##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n01_50220371-2.34100000035905.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3425843 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.66458387030579 33.0 33.0 33.0 33.0 33.0 2 32.72785763971087 33.0 33.0 33.0 33.0 33.0 3 32.5665131180851 33.0 33.0 33.0 33.0 33.0 4 36.617000253660194 37.0 37.0 37.0 37.0 37.0 5 36.66816459481652 37.0 37.0 37.0 37.0 37.0 6 36.541754540415305 37.0 37.0 37.0 37.0 37.0 7 36.48973405961686 37.0 37.0 37.0 37.0 37.0 8 36.42525416371971 37.0 37.0 37.0 37.0 37.0 9 36.4167088217411 37.0 37.0 37.0 37.0 37.0 10-11 36.367433650637224 37.0 37.0 37.0 37.0 37.0 12-13 36.36507116642532 37.0 37.0 37.0 37.0 37.0 14-15 38.22894977966007 40.0 37.0 40.0 37.0 40.0 16-17 38.0855625899961 40.0 37.0 40.0 37.0 40.0 18-19 37.88103322306364 40.0 37.0 40.0 33.0 40.0 20-21 37.763499961907186 40.0 37.0 40.0 33.0 40.0 22-23 37.60701190918556 40.0 37.0 40.0 33.0 40.0 24-25 37.511846719187076 40.0 37.0 40.0 33.0 40.0 26-27 37.39762388410677 40.0 37.0 40.0 33.0 40.0 28-29 37.23071416290823 40.0 37.0 40.0 33.0 40.0 30-31 37.152911852644735 40.0 37.0 40.0 33.0 40.0 32-33 37.020456424885786 37.0 37.0 40.0 33.0 40.0 34-35 36.896083241409485 37.0 37.0 40.0 33.0 40.0 36-37 36.78034340744745 37.0 37.0 40.0 33.0 40.0 38-39 36.66230735033683 37.0 37.0 40.0 33.0 40.0 40-41 36.555290770767954 37.0 37.0 40.0 33.0 40.0 42-43 36.395457847893205 37.0 37.0 40.0 33.0 40.0 44-45 36.23623251269834 37.0 37.0 40.0 33.0 40.0 46-47 36.110423916098895 37.0 37.0 40.0 33.0 40.0 48-49 35.91526129481123 37.0 37.0 40.0 30.0 40.0 50-51 35.70896535538844 37.0 37.0 40.0 27.0 40.0 52-53 35.52183141492473 37.0 37.0 40.0 27.0 40.0 54-55 35.36299824597916 37.0 37.0 40.0 27.0 40.0 56-57 35.136434594346554 37.0 33.0 40.0 27.0 40.0 58-59 34.8975345630258 37.0 33.0 40.0 27.0 40.0 60-61 34.605345603987104 37.0 33.0 40.0 27.0 40.0 62-63 34.308260769685006 37.0 33.0 37.0 27.0 40.0 64-65 34.006720097797825 37.0 33.0 37.0 27.0 40.0 66-67 33.70566704311902 37.0 33.0 37.0 24.5 40.0 68-69 33.41942786052951 37.0 33.0 37.0 22.0 40.0 70-71 33.108912171398394 37.0 33.0 37.0 22.0 40.0 72-73 32.79926167077709 37.0 33.0 37.0 22.0 37.0 74-75 32.4741774506304 37.0 33.0 37.0 22.0 37.0 76-77 29.744476323053917 33.0 27.0 35.0 18.5 37.0 78-79 32.11837436800227 35.0 33.0 37.0 22.0 37.0 80-81 32.443501789194656 37.0 33.0 37.0 22.0 37.0 82-83 32.33915827432839 37.0 33.0 37.0 22.0 37.0 84-85 32.143729879039995 37.0 33.0 37.0 22.0 37.0 86-87 31.93982079739206 35.0 33.0 37.0 22.0 37.0 88-89 31.76884098308066 33.0 33.0 37.0 15.0 37.0 90-91 31.597042538143164 33.0 33.0 37.0 15.0 37.0 92-93 31.38485315876997 33.0 33.0 37.0 15.0 37.0 94-95 31.22672872049303 33.0 33.0 37.0 15.0 37.0 96-97 31.044936385000714 33.0 33.0 37.0 15.0 37.0 98-99 30.81373708602525 33.0 30.0 37.0 15.0 37.0 100-101 29.230478308550623 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 0.11247965467720888 2306 2 0.13704837206233123 2306 3 0.30116148580835045 2306 4 0.1815890348109903 2306 5 0.11727160101941081 2306 6 0.07765795712931833 2306 7 0.18477959351401552 2306 8 0.22026337070866475 2306 9 0.16436027632367 2306 10-11 0.11824902369227175 2306 12-13 0.18431275877200903 2306 14-15 0.23559539024333986 2306 16-17 0.39000828932861964 