FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n01_50220371-2.34100000035905.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n01_50220371-2.34100000035905.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3425843
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1894995.531456053298414No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1128523.294138114326897No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT40630.1185985464015718TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAG1550.055.2487031
CGGAGCG806.91216E-1153.5221821
CGTGCGG654.3679393E-851.2349131
CGAGGCG1700.044.776731
CGCGAGG751.346998E-744.403591
CGGACGG608.217168E-539.646061
CGTGGCT1600.038.6549071
GTCGACC500.001603738438.060221
CGGGGTG6350.036.7116281
CGACGGG1054.5976776E-836.2478261
CGGGGCG2100.036.2478261
GGCCCGC1353.0377123E-1035.2409441
GTCGCGC550.00256037634.60021
CGCGGGG2600.032.936731
TCGAGCT1605.2750693E-1132.6507158
AGTCGCC753.0512412E-431.716851
AACGGTG600.00392151931.7168481
CGCAGCC1950.031.7168481
CGATGCG600.00392151931.7168481
GGACGGG3800.031.2995221
Produced by FastQC (version 0.11.2)