##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n01_44065-1.341000000357de.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3920376 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.670974926894765 33.0 33.0 33.0 33.0 33.0 2 32.72698843172186 33.0 33.0 33.0 33.0 33.0 3 32.55677950278238 33.0 33.0 33.0 33.0 33.0 4 36.61337126847017 37.0 37.0 37.0 37.0 37.0 5 36.67631778176379 37.0 37.0 37.0 37.0 37.0 6 36.55456593959355 37.0 37.0 37.0 37.0 37.0 7 36.50752427828351 37.0 37.0 37.0 37.0 37.0 8 36.45451711774585 37.0 37.0 37.0 37.0 37.0 9 36.46273520703116 37.0 37.0 37.0 37.0 37.0 10-11 36.42395168218559 37.0 37.0 37.0 37.0 37.0 12-13 36.41511337177863 37.0 37.0 37.0 37.0 37.0 14-15 38.301566992553774 40.0 37.0 40.0 37.0 40.0 16-17 38.2849346593286 40.0 37.0 40.0 37.0 40.0 18-19 38.283422942085146 40.0 37.0 40.0 37.0 40.0 20-21 38.215153214895714 40.0 37.0 40.0 37.0 40.0 22-23 38.12740512644706 40.0 37.0 40.0 37.0 40.0 24-25 38.081022458049944 40.0 37.0 40.0 37.0 40.0 26-27 38.018678437986566 40.0 37.0 40.0 37.0 40.0 28-29 37.89523798227517 40.0 37.0 40.0 33.0 40.0 30-31 37.860247205880256 40.0 37.0 40.0 33.0 40.0 32-33 37.76179695519001 40.0 37.0 40.0 33.0 40.0 34-35 37.67650284054387 40.0 37.0 40.0 33.0 40.0 36-37 37.5981886686379 40.0 37.0 40.0 33.0 40.0 38-39 37.51593686421915 40.0 37.0 40.0 33.0 40.0 40-41 37.448329573489886 40.0 37.0 40.0 33.0 40.0 42-43 37.31535023681402 40.0 37.0 40.0 33.0 40.0 44-45 37.19373779453808 40.0 37.0 40.0 33.0 40.0 46-47 37.11171823825062 40.0 37.0 40.0 33.0 40.0 48-49 36.94270944929772 37.0 37.0 40.0 33.0 40.0 50-51 36.76851761157603 37.0 37.0 40.0 33.0 40.0 52-53 36.627301437413145 37.0 37.0 40.0 33.0 40.0 54-55 36.494105794954365 37.0 37.0 40.0 33.0 40.0 56-57 36.31532447397903 37.0 37.0 40.0 33.0 40.0 58-59 36.087491735486594 37.0 37.0 40.0 33.0 40.0 60-61 35.79171054000943 37.0 37.0 40.0 30.0 40.0 62-63 35.52504389885052 37.0 33.0 40.0 27.0 40.0 64-65 35.238630937440696 37.0 33.0 37.0 27.0 40.0 66-67 34.93945963346373 37.0 33.0 37.0 27.0 40.0 68-69 34.66546410344314 37.0 33.0 37.0 27.0 40.0 70-71 34.351046302701576 37.0 33.0 37.0 27.0 40.0 72-73 34.035244068426096 37.0 33.0 37.0 27.0 37.0 74-75 33.688150447814195 37.0 33.0 37.0 27.0 37.0 76-77 31.058690671507016 33.0 30.0 35.0 24.5 37.0 78-79 33.41265391380827 35.0 33.0 37.0 27.0 37.0 80-81 33.78877842329409 37.0 33.0 37.0 27.0 37.0 82-83 33.73498077735401 37.0 33.0 37.0 27.0 37.0 84-85 33.58706779655829 37.0 33.0 37.0 27.0 37.0 86-87 33.44609585917269 37.0 33.0 37.0 27.0 37.0 88-89 33.33419128675413 37.0 33.0 37.0 27.0 37.0 90-91 33.207904547931115 37.0 33.0 37.0 27.0 37.0 92-93 33.050215208949346 35.0 33.0 37.0 27.0 37.0 94-95 32.940346665727986 33.0 33.0 37.0 27.0 37.0 96-97 32.81019715966019 33.0 33.0 37.0 27.0 37.0 98-99 32.64367741767626 33.0 33.0 37.0 27.0 37.0 100-101 30.959406954843107 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 0.17808035652221577 2306 2 0.11065645251508016 2306 3 0.30184388688727637 2306 4 0.23779357587518035 2306 5 0.11046970888566676 2306 6 0.13817760343561503 2306 7 0.17762122989970663 2306 8 0.2042225061577696 2306 9 0.12957559106516214 2306 10-11 0.17274445938552674 2306 12-13 0.2001003142600979 