2306 18-19 0.44275481806565864 2306 20-21 0.5742641654004714 2306 22-23 0.6729279778764337 2306 24-25 0.5749159725834403 2306 26-27 0.5270689463582059 2306 28-29 0.5106278128515385 2306 30-31 0.553022137595967 2306 32-33 0.5076795144567967 2306 34-35 0.5599213716755216 2306 36-37 0.6339212908995364 2306 38-39 0.5453037310076141 2306 40-41 0.518107678441929 2306 42-43 0.4908055000015423 2306 44-45 0.5253809701796754 2306 46-47 0.6328537468595741 2306 48-49 0.5944924843511359 2306 50-51 0.5738367295000231 2306 52-53 0.498502956270201 2306 54-55 0.6157987063489543 2306 56-57 0.5705999228072685 2306 58-59 0.5773585577748364 2306 60-61 0.7221457769307378 2306 62-63 0.7374172675986941 2306 64-65 0.5412254172738429 2306 66-67 0.5338692846240036 2306 68-69 0.7377840665562161 2306 70-71 0.624894899322328 2306 72-73 0.6765267420377086 2306 74-75 0.5020128746846311 2306 76-77 0.5214710124363506 2306 78-79 0.646667005944785 2306 80-81 0.7986158891735329 2306 82-83 0.8285691155004216 2306 84-85 0.7085124037406842 2306 86-87 0.7450112789121732 2306 88-89 0.7987680620120337 2306 90-91 0.6218400917791094 2306 92-93 0.7857414558111628 2306 94-95 0.8906689663213427 2306 96-97 0.8244350173160093 2306 98-99 0.6616495083599183 2306 100-101 0.657167705223177 2307 1 0.11061270285440372 2307 2 0.13675016881429514 2307 3 0.3375053076183221 2307 4 0.20935014064631474 2307 5 0.1829853472260865 2307 6 0.21700124960273115 2307 7 0.18380123233863088 2307 8 0.2301443198548654 2307 9 0.17327816999753054 2307 10-11 0.23430185141231874 2307 12-13 0.16953243688447373 2307 14-15 0.3738149173624663 2307 16-17 0.4504389023856561 2307 18-19 0.6149469868425044 2307 20-21 0.6206951334122479 2307 22-23 0.6697922004688124 2307 24-25 0.6336492863575742 2307 26-27 0.7383297820220776 2307 28-29 0.6205282638710585 2307 30-31 0.6870447205588945 2307 32-33 0.690436057816818 2307 34-35 0.5634191547562253 2307 36-37 0.5817131938730711 2307 38-39 0.5396930730102767 2307 40-41 0.6222852583041671 2307 42-43 0.6521200432054144 2307 44-45 0.5362635830042066 2307 46-47 0.7895013404419089 2307 48-49 0.6436381509626514 2307 50-51 0.500279498380813 2307 52-53 0.5151762840705487 2307 54-55 0.5484138869235835 2307 56-57 0.6238331579891963 2307 58-59 0.6580957973758146 2307 60-61 0.5979823023916779 2307 62-63 0.6142065300805015 2307 64-65 0.5010675518450185 2307 66-67 0.6004173636206858 2307 68-69 0.5946968099469885 2307 70-71 0.49860057856363227 2307 72-73 0.47435336660050353 2307 74-75 0.4654686169544746 2307 76-77 0.2948299229179341 2307 78-79 0.6141666353404034 2307 80-81 0.775796805679029 2307 82-83 0.9209171926397843 2307 84-85 0.9028139185237478 2307 86-87 0.9616410628472423 2307 88-89 0.9672244354271662 2307 90-91 0.9678213816749697 2307 92-93 0.9386974513989941 2307 94-95 0.8862113952779538 2307 96-97 1.091729601883113 2307 98-99 1.057177680656043 2307 100-101 0.7205347775998909 2201 1 0.13080678974063886 2201 2 0.09309026522667097 2201 3 0.3018790604062076 2201 4 0.21565850396069663 2201 5 0.1306638283273216 2201 6 0.09418565895675357 2201 7 0.17424517200356604 2201 8 0.15678217114647452 2201 9 0.13841132639282705 2201 10-11 0.14154654547811418 2201 12-13 0.18468029088671756 2201 14-15 0.33847698193972775 2201 16-17 0.3837335925113763 