##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n01_41442-1.34100000035721.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3146825 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.673464523766015 33.0 33.0 33.0 33.0 33.0 2 32.71875143994343 33.0 33.0 33.0 33.0 33.0 3 32.559149619060484 33.0 33.0 33.0 33.0 33.0 4 36.60063301899534 37.0 37.0 37.0 37.0 37.0 5 36.674529724404756 37.0 37.0 37.0 37.0 37.0 6 36.56573308016811 37.0 37.0 37.0 37.0 37.0 7 36.44065621698061 37.0 37.0 37.0 37.0 37.0 8 36.399608494275974 37.0 37.0 37.0 37.0 37.0 9 36.44284350098909 37.0 37.0 37.0 37.0 37.0 10-11 36.376763881054714 37.0 37.0 37.0 37.0 37.0 12-13 36.36327552374218 37.0 37.0 37.0 37.0 37.0 14-15 38.19477854663033 40.0 37.0 40.0 37.0 40.0 16-17 38.2352779388749 40.0 37.0 40.0 37.0 40.0 18-19 38.21264321975325 40.0 37.0 40.0 37.0 40.0 20-21 38.19444265251484 40.0 37.0 40.0 37.0 40.0 22-23 37.96572640677508 40.0 37.0 40.0 33.0 40.0 24-25 38.023132363572806 40.0 37.0 40.0 35.0 40.0 26-27 37.97002740857849 40.0 37.0 40.0 35.0 40.0 28-29 37.74631175867739 40.0 37.0 40.0 33.0 40.0 30-31 37.71997441866007 40.0 37.0 40.0 33.0 40.0 32-33 37.60287893352824 40.0 37.0 40.0 33.0 40.0 34-35 37.59239407974705 40.0 37.0 40.0 33.0 40.0 36-37 37.505303440769666 40.0 37.0 40.0 33.0 40.0 38-39 37.22710176129908 40.0 37.0 40.0 33.0 40.0 40-41 37.083267579226685 38.5 37.0 40.0 33.0 40.0 42-43 36.850486283793984 37.0 37.0 40.0 33.0 40.0 44-45 36.65307540139665 37.0 37.0 40.0 33.0 40.0 46-47 36.78782201107465 37.0 37.0 40.0 33.0 40.0 48-49 36.39475423641289 37.0 37.0 40.0 33.0 40.0 50-51 36.313795333391596 37.0 37.0 40.0 33.0 40.0 52-53 36.304239829034024 37.0 37.0 40.0 33.0 40.0 54-55 35.91306094237843 37.0 37.0 40.0 30.0 40.0 56-57 35.76021164189302 37.0 37.0 40.0 27.0 40.0 58-59 35.601023412487194 37.0 37.0 40.0 27.0 40.0 60-61 35.197104859660136 37.0 33.0 40.0 27.0 40.0 62-63 34.97136288163466 37.0 33.0 38.5 27.0 40.0 64-65 34.69288949973386 37.0 33.0 37.0 27.0 40.0 66-67 33.95851358114925 37.0 33.0 37.0 27.0 40.0 68-69 33.89922604529963 37.0 33.0 37.0 27.0 40.0 70-71 33.57238565856061 37.0 33.0 37.0 27.0 38.5 72-73 32.709540886449034 37.0 33.0 37.0 22.0 37.0 74-75 31.80053482478371 37.0 33.0 37.0 18.5 37.0 76-77 29.295189436972187 33.0 27.0 35.0 15.0 37.0 78-79 31.240228007594958 33.0 33.0 37.0 15.0 37.0 80-81 31.48770379668396 37.0 33.0 37.0 15.0 37.0 82-83 31.39322189826253 37.0 33.0 37.0 15.0 37.0 84-85 31.23109737592653 33.0 33.0 37.0 15.0 37.0 86-87 31.08189905698601 33.0 33.0 37.0 15.0 37.0 88-89 30.97530812803381 33.0 33.0 37.0 15.0 37.0 90-91 30.84518554415959 33.0 33.0 37.0 15.0 37.0 92-93 30.681758915732523 33.0 33.0 37.0 15.0 37.0 94-95 30.567928467582405 33.0 33.0 37.0 15.0 37.0 96-97 30.434713401603204 33.0 30.0 37.0 15.0 37.0 98-99 30.270035670874613 33.0 27.0 37.0 15.0 37.0 100-101 28.74884923692929 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 0.16598641291429317 2306 2 0.12720092312115838 2306 3 0.3453999115592836 2306 4 0.2623665679433458 2306 5 0.19870790739835797 2306 6 0.18196501959941713 2306 7 0.20785476158371807 2306 8 0.14976930462128024 2306 9 0.16045342483231195 2306 10-11 0.21478919022153775 2306 12-13 0.22757738582554765 2306 14-15 