##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n01_50110001-2.341000000358f7.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6052628 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67669134795662 33.0 33.0 33.0 33.0 33.0 2 32.72516533314124 33.0 33.0 33.0 33.0 33.0 3 32.55920304370267 33.0 33.0 33.0 33.0 33.0 4 36.605563071115554 37.0 37.0 37.0 37.0 37.0 5 36.658504702420174 37.0 37.0 37.0 37.0 37.0 6 36.535007768526334 37.0 37.0 37.0 37.0 37.0 7 36.471269504750666 37.0 37.0 37.0 37.0 37.0 8 36.399992366952006 37.0 37.0 37.0 37.0 37.0 9 36.392574927783436 37.0 37.0 37.0 37.0 37.0 10-11 36.33694339054044 37.0 37.0 37.0 37.0 37.0 12-13 36.34910661947174 37.0 37.0 37.0 37.0 37.0 14-15 38.18012390650805 40.0 37.0 40.0 37.0 40.0 16-17 38.17592399532897 40.0 37.0 40.0 37.0 40.0 18-19 38.167388777238585 40.0 37.0 40.0 37.0 40.0 20-21 38.08615893790268 40.0 37.0 40.0 37.0 40.0 22-23 37.98037744926667 40.0 37.0 40.0 35.0 40.0 24-25 37.931138011455516 40.0 37.0 40.0 33.0 40.0 26-27 37.859707386609585 40.0 37.0 40.0 33.0 40.0 28-29 37.72950022700883 40.0 37.0 40.0 33.0 40.0 30-31 37.68569289240971 40.0 37.0 40.0 33.0 40.0 32-33 37.56452569032823 40.0 37.0 40.0 33.0 40.0 34-35 37.446439298764105 40.0 37.0 40.0 33.0 40.0 36-37 37.34492215612788 40.0 37.0 40.0 33.0 40.0 38-39 37.27733985964444 40.0 37.0 40.0 33.0 40.0 40-41 37.17016509192371 40.0 37.0 40.0 33.0 40.0 42-43 37.0187820893668 40.0 37.0 40.0 33.0 40.0 44-45 36.883718939938156 38.5 37.0 40.0 33.0 40.0 46-47 36.78838902704743 37.0 37.0 40.0 33.0 40.0 48-49 36.601875251543625 37.0 37.0 40.0 33.0 40.0 50-51 36.41573924582842 37.0 37.0 40.0 33.0 40.0 52-53 36.27040675554487 37.0 37.0 40.0 33.0 40.0 54-55 36.12622632681209 37.0 37.0 40.0 33.0 40.0 56-57 35.94296196627316 37.0 37.0 40.0 30.0 40.0 58-59 35.70254111106779 37.0 37.0 40.0 27.0 40.0 60-61 35.405384652749184 37.0 33.0 40.0 27.0 40.0 62-63 35.09860675395878 37.0 33.0 38.5 27.0 40.0 64-65 34.79546215296892 37.0 33.0 37.0 27.0 40.0 66-67 34.49369538322857 37.0 33.0 37.0 27.0 40.0 68-69 34.24512583624832 37.0 33.0 37.0 27.0 40.0 70-71 33.96889549465125 37.0 33.0 37.0 27.0 40.0 72-73 33.661678282557595 37.0 33.0 37.0 27.0 37.0 74-75 33.34777818825145 37.0 33.0 37.0 27.0 37.0 76-77 30.662314948151447 33.0 30.0 35.0 24.5 37.0 78-79 33.05821785181577 35.0 33.0 37.0 27.0 37.0 80-81 33.44013963851735 37.0 33.0 37.0 27.0 37.0 82-83 33.39285571490599 37.0 33.0 37.0 27.0 37.0 84-85 33.26510847849893 37.0 33.0 37.0 27.0 37.0 86-87 33.132430739176435 37.0 33.0 37.0 27.0 37.0 88-89 33.02753506410769 37.0 33.0 37.0 27.0 37.0 90-91 32.907898189018056 37.0 33.0 37.0 27.0 37.0 92-93 32.75026451319989 35.0 33.0 37.0 27.0 37.0 94-95 32.65170435057301 33.0 33.0 37.0 27.0 37.0 96-97 32.525233171442224 33.0 33.0 37.0 27.0 37.0 98-99 32.34420230352832 33.0 33.0 37.0 22.0 37.0 100-101 30.649154879500273 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 0.1618456351235693 2306 2 0.13176409890154872 2306 3 0.3428117692685717 2306 4 0.24158307697783243 2306 5 0.18450265522560017 2306 6 0.16399769480083393 2306 7 0.21700747667839693 2306 8 0.20625829420492892 2306 9 0.20965244685117312 2306 10-11 0.1543956446797523 2306 12-13 0.24155664261994048 2306 14-15 0.4047264602992371 2306 16-17 0.4141692071611516 2306 18-19 0.3495567142308289 2306 20-21 