368 2202 18-19 0.15627029897006395 2202 20-21 0.14901788419260953 2202 22-23 0.1383184791083849 2202 24-25 0.18547840301596352 2202 26-27 0.18045208698093518 2202 28-29 0.1496830576572279 2202 30-31 0.1885654228928928 2202 32-33 0.12617724793643248 2202 34-35 0.11019855658255295 2202 36-37 0.1888652104955284 2202 38-39 0.20676772013282374 2202 40-41 0.23659619634664608 2202 42-43 0.26749839785994567 2202 44-45 0.21471794895650476 2202 46-47 0.2676346412305932 2202 48-49 0.31878044409948103 2202 50-51 0.2798776156781244 2202 52-53 0.318144816439343 2202 54-55 0.2969874875193952 2202 56-57 0.17765014718532512 2202 58-59 0.2630305180406083 2202 60-61 0.27048076173118574 2202 62-63 0.21816769241691958 2202 64-65 0.14157950159039245 2202 66-67 0.18650343412473092 2202 68-69 0.24565921974213722 2202 70-71 0.2799593630603354 2202 72-73 0.27080445139451825 2202 74-75 0.0955754612039712 2202 76-77 -0.13315492509896032 2202 78-79 0.10368323287212178 2202 80-81 0.14215642218788815 2202 82-83 0.24973212670252565 2202 84-85 0.3319964417488137 2202 86-87 0.4834451816096532 2202 88-89 0.41245973949416026 2202 90-91 0.2917527353267815 2202 92-93 0.4217836031683717 2202 94-95 0.3894086246651227 2202 96-97 0.40821408734539943 2202 98-99 0.2686901645514581 2202 100-101 0.26572496251935007 1102 1 0.12072605188514274 1102 2 0.09394765120644166 1102 3 0.2904915101962189 1102 4 0.2091594465842519 1102 5 0.030964625170497584 1102 6 -0.03453907632871278 1102 7 0.019981764010218228 1102 8 0.08874002780059698 1102 9 0.05279789738641938 1102 10-11 0.0070560692885379694 1102 12-13 0.0753222833950673 1102 14-15 0.07503890407141967 1102 16-17 -0.030294894141228212 1102 18-19 0.0690562930183134 1102 20-21 0.05733423080839373 1102 22-23 0.07769091024962194 1102 24-25 0.029251756277410834 1102 26-27 0.0946396937386993 1102 28-29 0.080826800317638 1102 30-31 0.11966621223312757 1102 32-33 0.10585031895704589 1102 34-35 0.04886747072262665 1102 36-37 0.036901785039141544 1102 38-39 0.17164274682226477 1102 40-41 0.03697668769926565 1102 42-43 0.0060640572226020595 1102 44-45 0.025868047040198405 1102 46-47 0.08806349051531726 1102 48-49 0.04643679418488489 1102 50-51 0.11540726764780374 1102 52-53 -0.09128831650076563 1102 54-55 0.01670122657672124 1102 56-57 -0.0075149436922217205 1102 58-59 -0.030401543985661306 1102 60-61 -0.019571749745274758 1102 62-63 0.018693307608550924 1102 64-65 -0.13989318843843535 1102 66-67 -0.2202399035233924 1102 68-69 -0.24619549628220483 1102 70-71 -0.24578593959793693 1102 72-73 -0.22135844427644713 1102 74-75 -0.249123265042428 1102 76-77 -0.2982433005126488 1102 78-79 -0.0824672822340986 1102 80-81 0.03600125311026403 1102 82-83 -0.06698397103421172 1102 84-85 0.011858674187159579 1102 86-87 -0.04252030894227943 1102 88-89 -0.09240941551811943 1102 90-91 -0.02935711174272626 1102 92-93 -0.013455300696264771 1102 94-95 -0.011675717306694366 1102 96-97 -0.070326350494895 1102 98-99 -0.14477265705632192 1102 100-101 -0.3172892095741702 2305 1 0.09993419497992306 2305 2 0.06380018683562128 2305 3 0.29004596118841164 2305 4 0.1350399635022086 2305 5 0.09314590186288285 2305 6 -0.04734884072785661 2305 7 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88-89 -0.2744246343963681 1213 90-91 -0.15792457070304522 1213 92-93 -0.15421923708477436 1213 94-95 -0.08437899193049958 1213 96-97 -0.10027141718435928 1213 98-99 -0.08361499631760694 1213 100-101 0.07887187113718142 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 5.0 9 17.0 10 11.0 11 19.0 12 26.0 13 49.0 14 162.0 15 370.0 16 685.0 17 1258.0 18 1971.0 19 2660.0 20 3730.0 21 4820.0 22 6184.0 23 8071.0 24 10293.0 25 12853.0 26 16769.0 27 21981.0 28 29764.0 29 37076.0 30 41379.0 31 50768.0 32 69166.0 33 98930.0 34 152564.0 35 266733.0 36 532567.0 37 870086.0 38 283724.0 39 399.