FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n01_50110001-2.341000000358f7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n01_50110001-2.341000000358f7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6052628
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT295100.487556810033592TruSeq Adapter, Index 7 (97% over 35bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC193280.31933236273565796No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165470.2733853790452676No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA82430.1361887761811894No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG77230.1275974667532847No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA62050.10251745192336288No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAG4200.061.177941
GTTACGG2550.054.1138151
CGACGGG3050.048.3628851
CGAGTGG3300.047.582841
CGGAGCG652.560335E-643.9226231
CGGACGG1201.8189894E-1243.6176031
CGAGCGG1350.042.2958561
CGGATGG1700.041.9848561
CGAGGGG9700.041.205761
GCGCATA1400.040.7175564
CCGGTTG1900.039.9942866
TCAGCCG4300.038.6658942
CCAGCCG3950.038.48412
GGGTACG500.001615723438.003052
TGTACCG1500.038.003052
GTCGACC1054.5922206E-836.2535931
ATTACGG2000.035.687131
GGACGGG7350.035.6062051
CGCGGGG3900.035.382111
CGGGGTG13250.035.1933481
Produced by FastQC (version 0.11.2)