##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n01_50330665-1.341000000356c2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2869102 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.658259274156165 33.0 33.0 33.0 33.0 33.0 2 32.685084740800434 33.0 33.0 33.0 33.0 33.0 3 32.53628208408066 33.0 33.0 33.0 33.0 33.0 4 36.562028467443824 37.0 37.0 37.0 37.0 37.0 5 36.647350285908274 37.0 37.0 37.0 37.0 37.0 6 36.542721381115065 37.0 37.0 37.0 37.0 37.0 7 36.346203446235094 37.0 37.0 37.0 37.0 37.0 8 36.30727488949504 37.0 37.0 37.0 37.0 37.0 9 36.40531183624702 37.0 37.0 37.0 37.0 37.0 10-11 36.29856902961275 37.0 37.0 37.0 35.0 37.0 12-13 36.293875226464586 37.0 37.0 37.0 35.0 37.0 14-15 38.07917390179924 40.0 37.0 40.0 35.0 40.0 16-17 38.090090557951584 40.0 37.0 40.0 37.0 40.0 18-19 38.05762133935984 40.0 37.0 40.0 37.0 40.0 20-21 38.088861776263094 40.0 37.0 40.0 37.0 40.0 22-23 37.760776542625536 40.0 37.0 40.0 33.0 40.0 24-25 37.93208902994735 40.0 37.0 40.0 33.0 40.0 26-27 37.87329972932297 40.0 37.0 40.0 33.0 40.0 28-29 37.590004991108714 40.0 37.0 40.0 33.0 40.0 30-31 37.51549456938094 40.0 37.0 40.0 33.0 40.0 32-33 37.48106620120163 40.0 37.0 40.0 33.0 40.0 34-35 37.30272607944925 40.0 37.0 40.0 33.0 40.0 36-37 37.2720860046105 40.0 37.0 40.0 33.0 40.0 38-39 37.24673660957331 40.0 37.0 40.0 33.0 40.0 40-41 37.19666135954735 40.0 37.0 40.0 33.0 40.0 42-43 36.48162787520276 37.0 37.0 40.0 33.0 40.0 44-45 36.27671463057082 37.0 37.0 40.0 33.0 40.0 46-47 36.71641771536878 37.0 37.0 40.0 33.0 40.0 48-49 36.40075082726233 37.0 37.0 40.0 33.0 40.0 50-51 36.376111759010314 37.0 37.0 40.0 33.0 40.0 52-53 36.36858971901313 37.0 37.0 40.0 33.0 40.0 54-55 36.011290640764955 37.0 37.0 40.0 33.0 40.0 56-57 35.900321424613 37.0 37.0 40.0 33.0 40.0 58-59 35.77565837673251 37.0 37.0 40.0 30.0 40.0 60-61 35.465340200522675 37.0 33.0 40.0 27.0 40.0 62-63 35.04647290336837 37.0 33.0 37.0 27.0 40.0 64-65 34.732363296947966 37.0 33.0 37.0 27.0 40.0 66-67 33.86369149650309 37.0 33.0 37.0 27.0 40.0 68-69 33.64190433104156 37.0 33.0 37.0 27.0 37.0 70-71 33.13712774937942 37.0 33.0 37.0 27.0 37.0 72-73 31.669821079905837 33.0 30.0 37.0 18.5 37.0 74-75 30.530329524708428 33.0 27.0 37.0 15.0 37.0 76-77 27.951842945981006 30.0 24.5 33.0 15.0 35.0 78-79 29.78438671751649 33.0 27.0 37.0 15.0 37.0 80-81 30.043660873681034 33.0 27.0 37.0 10.5 37.0 82-83 29.84055760304095 33.0 27.0 37.0 6.0 37.0 84-85 29.65843929564024 33.0 27.0 37.0 6.0 37.0 86-87 29.478516274430117 33.0 27.0 37.0 6.0 37.0 88-89 29.384678899530236 33.0 27.0 37.0 6.0 37.0 90-91 29.247814821501642 33.0 27.0 37.0 6.0 37.0 92-93 29.03229477376545 33.0 27.0 37.0 6.0 37.0 94-95 28.859277397596877 33.0 27.0 37.0 6.0 37.0 96-97 28.707153144084806 33.0 27.0 37.0 6.0 37.0 98-99 28.527030757358922 33.0 27.0 37.0 6.0 37.0 100-101 27.005321002878254 33.0 18.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 0.16476381061588086 2306 2 0.14902216132095703 2306 3 0.3555847299067878 2306 4 0.2280751799278775 2306 5 0.1396304058411033 2306 6 0.2305360209648626 2306 7 0.2543713731040924 2306 8 0.16521585595727828 2306 9 0.1582384878541987 2306 10-11 0.21815470421530847 2306 12-13 0.20511862068953945 2306 14-15 0.30326545596256693 2306 16-17 0.34047728507623987 2306 18-19 0.38573247461382465 2306 20-21 0.4165149612244008 2306 22-23 0.49573144368317656 2306 24-25 0.4147483287538236 2306 26-27 0.3979626219442167 