0.4346104948422962 2306 22-23 0.4338386708211104 2306 24-25 0.4144731357302689 2306 26-27 0.3795217836899951 2306 28-29 0.48370076674085993 2306 30-31 0.39310043580442766 2306 32-33 0.45485075894712423 2306 34-35 0.4391017618804085 2306 36-37 0.4042317876414714 2306 38-39 0.4452846360684717 2306 40-41 0.4511463551648518 2306 42-43 0.46361558408226955 2306 44-45 0.4828173985241335 2306 46-47 0.5062699376797823 2306 48-49 0.504475480656879 2306 50-51 0.5490877852364946 2306 52-53 0.483171903101443 2306 54-55 0.5187088764577581 2306 56-57 0.5417423266536261 2306 58-59 0.48351884837934733 2306 60-61 0.546652630264461 2306 62-63 0.5320287001707626 2306 64-65 0.4468681010771647 2306 66-67 0.353707293539685 2306 68-69 0.4611037598237644 2306 70-71 0.44730042555232785 2306 72-73 0.5700990131403216 2306 74-75 0.41926654604980484 2306 76-77 0.26287244452078795 2306 78-79 0.4038515334674102 2306 80-81 0.5379201682963171 2306 82-83 0.4107180904387562 2306 84-85 0.5328106141677011 2306 86-87 0.5007888695116165 2306 88-89 0.4867803718334045 2306 90-91 0.3943166933765596 2306 92-93 0.4748612552835425 2306 94-95 0.4732297129517917 2306 96-97 0.5242425367566454 2306 98-99 0.39684248752924844 2306 100-101 0.38181106828073297 2307 1 0.13427240694363718 2307 2 0.13107075468730756 2307 3 0.29596161215066275 2307 4 0.18634391151577034 2307 5 0.12970862664312932 2307 6 0.18360459964538478 2307 7 0.1844814228758196 2307 8 0.26443537258578687 2307 9 0.20639430936799386 2307 10-11 0.20603038109169347 2307 12-13 0.19818188764620004 2307 14-15 0.3542485898287424 2307 16-17 0.30631072891375055 2307 18-19 0.34105844328649937 2307 20-21 0.3780240506444912 2307 22-23 0.38717150596392 2307 24-25 0.38464309020861975 2307 26-27 0.4024157470716574 2307 28-29 0.3335563065520901 2307 30-31 0.44807583935183004 2307 32-33 0.35936407017139516 2307 34-35 0.3742525773280718 2307 36-37 0.3610533676186307 2307 38-39 0.3322074773127639 2307 40-41 0.4054872650373724 2307 42-43 0.41594916827086337 2307 44-45 0.3958439644624505 2307 46-47 0.3402984782886733 2307 48-49 0.3235446298979383 2307 50-51 0.28902222387098675 2307 52-53 0.3530938262449794 2307 54-55 0.3883745533552627 2307 56-57 0.45607990109144936 2307 58-59 0.32784682797212383 2307 60-61 0.32008195422483254 2307 62-63 0.41043165103156554 2307 64-65 0.322995683262306 2307 66-67 0.1354043286607407 2307 68-69 0.22302740377000418 2307 70-71 0.18222448186143225 2307 72-73 0.22641329024217072 2307 74-75 0.042481437995185445 2307 76-77 -0.24561969021307917 2307 78-79 0.09085868338934944 2307 80-81 0.25442562783497635 2307 82-83 0.35847848540522165 2307 84-85 0.2903028914614296 2307 86-87 0.34091297413029054 2307 88-89 0.2842375024357864 2307 90-91 0.2452407900229261 2307 92-93 0.23699391092554833 2307 94-95 0.3729421292216557 2307 96-97 0.34147339150650424 2307 98-99 0.16633633626366873 2307 100-101 0.14161282016406318 2201 1 0.1387294411384019 2201 2 0.11025272843466638 2201 3 0.28717103574796 2201 4 0.2117077029162644 2201 5 0.14414683568946884 2201 6 0.11236126619706255 2201 7 0.1668107460899435 2201 8 0.19762841056311942 2201 9 0.13590681913269265 2201 10-11 0.2545561453839511 2201 12-13 0.24704794880121028 2201 14-15 0.3491370216695131 2201 16-17 0.3723334749658136 2201 18-19 0.3789905071856552 2201 20-21 0.3235947702053821 2201 22-23 