0.4204518769955783 2306 16-17 0.3932706501265457 2306 18-19 0.5009315910647132 2306 20-21 0.43341918768217624 2306 22-23 0.5901070679323794 2306 24-25 0.49198161567147025 2306 26-27 0.5289554628413029 2306 28-29 0.5072137852827581 2306 30-31 0.508722054110514 2306 32-33 0.5310257090887944 2306 34-35 0.5425517549173478 2306 36-37 0.5867214944823473 2306 38-39 0.5863116987021968 2306 40-41 0.6263969081438958 2306 42-43 0.7214189070641126 2306 44-45 0.7426027002369651 2306 46-47 0.49008792374004173 2306 48-49 0.33151641370442064 2306 50-51 0.35494002497877375 2306 52-53 0.30156995394860076 2306 54-55 0.2899865689127523 2306 56-57 0.4975663906366421 2306 58-59 0.44305474174603887 2306 60-61 0.32804069610521225 2306 62-63 0.30387358482050786 2306 64-65 0.25712224031143904 2306 66-67 0.12152406549664363 2306 68-69 0.47764311054469744 2306 70-71 0.5484518628149644 2306 72-73 0.3935990511309413 2306 74-75 0.33976847647434383 2306 76-77 0.19224241220563698 2306 78-79 0.6183875318643395 2306 80-81 0.6884475863315345 2306 82-83 0.539436189124519 2306 84-85 0.7740728411971247 2306 86-87 0.6353526492032877 2306 88-89 0.7328720043307726 2306 90-91 0.5856540761365991 2306 92-93 0.7540486998425457 2306 94-95 0.6133997733807774 2306 96-97 0.6816782007986326 2306 98-99 0.689180804009645 2306 100-101 0.6748562780600373 2307 1 0.11721228265867722 2307 2 0.16454158762231685 2307 3 0.338552038044746 2307 4 0.21430635198188952 2307 5 0.16823831149665835 2307 6 0.17861085822056566 2307 7 0.19012953398194554 2307 8 0.14781787550935377 2307 9 0.2063323521927245 2307 10-11 0.19095738120454087 2307 12-13 0.19160247454200174 2307 14-15 0.4532036220930067 2307 16-17 0.4496441176111219 2307 18-19 0.44350265215429374 2307 20-21 0.3921397315879105 2307 22-23 0.4031765476685294 2307 24-25 0.3730826597354735 2307 26-27 0.4577238323504105 2307 28-29 0.5095711283808342 2307 30-31 0.3819239359543758 2307 32-33 0.440333497408929 2307 34-35 0.35612183021581956 2307 36-37 0.40533269827873397 2307 38-39 0.4909972959803852 2307 40-41 0.48606255669843534 2307 42-43 0.535158192408332 2307 44-45 0.5382896502448986 2307 46-47 0.3221402039404708 2307 48-49 0.3178741864819514 2307 50-51 0.24897213876397473 2307 52-53 0.32838955607684284 2307 54-55 0.3785143179343251 2307 56-57 0.3752411213977922 2307 58-59 0.3187349803892445 2307 60-61 0.34678973954562053 2307 62-63 0.2878287650719855 2307 64-65 0.2458228117280683 2307 66-67 0.148659272878767 2307 68-69 0.5754385317969053 2307 70-71 0.4691888391074954 2307 72-73 0.21802613728641518 2307 74-75 0.3204034324179439 2307 76-77 0.14358302157094016 2307 78-79 0.5390940883733641 2307 80-81 0.6600571826324853 2307 82-83 0.7414750751140211 2307 84-85 0.6925965881159364 2307 86-87 0.6772819479227081 2307 88-89 0.7040350001851223 2307 90-91 0.708894125302578 2307 92-93 0.7068125748286427 2307 94-95 0.6925539513003862 2307 96-97 0.7638306286300747 2307 98-99 0.5824270417003277 2307 100-101 0.6410628455011711 2201 1 0.17888013374157197 2201 2 0.1314069439027179 2201 3 0.28897234723942944 2201 4 0.16626692622659078 2201 5 0.09284540657729679 2201 6 0.11975317596455426 2201 7 0.16238275693154947 2201 8 0.23673364241955142 2201 9 0.21999784051002536 2201 10-11 