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.08156406496944 25.927636130522814 23.58660846329125 13.404191341216496 2 31.45773021951693 22.78607891203878 33.6913139729673 12.064876895476994 3 38.54353706204531 19.090131440859533 26.877893461223163 15.488438035871987 4 30.64735143802619 18.94753896015927 34.62061032235268 15.78449927946185 5 30.68041139127714 17.432764772740775 36.51046101327081 15.37636282271127 6 25.537822414250584 18.29566470898067 40.741201303715904 15.425311573052841 7 34.092091767026126 19.105220011959968 29.714901251044516 17.087786969969386 8 30.20977470109976 28.72594640191043 24.80054176286786 16.263737134121953 9 29.805828703135333 26.636476323616186 25.784625498497082 17.773069474751395 10-11 24.197117090572682 28.99637557186126 30.883834543588186 15.922672793977874 12-13 24.19883021944969 27.76250658887667 30.004704784663033 18.033958407010605 14-15 22.874293589535423 28.448827566542185 30.993528943522808 17.683349900399588 16-17 21.811816608516924 29.106903120284823 31.985453983818395 17.095826287379857 18-19 20.435826049764565 29.35123104523007 32.4132605174469 17.799682387558462 20-21 21.008775924818522 29.367329481325417 31.926584794997403 17.697309798858655 22-23 20.298702224475168 30.18688442788178 31.65778249488137 17.856630852761686 24-25 20.335512793603396 29.211434047895324 31.866190908046843 18.586862250454438 26-27 20.248783211687503 29.332023809052348 31.049843767944907 19.36934921131524 28-29 20.392655622299106 29.95927270653538 30.75168073350315 18.89639093766237 30-31 19.398476885972382 30.16502382093312 30.969252581096118 19.467246711998385 32-33 19.259313529418755 30.85547445833614 31.09241650792645 18.792795504318658 34-35 19.801353615118668 30.007821503391956 31.7884511047131 18.402373776776273 36-37 20.14296689979644 30.299181009560165 31.412540692102365 18.145311398541025 38-39 20.653050555974136 30.53646974492812 30.666624423655165 18.143855275442586 40-41 20.37125116283306 30.22744202863827 30.63887404889265 18.76243275963602 42-43 19.791883656663277 30.67877507150274 31.304894343857892 18.224446927976086 44-45 19.517948137109265 30.211981318619085 31.55160171099462 18.718468833277033 46-47 20.341722433938184 29.82933680263683 30.86872191215652 18.960218851268465 48-49 19.962451381053334 30.781511077456454 30.623886878102237 18.63215066338798 50-51 20.493795183633573 30.11326057218807 30.193201989406624 19.19974225477173 52-53 19.908997353049916 29.887070459724157 30.01204167238453 20.191890514841397 54-55 20.662796210126263 29.987872974357003 30.26387964224086 19.08545117327587 56-57 20.131734323700634 30.265466782343296 30.169797903205907 19.43300099075016 58-59 20.127433668979034 30.29119264732327 30.628660356178944 18.95271332751875 60-61 20.37017418202607 30.699490509504358 30.325229155870346 18.605106152599223 62-63 19.873145333167262 30.605000246533145 30.20129975439755 19.32055466590205 64-65 20.355046248731597 30.246638778276207 30.34761603373856 19.050698939253635 66-67 20.382201810731928 30.504764500274113 29.874838780211878 19.23819490878208 68-69 20.36092428369915 30.754897180113154 29.972968508550135 18.911210027637562 70-71 19.659458023768774 31.431048350598832 30.05583600207016 18.853657623562235 72-73 19.5514731251072 31.44275114862919 29.937184372067527 19.068591354196084 74-75 20.064172012683876 30.717458946747133 29.771377301725316 19.446991738843675 76-77 20.094103032442096 30.31144792148204 30.052799264161507 19.541649781914362 78-79 19.895539919352682 30.42579573425954 30.348618140833956 19.330046205553817 80-81 19.268825499820593 