2201 18-19 0.4383143671796219 2201 20-21 0.45296699107532135 2201 22-23 0.567344321387452 2201 24-25 0.4871072785042472 2201 26-27 0.4635273659251382 2201 28-29 0.4804975084336789 2201 30-31 0.4150919072341992 2201 32-33 0.37347824011227004 2201 34-35 0.4929291702189502 2201 36-37 0.3024119040477089 2201 38-39 0.4131386661968648 2201 40-41 0.3762052196162742 2201 42-43 0.3995361908236674 2201 44-45 0.40212454069961723 2201 46-47 0.36944790262423055 2201 48-49 0.26020366190804367 2201 50-51 0.2769516495382689 2201 52-53 0.46480951419910355 2201 54-55 0.5016207281936715 2201 56-57 0.4892199175870289 2201 58-59 0.46584766514902043 2201 60-61 0.3856685851592516 2201 62-63 0.44966494010351 2201 64-65 0.4398699208572978 2201 66-67 0.44855536156139664 2201 68-69 0.37081653380855073 2201 70-71 0.3966095678096906 2201 72-73 0.4562175986415511 2201 74-75 0.47485505807690487 2201 76-77 0.24640882846774304 2201 78-79 0.4218349605423697 2201 80-81 0.4961605281267438 2201 82-83 0.7150848094465303 2201 84-85 0.6349730836342786 2201 86-87 0.758168062465657 2201 88-89 0.7008844240287608 2201 90-91 0.7901134170760891 2201 92-93 0.825530700753788 2201 94-95 0.8445499738640478 2201 96-97 0.733262733951328 2201 98-99 0.6589898351028793 2201 100-101 0.6454879626972065 1101 1 0.14371033351485352 1101 2 0.10211155026313179 1101 3 0.32814598919607363 1101 4 0.19467526207842667 1101 5 0.1551668407970297 1101 6 0.06983156629364373 1101 7 0.12044420309692327 1101 8 0.1476028394511033 1101 9 0.11516979702443564 1101 10-11 0.07078704837983452 1101 12-13 0.1128177376315378 1101 14-15 0.21746997981865235 1101 16-17 0.24603106765896143 1101 18-19 0.21923097087100984 1101 20-21 0.4137981917675049 1101 22-23 0.35224692570432836 1101 24-25 0.3843299469281405 1101 26-27 0.39232476014796447 1101 28-29 0.33872395111522025 1101 30-31 0.3274732885772096 1101 32-33 0.4600561694363634 1101 34-35 0.5513042132672794 1101 36-37 0.4067049015615538 1101 38-39 0.44194415289539535 1101 40-41 0.4491218482543857 1101 42-43 0.3636069677378444 1101 44-45 0.283887081907352 1101 46-47 0.39020266897072986 1101 48-49 0.3041286160608152 1101 50-51 0.26079141669401196 1101 52-53 0.19812760568910903 1101 54-55 0.23461177414311862 1101 56-57 0.36886122620727946 1101 58-59 0.5033675464755945 1101 60-61 0.3907615208538857 1101 62-63 0.3808580569965798 1101 64-65 0.22643231932107 1101 66-67 0.09874790772196462 1101 68-69 0.3509909570827503 1101 70-71 0.330115885416852 1101 72-73 0.381012563904747 1101 74-75 0.22607539428580736 1101 76-77 0.11344534561223085 1101 78-79 0.35931107861220113 1101 80-81 0.52745589618835 1101 82-83 0.5836657421134746 1101 84-85 0.6868218188350923 1101 86-87 0.765384664575059 1101 88-89 0.8778045768487708 1101 90-91 0.8356983174899604 1101 92-93 0.8440020807703661 1101 94-95 0.8397397424192405 1101 96-97 0.7984247325372742 1101 98-99 0.7781881993275661 1101 100-101 0.7388526193876181 2304 1 0.13665599541408113 2304 2 0.08358264517119096 2304 3 0.3170069456214293 2304 4 0.1566158894571359 2304 5 0.06719126556633626 2304 6 0.17479530973341184 2304 7 0.14862483154396955 2304 8 0.127493134978792 2304 9 0.1215130257946555 2304 10-11 0.11071350031151894 2304 12-13 0.12582469515193395 2304 14-15 0.20100095480629676 2304 16-17 