FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n01_50330665-2.34100000035758.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n01_50330665-2.34100000035758.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2525090
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT212190.8403264834124725TruSeq Adapter, Index 6 (97% over 36bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC73470.290959926180849No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG67170.2660103204242225No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA36430.14427208535141323No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCC301.2919582E-463.4278531
TCGGAAG41800.058.40593
CGGAAGA42400.057.9154744
ATCGGAA43000.056.8864332
GATCGGA43250.056.5351071
CGGACCC352.7681218E-454.3667261
GAAGAGC47400.051.7019426
GCCGTCT27250.043.7507750-51
TATGCCG27000.043.62724748-49
ATGCCGT27200.043.13183248-49
TGCCGTC27650.043.11784450-51
AATCTCG27500.042.48732840-41
CGTATGC28100.042.34199546-47
CGGCTCG459.5568696E-442.2852331
CCGGTGC459.5568696E-442.2852331
CTCGTAT27600.042.1625544-45
TCTCGTA27850.041.698842-43
CACTCCG29250.041.40635730-31
TCGTATG29050.041.3660744-45
GGAAGAG60550.041.3363345
Produced by FastQC (version 0.11.2)