0.16868790183200133 2201 12-13 0.1969706514979137 2201 14-15 0.2923461929904576 2201 16-17 0.32574196390395826 2201 18-19 0.32806533783118397 2201 20-21 0.33284614555337555 2201 22-23 0.3972162729060216 2201 24-25 0.30321942051290307 2201 26-27 0.377278613186526 2201 28-29 0.4444670914105089 2201 30-31 0.40450886438888034 2201 32-33 0.3959365858810955 2201 34-35 0.35637920289659064 2201 36-37 0.4413959800244669 2201 38-39 0.5032585271831209 2201 40-41 0.3547190977783714 2201 42-43 0.42265141889878066 2201 44-45 0.39208856532516023 2201 46-47 0.39168576039410397 2201 48-49 0.2546392658654142 2201 50-51 0.20051550618477876 2201 52-53 0.2253249101152619 2201 54-55 0.28116895457225155 2201 56-57 0.3637654932090797 2201 58-59 0.3213291414792252 2201 60-61 0.3301084986303877 2201 62-63 0.30752319395861605 2201 64-65 0.31151993926362564 2201 66-67 0.20388627128125592 2201 68-69 0.37526455084298505 2201 70-71 0.1875221131510756 2201 72-73 0.11415595040787707 2201 74-75 0.10651027144957581 2201 76-77 0.02421446571213437 2201 78-79 0.48314138339559065 2201 80-81 0.4822502195887566 2201 82-83 0.49202684210032643 2201 84-85 0.5253244489775 2201 86-87 0.5617479223608264 2201 88-89 0.47073588944869016 2201 90-91 0.5021194575251258 2201 92-93 0.3868945270554427 2201 94-95 0.32461567110584255 2201 96-97 0.34186352699723344 2201 98-99 0.27937025507808144 2201 100-101 0.27406321861296234 1101 1 0.1352852479825728 1101 2 0.09732011932602802 1101 3 0.3139462448568935 1101 4 0.2264474147223794 1101 5 0.1442673057976691 1101 6 0.13660264018919577 1101 7 0.16773664470191108 1101 8 0.12668974830433655 1101 9 0.20119853201136095 1101 10-11 0.13644876567897768 1101 12-13 0.0981328100099077 1101 14-15 0.29267552634374994 1101 16-17 0.23970074656812557 1101 18-19 0.22363327089590967 1101 20-21 0.2069887396572625 1101 22-23 0.21424519255279506 1101 24-25 0.23302658941896226 1101 26-27 0.19552402641352273 1101 28-29 0.17599477210915637 1101 30-31 0.2501606688872684 1101 32-33 0.1660735026859328 1101 34-35 0.30770901379969473 1101 36-37 0.2767012557572528 1101 38-39 0.35393949215945497 1101 40-41 0.42159252174472783 1101 42-43 0.4263314147928057 1101 44-45 0.3687229232164171 1101 46-47 0.4286456154479481 1101 48-49 0.3583380643579801 1101 50-51 0.29280797065271713 1101 52-53 0.3821727984090799 1101 54-55 0.2621752867544558 1101 56-57 0.41949825416256914 1101 58-59 0.35036829908333544 1101 60-61 0.3415844993259114 1101 62-63 0.311384142244556 1101 64-65 0.14068048302227965 1101 66-67 0.0735938045102742 1101 68-69 0.17100327006524196 1101 70-71 0.0713637554618245 1101 72-73 0.1060026468834323 1101 74-75 -0.043908378359347466 1101 76-77 -0.032027593973531054 1101 78-79 0.28387488214134393 1101 80-81 0.26525597700035775 1101 82-83 0.21051229690031548 1101 84-85 0.33073153675108813 1101 86-87 0.3598423949726133 1101 88-89 0.32349081558967896 1101 90-91 0.39530515235023245 1101 92-93 0.2792470183668492 1101 94-95 0.20498714004610363 1101 96-97 0.2728500942431751 1101 98-99 0.19501510277950018 1101 100-101 0.21689468268067102 2304 1 0.0709663517711121 2304 2 -0.0022937170969470344 2304 3 0.22345999667283678 2304 4 0.134390363533484 2304 5 0.07774803124528518 2304 6 0.11074835145021211 2304 