FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n01_41442-1.34100000035721.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n01_41442-1.34100000035721.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3146825
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT2498567.939939462791862TruSeq Adapter, Index 6 (97% over 36bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71480.22714958728241957No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATATCGTAT54940.17458867270979478TruSeq Adapter, Index 6 (97% over 36bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC53670.17055285883390403No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC52190.1658497056557006No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG47680.1515177996869861No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG44240.1405861463538646No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA35780.11370190588926934No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34150.1085220817808426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG344750.078.7408753
CGGAAGA348300.078.7021564
GAAGAGC348100.078.644246
GATCGGA346750.078.395121
ATCGGAA349600.077.7028662
GGAAGAG358950.076.399375
GAGCACA435850.062.778059
AGAGCAC436900.062.6597868
AAGAGCA441100.062.0200927
GCGGAAG3100.050.572753
GAGCGGA3300.049.0237161
GCCGTCT299800.044.89820550-51
CGTCTTC300850.044.83907752-53
ATGCCGT299450.044.81550648-49
TCGTATG304850.044.58998544-45
TCTCGTA298400.044.50329642-43
CTGCTTG302600.044.49484358-59
AATCTCG297600.044.48692340-41
GTATGCC304350.044.4369546-47
TGCCGTC300250.044.3721750-51
Produced by FastQC (version 0.11.2)