0.3161904663235191 2201 24-25 0.3042899207491132 2201 26-27 0.35711344084668895 2201 28-29 0.42562019953498265 2201 30-31 0.47697994731740323 2201 32-33 0.5097900646574729 2201 34-35 0.4116374082738048 2201 36-37 0.33499942210692524 2201 38-39 0.33100544605241566 2201 40-41 0.4556230731800852 2201 42-43 0.44651440426765987 2201 44-45 0.39788797052136005 2201 46-47 0.42148329116218974 2201 48-49 0.44214046564945875 2201 50-51 0.41965749299738775 2201 52-53 0.42698855864427543 2201 54-55 0.4748450920133891 2201 56-57 0.4909429269781995 2201 58-59 0.5337508155296931 2201 60-61 0.42642194823923774 2201 62-63 0.38856861294100753 2201 64-65 0.29519284802199053 2201 66-67 0.3111693764985688 2201 68-69 0.4340353816695739 2201 70-71 0.3353548319050361 2201 72-73 0.41685961002014693 2201 74-75 0.42099997384281806 2201 76-77 0.1349573244432598 2201 78-79 0.3213093137117511 2201 80-81 0.35443111734677046 2201 82-83 0.3568253977905371 2201 84-85 0.3840514389546783 2201 86-87 0.4624116425712188 2201 88-89 0.46192914422232434 2201 90-91 0.4489353371974616 2201 92-93 0.32878306397626744 2201 94-95 0.5043518556761128 2201 96-97 0.5184657593668831 2201 98-99 0.3793339537891143 2201 100-101 0.28627288875476253 1101 1 0.1279399238424901 1101 2 0.08863458434323945 1101 3 0.3047263737466963 1101 4 0.19175093527713472 1101 5 0.1386877877333248 1101 6 0.14768880355164526 1101 7 0.154275906009957 1101 8 0.13106518770471354 1101 9 0.18976064099604173 1101 10-11 0.11757320027581386 1101 12-13 0.14788090341468063 1101 14-15 0.26489826864727206 1101 16-17 0.20988550011174567 1101 18-19 0.20595762399202044 1101 20-21 0.270104061227471 1101 22-23 0.23637971866484264 1101 24-25 0.29056346821676726 1101 26-27 0.2499997828725924 1101 28-29 0.24956342792354036 1101 30-31 0.2526662873305625 1101 32-33 0.33430596493953857 1101 34-35 0.4047627068877162 1101 36-37 0.3037196770517667 1101 38-39 0.3971599419123564 1101 40-41 0.34898746338341624 1101 42-43 0.2680261140650302 1101 44-45 0.3203278395634399 1101 46-47 0.35295084772542396 1101 48-49 0.25893350795493575 1101 50-51 0.21077465789903727 1101 52-53 0.18494123606793522 1101 54-55 0.2962382051168717 1101 56-57 0.3548317227979325 1101 58-59 0.362818799744673 1101 60-61 0.3675765105771447 1101 62-63 0.3150745812920164 1101 64-65 0.2637095576579682 1101 66-67 0.14710267336515415 1101 68-69 0.2867073193640479 1101 70-71 0.20394427457380715 1101 72-73 0.3296704829846817 1101 74-75 0.19338996804552977 1101 76-77 -0.028489497212394355 1101 78-79 0.14349897604827078 1101 80-81 0.3031266743971628 1101 82-83 0.3244775461191267 1101 84-85 0.28752307796057863 1101 86-87 0.4000590932598129 1101 88-89 0.3674368908691861 1101 90-91 0.4312711594249876 1101 92-93 0.39157989065764554 1101 94-95 0.2930105835234471 1101 96-97 0.34109309218614925 1101 98-99 0.10312071963354441 1101 100-101 0.12095367935084411 2304 1 0.12892408875911343 2304 2 0.08262594278816238 2304 3 0.2877431357972142 2304 4 0.20087163522054396 2304 5 0.10611206517825167 2304 6 0.11529674184644279 2304 7 0.08869914700999004 2304 8 0.13615526020592483 2304 9 0.0869677250163221 2304 10-11 0.11346532557193001 2304 12-13 0.08890863397108006 2304 14-15 0.21613676695748296 2304 16-17 0.2398655141796766 2304 18-19 0.21953871020049576 2304 20-21 0.21440197630042235 2304 22-23 0.265691290463522 