2306 14-15 0.34037111400903797 2306 16-17 0.33472444380517885 2306 18-19 0.33357738183073593 2306 20-21 0.36861877110128205 2306 22-23 0.41520785020301076 2306 24-25 0.38598627618972614 2306 26-27 0.4115970458378939 2306 28-29 0.4895978660226987 2306 30-31 0.4023232338914582 2306 32-33 0.48783697011898397 2306 34-35 0.46337689116504777 2306 36-37 0.5396472486312192 2306 38-39 0.5860171570451982 2306 40-41 0.48671322246158155 2306 42-43 0.5542530965911254 2306 44-45 0.39300113015233507 2306 46-47 0.5228484792242156 2306 48-49 0.49727607397847606 2306 50-51 0.46194505544185915 2306 52-53 0.48748788139810273 2306 54-55 0.5889288444674108 2306 56-57 0.5375974658298261 2306 58-59 0.5482718633395223 2306 60-61 0.5575567678362816 2306 62-63 0.6606564725476787 2306 64-65 0.5094002344917783 2306 66-67 0.4174157538149217 2306 68-69 0.45733053458836537 2306 70-71 0.46942471797957097 2306 72-73 0.48388962247778267 2306 74-75 0.4259665764663936 2306 76-77 0.29036744630352374 2306 78-79 0.48564126183767087 2306 80-81 0.48755702344882224 2306 82-83 0.4742501528054248 2306 84-85 0.6021430599427333 2306 86-87 0.5807843204924836 2306 88-89 0.6073518754413385 2306 90-91 0.5594660882319786 2306 92-93 0.5072804771254482 2306 94-95 0.6192587385034543 2306 96-97 0.6037905862432353 2306 98-99 0.5291561507443774 2306 100-101 0.5263235867453027 2307 1 0.16384234078169158 2307 2 0.12556333194616798 2307 3 0.32921959965531045 2307 4 0.2235463456429727 2307 5 0.17292715961524152 2307 6 0.22255741394230455 2307 7 0.16790969769234465 2307 8 0.16633360040575695 2307 9 0.24370465899222893 2307 10-11 0.18516830432189124 2307 12-13 0.20964788271835744 2307 14-15 0.42284000154887735 2307 16-17 0.32960145744262803 2307 18-19 0.38973970865249896 2307 20-21 0.42421386465547783 2307 22-23 0.42704711694629083 2307 24-25 0.35988541544780617 2307 26-27 0.41920415075301065 2307 28-29 0.47796673838572445 2307 30-31 0.46675041772599 2307 32-33 0.45342707700709184 2307 34-35 0.4587509453245815 2307 36-37 0.5308810148649812 2307 38-39 0.4667016853716319 2307 40-41 0.47009380264933753 2307 42-43 0.4788324821471406 2307 44-45 0.43841475242321337 2307 46-47 0.45369263506837143 2307 48-49 0.519117942460575 2307 50-51 0.3236368903181699 2307 52-53 0.3705727848085516 2307 54-55 0.38597966509919956 2307 56-57 0.3823424954246022 2307 58-59 0.34742087793093646 2307 60-61 0.3715256662166979 2307 62-63 0.4250774230495935 2307 64-65 0.34978301531695877 2307 66-67 0.3036156974439166 2307 68-69 0.4426385696251103 2307 70-71 0.36351849982376905 2307 72-73 0.27805955938561766 2307 74-75 0.2742204302920328 2307 76-77 -0.10510308900424192 2307 78-79 0.147286482226626 2307 80-81 0.3276219043108668 2307 82-83 0.424067489058487 2307 84-85 0.41213726023325137 2307 86-87 0.362208989312812 2307 88-89 0.4176815916349952 2307 90-91 0.31675258303933873 2307 92-93 0.3152705254744248 2307 94-95 0.29933131617620035 2307 96-97 0.35085848078608706 2307 98-99 0.14350365632727602 2307 100-101 0.18751296081030588 2201 1 0.14184332507769426 2201 2 0.15233516643801437 2201 3 0.32805491717212476 2201 4 0.20936546701643266 2201 5 0.1103004111679482 2201 6 0.13409950479852029 2201 7 0.2144021263410565 2201 8 0.18467912897678218 