7 0.11962006415507176 2304 8 0.06765984927624658 2304 9 0.057647673815210965 2304 10-11 0.09824729122482978 2304 12-13 0.03355156461395126 2304 14-15 0.1582891749601245 2304 16-17 0.1981976856186165 2304 18-19 0.24840931781181297 2304 20-21 0.25745697847511906 2304 22-23 0.29193574530144417 2304 24-25 0.28384198824224427 2304 26-27 0.1816355098457123 2304 28-29 0.22467704553454126 2304 30-31 0.282741976362864 2304 32-33 0.28152470174006794 2304 34-35 0.1804172992211832 2304 36-37 0.17254666992956658 2304 38-39 0.23654320944439178 2304 40-41 0.2597204098784758 2304 42-43 0.29545240628616654 2304 44-45 0.3649811356394608 2304 46-47 0.377253941989359 2304 48-49 0.24678831983293747 2304 50-51 0.2593199241970865 2304 52-53 0.24581698613226877 2304 54-55 0.2801775984371986 2304 56-57 0.2950487210688735 2304 58-59 0.2162069863936722 2304 60-61 0.2877881020456883 2304 62-63 0.3678023853186474 2304 64-65 0.23727099396122497 2304 66-67 0.14079477934874518 2304 68-69 0.1372700403860918 2304 70-71 0.23153729940817414 2304 72-73 0.04871905125578735 2304 74-75 -0.13679096334300667 2304 76-77 -0.051418937594839065 2304 78-79 0.13018623066004764 2304 80-81 0.2612654005642554 2304 82-83 0.21093282375265687 2304 84-85 0.20718129621599957 2304 86-87 0.15730208357424402 2304 88-89 0.26220606176915595 2304 90-91 0.15294200621052667 2304 92-93 0.2784521324750244 2304 94-95 0.22125553290299393 2304 96-97 0.14664649779406957 2304 98-99 0.15974218829875042 2304 100-101 0.14088498320726828 2202 1 0.10570966727257058 2202 2 0.07873270181343628 2202 3 0.2686888963369398 2202 4 0.16419156206127639 2202 5 0.07262305471635244 2202 6 0.10303879624073886 2202 7 0.13898354108837907 2202 8 0.10870138784918026 2202 9 0.04453455738521939 2202 10-11 0.09821108748737117 2202 12-13 0.0939301943454467 2202 14-15 0.105785778558662 2202 16-17 0.08392948587707139 2202 18-19 0.020662037080668938 2202 20-21 0.06545935442209583 2202 22-23 0.13157896053696305 2202 24-25 0.08215035326919207 2202 26-27 0.01900034411236362 2202 28-29 0.15853158996265648 2202 30-31 0.18899515953045665 2202 32-33 0.18789263234670273 2202 34-35 0.12635898297724424 2202 36-37 0.11070700462391159 2202 38-39 0.23944073568596025 2202 40-41 0.1509484283804241 2202 42-43 0.13803119236871453 2202 44-45 0.21254867401849253 2202 46-47 0.3622428906450992 2202 48-49 0.340648043236385 2202 50-51 0.3310499596767471 2202 52-53 0.20993846869372135 2202 54-55 0.3368677423071702 2202 56-57 0.39588656146070633 2202 58-59 0.4453054642412795 2202 60-61 0.32258480271932655 2202 62-63 0.36947082535482423 2202 64-65 0.18711899962619327 2202 66-67 0.05497903550920569 2202 68-69 0.04511373351094505 2202 70-71 0.08570864494768671 2202 72-73 0.04500786135552204 2202 74-75 0.1337896341536613 2202 76-77 -0.20959368014945312 2202 78-79 0.08154804344868083 2202 80-81 0.2948219698244614 2202 82-83 0.4651867629033788 2202 84-85 0.3294130909518529 2202 86-87 0.5096074025782613 2202 88-89 0.511846172073966 2202 90-91 0.41629719678331867 2202 92-93 0.43669682801386855 2202 94-95 0.4380343084312308 2202 96-97 0.4485175560354513 2202 98-99 0.3782814472634719 2202 100-101 0.3527388231965638 1102 1 0.08185891309478421 1102 2 0.0734939103904324 1102 3 