2304 24-25 0.22529437424090304 2304 26-27 0.22881197873986991 2304 28-29 0.24979902828872724 2304 30-31 0.2373003429926257 2304 32-33 0.24825145621510103 2304 34-35 0.248164387276681 2304 36-37 0.29370214093980707 2304 38-39 0.29063349024829677 2304 40-41 0.30014387229213924 2304 42-43 0.33542015083366294 2304 44-45 0.3344966404914942 2304 46-47 0.4055366752210219 2304 48-49 0.31637844166566254 2304 50-51 0.28829911316398693 2304 52-53 0.21796841250196763 2304 54-55 0.3871434787140373 2304 56-57 0.37776812575535956 2304 58-59 0.24995461124985496 2304 60-61 0.23174550443420827 2304 62-63 0.18787985305068133 2304 64-65 0.18115763724640033 2304 66-67 0.1455845679613077 2304 68-69 0.2104065257902903 2304 70-71 0.18569903502142182 2304 72-73 0.1110073554178328 2304 74-75 0.04189036491184339 2304 76-77 -0.16337369479076358 2304 78-79 0.11124340215899764 2304 80-81 0.1519879998255007 2304 82-83 0.2298407796153512 2304 84-85 0.33202291026365316 2304 86-87 0.2730319673656112 2304 88-89 0.409991544819988 2304 90-91 0.301612992554702 2304 92-93 0.38738659758116256 2304 94-95 0.3130949661320912 2304 96-97 0.26517607602164617 2304 98-99 0.17318326721797206 2304 100-101 0.1768783135631189 2202 1 0.1032311039649727 2202 2 0.08067860996990106 2202 3 0.310372049862373 2202 4 0.1740724557361375 2202 5 0.10895634490479011 2202 6 0.08176817618551269 2202 7 0.15235918241271662 2202 8 0.13332876337788946 2202 9 0.1269000319179412 2202 10-11 0.143053784316173 2202 12-13 0.14958349707790575 2202 14-15 0.07590054821179848 2202 16-17 0.06931588485119278 2202 18-19 0.10455765144748597 2202 20-21 0.16056713289677305 2202 22-23 0.15272830953803407 2202 24-25 0.237799547447743 2202 26-27 0.20646031622864314 2202 28-29 0.27215923080853344 2202 30-31 0.16218432539472616 2202 32-33 0.19554252332396516 2202 34-35 0.19139076174713665 2202 36-37 0.16069189405413908 2202 38-39 0.20510909998382232 2202 40-41 0.23122146955123668 2202 42-43 0.16214084658499672 2202 44-45 0.21527218151334182 2202 46-47 0.27336995625998384 2202 48-49 0.2894056153403355 2202 50-51 0.27848358578366117 2202 52-53 0.28977617838511804 2202 54-55 0.21581846580913577 2202 56-57 0.2159454011510249 2202 58-59 0.28086515469523476 2202 60-61 0.2752437745025986 2202 62-63 0.2407418032147035 2202 64-65 0.24788666354765354 2202 66-67 0.08995495213939364 2202 68-69 0.11374863530033963 2202 70-71 0.19860604550391514 2202 72-73 0.17061482288566765 2202 74-75 0.08820103903692456 2202 76-77 -0.11980351995886451 2202 78-79 0.14353201480613365 2202 80-81 0.21502584464504082 2202 82-83 0.2373225651477 2202 84-85 0.20366092326865015 2202 86-87 0.3739504435300631 2202 88-89 0.41191852922277405 2202 90-91 0.2890459657878566 2202 92-93 0.41712146651347126 2202 94-95 0.3330372216165287 2202 96-97 0.3180332465923996 2202 98-99 0.2566346494763465 2202 100-101 0.23100049486827245 1102 1 0.11066106177428026 1102 2 0.0506394242582644 1102 3 0.28165162569671054 1102 4 0.1043952992110988 1102 5 0.04374907541411943 1102 6 0.018014791384381112 1102 7 0.05316484575306646 1102 8 0.09986928149783125 1102 9 0.03189995796995504 1102 10-11 0.09016488848065052 1102 12-13 0.08593782424532748 1102 14-15 0.09451829895598962 1102 16-17 0.124699920287064 1102 18-19 0.13353030548114475 1102 20-21 0.1444076654029658 1102 22-23 0.19335306584579826 