2306 28-29 0.4692223702661238 2306 30-31 0.47403999012848175 2306 32-33 0.5553395572136495 2306 34-35 0.5301726269939238 2306 36-37 0.40725547842743737 2306 38-39 0.46360901990393444 2306 40-41 0.47819138849160225 2306 42-43 0.6233386878886336 2306 44-45 0.6917380433809868 2306 46-47 0.5761542112757354 2306 48-49 0.6752519766571154 2306 50-51 0.6012105395823966 2306 52-53 0.5656894024671502 2306 54-55 0.6311179250921981 2306 56-57 0.6613252930972209 2306 58-59 0.6048318637445149 2306 60-61 0.5878824489285392 2306 62-63 0.6509400831657572 2306 64-65 0.5537227506036899 2306 66-67 0.3927156577420021 2306 68-69 0.35658007729562513 2306 70-71 0.3781251894641855 2306 72-73 0.053090995459751156 2306 74-75 0.08332478061428006 2306 76-77 0.2300140130929158 2306 78-79 0.4514948928262221 2306 80-81 0.5824878823687314 2306 82-83 0.5755556703007443 2306 84-85 0.7225169619563196 2306 86-87 0.7413083718774978 2306 88-89 0.8313268167524797 2306 90-91 0.7877916333244954 2306 92-93 0.7199654345861326 2306 94-95 0.5906918425115926 2306 96-97 0.6670360634864423 2306 98-99 0.5625417068558143 2306 100-101 0.48301866883777933 2307 1 0.13030129953834546 2307 2 0.15262231215731958 2307 3 0.30660500980784633 2307 4 0.2578621986725409 2307 5 0.1702810971348896 2307 6 0.1913519681942759 2307 7 0.20604484242731047 2307 8 0.23860806132240953 2307 9 0.20811824566223436 2307 10-11 0.21152606680994523 2307 12-13 0.24473866010620782 2307 14-15 0.3262568342574639 2307 16-17 0.3586643118844819 2307 18-19 0.45183791223294634 2307 20-21 0.4877312849122646 2307 22-23 0.4823996598218727 2307 24-25 0.4817500489839972 2307 26-27 0.5534126131296233 2307 28-29 0.460856604743924 2307 30-31 0.4256510062421981 2307 32-33 0.4497591455442276 2307 34-35 0.4285941616511053 2307 36-37 0.48979816598667725 2307 38-39 0.4544585377098187 2307 40-41 0.43552606338312216 2307 42-43 0.5342355475989606 2307 44-45 0.6552346365188839 2307 46-47 0.5094120869021452 2307 48-49 0.45444173717672953 2307 50-51 0.49121877708167716 2307 52-53 0.2907444838262876 2307 54-55 0.4659457874453281 2307 56-57 0.3949106725637108 2307 58-59 0.50513055214806 2307 60-61 0.4798284158346604 2307 62-63 0.4031842483363093 2307 64-65 0.2990384634359273 2307 66-67 0.10017346032186936 2307 68-69 0.2001441752380586 2307 70-71 0.23111744921894228 2307 72-73 -0.41447019485098835 2307 74-75 -0.2924670509802425 2307 76-77 -0.2559817440415735 2307 78-79 0.2662387121324876 2307 80-81 0.2559636287834053 2307 82-83 0.3341356740479391 2307 84-85 0.39024246745180946 2307 86-87 0.34931161232946906 2307 88-89 0.4656616940240177 2307 90-91 0.18559525671740928 2307 92-93 0.3166790716001735 2307 94-95 0.2551916199726243 2307 96-97 0.4994270648485539 2307 98-99 0.2786812961034286 2307 100-101 0.286954228983781 2201 1 0.1894503843773876 2201 2 0.18767402734619054 2201 3 0.35894227348067886 2201 4 0.2537729525655621 2201 5 0.16232258660955523 2201 6 0.20447494334172944 2201 7 0.2684607945711761 2201 8 0.1899130261141977 2201 9 0.221984888864192 2201 10-11 0.22165265002836776 2201 12-13 0.2519459842155314 2201 14-15 0.4313266789076948 2201 16-17 0.38426424000522985 2201 18-19 0.37768867517028326 2201 20-21 0.3741059161412821 2201 22-23 0.4798012769564579 2201 24-25 0.3690521304377441 2201 26-27 0.44252689816499924 2201 28-29 0.4912545469776646 2201 30-31 0.36091438651238406 2201 32-33 0.5390513836991957 2201 34-35 0.4290451705224001 2201 36-37 0.4379717796193816 