30.73529330227334 30.215136122448815 19.780745075457247 82-83 18.77483355847641 30.633358956476698 30.15580613696065 20.436001348086243 84-85 18.805143298565064 30.46736863283333 30.60275715750238 20.124730911099224 86-87 18.9766508584351 30.403433700783783 31.168938419191367 19.45097702158975 88-89 19.120368426597885 30.782569844439816 30.750882685423353 19.34617904353895 90-91 19.423191720541745 30.540761723152148 30.520619224381097 19.515427331925007 92-93 18.822385563568577 31.513918388656453 30.38375489502484 19.279941152750126 94-95 18.628960332649818 31.698902318182153 30.28828185393569 19.383855495232343 96-97 19.151954353006793 31.708799183652776 30.042096865175942 19.097149598164485 98-99 19.37742671186559 30.985532726763488 29.813184040084796 19.82385652128613 100-101 19.265952927265722 30.92078720616677 29.507337598868688 20.30592226769882 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 114.0 1 87.0 2 85.0 3 193.5 4 240.0 5 167.0 6 132.5 7 132.0 8 138.5 9 140.0 10 136.0 11 145.5 12 160.0 13 170.0 14 186.5 15 198.0 16 228.0 17 306.0 18 426.0 19 621.5 20 1012.5 21 1654.0 22 2551.5 23 3829.5 24 5689.0 25 8344.0 26 12286.0 27 17700.5 28 23334.5 29 29084.5 30 35882.0 31 43052.5 32 51756.0 33 64363.0 34 80376.0 35 99937.5 36 128286.5 37 159762.5 38 184868.0 39 193967.5 40 186521.5 41 169934.5 42 151132.0 43 140053.0 44 126305.5 45 110309.5 46 103372.5 47 92299.5 48 71844.0 49 53660.5 50 40561.0 51 32118.5 52 26574.0 53 20578.0 54 14857.0 55 10387.5 56 7071.5 57 4831.5 58 3359.5 59 2425.5 60 1665.5 61 1103.0 62 773.0 63 525.5 64 351.5 65 218.0 66 148.5 67 109.0 68 80.5 69 59.5 70 38.5 71 32.5 72 22.0 73 16.5 74 14.0 75 11.0 76 10.5 77 7.5 78 5.5 79 6.0 80 4.0 81 1.0 82 1.0 83 1.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16926129365685977 2 0.0 3 0.0 4 0.02328629870618473 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 7.920509764008411E-5 12-13 3.9602548820042056E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 7.920509764008411E-5 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0011880764646012617 38-39 6.93044604350736E-4 40-41 2.772178417402944E-4 42-43 4.752305858405047E-4 44-45 3.564229393803785E-4 46-47 0.00346522302175368 48-49 0.0038414472355440795 50-51 0.0045938956631248785 52-53 0.0042968765469745636 54-55 0.005979984871826351 56-57 0.009326400247119905 58-59 0.015999429723296994 60-61 0.0046136969375349 62-63 0.002950389887093133 64-65 0.010455072888491105 66-67 0.023207093608544647 68-69 0.02483079811016637 70-71 0.025484240165697062 72-73 0.02213782479040351 74-75 0.024751593012526284 76-77 0.024672387914886202 78-79 0.015583602960686551 80-81 0.0033266141008835327 82-83 0.0011484739157812196 84-85 0.005326542816295656 86-87 0.01388069336142474 88-89 0.01615783991857716 90-91 0.022850670669164266 92-93 0.02328629870618473 94-95 0.025088214677496643 96-97 0.026692117904708348 98-99 0.03330574355765537 100-101 0.02346451017587492 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2525090.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.19558654557985 #Duplication Level Percentage of deduplicated Percentage of total 1 91.97237475135752 55.363310441478454 2 4.009482650727171 4.827063198096967 3 1.1384723890272448 2.0559303967032774 4 0.6020815644249145 1.4497061167535221 5 0.4059142035496316 1.2217121784925984 6 0.2781118942825996 1.0044665160986026 7 0.24977424155399275 1.0524714882023956 8 0.16600290878558055 0.799411397009633 9 0.13067598415795675 0.7079505762428197 >10 0.8333768481779641 9.841303916116523 >50 0.10400822663024774 4.367620359691772 >100 0.10342052772924881 12.955321988108675 >500 0.005170266384617109 2.0927718011999943 >1k 9.353794081101918E-4 