2201 9 0.1750112712650136 2201 10-11 0.16470318378630822 2201 12-13 0.2098071698765409 2201 14-15 0.37620069112550425 2201 16-17 0.35411169900188355 2201 18-19 0.32100035999614107 2201 20-21 0.33299530010070555 2201 22-23 0.3990624528474669 2201 24-25 0.3445708677968895 2201 26-27 0.36853268394860095 2201 28-29 0.4902937748006124 2201 30-31 0.39766331159691504 2201 32-33 0.3882366340332126 2201 34-35 0.46592995094157885 2201 36-37 0.44800929059801575 2201 38-39 0.4046540751365839 2201 40-41 0.46642735087374376 2201 42-43 0.3985337564444009 2201 44-45 0.4471583616170278 2201 46-47 0.5115424973889517 2201 48-49 0.45326452325216593 2201 50-51 0.4490386925560159 2201 52-53 0.4924454079574119 2201 54-55 0.5793839667167759 2201 56-57 0.5863633649403042 2201 58-59 0.6250926111460515 2201 60-61 0.47827780462010594 2201 62-63 0.48064494900938826 2201 64-65 0.5587233311864992 2201 66-67 0.5448182468519107 2201 68-69 0.5368196821236424 2201 70-71 0.5759653193681444 2201 72-73 0.5468272777305359 2201 74-75 0.4869349824170115 2201 76-77 0.3404816621356268 2201 78-79 0.5831476497155776 2201 80-81 0.5773496468157759 2201 82-83 0.6762364991902317 2201 84-85 0.6725281416662625 2201 86-87 0.6645190344195981 2201 88-89 0.6136375891295174 2201 90-91 0.5769440123428922 2201 92-93 0.43586193688167896 2201 94-95 0.52827648234733 2201 96-97 0.5958831593646323 2201 98-99 0.4671001193694053 2201 100-101 0.3480981593574519 1101 1 0.10310978414182159 1101 2 0.08412311402385342 1101 3 0.31103133291152574 1101 4 0.17241989911327238 1101 5 0.09332366801204728 1101 6 0.09335226759903037 1101 7 0.14566577399642 1101 8 0.09840073579820086 1101 9 0.08634303926978504 1101 10-11 0.11690216630703532 1101 12-13 0.14435633932343706 1101 14-15 0.18515123455300397 1101 16-17 0.21836273754080082 1101 18-19 0.24131110595817518 1101 20-21 0.21078615108105936 1101 22-23 0.2567436730233723 1101 24-25 0.2259302192747299 1101 26-27 0.26036234179459683 1101 28-29 0.33368548924064356 1101 30-31 0.2485444662895233 1101 32-33 0.1904364977699018 1101 34-35 0.3704795605252045 1101 36-37 0.37530779632908207 1101 38-39 0.4044067691833959 1101 40-41 0.3697119207635424 1101 42-43 0.36692618952311307 1101 44-45 0.24700321106057999 1101 46-47 0.35625203023233354 1101 48-49 0.31509848539041485 1101 50-51 0.23686870439635754 1101 52-53 0.3017333110112119 1101 54-55 0.37286299693437996 1101 56-57 0.4260618891816179 1101 58-59 0.4077585090028535 1101 60-61 0.43360406705149757 1101 62-63 0.4732394363297203 1101 64-65 0.3490854958638039 1101 66-67 0.22767625921136414 1101 68-69 0.37870239670985484 1101 70-71 0.3135177670326357 1101 72-73 0.39829983671285873 1101 74-75 0.28044949689200394 1101 76-77 0.007481133123313555 1101 78-79 0.39183505357703297 1101 80-81 0.33994818395959214 1101 82-83 0.3803394972188485 1101 84-85 0.45816274768591825 1101 86-87 0.49911098289551603 1101 88-89 0.5901349470367379 1101 90-91 0.5956262146740343 1101 92-93 0.5958551755520034 1101 94-95 0.431292587676289 1101 96-97 0.4598025873866831 1101 98-99 0.31505983111848224 1101 100-101 0.3458486553595108 2304 1 0.08731320953557997 2304 2 0.035580673095587656 2304 3 0.2679421978118768 2304 4 0.0921206958210945 2304 5 0.0979742823038734 