2201 38-39 0.42259832057211355 2201 40-41 0.4512924387457673 2201 42-43 0.29550362229694827 2201 44-45 0.40940844098801676 2201 46-47 0.5511554222923536 2201 48-49 0.3937973337707774 2201 50-51 0.4126987730421732 2201 52-53 0.5699828348212748 2201 54-55 0.5062674099560098 2201 56-57 0.5791850634279214 2201 58-59 0.6273060615216934 2201 60-61 0.5194997464844064 2201 62-63 0.5903680594661509 2201 64-65 0.36287826330271855 2201 66-67 0.17535596158607092 2201 68-69 0.2594410283675188 2201 70-71 0.22450583923516376 2201 72-73 -0.3019889164993401 2201 74-75 -0.1389187184971341 2201 76-77 -0.043087642669558335 2201 78-79 0.2902756564327511 2201 80-81 0.3682219552295507 2201 82-83 0.3835811531179303 2201 84-85 0.31030242127978624 2201 86-87 0.24478058358742416 2201 88-89 0.3828604936108597 2201 90-91 0.36332236135740814 2201 92-93 0.1611217586662903 2201 94-95 0.18615514719429171 2201 96-97 0.13074870740819478 2201 98-99 0.05491722191631965 2201 100-101 0.1297079764693052 1101 1 0.14418614478388747 1101 2 0.13352044276289377 1101 3 0.3011504367007021 1101 4 0.20875764236764383 1101 5 0.18643822852843073 1101 6 0.11653907421063536 1101 7 0.1865651036152869 1101 8 0.14417311642218067 1101 9 0.17703337115761286 1101 10-11 0.16269798707460836 1101 12-13 0.16015448303952695 1101 14-15 0.2591639192999864 1101 16-17 0.1820399622471811 1101 18-19 0.2521367314408067 1101 20-21 0.29140865866369126 1101 22-23 0.25884246194359406 1101 24-25 0.22236025649170443 1101 26-27 0.1803265841950008 1101 28-29 0.3054828381154948 1101 30-31 0.18560533962767778 1101 32-33 0.3111371069396327 1101 34-35 0.38825021900469636 1101 36-37 0.3366724236461849 1101 38-39 0.3766361772553495 1101 40-41 0.2667168390963468 1101 42-43 0.26935632820801914 1101 44-45 0.2274542273552882 1101 46-47 0.36557474420590097 1101 48-49 0.31195780794313777 1101 50-51 0.198556740446989 1101 52-53 0.2935755169904368 1101 54-55 0.3507083797128203 1101 56-57 0.4940342219217513 1101 58-59 0.4558204037378957 1101 60-61 0.385649578405058 1101 62-63 0.42168753780999424 1101 64-65 0.3406226578842393 1101 66-67 0.1342756773489242 1101 68-69 0.12718948625337845 1101 70-71 0.11016854614472038 1101 72-73 -0.1770776712699096 1101 74-75 -0.07199138091836588 1101 76-77 -0.10165920397691153 1101 78-79 0.38736713486534313 1101 80-81 0.0790935825482677 1101 82-83 0.2675211160628166 1101 84-85 0.21153014937954495 1101 86-87 0.1785518431285631 1101 88-89 0.23646470003226838 1101 90-91 0.3076724268140545 1101 92-93 0.30157479629831485 1101 94-95 0.20037393879809073 1101 96-97 0.1982089203087014 1101 98-99 0.24298476707030758 1101 100-101 0.27429469820091157 2304 1 0.09470332887077149 2304 2 0.0996181919076804 2304 3 0.2922099975546786 2304 4 0.21693624765140385 2304 5 0.13777391712835652 2304 6 0.1096790275346251 2304 7 0.04732402666684976 2304 8 0.09760304316247925 2304 9 0.05705001172143653 2304 10-11 0.11996413048227339 2304 12-13 0.1380650039458473 2304 14-15 0.2474166729784315 2304 16-17 0.2613373363352949 2304 18-19 0.28430376560806536 2304 20-21 0.28801357149392715 2304 22-23 0.3471944214601379 2304 24-25 0.2886074422787104 2304 26-27 0.24293702756556002 2304 28-29 0.3120584045644108 2304 30-31 0.25209820556552387 2304 32-33 0.411014782051943 2304 34-35 0.3140437047133915 2304 36-37 0.35629254353036544 2304 38-39 0.4348213189834951 2304 40-41 0.38136559053887 2304 42-43 0.3744163089399919 2304 44-45 0.4520715716655559 