0.31276934603990014 2304 18-19 0.5103801636116856 2304 20-21 0.4117917283512398 2304 22-23 0.5695108574378338 2304 24-25 0.5475629237052502 2304 26-27 0.5240444094675709 2304 28-29 0.5606009125083276 2304 30-31 0.537914076205908 2304 32-33 0.5143208222948203 2304 34-35 0.5947336298237715 2304 36-37 0.5805794792360572 2304 38-39 0.5032923560923805 2304 40-41 0.6562808496298658 2304 42-43 0.587090602207617 2304 44-45 0.5594970334317324 2304 46-47 0.6374316795605779 2304 48-49 0.5946969025877991 2304 50-51 0.5192738159034391 2304 52-53 0.6323316306346882 2304 54-55 0.7722538290821532 2304 56-57 0.6307731421294491 2304 58-59 0.40257030277542327 2304 60-61 0.5702915877582555 2304 62-63 0.47867732171621213 2304 64-65 0.39700591412896813 2304 66-67 0.3161320269479617 2304 68-69 0.45195596249119063 2304 70-71 0.3569243733246523 2304 72-73 0.3749756463133096 2304 74-75 0.37044978500198766 2304 76-77 0.25861848237224194 2304 78-79 0.467081700782785 2304 80-81 0.547702480860444 2304 82-83 0.7164779291252188 2304 84-85 0.749409455548367 2304 86-87 0.7359170283959529 2304 88-89 0.9217155780105806 2304 90-91 0.7700450650828721 2304 92-93 0.7954363181807196 2304 94-95 0.9119365360840206 2304 96-97 0.7777716804426724 2304 98-99 0.717810677059493 2304 100-101 0.6496634843369371 2202 1 0.1050376416111547 2202 2 0.0501705903929448 2202 3 0.29081022609282314 2202 4 0.19874885544050613 2202 5 0.1064835126998176 2202 6 0.12134226542635673 2202 7 0.14082380572323672 2202 8 0.043817047416986554 2202 9 0.1545096971891411 2202 10-11 0.0873311290816261 2202 12-13 0.12887556023188296 2202 14-15 0.10173949387524317 2202 16-17 0.09017149556080284 2202 18-19 0.21719734759430054 2202 20-21 0.18017533817885578 2202 22-23 0.3411934295118826 2202 24-25 0.38701372141756707 2202 26-27 0.3105952521419937 2202 28-29 0.34963272684755964 2202 30-31 0.3521412843713918 2202 32-33 0.36614349940800395 2202 34-35 0.25683000934220246 2202 36-37 0.2852845016491372 2202 38-39 0.2892179209004979 2202 40-41 0.34504377245868767 2202 42-43 0.3476760813108086 2202 44-45 0.4207314169046086 2202 46-47 0.44596863886358307 2202 48-49 0.5561475623424954 2202 50-51 0.35194329106527533 2202 52-53 0.315253067287216 2202 54-55 0.3117335651702149 2202 56-57 0.40013208472951334 2202 58-59 0.5309021180713032 2202 60-61 0.3929296171392025 2202 62-63 0.2959302202111118 2202 64-65 0.3633776082436597 2202 66-67 0.33304656420835244 2202 68-69 0.15729488512263146 2202 70-71 0.33549877251664384 2202 72-73 0.23104322692411472 2202 74-75 0.1473546702630344 2202 76-77 -0.017769502323520925 2202 78-79 0.28309298058150745 2202 80-81 0.41995100650117934 2202 82-83 0.45227225795881765 2202 84-85 0.616885540794101 2202 86-87 0.7719877256408765 2202 88-89 0.6289341104535318 2202 90-91 0.7263250198891633 2202 92-93 0.7368889742931231 2202 94-95 0.8432685019249035 2202 96-97 0.8723361622934398 2202 98-99 0.5821362052131711 2202 100-101 0.4916978408224786 1102 1 0.13543258050971474 1102 2 0.03536218512805078 1102 3 0.26583237699654916 1102 4 0.11558200413826825 1102 5 0.03860904199940052 1102 6 0.0024710547332560395 1102 7 0.062046118285934426 1102 8 0.12796353443448538 1102 9 0.055245032074672906 1102 10-11 0.10256448830090648 1102 12-13 0.07468117289112541 1102 14-15 