2304 6 0.09195688239837807 2304 7 0.08555196108932961 2304 8 0.09204279686966998 2304 9 0.1038704366108334 2304 10-11 0.08303883002260903 2304 12-13 0.07894580178042077 2304 14-15 0.17229078661286934 2304 16-17 0.25897797714933546 2304 18-19 0.24991302590128583 2304 20-21 0.19223287394806476 2304 22-23 0.3230767948155844 2304 24-25 0.3030073878150574 2304 26-27 0.2536048915216824 2304 28-29 0.2813395448245686 2304 30-31 0.19662384618431616 2304 32-33 0.29057884033809955 2304 34-35 0.28499710482545737 2304 36-37 0.2889941281667774 2304 38-39 0.24085795363166795 2304 40-41 0.3426356475968433 2304 42-43 0.28754782340964624 2304 44-45 0.2887947969070339 2304 46-47 0.4180589291516412 2304 48-49 0.22740611655063958 2304 50-51 0.2900160300463668 2304 52-53 0.22175980533520345 2304 54-55 0.3267327799089159 2304 56-57 0.37226530846062644 2304 58-59 0.20895839701562835 2304 60-61 0.39229899665983936 2304 62-63 0.3948319609645097 2304 64-65 0.263120149880514 2304 66-67 0.2225062408627565 2304 68-69 0.22789977068820377 2304 70-71 0.2144993585876449 2304 72-73 0.16961328867519399 2304 74-75 0.12205584958971372 2304 76-77 -0.08494942191661536 2304 78-79 0.12722847419692584 2304 80-81 0.24949649198734392 2304 82-83 0.333045774923562 2304 84-85 0.24505080894869025 2304 86-87 0.30210965468517514 2304 88-89 0.3234689646393534 2304 90-91 0.30710232763437517 2304 92-93 0.44244382387414305 2304 94-95 0.42454548879603493 2304 96-97 0.19510315121000588 2304 98-99 0.1974072998535661 2304 100-101 0.04328242719298103 2202 1 0.10114680282842414 2202 2 0.07840245437292026 2202 3 0.2723302226628448 2202 4 0.11761724409531382 2202 5 0.10695293462039501 2202 6 0.1083464229271911 2202 7 0.10916879384834743 2202 8 0.10469450113779288 2202 9 0.08561581971782317 2202 10-11 0.11673237274163739 2202 12-13 0.09825633820825175 2202 14-15 0.0938432564438827 2202 16-17 0.05657189923265804 2202 18-19 0.08755473146752024 2202 20-21 0.1184830149813294 2202 22-23 0.1730354452799716 2202 24-25 0.17681430711895985 2202 26-27 0.22351860545406765 2202 28-29 0.17242486802426527 2202 30-31 0.1055449124483161 2202 32-33 0.14482453786331462 2202 34-35 0.09640998105062693 2202 36-37 0.1188336515289592 2202 38-39 0.1468204555677275 2202 40-41 0.09264290651287865 2202 42-43 0.06703226648093619 2202 44-45 0.14495769749709098 2202 46-47 0.2648936171456384 2202 48-49 0.2860510407081094 2202 50-51 0.2746700345214208 2202 52-53 0.18545058602656184 2202 54-55 0.23258156556708087 2202 56-57 0.3315870304938855 2202 58-59 0.23073660093738368 2202 60-61 0.2848030234373553 2202 62-63 0.21601161772090904 2202 64-65 0.10384602530780285 2202 66-67 0.19531725306299563 2202 68-69 0.17561600247368148 2202 70-71 0.23232592185284062 2202 72-73 0.11801606429057188 2202 74-75 0.09802463180242427 2202 76-77 -0.1601636003791853 2202 78-79 0.17580946732177694 2202 80-81 0.20756915474780868 2202 82-83 0.2910033464582682 2202 84-85 0.32329622177881845 2202 86-87 0.42143487070364216 2202 88-89 0.36514258355474993 2202 90-91 0.22912923536444652 2202 92-93 0.22485690593708796 2202 94-95 0.18640305956238024 2202 96-97 0.36242941542403173 2202 98-99 0.22059256073577416 2202 100-101 0.14746558480932137 1102 1 0.12265650711675136 