2304 46-47 0.4027120520408829 2304 48-49 0.332611362223318 2304 50-51 0.28151629434592707 2304 52-53 0.26237703576388327 2304 54-55 0.3964060147435262 2304 56-57 0.4369258225585213 2304 58-59 0.2895765666711725 2304 60-61 0.3587173346079169 2304 62-63 0.49695871975369244 2304 64-65 0.2504411379258684 2304 66-67 0.08554181560469942 2304 68-69 0.25658468161257275 2304 70-71 0.2272438318163097 2304 72-73 -0.23635517199117118 2304 74-75 -0.20195940063975115 2304 76-77 -0.308052265170911 2304 78-79 0.10277904033869945 2304 80-81 0.03642766633840111 2304 82-83 0.0872488779842584 2304 84-85 0.061013866871199696 2304 86-87 0.10959766778956848 2304 88-89 0.2889927584442553 2304 90-91 0.2396666798948779 2304 92-93 0.30198501453183724 2304 94-95 0.18638079594683532 2304 96-97 -0.06269615506326431 2304 98-99 0.041553929735687944 2304 100-101 -0.013175388711260894 2202 1 0.12239213466856569 2202 2 0.12471848204704372 2202 3 0.26553670919581407 2202 4 0.2214192480497772 2202 5 0.12449530675755227 2202 6 0.10958759831183329 2202 7 0.08983238145735584 2202 8 0.13058969746443694 2202 9 0.14734705576716323 2202 10-11 0.21974161312704155 2202 12-13 0.1070317587368308 2202 14-15 0.06772322210115789 2202 16-17 0.12323750274835987 2202 18-19 0.032698410942821 2202 20-21 0.17139648835922827 2202 22-23 0.18192467259958534 2202 24-25 0.16169343977449557 2202 26-27 0.16739719440607814 2202 28-29 0.2077888676534272 2202 30-31 0.19426966741786345 2202 32-33 0.1821491812283611 2202 34-35 0.18916177605549223 2202 36-37 0.17344834664407216 2202 38-39 0.2260955276902905 2202 40-41 0.3164796366203859 2202 42-43 0.28269721394681113 2202 44-45 0.3826623944276335 2202 46-47 0.32826328416840056 2202 48-49 0.311607724646386 2202 50-51 0.3019137680496371 2202 52-53 0.3424101105820725 2202 54-55 0.4221379976949393 2202 56-57 0.2936461375974986 2202 58-59 0.31990064170725674 2202 60-61 0.3322475356394037 2202 62-63 0.350682352961023 2202 64-65 0.2998607107851612 2202 66-67 0.142848197898779 2202 68-69 0.06854445512932017 2202 70-71 0.15297332124347918 2202 72-73 0.08039409761401473 2202 74-75 0.1446553136468971 2202 76-77 0.19089867355191714 2202 78-79 0.4799309666158713 2202 80-81 0.45384481454220094 2202 82-83 0.5430603628007766 2202 84-85 0.5173218301783997 2202 86-87 0.4038204689316025 2202 88-89 0.4336995407708635 2202 90-91 0.4282603797089912 2202 92-93 0.554063099837677 2202 94-95 0.5878566060898969 2202 96-97 0.6720793951002122 2202 98-99 0.5016581353018239 2202 100-101 0.46832236444527453 1102 1 0.07512677761597075 1102 2 0.06794635128419912 1102 3 0.2960595845703793 1102 4 0.17617618873355667 1102 5 0.04665517714998657 1102 6 0.030542833609132458 1102 7 0.11963997830200412 1102 8 0.14730594850237821 1102 9 0.17628932353856186 1102 10-11 0.138868883064589 1102 12-13 0.1861667794054469 1102 14-15 0.20909734661828594 1102 16-17 0.2314505982120849 1102 18-19 0.18757102028792616 1102 20-21 0.12400773447822644 1102 22-23 0.1364759989110098 1102 24-25 0.13177245887265343 1102 26-27 0.056638457628579886 1102 28-29 0.23504306681223852 1102 30-31 0.12890107897604963 1102 32-33 0.23396174666394387 1102 34-35 0.23739456424279126 1102 36-37 0.17252181274393052 1102 38-39 0.23400420670397182 1102 40-41 0.12395759724171285 1102 42-43 0.23767556379698362 1102 44-45 0.2687586603126775 1102 46-47 0.2843462824264549 1102 48-49 0.19885277974764648 1102 50-51 0.2392443970885907 1102 52-53 