FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n01_44065-1.341000000357de.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n01_44065-1.341000000357de.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3920376
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT194310.4956412344122094TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTCG451.5016989E-552.8661651
CGAGGCG1800.050.2228551
AGCGCCC1650.049.021351
CGACGGG1600.047.5795481
CGCTCGG502.80042E-547.5795481
AGTCGCC804.1673047E-947.5795481
AACCGAG1800.044.936241
CGAGGGG4250.044.780751
CGGACGG554.9167218E-543.254131
CGTGCGG459.548958E-442.292931
CGATCGC459.548958E-442.292931
GCGCATA459.623046E-442.2265664
GATACGT459.63695E-442.214189
CGACGCC500.001603059138.0636371
CGGAGCG500.001603059138.0636371
GCGCCCC1003.031346E-838.003912
GCGCGTT500.001617804637.9927645
TTGTGTT241800.036.6390081
CGAGCCC1700.036.384361
GGTACGG801.06597545E-535.684661
Produced by FastQC (version 0.11.2)