1102 24-25 0.10254991920444922 1102 26-27 0.18237402766476407 1102 28-29 0.23037868423877939 1102 30-31 0.2547338974375464 1102 32-33 0.21352499361704247 1102 34-35 0.16512003278129583 1102 36-37 0.20344119289274687 1102 38-39 0.23192040555573357 1102 40-41 0.19075607115981086 1102 42-43 0.18169528306098215 1102 44-45 0.21383051504835038 1102 46-47 0.17893555081525392 1102 48-49 0.2192608218165617 1102 50-51 0.14176185475535164 1102 52-53 0.09331075780929865 1102 54-55 0.1240052140606025 1102 56-57 0.15228430007891092 1102 58-59 0.17034776750625724 1102 60-61 0.1225573953188217 1102 62-63 0.1870428911912967 1102 64-65 0.22027529115364786 1102 66-67 0.11393013384977024 1102 68-69 0.05303032806391883 1102 70-71 0.04978323026364251 1102 72-73 0.08959533893506944 1102 74-75 0.036091807101080065 1102 76-77 -0.036353029482018684 1102 78-79 0.015556381340765313 1102 80-81 0.11041878409104555 1102 82-83 0.0595867752621686 1102 84-85 0.04593135845929197 1102 86-87 0.11835501768376133 1102 88-89 0.19266494341755447 1102 90-91 0.13482605656910351 1102 92-93 0.23294689077324904 1102 94-95 0.176941067046414 1102 96-97 0.10805722590847466 1102 98-99 -0.024768725375416523 1102 100-101 -0.060042571768274655 2305 1 0.0964023188351888 2305 2 0.032056678618772594 2305 3 0.25857186842793567 2305 4 0.11964693642632795 2305 5 0.06250955950347503 2305 6 0.0375080460957804 2305 7 0.06298516209700011 2305 8 0.05620298734451268 2305 9 0.020161439454263075 2305 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-0.2568076552349652 1213 84-85 -0.25804511318751366 1213 86-87 -0.21874763916714102 1213 88-89 -0.1995551594811502 1213 90-91 -0.12120032561622196 1213 92-93 -0.08160896829337361 1213 94-95 0.028770885959595205 1213 96-97 0.03976346783640139 1213 98-99 0.052214513110264704 1213 100-101 0.3006602016158446 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 9.0 8 42.0 9 197.0 10 297.0 11 219.0 12 293.0 13 638.0 14 1512.0 15 3038.0 16 5559.0 17 8443.0 18 10814.0 19 13266.0 20 16195.0 21 19819.0 22 24024.0 23 28965.0 24 34434.0 25 42109.0 26 50935.0 27 62472.0 28 79009.0 29 100020.0 30 116307.0 31 142591.0 32 186629.0 33 260295.0 34 391266.0 35 669497.0 36 1295939.0 37 1999704.0 38 487899.0 39 192.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.06164823018552 28.968518556213603 16.45901732670113 16.510815886899746 2 33.36947190542687 23.479619100992164 28.402175055199162 14.748733938381807 3 39.21177353433039 20.752529563201165 21.755466139820964 18.280230762647488 4 33.30438067588591 20.589388190025943 27.243756587473218 18.862474546614934 5 35.11063293498295 18.537071830616387 26.93542375312013 19.41687148128053 6 32.1038233309564 19.542370685923537 28.715840458062186 19.637965525057876 7 35.88599861085135 20.083342310150236 22.257240986890324 21.77341809210809 8 31.065233151616123 26.014121469219653 22.18099311571767 20.739652263446555 9 29.411158260510973 22.582587266225516 26.89859677482244 21.107657698441074 10-11 22.578260962589994 30.138104689730564 31.43605532051954 15.847579027159897 12-13 22.467184706146213 29.318952801902974 31.56987605795995 16.643986433990857 14-15 22.39258715387762 29.01011758859127 31.831470561217373 16.765824696313732 16-17 22.1513365764425 28.89841404427961 31.83085925650808 17.11939012276981 18-19 21.038142439945094 