0.0873666354114917 1102 54-55 0.2054428721940198 1102 56-57 0.18447125199693915 1102 58-59 0.10071388744717069 1102 60-61 0.1870378146205951 1102 62-63 0.21438216500298068 1102 64-65 0.11309093795942715 1102 66-67 -0.060821440445565145 1102 68-69 -0.11138546675413608 1102 70-71 -0.03135714307833837 1102 72-73 -0.06961174081878241 1102 74-75 -0.24480623054242656 1102 76-77 -0.13880284432778822 1102 78-79 0.10432554651947967 1102 80-81 0.1495920944530269 1102 82-83 0.10948931781720361 1102 84-85 -0.02838604736230721 1102 86-87 0.005844684607257733 1102 88-89 0.0020015891300140254 1102 90-91 0.0011345978666845724 1102 92-93 0.19890601496999238 1102 94-95 0.09318213115398422 1102 96-97 -0.05588334159605779 1102 98-99 -0.12152024546101714 1102 100-101 -0.12678612886675822 2305 1 0.0718428396952504 2305 2 0.07659288581424306 2305 3 0.30379378427119974 2305 4 0.1488731470980511 2305 5 0.07802983434270061 2305 6 0.12076932014615949 2305 7 0.14537779306141374 2305 8 0.12824313685939615 2305 9 0.051346198382745456 2305 10-11 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-0.12373255620720869 1213 56-57 0.040642060472698915 1213 58-59 -0.3783823774225894 1213 60-61 -0.30981271970524915 1213 62-63 -0.08038735938880137 1213 64-65 -0.023922155935018452 1213 66-67 0.2109499303488036 1213 68-69 0.2950704200117755 1213 70-71 0.1949502028942831 1213 72-73 0.7158143594087427 1213 74-75 0.7021236912569968 1213 76-77 0.3817081523489101 1213 78-79 0.004729957954857866 1213 80-81 -0.020045024173576564 1213 82-83 0.005961445983743374 1213 84-85 0.0668408507709124 1213 86-87 0.08426808745221948 1213 88-89 0.17384439745348956 1213 90-91 0.32909598017167596 1213 92-93 0.3073243623221025 1213 94-95 0.4027522686992171 1213 96-97 0.34960427552565676 1213 98-99 0.3109854258726017 1213 100-101 0.6428668896487721 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 10.0 10 19.0 11 17.0 12 42.0 13 112.0 14 298.0 15 759.0 16 1544.0 17 2637.0 18 3525.0 19 4575.0 20 6177.0 21 8318.0 22 11747.0 23 16021.0 24 21793.0 25 29503.0 26 41187.0 27 62717.0 28 114229.0 29 190565.0 30 146465.0 31 74225.0 32 86419.0 33 123423.0 34 191669.0 35 330770.0 36 581195.0 37 707562.0 38 111544.0 39 35.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.33164664693843 19.68040114627184 19.714751599792084 12.27320060699765 2 28.930236708210444 31.51282178186764 28.5048074275505 11.052134082371417 3 36.933611980333914 14.433749654072948 34.87143364021216 13.761204725380974 4 29.187157815106424 13.692346039675583 28.13940314064943 28.981093004568564 5 44.19218975135774 12.190364790098087 29.10259028783222 14.514855170711952 6 38.76969170144526 13.134597515180706 33.3475770467554 14.748133736618636 7 31.849686766103126 28.808595860307513 23.049128263826102 16.292589109763263 8 27.59521968894797 38.023186348899415 18.637155458397782 15.744438503754834 9 42.97564185588383 21.253444457534098 18.92912834747597 16.841785339106103 10-11 31.286772234125888 30.85522538010646 22.89350571041939 14.964496675348258 12-13 23.932261732068085 29.2234120641232 22.46577500555923 24.378551198249486 14-15 23.100119828434124 29.601962565290464 22.686558372619725 24.611359233655687 16-17 29.552887976795528 22.960110863956736 23.67305170746805 23.81394945177969 18-19 20.88972438065987 23.06875112840185 31.340206796412257 24.701317694526022 20-21 27.511273562250487 23.95645048520408 31.33865578846622 17.193620164079213 22-23 