0.11056402521267472 1102 16-17 0.16275801882100893 1102 18-19 0.3225645255769152 1102 20-21 0.2652224080295369 1102 22-23 0.37553373917263855 1102 24-25 0.3817023335458529 1102 26-27 0.4260688581970342 1102 28-29 0.4119095261620984 1102 30-31 0.44161239435850774 1102 32-33 0.41294334216073025 1102 34-35 0.38850128861933797 1102 36-37 0.3334950150624323 1102 38-39 0.45796184214522384 1102 40-41 0.43214830385812064 1102 42-43 0.3030999138589223 1102 44-45 0.4252469103752361 1102 46-47 0.39391303142087963 1102 48-49 0.4197843216226502 1102 50-51 0.35730135948501385 1102 52-53 0.35174080139194785 1102 54-55 0.24244085237934598 1102 56-57 0.3874537551991821 1102 58-59 0.4410369360249149 1102 60-61 0.3435099404118418 1102 62-63 0.41726554381700964 1102 64-65 0.28871055143969926 1102 66-67 0.3058731792986791 1102 68-69 0.3049717498495639 1102 70-71 0.30729948866659385 1102 72-73 0.24272171038698787 1102 74-75 0.28403251283715036 1102 76-77 0.1764831623726657 1102 78-79 0.2788419505987676 1102 80-81 0.40480284077247575 1102 82-83 0.3505991627308589 1102 84-85 0.45899386327405267 1102 86-87 0.6136946431019155 1102 88-89 0.7280122322285969 1102 90-91 0.7508887058090785 1102 92-93 0.7114374636974787 1102 94-95 0.5490813092310631 1102 96-97 0.6641083073248346 1102 98-99 0.6272006404661781 1102 100-101 0.5036616539185736 2305 1 0.12487285525372727 2305 2 0.07412018909442253 2305 3 0.2526582403586062 2305 4 0.1356416460074712 2305 5 0.039142630727113215 2305 6 0.028127526539257985 2305 7 0.10011708883766346 2305 8 0.005200199348891488 2305 9 0.05295766593646789 2305 10-11 0.015362338688213129 2305 12-13 0.052226829458298596 2305 14-15 0.043707113141799425 2305 16-17 0.09115597068120707 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1213 56-57 -0.12813954837282893 1213 58-59 -0.5465443904870213 1213 60-61 -0.44853755301053866 1213 62-63 -0.25138669103625233 1213 64-65 -0.15266077325338046 1213 66-67 -0.043151821615715846 1213 68-69 -0.06370754752588681 1213 70-71 -0.2447441695529946 1213 72-73 -0.12026489416132335 1213 74-75 -0.0959088736395799 1213 76-77 0.011896639893727468 1213 78-79 -0.4163080827698593 1213 80-81 -0.4485996069388918 1213 82-83 -0.626933417086974 1213 84-85 -0.5415794610400937 1213 86-87 -0.5846849154163181 1213 88-89 -0.5600015916405852 1213 90-91 -0.505261854713627 1213 92-93 -0.6331462221224129 1213 94-95 -0.6119205157814775 1213 96-97 -0.6593438831404264 1213 98-99 -0.8629741363565024 1213 100-101 -0.535942620966356 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 3.0 8 18.0 9 70.0 10 133.0 11 128.0 12 171.0 13 535.0 14 1533.0 15 3964.0 16 7498.0 17 11618.0 18 14253.0 19 16038.0 20 18106.0 21 21069.0 22 24076.0 23 27390.0 24 31302.0 25 35979.0 26 41637.0 27 48558.0 28 57093.0 29 67154.0 30 80010.0 31 96141.0 32 120643.0 33 159669.0 34 231950.0 35 381670.0 36 731805.0 37 944640.0 38 250930.0 39 58.