0.2866361038919649 1102 4 0.16606636097874627 1102 5 0.06283624951564803 1102 6 0.047749379332771014 1102 7 0.041091832312666554 1102 8 0.14645877349235548 1102 9 0.12092108059724183 1102 10-11 0.099192843387371 1102 12-13 0.11353928989986883 1102 14-15 0.19329394014385315 1102 16-17 0.1584252418527754 1102 18-19 0.17076364290212354 1102 20-21 0.19095023237209574 1102 22-23 0.1853596670226949 1102 24-25 0.11509772642159533 1102 26-27 0.11493977805319844 1102 28-29 0.17695784708335083 1102 30-31 0.22901827219344995 1102 32-33 0.15125243760418527 1102 34-35 0.1923371193916381 1102 36-37 0.1834947419013062 1102 38-39 0.2786127960958851 1102 40-41 0.20145917801610835 1102 42-43 0.14542334116032407 1102 44-45 0.3129420860815486 1102 46-47 0.2677258199208197 1102 48-49 0.2511574444362594 1102 50-51 0.2613825501875411 1102 52-53 0.293044697047776 1102 54-55 0.3077545399377044 1102 56-57 0.3788099229929074 1102 58-59 0.24904122286653774 1102 60-61 0.29550844813274324 1102 62-63 0.3883282270846067 1102 64-65 0.3423582641845755 1102 66-67 0.22068582878689824 1102 68-69 -0.032849513333246705 1102 70-71 -0.04567040272542755 1102 72-73 -0.24115505807898785 1102 74-75 -0.28656867126468555 1102 76-77 -0.16903415025675628 1102 78-79 0.016701883415368712 1102 80-81 0.06831787247212517 1102 82-83 0.11244009377641007 1102 84-85 -0.10162506268466132 1102 86-87 0.046907621166806024 1102 88-89 0.09142162770333329 1102 90-91 -0.007355905092190085 1102 92-93 0.09807620688174623 1102 94-95 0.06422838383008411 1102 96-97 -0.08797892383737249 1102 98-99 -0.04515696943631653 1102 100-101 -0.16151083141461342 2305 1 0.15595734578297282 2305 2 0.026252617730641248 2305 3 0.27776740750842066 2305 4 0.12878059106109419 2305 5 0.04928680970091648 2305 6 0.06514414069064145 2305 7 5.776423984542589E-4 2305 8 -0.03474586055325091 2305 9 0.008252864722713582 2305 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98-99 0.08691622153197542 1213 100-101 0.23442549913779587 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 11.0 10 29.0 11 21.0 12 47.0 13 148.0 14 442.0 15 1240.0 16 2645.0 17 4643.0 18 5793.0 19 6741.0 20 8568.0 21 11346.0 22 15088.0 23 19654.0 24 25566.0 25 32544.0 26 43770.0 27 63026.0 28 99989.0 29 124116.0 30 83640.0 31 67478.0 32 87474.0 33 122936.0 34 189170.0 35 328726.0 36 643383.0 37 949136.0 38 209276.0 39 178.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.95400343969129 23.623606226331432 21.85926041226002 13.563129921717259 2 27.678819127215526 29.15163696741954 30.52988329506725 12.639660610297682 3 34.31083711359862 17.91329355779238 32.51683840061014 15.259030927998857 4 27.181789893014287 17.45311130754348 30.83212629139184 24.532972508050392 5 36.72625582928825 16.158032302400038 31.519928817141086 15.595783051170626 6 32.22015841363914 17.039778189127137 35.062960285367 15.677103111866723 7 30.08521287329292 26.475987701890002 26.435883787627212 17.002915637189865 8 26.891231638238544 33.91396089709469 22.8121678199455 16.382639644721266 9 35.09565991118031 23.3978692809419 23.81330388566253 17.693166922215248 10-11 26.506889570952787 29.998142570209335 27.015561575386464 16.47940628345141 12-13 21.78819921667077 28.675490375219464 27.010955486879634 