20.157735765406738 38.24363860190401 23.997194941134893 17.601430691554363 24-25 20.581614038120637 22.047978775240477 32.33213737259951 25.038269814039378 26-27 19.126005127389185 22.181613302980043 25.315625815259583 33.37675575437118 28-29 26.147254165547707 30.772379705764923 23.65746686112947 19.4228992675579 30-31 17.276067563997376 24.460144672444546 31.339683984745054 26.924103778813024 32-33 17.716240133672486 30.758491681369293 24.01423860148576 27.511029583472457 34-35 18.84634983350191 30.382572665593628 31.643054168168298 19.128023332736166 36-37 17.61965707402458 32.37846078560539 32.181428572424416 17.820453567945616 38-39 17.61032243673081 25.02038974030093 30.204071674293942 27.16521614867432 40-41 26.3276885190392 23.960529693438016 22.573941908829394 27.13783987869339 42-43 19.413475947683054 31.48314125439447 30.42613344756237 18.677249350360107 44-45 17.944034975482694 25.0725882076773 30.14164220047747 26.841734616362544 46-47 26.18876081947231 24.25827382688914 23.7185942083996 25.834371145238944 48-49 18.66595166637533 30.958385623684098 31.64397377271112 18.731688937229446 50-51 25.356829717037115 23.80469299111653 29.86874605805093 20.969731233795418 52-53 17.39254399112161 24.483040709825207 23.199609060755918 34.924806238297265 54-55 25.73785657171101 24.394806683216462 31.358268169393476 18.509068575679056 56-57 18.815287043826185 24.248824822318156 30.157348583730286 26.778539550125373 58-59 17.941019650878417 23.952873170339743 30.38144571941813 27.72466145936371 60-61 24.540039390463857 23.582900962488786 31.885501705578157 19.991557941469207 62-63 17.62875376042646 23.3424235358169 31.820117858936648 27.208704844819998 64-65 26.18187667960299 24.967743430997437 30.363910568041536 18.48646932135804 66-67 19.285435015389 38.805805725019255 22.982246998329583 18.926512261262157 68-69 18.451349068764724 37.292449450776445 24.65097307091926 19.60522840953957 70-71 17.941297466147905 39.525233931584594 23.594702199166072 18.938766403101422 72-73 19.836114151844544 38.9505867259784 23.099171680971672 18.114127441205387 74-75 21.07943017900905 36.84639157125403 23.27875945069237 18.795418799044548 76-77 20.49874484922764 34.78532572648573 23.84054347043567 20.875385953850962 78-79 20.24013058418645 34.846160080665186 24.36629108316724 20.547418251981117 80-81 19.543442371393514 34.92740623214216 24.48137661255341 21.047774783910917 82-83 19.20625247139887 34.00195219815735 24.7226325850488 22.069162745394983 84-85 20.044524953876902 32.974555526005474 24.62961226621698 22.35130725390064 86-87 18.88352173002157 33.28658711094054 25.410824696088554 22.41906646294935 88-89 18.52333306316882 33.5571341297086 25.52303413920793 22.396498667914653 90-91 19.434789490234685 32.37840509995405 24.81740806256271 23.36939734724855 92-93 19.831715454054684 32.396474639047234 24.02533733345117 23.74647257344691 94-95 20.282898656627076 33.35312966182618 23.50789313956987 22.856078541976874 96-97 19.820829604393637 34.06032985732818 22.819437505643524 23.299403032634658 98-99 19.774926368619738 32.32968031037185 24.463312440238514 23.4320808807699 100-101 20.71352446971733 33.24356275207666 23.6844077738883 22.358505004317703 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1351.0 1 819.5 2 383.5 3 596.5 4 567.5 5 353.5 6 305.5 7 344.5 8 395.5 9 392.0 10 345.5 11 332.5 12 312.5 13 311.0 14 315.5 15 305.0 16 315.5 17 316.0 18 327.0 19 