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 32.53243808497393 31.49154228302951 20.514506608080215 15.461513023916334 2 30.07040894752036 24.9626442309236 31.52222679206257 13.444720029493471 3 33.214715327001265 22.99320196518054 26.364839252703643 17.427243455114553 4 29.251940802246924 23.144581373553706 30.17616849579432 17.42730932840505 5 29.121387057141845 21.44240118417569 31.379313062507535 18.056898696174926 6 27.34667058589667 22.181635293853223 32.32261373332052 18.14908038692958 7 30.70747842209932 22.262695634330004 26.773994021325553 20.255831922245125 8 27.362520699284822 26.91682018119336 26.240928145276943 19.47973097424488 9 24.710180822647157 24.690185744063577 30.812532856876395 19.787100576412872 10-11 19.763269738224785 30.164456016626577 34.76665576909605 15.305618476052588 12-13 19.777196379751313 29.740163148212908 34.60917053384248 15.873469938193296 14-15 20.05380865381163 29.606902593026007 34.685273084610124 15.654015668552237 16-17 19.89840164887883 29.4952220519154 34.58286033539774 16.023515963808034 18-19 19.17001158546962 29.78247981591684 34.28791103386816 16.75959756474538 20-21 19.231937949287225 29.972841721001224 33.872597197244595 16.922623132466956 22-23 19.139449764627276 29.631028041857142 33.9638885961791 17.26563359733648 24-25 19.112259376743186 29.091905262441976 34.26864570267814 17.5271896581367 26-27 19.127394337685644 29.486479678140533 34.06907438548702 17.317051598686806 28-29 18.972584975862127 29.873311381542717 33.920493379653834 17.233610262941312 30-31 18.294825536371633 30.936969382426454 33.734178711633895 17.034026369568018 32-33 18.102303579002307 31.364630544949083 33.72435631171656 16.80870956433205 34-35 18.46091604314617 30.749234567958894 34.22227755329126 16.567571835603676 36-37 18.590940576559714 30.37887759453769 34.571427487219786 16.45875434168281 38-39 18.876295474325055 30.247980009460505 33.898623701971545 16.977100814242895 40-41 18.699006037798966 30.09863728093111 33.43246733022276 17.76988935104717 42-43 18.916240139049776 29.759457835466424 33.41443305529313 17.909868970190672 44-45 18.543830041746094 29.62346783457846 33.63200065852664 18.200701465148803 46-47 18.696826091151124 29.90871868567518 33.50490267982416 17.889552543349538 48-49 18.56780220842004 30.606703959211924 32.96107927502264 17.86441455734539 50-51 19.15066901228061 30.471983044556495 32.05873161991421 18.318616323248683 52-53 19.087020831089266 30.4108039911294 32.07529737743881 18.426877800342528 54-55 19.43023452688856 30.508535681132177 32.057598332564616 18.003631459414645 56-57 19.41240935256986 30.542312546179428 31.939000384469757 18.10627771678095 58-59 19.15386609631214 31.15671086213571 31.825321335334944 17.864101706217202 60-61 19.037075428998957 31.062313640932736 31.651802701275088 18.248808228793216 62-63 18.60652378375254 30.939618336699027 32.00360680985316 18.450251069695266 64-65 18.545708047162517 30.53083594696151 32.34772292130623 18.57573308456974 66-67 18.832085119328987 30.464285391034874 31.988200384989668 18.715429104646475 68-69 19.179052107392344 30.536893107078328 31.9315681619805 18.35248662354883 70-71 18.73361288988913 30.818876666156687 32.08428273947517 18.36322770447902 72-73 18.74897811889791 30.671440116599552 31.86647167228799 18.71311009221455 74-75 19.028859781125345 30.352668370621206 31.436910178004275 19.181561670249174 76-77 19.04855058044958 30.04815072345794 31.134275598256277 19.769023097836204 78-79 18.796608232258052 30.206958907316995 31.342277676350943 19.654155184074007 80-81 18.467483078029638 30.59505085640291 30.95936800512826 19.978098060439194 82-83 