1102 2 0.06814734959982616 1102 3 0.2670863290262204 1102 4 0.17785756367285188 1102 5 0.06829217064877469 1102 6 0.02737780246742716 1102 7 0.08698595569617851 1102 8 0.03489526158580247 1102 9 0.09019472881098523 1102 10-11 0.048610224518689904 1102 12-13 0.04936092037706885 1102 14-15 0.05528175895581455 1102 16-17 0.11802483768355643 1102 18-19 0.104568960180913 1102 20-21 0.08397537751587691 1102 22-23 0.0977300203938185 1102 24-25 0.056951580480543385 1102 26-27 0.06981687368467959 1102 28-29 0.16835354517225198 1102 30-31 0.06640403708713194 1102 32-33 0.1013808989511702 1102 34-35 0.06790742255208926 1102 36-37 0.005385410191102835 1102 38-39 0.08937605139558258 1102 40-41 0.14414645612399823 1102 42-43 0.09949865441497963 1102 44-45 0.25849352462594766 1102 46-47 0.25126073549161987 1102 48-49 0.17538489973728844 1102 50-51 0.11238404149568737 1102 52-53 0.06152980828647259 1102 54-55 0.11202513773241662 1102 56-57 0.12567625080571787 1102 58-59 0.07923363342057854 1102 60-61 0.08406636368079745 1102 62-63 0.12804245940599657 1102 64-65 -0.019387299048332807 1102 66-67 -0.09498792734355277 1102 68-69 -0.05095881488452392 1102 70-71 -0.04431847517894738 1102 72-73 -0.0548836812177953 1102 74-75 -0.10833618440246084 1102 76-77 -0.12685950111580624 1102 78-79 0.11936576088668716 1102 80-81 0.16535614082643946 1102 82-83 0.01925343125000012 1102 84-85 -0.01931238343783548 1102 86-87 0.14207007530202986 1102 88-89 0.17119830472691433 1102 90-91 0.03846241916422599 1102 92-93 0.03242405439675622 1102 94-95 0.04039098770244465 1102 96-97 -0.015628966307211556 1102 98-99 -0.1025061834151515 1102 100-101 -0.0048065233267280405 2305 1 0.0788258148911325 2305 2 0.021959352378175367 2305 3 0.23698342965564478 2305 4 0.1515727536822311 2305 5 0.03280513511018057 2305 6 0.055163496805455736 2305 7 0.03833252679126531 2305 8 0.02002605191312057 2305 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0.028327902297093033 1213 90-91 0.1465886814529327 1213 92-93 0.08034798364677442 1213 94-95 0.18473565791060054 1213 96-97 0.1353647083669145 1213 98-99 0.16268954891205567 1213 100-101 0.47679618570375837 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 10.0 8 44.0 9 184.0 10 261.0 11 207.0 12 153.0 13 242.0 14 463.0 15 955.0 16 1736.0 17 2814.0 18 3997.0 19 5056.0 20 6583.0 21 8440.0 22 10785.0 23 13489.0 24 16769.0 25 20973.0 26 26755.0 27 33989.0 28 44273.0 29 58056.0 30 70147.0 31 86035.0 32 116941.0 33 166033.0 34 254617.0 35 439597.0 36 855292.0 37 1329548.0 38 345123.0 39 809.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 32.9283648293695 32.26030477397464 15.813292724057929 18.99803767259794 2 30.759013931316794 24.696789287558133 29.948938571198276 14.595258209926804 3 32.67076882735393 23.183278733936795 24.12645429401743 20.019498144691845 4 28.401701549207843 22.836228312531716 28.66349601363648 20.098574124623962 5 31.472567937361113 21.038492226255848 27.9009207280118 19.58801910837124 6 28.49749615853173 21.509518474758544 30.17218246413099 19.820802902578734 7 31.24093199223748 22.30301379255459 24.564582580854488 21.89147163435344 8 27.62689089005748 25.252679844994457 25.32190789863013 21.798521366317924 9 