29.225230759266886 31.916896263903876 17.819730536884144 20-21 21.61563539011484 29.054098484162584 31.22339585383407 18.10687027188851 22-23 20.96718978929483 28.559726452707817 31.540357345602605 18.93272641239475 24-25 20.529603008808735 27.797016436496673 32.18144250728774 19.491938047406844 26-27 20.51156147030739 28.220967908885157 31.64609308803262 19.621377532774826 28-29 20.674641725401564 28.80949076598234 30.980740571851527 19.535126936764573 30-31 19.748520807821 30.430921906979908 30.528995008449222 19.29156227674987 32-33 19.428007140039007 31.126462753038847 31.05472531931584 18.39080478760631 34-35 19.991729212500754 30.301746613206692 32.210107741628924 17.49641643266363 36-37 19.680053002322158 29.94055412870411 33.16777267158634 17.21162019738739 38-39 20.152118236237367 29.428900284002175 31.793183898243964 18.625797581516487 40-41 20.360394877516498 29.115566819014145 31.39435195818796 19.1296863452814 42-43 20.38513064353962 28.817521342703305 31.40497328790599 19.392374725851084 44-45 20.01828963797834 28.334920115008284 31.820161806295882 19.826628440717492 46-47 20.700683773251967 28.656162922587487 31.01588696799393 19.627266336166624 48-49 20.449175330549323 29.772479625935063 30.454726824372045 19.323618219143565 50-51 21.438136127888 29.58909039148918 28.922072844122145 20.05070063650067 52-53 21.100658606122344 29.524526909020526 28.797650387982227 20.577164096874903 54-55 21.71170453698666 29.348012326600387 29.170145384951812 19.770137751461146 56-57 21.199740783077008 30.055613992822956 29.05495702795438 19.689688196145653 58-59 20.801878966703814 31.04979754194907 28.764022682175895 19.384300809171222 60-61 20.967837072803853 30.98666935872415 28.530789250892045 19.51470431757995 62-63 20.390248681778726 30.713671797681414 29.2879077217019 19.60817179883796 64-65 20.80156714306864 29.654436724723197 29.906624750166554 19.6373713820416 66-67 21.036090030364704 29.63478985690523 29.326581802380474 20.00253831034959 68-69 21.220223413740136 30.336475734597236 29.463098062254744 18.980202789407883 70-71 20.51770986129446 30.929004219403705 29.568406826404225 18.984879092897614 72-73 20.296129474926925 30.335679837230916 29.65776451628682 19.710426171555337 74-75 20.5958945243027 29.404956555279355 29.331267936708766 20.667880983709182 76-77 20.594134508205457 28.70804829821745 28.936855856443934 21.760961337133157 78-79 19.910636828268373 29.274155569252326 29.325967371205824 21.489240231273474 80-81 19.45697013836385 29.877646201999585 28.98010854450959 21.68527511512697 82-83 18.624486508728577 30.09769500070342 29.038490563045084 22.23932792752292 84-85 18.3649265509839 30.485081978792454 30.03165311196395 21.118338358259695 86-87 18.81225461683874 30.175378732798393 30.65068674236916 20.361679907993707 88-89 19.602844836916784 29.975238219564364 30.086158106901156 20.33575883661769 90-91 19.536259953546672 30.165982646517612 29.485328201598517 20.8124291983372 92-93 18.93521584170589 30.56050543221306 29.72145107339565 20.782827652685402 94-95 19.07242203666961 30.02443847529562 29.817674894242614 21.085464593792153 96-97 19.379241117502495 30.68309538757788 29.397446285582173 20.540217209337452 98-99 20.17791736920548 30.444765326970185 28.767200149933707 20.610117153890624 100-101 19.870728921150427 