22.52535492123013 14-15 20.947033915136686 29.291110881602883 27.785577526554544 21.976277676705884 16-17 24.703359735606522 25.55534546725668 28.444511531464258 21.29678326567254 18-19 19.495650377761713 25.57441230446561 33.12419661086969 21.80574070690299 20-21 24.272703439180763 25.858937182715913 32.56420360204333 17.304155776059996 22-23 19.551500321752876 34.79894814614731 28.16163911243873 17.487912419661086 24-25 19.910783090893204 25.231034455363737 32.73864927347406 22.119533180269002 26-27 19.71350801419018 25.381221609276274 28.04487507962392 26.86039529690963 28-29 23.833283229874457 30.263961545966055 27.76566326280927 18.13709196135021 30-31 18.75951157118683 26.419216194100404 32.08030316271162 22.740969072001143 32-33 18.49481620363382 31.017438211530667 28.231090066972264 22.256655517863244 34-35 18.88811738816108 26.37976373010892 32.873420034479196 21.8586988472508 36-37 23.480968389702397 27.01423450111558 28.160767588314574 21.34402952086745 38-39 24.079372506897865 31.316882268775576 27.267969203708837 17.335776020617722 40-41 23.820652224861753 30.992383247370714 27.13807157621511 18.04889295155242 42-43 19.00735444830833 31.028999908796322 32.179993472740435 17.783652170154916 44-45 18.64026007248599 26.541566631657727 32.62474915112582 22.193424144730464 46-47 23.592260747090933 26.200491179364366 27.89232061910972 22.31492745443498 48-49 19.335844955278617 31.379713064936176 31.47988133619135 17.804560643593856 50-51 23.90062325769411 26.608562261575013 31.120951780472954 18.369862700257922 52-53 18.978899921806835 26.56651289154381 26.77755975357633 27.67702743307302 54-55 23.86330339431097 26.558482790094 31.13196454097701 18.44624927461802 56-57 19.29618066530039 26.57117654564821 31.01474382596428 23.11789896308712 58-59 19.172358555632186 26.522523352901356 31.661909741314375 22.643208350152083 60-61 23.28829898885251 26.76651287987549 31.653055727915756 18.29213240335624 62-63 18.85291225759788 26.809145609384206 31.320124293950492 23.017817839067426 64-65 23.484032642644717 26.657783773862842 31.141022676801743 18.717160906690694 66-67 19.441165270978395 35.2815914724114 26.428840240575845 18.84840301603436 68-69 19.513499501996225 35.71110600066337 26.312987305870312 18.462407191470096 70-71 18.82655244074626 36.5817283027648 26.27092865139289 18.320790605096047 72-73 18.789387613691083 36.22633803063123 26.169315684164857 18.814958671512823 74-75 19.442682103501895 35.02076242471555 26.136116574721207 19.40043889706135 76-77 19.839256877338133 33.77906855700062 26.584072759855893 19.79760180580536 78-79 19.645701981256135 33.36869162405794 27.108017591742843 19.87758880294308 80-81 19.03989217977643 33.34428124273549 27.119036189911427 20.49679038757665 82-83 18.56044139032104 32.705120529403985 27.32716958011735 21.407268500157624 84-85 18.719141766095223 32.08139264385467 27.781277092321734 21.418188497728377 86-87 18.817991600216182 31.95255007285332 28.378073223905297 20.851385103025198 88-89 19.179094456499886 32.34326841403948 27.850353227596802 20.62728390186383 90-91 19.702300448964998 31.74894513091501 27.442965552862645 21.105788867257342 92-93 19.075563596522247 32.287094719942374 27.48562789335343 