18.085235565415378 30.859697005079106 30.727304571195052 20.327762858310468 84-85 18.059927596233184 30.967855594141902 31.184704919547396 19.787511890077518 86-87 18.261652222134153 30.68181901119727 31.512348611942535 19.544180154726043 88-89 18.64963014583672 30.61893824981337 31.343538273928267 19.38789333042164 90-91 18.502832755940364 30.614377165223633 30.93761601204547 19.945174066790532 92-93 18.119639453621346 30.78406165912998 30.830937243067492 20.26536164418118 94-95 17.913309097619166 30.62444579855082 30.81233049136446 20.649914612465558 96-97 17.940850271327623 31.1043751844022 30.543500524173055 20.41127402009712 98-99 18.453984319269964 30.84306626569876 30.257148555222003 20.445800859809275 100-101 18.617138607743048 30.846769866992073 29.78753564028722 20.748555884977655 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 170440.0 1 93815.0 2 14471.0 3 12154.5 4 11097.0 5 8955.5 6 8407.5 7 8790.5 8 8909.5 9 8577.0 10 7840.0 11 6909.0 12 6155.0 13 5672.5 14 5464.0 15 5280.5 16 5218.0 17 5231.0 18 5271.0 19 5450.0 20 5631.0 21 5785.5 22 6206.5 23 7017.5 24 7985.5 25 9179.5 26 11110.0 27 13908.0 28 17044.0 29 20410.0 30 24942.0 31 31086.5 32 40048.5 33 54974.5 34 75744.0 35 98176.5 36 127346.5 37 166370.5 38 195531.5 39 199381.5 40 198043.5 41 197648.5 42 189509.5 43 183175.0 44 172967.5 45 160022.0 46 155741.0 47 144742.0 48 120293.0 49 96907.0 50 78013.5 51 62730.5 52 50843.0 53 40092.0 54 31701.5 55 26259.0 56 20934.5 57 15487.5 58 11276.5 59 8149.5 60 5917.5 61 4518.5 62 3449.0 63 2603.5 64 2058.5 65 1548.5 66 1042.0 67 744.5 68 592.5 69 452.5 70 341.0 71 276.0 72 211.0 73 174.0 74 142.5 75 103.0 76 74.0 77 62.5 78 46.5 79 36.0 80 31.5 81 26.5 82 23.5 83 18.0 84 14.0 85 11.0 86 5.5 87 2.5 88 1.5 89 2.5 90 2.5 91 1.5 92 1.0 93 0.5 94 0.5 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16673268448087084 2 0.0 3 0.0 4 0.020812395664366407 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 8.756968722734814E-5 12-13 2.918989574244938E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 7.297473935612344E-5 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.001167595829697975 38-39 6.56772654205111E-4 40-41 2.481141138108197E-4 42-43 5.692029669777628E-4 44-45 3.940635925230665E-4 46-47 0.003342243062510454 48-49 0.003969825820973116 50-51 0.004626598475178226 52-53 0.004261724778397609 54-55 0.006509346750566211 56-57 0.010099703926887485 58-59 0.017659886924181873 60-61 0.0048017378496329225 62-63 0.002948179469987387 64-65 0.010756476581092595 66-67 0.02381895492583869 68-69 0.025906032471423823 70-71 0.02612495668949219 72-73 0.02297244794930766 74-75 0.02536601940018851 76-77 0.025351424452317285 78-79 0.016039847710475934 80-81 0.0034735975933514757 82-83 0.0012551655169253232 84-85 0.0056190549304215055 86-87 0.014551163027611013 88-89 0.01675500015616594 90-91 0.023979499352422162 92-93 0.02390652461306604 94-95 0.02539520929593096 96-97 0.02698605861389445 98-99 0.0329845821889678 100-101 0.023979499352422162 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3425843.