30.96498030361558 27.967543828844928 21.196746946389062 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 12659.0 1 7134.0 2 1701.0 3 1856.0 4 1561.0 5 1049.0 6 903.5 7 944.5 8 984.5 9 941.5 10 899.0 11 843.5 12 812.5 13 828.0 14 791.5 15 794.5 16 854.0 17 946.5 18 1057.0 19 1227.0 20 1503.5 21 1964.5 22 2698.5 23 3820.5 24 5537.5 25 7815.5 26 10477.0 27 14032.0 28 18868.5 29 24737.5 30 32246.0 31 41620.0 32 59162.0 33 94372.5 34 142963.5 35 188066.0 36 245228.5 37 331171.5 38 412019.0 39 455410.0 40 458276.0 41 446400.5 42 447824.0 43 442426.5 44 393381.0 45 325696.0 46 280385.0 47 244583.0 48 207224.0 49 166235.5 50 121483.0 51 91639.0 52 74729.5 53 57402.5 54 41411.0 55 30187.5 56 22803.0 57 17448.5 58 13113.0 59 9724.5 60 7299.5 61 5786.5 62 4602.0 63 3583.5 64 2730.5 65 2029.5 66 1531.5 67 1276.5 68 1121.5 69 953.0 70 774.5 71 599.0 72 451.5 73 342.5 74 251.5 75 183.0 76 154.0 77 118.0 78 83.0 79 62.5 80 42.5 81 31.5 82 25.5 83 17.0 84 11.0 85 8.0 86 5.5 87 3.5 88 2.5 89 2.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1650357497602694 2 0.0 3 3.304349779963348E-5 4 0.022634795992748934 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.5695661454825902E-4 12-13 8.26087444990837E-6 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 4.130437224954185E-5 28-29 1.3217399119853392E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0010408701806884546 38-39 5.534785881438608E-4 40-41 2.808697312968846E-4 42-43 5.36956839244044E-4 44-45 3.386958524462432E-4 46-47 0.003023480048666464 48-49 0.003353915026662798 50-51 0.003940437112606293 52-53 0.003709132628008858 54-55 0.0057991338638356755 56-57 0.009153048890498474 58-59 0.016587835895416007 60-61 0.004229567718353085 62-63 0.0025443493305717783 64-65 0.009846962344290779 66-67 0.022444795880401044 68-69 0.025162623574420895 70-71 0.025377406310118516 72-73 0.0224034915081515 74-75 0.024617405860726943 76-77 0.02433653612943006 78-79 0.015166965490031768 80-81 0.0029739148019670134 82-83 0.0010986963018378131 84-85 0.00496478554439493 86-87 0.01434087804504093 88-89 0.015910444190523523 90-91 0.02301479621744472 92-93 0.023312187697641422 94-95 0.025013927834322543 96-97 0.02641827649080697 98-99 0.03228349735024191 100-101 0.022973491845195176 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6052628.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.813750900584814 #Duplication Level Percentage of deduplicated Percentage of total 1 80.56792663343806 28.85439655026544 2 8.600858009073397 6.160579725365089 3 3.6497818711368692 3.9213713632309855 4 1.6969176726242123 2.4309194730465467 5 1.0295862992181355 1.8436673625426645 6 0.7468651732886001 1.604882596348802 7 0.4750447994087123 1.1909195278849332 8 0.42742906216799204 1.224627036812404 9 0.2580102224624092 0.8316282453365875 >10 2.088645208981536 14.207044041085098 >50 0.21759790907674198 5.453266290404199 >100 0.19884907871055366 15.064004935953607 >500 0.027464358683909326 6.854620558341957 >1k 0.014746524940945058 8.909508315548923 >5k 1.385904711154945E-4 0.36681365192793924 >10k+ 1.385863168218297E-4 1.0817503259048504 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT 29510 0.487556810033592 TruSeq Adapter, Index 7 (97% over 35bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 19328 0.31933236273565796 