26.808321446718374 22.644409617852983 28.281930100582187 22.265338834846453 10-11 22.004687565961543 29.25453620913222 31.06325510222692 17.67752112267932 12-13 22.068360807320726 28.639262825622545 30.13446417753404 19.157912189522687 14-15 22.099525657743033 28.457334194475226 30.384445267494755 19.058694880286993 16-17 22.394854473142374 27.791734772378973 30.65435560262587 19.15905515185278 18-19 21.58768699736964 27.897706750576983 31.003352739635176 19.511253512418197 20-21 21.970010019447113 28.11320903913298 30.551878187194287 19.364902754225614 22-23 21.57710127804068 28.930974988113384 29.960098215069163 19.53182551877677 24-25 21.331091711611336 28.169211320546804 30.549926843751724 19.949770124090136 26-27 21.297258387621287 28.290593592373998 30.51949998157064 19.892648038434075 28-29 21.379200221611537 29.131777877796594 30.047404922005516 19.441616978586353 30-31 20.708638661189642 29.458220333967965 30.27326970678323 19.559871298059168 32-33 20.542723453056546 29.816094170559147 29.985810034547704 19.6553723418366 34-35 21.041145033027444 29.455835358649274 30.266408120037465 19.236611488285817 36-37 20.607795462221056 28.953083565371507 31.150894873189312 19.28822609921813 38-39 20.937451893650834 29.041306383192012 30.478078426916166 19.543163296240987 40-41 20.80214469826151 29.13649621910205 30.345809637623027 19.715549445013412 42-43 21.092074848342776 29.138031922627395 29.994179097091745 19.775714131938084 44-45 20.639621565849097 28.812342728291878 30.418506950245707 20.129528755613315 46-47 20.997028250749313 29.016606083795676 30.06522543205153 19.92114023340348 48-49 20.6818737210611 29.76219325235534 29.929466089083277 19.626466937500286 50-51 21.201713223891108 29.335878944286552 29.35957664529069 20.102831186531645 52-53 20.74691395185581 29.09615624005159 29.311539340609112 20.84539046748349 54-55 21.27621147644443 29.14516442550178 29.552994872114063 20.025629225939724 56-57 20.962944586202962 28.85315908196604 29.715784982554243 20.468111349276754 58-59 21.001383777252684 29.351066125877807 29.602972773978458 20.04457732289105 60-61 21.046316796670382 29.520968081162007 29.338847665883076 20.09386745628453 62-63 20.61898008849642 29.499615013909114 29.226445158198867 20.6549597393956 64-65 20.873422389275635 29.272171818639965 29.2846081367194 20.569797655365008 66-67 20.98858981875666 29.502495482821455 29.06151884356147 20.44739585486041 68-69 21.060042118885068 29.67002195754331 29.14131615647569 20.128619767095934 70-71 20.989066741950847 30.162552848077812 28.979212611001536 19.86916779896981 72-73 20.646418531397657 30.161984146655875 28.92705259882763 20.264544723118846 74-75 20.83218945310108 29.606371676538906 28.865851679641413 20.695587190718598 76-77 20.905993975127434 29.22535202283808 28.976833045918287 20.8918209561162 78-79 20.61094739731633 28.823179152100593 29.697641271285306 20.86823217929777 80-81 20.552186441075726 29.259585863357763 29.392064591766037 20.79616310380047 82-83 20.099784890722294 29.572526109389173 29.2300934639991 21.097595535889425 84-85 20.123800706168087 29.568897687256797 29.34576969400446 20.96153191257066 86-87 20.29434342336578 29.330459723930424 29.84022946015992 