21.151713790181944 94-95 18.88173781312489 32.57697145231081 27.45564385269363 21.085646881870677 96-97 19.342935300516018 32.98432115223606 26.78124946360833 20.891494083639593 98-99 19.840277979764075 32.08554279849852 26.50194757172209 21.572231650015315 100-101 19.707218088704224 32.1345724987413 26.16422067222361 21.993988740330874 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1070.0 1 689.0 2 323.5 3 473.5 4 525.5 5 435.0 6 422.0 7 427.5 8 454.0 9 456.5 10 474.5 11 511.0 12 491.0 13 475.5 14 491.5 15 507.5 16 535.0 17 572.5 18 595.5 19 652.5 20 760.5 21 854.0 22 978.5 23 1197.5 24 1561.0 25 2174.0 26 3533.5 27 5889.5 28 8254.0 29 10445.5 30 12757.0 31 15028.0 32 18550.0 33 27969.5 34 45673.0 35 77110.0 36 117764.5 37 162184.5 38 211339.5 39 243570.0 40 254663.5 41 242074.5 42 218937.5 43 213093.0 44 203361.0 45 184314.0 46 173731.0 47 153996.5 48 119627.0 49 92462.0 50 72697.0 51 58947.0 52 49050.0 53 37842.0 54 28355.0 55 20602.5 56 14141.0 57 9167.0 58 6158.0 59 4526.0 60 3302.5 61 2392.0 62 1651.5 63 1137.5 64 817.5 65 571.0 66 412.5 67 310.0 68 233.0 69 168.5 70 123.0 71 84.0 72 53.0 73 43.0 74 40.0 75 30.5 76 19.0 77 13.5 78 8.5 79 7.5 80 5.5 81 5.0 82 5.5 83 1.5 84 0.0 85 1.0 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16727971844637055 2 0.0 3 0.0 4 0.022371755658481166 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.1122321705210809E-4 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.588903100744401E-5 28-29 9.533418604466407E-5 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0010804541085061928 38-39 5.561160852605404E-4 40-41 3.6544771317121227E-4 42-43 6.991173643275364E-4 44-45 3.177806201488802E-4 46-47 0.003590921007682347 48-49 0.0038133674417865624 50-51 0.004226482247980107 52-53 0.004147037092942887 54-55 0.006578058837081821 56-57 0.010216646937786499 58-59 0.017001263177965093 60-61 0.004846154457270424 62-63 0.00289180364335481 64-65 0.010407315309875827 66-67 0.022467089844525834 68-69 0.024294328410381892 70-71 0.02475511030959777 72-73 0.021926862790272734 74-75 0.024055992945270232 76-77 0.02381765748015857 78-79 0.014697353681885711 80-81 0.0033525855425706862 82-83 0.001302900542610409 84-85 0.00562471697663518 86-87 0.013807567945468845 88-89 0.015332914922183472 90-91 0.022244643410421615 92-93 0.022673647247622605 94-95 0.024024214883255346 96-97 0.02551778379795508 98-99 0.03128550205365726 100-101 0.023166207208853366 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3146825.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.245267538369205 #Duplication Level Percentage of deduplicated Percentage of total 1 86.56260083742337 33.1060982784432 2 5.824551624452195 4.455230703364343 3 2.1238045698202686 2.436764219159619 4 1.1853773261627794 1.813402918920489 5 0.7342077180658216 1.4039985303081448 6 0.47569905422947933 1.0915942558053382 7 0.3900099127344668 1.0441223418601997 8 0.3063432480054988 0.9372943582834631 9 0.2727484416024574 0.9388203407783329 >10 1.712730981639745 13.157639535284021 >50 0.2062985910402809 5.426566037954057 >100 0.17202122670269096 14.567763639250938 >500 0.02634290281054278 6.82975819887276 >1k 0.006847380407655766 4.097534486880695 >5k 