0.8580385837710912 >5k 1.3210920209504E-4 0.5592121286624198 >10k+ 6.605460104501175E-5 0.8437089133712642 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT 21219 0.8403264834124725 TruSeq Adapter, Index 6 (97% over 36bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 7347 0.290959926180849 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 6717 0.2660103204242225 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 3643 0.14427208535141323 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 3.564229393803785E-4 0.0 0.0 2 3.9602548820042056E-4 0.0 0.0 3 5.544356834805888E-4 0.0 3.9602548820042056E-5 4 6.73243329940715E-4 0.0 3.9602548820042056E-5 5 8.712560740409252E-4 0.0 3.9602548820042056E-5 6 9.108586228609673E-4 0.0 3.9602548820042056E-5 7 9.504611716810094E-4 0.0 3.9602548820042056E-5 8 0.0011484739157812196 0.0 3.9602548820042056E-5 9 0.0012276790134213037 0.0 3.9602548820042056E-5 10-11 0.0019009223433620187 0.0 3.9602548820042056E-5 12-13 0.002772178417402944 0.0 3.9602548820042056E-5 14-15 0.003940453607594185 0.0 7.920509764008411E-5 16-17 0.004910716053685215 0.0 9.900637205010514E-5 18-19 0.006217600164746603 0.0 1.1880764646012617E-4 20-21 0.0077422982943182225 0.0 1.386089208701472E-4 22-23 0.009900637205010514 0.0 1.7821146969018925E-4 24-25 0.012395597780673165 0.0 1.9801274410021028E-4 26-27 0.015048968551615981 0.0 1.9801274410021028E-4 28-29 0.017464724029638546 0.0 2.3761529292025234E-4 30-31 0.025147618500726707 0.0 2.3761529292025234E-4 32-33 0.02829602113192005 0.0 2.3761529292025234E-4 34-35 0.031068199549322995 0.0 2.3761529292025234E-4 36-37 0.034295807278156426 0.0 2.3761529292025234E-4 38-39 0.03817685706252054 0.0 2.3761529292025234E-4 40-41 0.04223611831657485 0.0 2.5741656733027337E-4 42-43 0.04671120633323961 0.0 2.772178417402944E-4 44-45 0.05185953767984507 0.0 2.772178417402944E-4 46-47 0.056651446087070165 0.0 2.772178417402944E-4 48-49 0.06249282203802636 0.0 2.772178417402944E-4 50-51 0.06785896740314207 0.0 2.970191161503154E-4 52-53 0.07445279178167907 0.0 3.1682039056033645E-4 54-55 0.08126443017872631 0.0 3.1682039056033645E-4 56-57 0.08787805583167332 0.0 3.366216649703575E-4 58-59 0.09508571971692098 0.0 3.564229393803785E-4 60-61 0.10280821673682919 0.0 3.564229393803785E-4 62-63 0.11156038002605848 0.0 3.564229393803785E-4 64-65 0.12227286948187986 0.0 3.7622421379039954E-4 66-67 0.13441105069522274 0.0 3.9602548820042056E-4 68-69 0.14639082171328546 0.0 4.356280370204626E-4 70-71 0.16082595075819078 0.0 4.752305858405047E-4 72-73 0.17642935499328738 0.0 4.752305858405047E-4 74-75 0.19460692490168668 0.0 4.752305858405047E-4 76-77 0.21371515470735697 0.0 4.752305858405047E-4 78-79 0.23678363939503144 0.0 4.752305858405047E-4 80-81 0.26531727581987175 0.0 4.752305858405047E-4 82-83 0.29668249448534506 0.0 5.148331346605467E-4 84-85 0.3317505514654923 0.0 5.346344090705678E-4 86-87 0.3719867410666551 0.0 5.544356834805888E-4 88-89 0.4169554352518128 0.0 5.544356834805888E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGACGCC 30 1.2919582E-4 63.427853 1 TCGGAAG 4180 0.0 58.4059 3 CGGAAGA 4240 0.0 57.915474 4 ATCGGAA 4300 0.0 56.886433 2 GATCGGA 4325 0.0 56.535107 1 CGGACCC 35 2.7681218E-4 54.366726 1 GAAGAGC 4740 0.0 51.701942 6 GCCGTCT 2725 0.0 43.75077 50-51 TATGCCG 2700 0.0 43.627247 48-49 ATGCCGT 2720 0.0 43.131832 48-49 TGCCGTC 2765 0.0 43.117844 50-51 AATCTCG 2750 0.0 42.487328 40-41 CGTATGC 2810 0.0 42.341995 46-47 CGGCTCG 45 9.5568696E-4 42.285233 1 CCGGTGC 45 9.5568696E-4 42.285233 1 CTCGTAT 2760 0.0 42.16255 44-45 TCTCGTA 2785 0.0 41.6988 42-43 CACTCCG 2925 0.0 41.406357 30-31 TCGTATG 2905 0.0 41.36607 44-45 GGAAGAG 6055 0.0 41.336334 5 >>END_MODULE