20.53496739254387 88-89 20.440626107774225 29.82969153187564 29.51973478856572 20.209947571784408 90-91 20.747710334513002 29.59412255619972 28.94007820977334 20.718088899513937 92-93 20.39506583580137 29.684805105208696 28.99979793072902 20.92033112826092 94-95 19.96539261357845 29.85998219878096 28.97527921723623 21.199345970404355 96-97 20.04025170583698 30.258382986714334 28.82709499081095 20.874270316637737 98-99 20.287036668473196 29.994582962076144 28.762072875617868 20.956307493832792 100-101 20.432724475949467 30.01540651604927 28.319286490907963 21.2325825170933 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 189.0 1 125.5 2 92.0 3 163.0 4 162.5 5 92.0 6 68.5 7 74.0 8 72.0 9 76.5 10 78.5 11 80.5 12 91.0 13 91.5 14 99.0 15 118.0 16 131.5 17 167.0 18 220.5 19 284.5 20 443.5 21 743.5 22 1171.5 23 1827.0 24 2841.0 25 4479.0 26 7405.5 27 11815.0 28 16819.5 29 22127.0 30 28138.0 31 32914.5 32 40094.0 33 55598.0 34 78238.5 35 111403.0 36 155408.0 37 208025.5 38 259757.0 39 278353.5 40 284627.5 41 273800.0 42 240356.0 43 225524.0 44 212931.5 45 203133.5 46 207179.5 47 193798.0 48 158819.0 49 129037.5 50 104026.0 51 84549.0 52 70009.5 53 55938.0 54 45287.0 55 34960.5 56 24179.0 57 15648.5 58 10211.5 59 7166.5 60 5329.0 61 4002.5 62 2915.5 63 1957.0 64 1336.0 65 954.5 66 712.5 67 561.0 68 421.0 69 298.5 70 202.0 71 147.5 72 105.5 73 62.5 74 40.5 75 37.0 76 34.0 77 22.0 78 15.0 79 14.0 80 12.5 81 8.0 82 5.5 83 5.0 84 2.5 85 3.0 86 2.5 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16977963338210417 2 0.0 3 7.652327225755897E-5 4 0.022676396345656644 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.1478490838633846E-4 12-13 2.5507757419186325E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.2753878709593162E-5 28-29 6.376939354796581E-5 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0011223413264441983 38-39 6.504478141892512E-4 40-41 2.8058533161104957E-4 42-43 5.611706632220991E-4 44-45 2.8058533161104957E-4 46-47 0.0032139774348174765 48-49 0.0034180394941709675 50-51 0.00419602609545615 52-53 0.003877179127716321 54-55 0.00617287729544309 56-57 0.00939960860897016 58-59 0.017090197470854836 60-61 0.004272549367713709 62-63 0.002665560650304971 64-65 0.010343395633480055 66-67 0.022803935132752574 68-69 0.025354710874671206 70-71 0.025622542327572658 72-73 0.022638134709527864 74-75 0.02469150918177236 76-77 0.02441092385016131 78-79 0.015177115664415862 80-81 0.0031629619199791043 82-83 0.001096833569025012 84-85 0.005216336392223603 86-87 0.014462898456678645 88-89 0.016210179839892907 90-91 0.023390613553393856 92-93 0.02367119888500491 94-95 0.025035863906931376 96-97 0.02650255995853459 98-99 0.03213977434817476 100-101 0.023734968278552874 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3920376.