3.3294792319800166E-4 0.7394449357098489 >10k+ 8.323697918143487E-5 7.953967219124565 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT 249856 7.939939462791862 TruSeq Adapter, Index 6 (97% over 36bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7148 0.22714958728241957 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATATCGTAT 5494 0.17458867270979478 TruSeq Adapter, Index 6 (97% over 36bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 5367 0.17055285883390403 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 5219 0.1658497056557006 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 4768 0.1515177996869861 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 4424 0.1405861463538646 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 3578 0.11370190588926934 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3415 0.1085220817808426 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.005656495038650068 0.0 0.0 2 0.006069609844843612 0.0 0.0 3 0.006768727209171149 0.0 0.0 4 0.007181842015364693 0.0 0.0 5 0.008484742557975102 0.0 0.0 6 0.009438084418421743 0.0 0.0 7 0.01093165333312148 0.0 0.0 8 0.012584112557895656 0.0 0.0 9 0.01445901821677405 0.0 0.0 10-11 0.024230772286352117 0.0 0.0 12-13 0.03333518705361753 0.0 0.0 14-15 0.045108959030133546 0.0 0.0 16-17 0.059647422401944816 0.0 0.0 18-19 0.07536167406830693 0.0 0.0 20-21 0.08619799321538377 0.0 0.0 22-23 0.09678008786634147 0.0 0.0 24-25 0.10907819786610314 0.0 0.0 26-27 0.12196420201314023 0.0 0.0 28-29 0.13521565387334855 0.0 0.0 30-31 0.2298983896467074 0.0 0.0 32-33 0.24815488627426055 0.0 0.0 34-35 0.2614063381344689 0.0 0.0 36-37 0.27400633972337196 0.0 0.0 38-39 0.2889102508083544 0.0 0.0 40-41 0.30511706243594733 0.0 0.0 42-43 0.32149865340462214 0.0 0.0 44-45 0.33973926100116786 0.0 0.0 46-47 0.3592192130162942 0.0 0.0 48-49 0.37852438569033864 0.0 0.0 50-51 0.40024469107751465 0.0 0.0 52-53 0.42395112534062107 0.0 0.0 54-55 0.44854734534014445 0.0 0.0 56-57 0.4763213715411566 0.0 0.0 58-59 0.5064946414242928 0.0 0.0 60-61 0.538495149873285 0.0 0.0 62-63 0.5740548012679447 0.0 0.0 64-65 0.6120136963447285 0.0 0.0 66-67 0.6539448491733733 0.0 0.0 68-69 0.6997688145988417 0.0 0.0 70-71 0.7501052648304243 0.0 0.0 72-73 0.8030475161472277 0.0 0.0 74-75 0.8618210418437632 0.0 0.0 76-77 0.9234387040906311 0.0 0.0 78-79 0.9910465310273053 0.0 0.0 80-81 1.0660268683514336 0.0 0.0 82-83 1.1474740412955917 0.0 0.0 84-85 1.2364685039682854 0.0 0.0 86-87 1.334615048501267 0.0 0.0 88-89 1.438179752607787 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 34475 0.0 78.740875 3 CGGAAGA 34830 0.0 78.702156 4 GAAGAGC 34810 0.0 78.64424 6 GATCGGA 34675 0.0 78.39512 1 ATCGGAA 34960 0.0 77.702866 2 GGAAGAG 35895 0.0 76.39937 5 GAGCACA 43585 0.0 62.77805 9 AGAGCAC 43690 0.0 62.659786 8 AAGAGCA 44110 0.0 62.020092 7 GCGGAAG 310 0.0 50.57275 3 GAGCGGA 330 0.0 49.023716 1 GCCGTCT 29980 0.0 44.898205 50-51 CGTCTTC 30085 0.0 44.839077 52-53 ATGCCGT 29945 0.0 44.815506 48-49 TCGTATG 30485 0.0 44.589985 44-45 TCTCGTA 29840 0.0 44.503296 42-43 CTGCTTG 30260 0.0 44.494843 58-59 AATCTCG 29760 0.0 44.486923 40-41 GTATGCC 30435 0.0 44.43695 46-47 TGCCGTC 30025 0.0 44.37217 50-51 >>END_MODULE