FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n01_50330665-1.341000000356c2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n01_50330665-1.341000000356c2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2869102
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT38105413.281298468998315TruSeq Adapter, Index 27 (97% over 39bp)
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC203140.7080264138395916No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA186640.6505171304470876No Hit
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC112150.3908888565132923No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT105620.3681291219343195TruSeq Adapter, Index 27 (97% over 39bp)
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT85430.2977586715285828No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT76880.26795840649792163No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC76400.266285409162867No Hit
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT54430.18971092697296923No Hit
TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT49410.17221416317718924No Hit
GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC48170.1678922533949647No Hit
GTGTTTGATCGGGGTTTATCGATTACAGAACAGGCTCCTCTAGAGGGATA40020.13948615281018242No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG38800.1352339512502518TruSeq Adapter, Index 27 (97% over 38bp)
TTGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT37470.13059835446770454No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36220.126241590574333No Hit
AGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCC36190.1261370282408921No Hit
GTGTTTGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAA35280.12296530412651764No Hit
GGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCC33650.11728408400956118No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC32550.11345013178339425TruSeq Adapter, Index 27 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG488950.082.791983
CGGAAGA492900.082.735724
GATCGGA491450.082.5781251
GAAGAGC493950.082.458466
ATCGGAA499750.081.012282
GGAAGAG516800.078.941275
AGAGCAC574750.070.816648
GAGCACA575550.070.7842259
AAGAGCA577450.070.370377
GCGGAAG2850.060.009473
GAACGGA3050.059.289921
CGTGGCA1150.057.9331781
CGAGGTG2800.056.085821
ATCGGAC1450.055.698442
TTGTGTT238950.052.5369531
ATTCGGA1000.052.2582472
GGTACGC558.091156E-751.914141
GTACGGG1400.047.5879671
GGCCGCA405.3853454E-447.5074964
GCCGTCT445900.045.89522650-51
Produced by FastQC (version 0.11.2)