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.07337111889938 #Duplication Level Percentage of deduplicated Percentage of total 1 87.36313324222763 36.75661527010098 2 5.818146386882933 4.895780643188183 3 1.885459504911454 2.3798291243938765 4 0.9481745705391311 1.5957160236718357 5 0.6345977551582653 1.3349833431997071 6 0.3905827216578842 0.9859879080565164 7 0.3575261577171258 1.0529631502842747 8 0.27405427757903916 0.9224309861843824 9 0.2293740709709243 0.8685486371711216 >10 1.6649554325287645 14.52159974348565 >50 0.2245879047919265 6.5929845858396465 >100 0.17415708175670802 14.187848296002961 >500 0.025091084150318785 7.420220635455867 >1k 0.010098581566636698 5.983939394608717 >5k 0.0 0.0 >10k+ 6.122756135921354E-5 0.5005522583562957 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT 19431 0.4956412344122094 TruSeq Adapter, Index 2 (97% over 37bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 9.692947819290803E-4 0.0 0.0 2 0.0011223413264441983 0.0 0.0 3 0.0014029266580552477 0.0 0.0 4 0.0016069887174087383 0.0 0.0 5 0.001989605078696533 0.0 0.0 6 0.0029844076180448 0.0 0.0 7 0.003622101553524458 0.0 0.0 8 0.004744442879968656 0.0 0.0 9 0.0054076445728675005 0.0 0.0 10-11 0.007524788438659965 0.0 0.0 12-13 0.010815289145735001 0.0 0.0 14-15 0.015840317357314705 0.0 0.0 16-17 0.024627739788224395 0.0 0.0 18-19 0.03582564529524719 0.0 0.0 20-21 0.04664093444098219 0.0 0.0 22-23 0.05944582866541372 0.0 0.0 24-25 0.0730924788846784 0.0 0.0 26-27 0.0878487165516777 0.0 0.0 28-29 0.10385483433221712 0.0 0.0 30-31 0.1242227786314374 0.0 0.0 32-33 0.1432133040300216 0.0 0.0 34-35 0.1642954655369791 0.0 0.0 36-37 0.18769883296908257 0.0 0.0 38-39 0.21468604031858168 0.0 0.0 40-41 0.2453846263725724 0.0 0.0 42-43 0.27929718986138063 0.0 0.0 44-45 0.31560748254759236 0.0 0.0 46-47 0.35518276818345995 0.0 0.0 48-49 0.399196403610266 0.0 2.550775741918632E-5 50-51 0.4478779586447831 0.0 2.550775741918632E-5 52-53 0.4979369325799362 0.0 2.550775741918632E-5 54-55 0.550954806375715 0.0 2.550775741918632E-5 56-57 0.6069570877895385 0.0 2.550775741918632E-5 58-59 0.6667600250588208 0.0 2.550775741918632E-5 60-61 0.7315624827822638 0.0 2.550775741918632E-5 62-63 0.8011731527792232 0.0 2.550775741918632E-5 64-65 0.8712812240458568 0.0 2.550775741918632E-5 66-67 0.9491691613253423 0.0 2.550775741918632E-5 68-69 1.0336125922615587 0.0 2.550775741918632E-5 70-71 1.12197146396162 0.0 2.550775741918632E-5 72-73 1.2155976875687435 0.0 2.550775741918632E-5 74-75 1.3147463406571207 0.0 2.550775741918632E-5 76-77 1.419340899954494 0.0 2.550775741918632E-5 78-79 1.5354649656053398 0.0 2.550775741918632E-5 80-81 1.6611927019245094 0.0 2.550775741918632E-5 82-83 1.797416880421674 0.0 2.550775741918632E-5 84-85 1.9425432662581343 0.0 2.550775741918632E-5 86-87 2.0978089856687214 0.0 2.550775741918632E-5 88-89 2.263443608470208 0.0 2.550775741918632E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAGTCG 45 1.5016989E-5 52.866165 1 CGAGGCG 180 0.0 50.222855 1 AGCGCCC 165 0.0 49.02135 1 CGACGGG 160 0.0 47.579548 1 CGCTCGG 50 2.80042E-5 47.579548 1 AGTCGCC 80 4.1673047E-9 47.579548 1 AACCGAG 180 0.0 44.93624 1 CGAGGGG 425 0.0 44.78075 1 CGGACGG 55 4.9167218E-5 43.25413 1 CGTGCGG 45 9.548958E-4 42.29293 1 CGATCGC 45 9.548958E-4 42.29293 1 GCGCATA 45 9.623046E-4 42.226566 4 GATACGT 45 9.63695E-4 42.21418 9 CGACGCC 50 0.0016030591 38.063637 1 CGGAGCG 50 0.0016030591 38.063637 1 GCGCCCC 100 3.031346E-8 38.00391 2 GCGCGTT 50 0.0016178046 37.992764 5 TTGTGTT 24180 0.0 36.639008 1 CGAGCCC 170 0.0 36.38436 1 GGTACGG 80 1.06597545E-5 35.68466 1 >>END_MODULE