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.706891330746885 #Duplication Level Percentage of deduplicated Percentage of total 1 81.6967494612818 35.70710950779497 2 8.817916475908511 7.708074343522715 3 2.9389905375235283 3.853624201369027 4 1.6783607638773563 2.934237260823078 5 1.044013100420663 2.2815283563981024 6 0.6615640652886539 1.7348945225938999 7 0.5254633867319597 1.6076459799525988 8 0.392396095658101 1.3720330809230386 9 0.35199368802601905 1.3846094884495832 >10 1.595728558148233 13.177962844633399 >50 0.1616854455767002 4.920451851564126 >100 0.1226939075745865 10.790387520119094 >500 0.010212990421483416 2.94249297995822 >1k 0.0021056589915050003 1.2685583716186717 >5k 0.0 0.0 >10k+ 1.2586457087375338E-4 8.316389690279399 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 189499 5.531456053298414 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 112852 3.294138114326897 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT 4063 0.1185985464015718 TruSeq Adapter, Index 19 (97% over 38bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 8.756968722734814E-5 0.0 0.0 2 1.7513937445469627E-4 0.0 0.0 3 2.0432927019714564E-4 0.0 0.0 4 2.6270906168204437E-4 0.0 0.0 5 3.2108885316694315E-4 0.0 0.0 6 4.3784843613674067E-4 0.0 0.0 7 4.962282276216395E-4 0.0 0.0 8 5.837979148489875E-4 0.0 0.0 9 7.297473935612344E-4 0.0 0.0 10-11 0.0022768118679110513 0.0 0.0 12-13 0.008508854608923994 0.0 0.0 14-15 0.02145457337070029 0.0 0.0 16-17 0.0537385980618493 0.0 0.0 18-19 0.09041570206223695 0.0 0.0 20-21 0.10118677359120076 0.0 0.0 22-23 0.10911183028527577 0.0 0.0 24-25 0.11816069796543507 0.0 0.0 26-27 0.128742035172073 0.0 0.0 28-29 0.1398779803978174 0.0 0.0 30-31 0.15289667389894984 0.0 0.0 32-33 0.16665970974151473 0.0 0.0 34-35 0.182203329224369 0.0 0.0 36-37 0.19876859505820904 0.0 0.0 38-39 0.21793176161312705 0.0 0.0 40-41 0.23750358670843935 0.0 0.0 42-43 0.2573527158133049 0.0 0.0 44-45 0.2793181123594981 0.0 0.0 46-47 0.30259705421410144 0.0 0.0 48-49 0.3282695675195857 0.0 0.0 50-51 0.3554161705600636 0.0 0.0 52-53 0.382723318027125 0.0 0.0 54-55 0.4133143287652119 0.0 0.0 56-57 0.4428836931523132 0.0 0.0 58-59 0.47468608456371175 0.0 0.0 60-61 0.5107793906492504 0.0 0.0 62-63 0.5473689249624107 0.0 0.0 64-65 0.587986664888029 0.0 0.0 66-67 0.6319028630325441 0.0 0.0 68-69 0.678796430542789 0.0 0.0 70-71 0.731148508556872 0.0 0.0 72-73 0.7883898941078152 0.0 0.0 74-75 0.8531768677081817 0.0 0.0 76-77 0.9242980486846595 0.0 0.0 78-79 1.0063362506688134 0.0 0.0 80-81 1.1033050843252301 0.0 0.0 82-83 1.2144164224688638 0.0 0.0 84-85 1.3427935839441563 0.0 0.0 86-87 1.486451655840621 1.4594947871224688E-5 0.0 88-89 1.6517832253258542 2.9189895742449375E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGGAG 155 0.0 55.248703 1 CGGAGCG 80 6.91216E-11 53.522182 1 CGTGCGG 65 4.3679393E-8 51.234913 1 CGAGGCG 170 0.0 44.77673 1 CGCGAGG 75 1.346998E-7 44.40359 1 CGGACGG 60 8.217168E-5 39.64606 1 CGTGGCT 160 0.0 38.654907 1 GTCGACC 50 0.0016037384 38.06022 1 CGGGGTG 635 0.0 36.711628 1 CGACGGG 105 4.5976776E-8 36.247826 1 CGGGGCG 210 0.0 36.247826 1 GGCCCGC 135 3.0377123E-10 35.240944 1 GTCGCGC 55 0.002560376 34.6002 1 CGCGGGG 260 0.0 32.93673 1 TCGAGCT 160 5.2750693E-11 32.650715 8 AGTCGCC 75 3.0512412E-4 31.71685 1 AACGGTG 60 0.003921519 31.716848 1 CGCAGCC 195 0.0 31.716848 1 CGATGCG 60 0.003921519 31.716848 1 GGACGGG 380 0.0 31.299522 1 >>END_MODULE