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16547 0.2733853790452676 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8243 0.1361887761811894 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 7723 0.1275974667532847 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 6205 0.10251745192336288 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 8.756526916902873E-4 0.0 0.0 2 0.001007826682888821 0.0 0.0 3 0.001073913678488088 0.0 0.0 4 0.0012060876696866221 0.0 0.0 5 0.0012886964141857057 0.0 0.0 6 0.0014373921542840565 0.0 0.0 7 0.0016026096432822238 0.0 0.0 8 0.0018669576256792916 0.0 0.0 9 0.0022965230970745268 0.0 0.0 10-11 0.0031473931654150892 0.0 0.0 12-13 0.004882176799895847 0.0 8.26087444990837E-6 14-15 0.008252613575458461 0.0 1.652174889981674E-5 16-17 0.016356531410818574 0.0 1.652174889981674E-5 18-19 0.026319145997408068 0.0 1.652174889981674E-5 20-21 0.03367132425782652 0.0 1.652174889981674E-5 22-23 0.04152741585968937 0.0 1.652174889981674E-5 24-25 0.05149829132072878 0.0 1.652174889981674E-5 26-27 0.06247699346465701 0.0 1.652174889981674E-5 28-29 0.07379439146103148 0.0 1.652174889981674E-5 30-31 0.08668961647733844 0.0 1.652174889981674E-5 32-33 0.10022918970073827 0.0 1.652174889981674E-5 34-35 0.11531354644627094 0.0 1.652174889981674E-5 36-37 0.1318600779694374 0.0 1.652174889981674E-5 38-39 0.14809269626350735 0.0 1.652174889981674E-5 40-41 0.16696053350709805 0.0 1.652174889981674E-5 42-43 0.18550619664714235 0.0 1.652174889981674E-5 44-45 0.20527446920577308 0.0 1.652174889981674E-5 46-47 0.22585230746049484 0.0 1.652174889981674E-5 48-49 0.24805753798184854 0.0 1.652174889981674E-5 50-51 0.27134494305614026 0.0 1.652174889981674E-5 52-53 0.29640017526271234 0.0 1.652174889981674E-5 54-55 0.3222319296675758 0.0 1.652174889981674E-5 56-57 0.35081455526425875 0.0 1.652174889981674E-5 58-59 0.38141283422671934 0.0 1.652174889981674E-5 60-61 0.41472068000874995 0.0 1.652174889981674E-5 62-63 0.4502259183944561 0.0 1.652174889981674E-5 64-65 0.48823420173848453 0.0 1.652174889981674E-5 66-67 0.5311577053802083 0.0 1.652174889981674E-5 68-69 0.5809873000620557 0.0 1.652174889981674E-5 70-71 0.6350216798389063 0.0 1.652174889981674E-5 72-73 0.6966560641096726 0.0 2.478262334972511E-5 74-75 0.7727882830400282 0.0 3.304349779963348E-5 76-77 0.8640379022137161 0.0 3.304349779963348E-5 78-79 0.9734531843027524 0.0 3.304349779963348E-5 80-81 1.108196307455208 0.0 3.304349779963348E-5 82-83 1.2761068415240455 0.0 3.304349779963348E-5 84-85 1.483430007593396 0.0 3.304349779963348E-5 86-87 1.7333710249498235 0.0 3.304349779963348E-5 88-89 2.0240133707209496 0.0 3.304349779963348E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGGAG 420 0.0 61.17794 1 GTTACGG 255 0.0 54.113815 1 CGACGGG 305 0.0 48.362885 1 CGAGTGG 330 0.0 47.58284 1 CGGAGCG 65 2.560335E-6 43.922623 1 CGGACGG 120 1.8189894E-12 43.617603 1 CGAGCGG 135 0.0 42.295856 1 CGGATGG 170 0.0 41.984856 1 CGAGGGG 970 0.0 41.20576 1 GCGCATA 140 0.0 40.717556 4 CCGGTTG 190 0.0 39.994286 6 TCAGCCG 430 0.0 38.665894 2 CCAGCCG 395 0.0 38.4841 2 GGGTACG 50 0.0016157234 38.00305 2 TGTACCG 150 0.0 38.00305 2 GTCGACC 105 4.5922206E-8 36.253593 1 ATTACGG 200 0.0 35.68713 1 GGACGGG 735 0.0 35.606205 1 CGCGGGG 390 0.0 35.38211 1 CGGGGTG 1325 0.0 35.193348 1 >>END_MODULE