347.5 20 361.5 21 400.0 22 425.5 23 478.0 24 570.0 25 680.5 26 844.0 27 1137.5 28 1497.0 29 1951.0 30 2547.5 31 3579.5 32 5966.5 33 10691.5 34 18617.0 35 32733.5 36 53930.0 37 82479.5 38 120971.0 39 153384.0 40 171202.5 41 174787.5 42 190187.5 43 220517.0 44 211352.0 45 180403.0 46 173702.5 47 168487.0 48 145660.0 49 119899.0 50 97885.0 51 85550.0 52 81097.0 53 81672.5 54 73050.0 55 47588.0 56 28848.0 57 23431.5 58 22348.0 59 19669.5 60 15289.5 61 11383.5 62 7666.5 63 4801.5 64 3209.0 65 2232.5 66 1531.5 67 1173.5 68 912.0 69 669.0 70 480.5 71 324.5 72 235.5 73 178.5 74 116.0 75 72.5 76 54.5 77 42.5 78 20.0 79 13.5 80 12.5 81 8.5 82 9.5 83 6.5 84 2.5 85 2.5 86 3.0 87 2.0 88 1.0 89 1.5 90 1.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15722689538399123 2 0.0 3 0.0 4 0.020459363243272636 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.5684350016137455E-4 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.742705557348606E-5 28-29 5.228116672045818E-5 30-31 0.0 32-33 0.0 34-35 0.0 36-37 9.759151121152194E-4 38-39 4.1824933376366545E-4 40-41 2.9625994474926303E-4 42-43 4.356763893371515E-4 44-45 2.265517224553188E-4 46-47 0.0029625994474926304 48-49 0.0036771087260055583 50-51 0.004234774504357112 52-53 0.003973368670754821 54-55 0.00641315645104287 56-57 0.009776578176725679 58-59 0.01751419085135349 60-61 0.004653023838120778 62-63 0.0027534747806107975 64-65 0.010682785066546954 66-67 0.022916578079134168 68-69 0.02560034463745102 70-71 0.025861750471053314 72-73 0.0223763393563561 74-75 0.02535636585942222 76-77 0.02514724119254038 78-79 0.01584119351629883 80-81 0.003084588836507032 82-83 9.933421676887053E-4 84-85 0.005193262560898846 86-87 0.014673580792875262 88-89 0.01671254629497313 90-91 0.023840212024528927 92-93 0.02392734730239636 94-95 0.025269230581554784 96-97 0.026872519694315502 98-99 0.03239689631111058 100-101 0.02366594146879407 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2869102.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.328211263302236 #Duplication Level Percentage of deduplicated Percentage of total 1 76.43630988211537 19.359950048814547 2 10.039671771666399 5.085738552939569 3 3.7775230946482288 2.8703370897976077 4 2.031450848967563 2.058120650946605 5 1.4240675116024288 1.8034541393535715 6 0.8022457790284198 1.2191670345794579 7 0.6303995936569845 1.1176825861910793 8 0.5912857649543294 1.198096861739722 9 0.38926866942754224 0.8873531187700817 >10 3.1656697562290272 15.391467064578833 >50 0.3671997261642595 6.587029382322973 >100 0.30271236719707206 15.746412025740364 >500 0.029171810553564853 4.824660594452229 >1k 0.011781322172853683 5.3820536025309895 >5k 5.520451626280964E-4 1.0246940651233079 >10k+ 6.90056453249357E-4 15.44378318211897 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT 381054 13.281298468998315 TruSeq Adapter, Index 27 (97% over 39bp) GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 20314 0.7080264138395916 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 18664 0.6505171304470876 No Hit GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 11215 0.3908888565132923 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT 10562 0.3681291219343195 TruSeq Adapter, Index 27 (97% over 39bp) TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT 8543 0.2977586715285828 No Hit TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 7688 0.26795840649792163 No Hit GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 7640 0.266285409162867 No Hit TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 5443 0.18971092697296923 No Hit TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 4941 0.17221416317718924 No Hit GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC 4817 0.1678922533949647 No Hit GTGTTTGATCGGGGTTTATCGATTACAGAACAGGCTCCTCTAGAGGGATA 4002 0.13948615281018242 No Hit ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG 3880 0.1352339512502518 TruSeq Adapter, Index 27 (97% over 38bp) TTGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 3747 0.13059835446770454 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3622 0.126241590574333 No Hit AGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCC 3619 0.1261370282408921 No Hit GTGTTTGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAA 3528 0.12296530412651764 No Hit GGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCC 3365 0.11728408400956118 No Hit CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC 3255 0.11345013178339425 TruSeq Adapter, Index 27 (97% over 36bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.007981591452656615 0.0 0.0 2 0.008504403119861196 0.0 0.0 3 0.008957506564771834 0.0 0.0 4 0.009306047676241555 0.0 0.0 5 0.011153315567031079 0.0 0.0 6 0.011815543678823549 0.0 0.0 7 0.013697665680760043 0.0 0.0 8 0.01530095479352076 0.0 0.0 9 0.01742705557348606 0.0 0.0 10-11 0.025879177526626797 0.0 0.0 12-13 0.03339023847879929 0.0 0.0 14-15 0.041824933376366544 0.0 0.0 16-17 0.05099156460802021 0.0 0.0 18-19 0.061238673285230014 0.0 0.0 20-21 0.06756469445840546 0.0 0.0 22-23 0.07371644507584603 0.0 0.0 24-25 0.08042586147163816 0.0 0.0 26-27 0.0860199463107272 0.0 0.0 28-29 0.09433265181928005 0.0 0.0 30-31 0.1754207414027107 0.0 0.0 32-33 0.1871317227480933 0.0 0.0 34-35 0.1961066563684386 0.0 0.0 36-37 0.20590066160073778 0.0 0.0 38-39 0.21808217344660452 0.0 0.0 40-41 0.23293002479521469 0.0 0.0 42-43 0.24880607242266045 0.0 0.0 44-45 0.2648041094391207 0.0 0.0 46-47 0.28259713317964996 0.0 0.0 48-49 0.30242912242227704 0.0 0.0 50-51 0.32416066072241423 0.0 0.0 52-53 0.3470423846904014 0.0 0.0 54-55 0.3727298646057199 0.0 0.0 56-57 0.4029135248589977 0.0 0.0 58-59 0.4352232858922408 0.0 0.0 60-61 0.4693454607051265 0.0 0.0 62-63 0.5073712959664731 0.0 0.0 64-65 0.5478892001748282 0.0 0.0 66-67 0.5911082979970737 0.0 0.0 68-69 0.6385273162125291 0.0 0.0 70-71 0.6911918781556041 0.0 0.0 72-73 0.7475161217691111 0.0 0.0 74-75 0.8102709488892343 0.0 0.0 76-77 0.8819135743518356 0.0 0.0 78-79 0.96646964799439 0.0 0.0 80-81 1.0557658807529324 0.0 0.0 82-83 1.161443545750552 0.0 0.0 84-85 1.2833632265426604 0.0 0.0 86-87 1.4166104934575348 0.0 0.0 88-89 1.5649147363879012 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 48895 0.0 82.79198 3 CGGAAGA 49290 0.0 82.73572 4 GATCGGA 49145 0.0 82.578125 1 GAAGAGC 49395 0.0 82.45846 6 ATCGGAA 49975 0.0 81.01228 2 GGAAGAG 51680 0.0 78.94127 5 AGAGCAC 57475 0.0 70.81664 8 GAGCACA 57555 0.0 70.784225 9 AAGAGCA 57745 0.0 70.37037 7 GCGGAAG 285 0.0 60.00947 3 GAACGGA 305 0.0 59.28992 1 CGTGGCA 115 0.0 57.933178 1 CGAGGTG 280 0.0 56.08582 1 ATCGGAC 145 0.0 55.69844 2 TTGTGTT 23895 0.0 52.536953 1 ATTCGGA 100 0.0 52.258247 2 GGTACGC 55 8.091156E-7 51.91414 1 GTACGGG 140 0.0 47.587967 1 GGCCGCA 40 5.3853454E-4 47.507496 4 GCCGTCT 44590 0.0 45.895226 50-51 >>END_MODULE