##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n01_40825-1.341000000356f9.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1850838 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.64789625023908 33.0 33.0 33.0 33.0 33.0 2 32.68925265204194 33.0 33.0 33.0 33.0 33.0 3 32.52696886491416 33.0 33.0 33.0 33.0 33.0 4 36.57874595183371 37.0 37.0 37.0 37.0 37.0 5 36.6485170501146 37.0 37.0 37.0 37.0 37.0 6 36.53404998168397 37.0 37.0 37.0 37.0 37.0 7 36.43750884734374 37.0 37.0 37.0 37.0 37.0 8 36.36422366517221 37.0 37.0 37.0 37.0 37.0 9 36.40905849134284 37.0 37.0 37.0 37.0 37.0 10-11 36.33677690862193 37.0 37.0 37.0 37.0 37.0 12-13 36.320265198791034 37.0 37.0 37.0 37.0 37.0 14-15 38.12004805390855 40.0 37.0 40.0 37.0 40.0 16-17 38.06371978530806 40.0 37.0 40.0 37.0 40.0 18-19 37.978955748693295 40.0 37.0 40.0 33.0 40.0 20-21 37.911446868931804 40.0 37.0 40.0 33.0 40.0 22-23 37.7102744810729 40.0 37.0 40.0 33.0 40.0 24-25 37.6516794014387 40.0 37.0 40.0 33.0 40.0 26-27 37.535900224654995 40.0 37.0 40.0 33.0 40.0 28-29 37.316027928970556 40.0 37.0 40.0 33.0 40.0 30-31 37.206862783236566 40.0 37.0 40.0 33.0 40.0 32-33 37.05304029850262 37.0 37.0 40.0 33.0 40.0 34-35 36.86479475783402 37.0 37.0 40.0 33.0 40.0 36-37 36.744162103868625 37.0 37.0 40.0 33.0 40.0 38-39 36.60958063320507 37.0 37.0 40.0 33.0 40.0 40-41 36.49210060523936 37.0 37.0 40.0 33.0 40.0 42-43 36.118246977855435 37.0 37.0 40.0 33.0 40.0 44-45 35.904788803774295 37.0 37.0 40.0 33.0 40.0 46-47 35.88518957358775 37.0 37.0 40.0 33.0 40.0 48-49 35.61217189186736 37.0 35.0 40.0 27.0 40.0 50-51 35.409225712893296 37.0 33.0 40.0 27.0 40.0 52-53 35.20826404039684 37.0 33.0 40.0 27.0 40.0 54-55 34.92089069924002 37.0 33.0 40.0 27.0 40.0 56-57 34.67720891833861 37.0 33.0 38.5 27.0 40.0 58-59 34.42695335842467 37.0 33.0 37.0 27.0 40.0 60-61 34.119022572477974 37.0 33.0 37.0 27.0 40.0 62-63 33.79596242350762 37.0 33.0 37.0 27.0 40.0 64-65 33.486093596522224 37.0 33.0 37.0 27.0 40.0 66-67 33.01314350580656 37.0 33.0 37.0 22.0 37.0 68-69 32.7391973257519 37.0 33.0 37.0 22.0 37.0 70-71 32.38538705170306 33.0 33.0 37.0 22.0 37.0 72-73 31.787934168198404 33.0 33.0 37.0 22.0 37.0 74-75 31.277276293225015 33.0 30.0 37.0 18.5 37.0 76-77 28.553432283106353 30.0 24.5 33.0 15.0 35.0 78-79 31.00946868391507 33.0 30.0 37.0 15.0 37.0 80-81 31.459796049140984 33.0 33.0 37.0 15.0 37.0 82-83 31.384126811746896 33.0 33.0 37.0 15.0 37.0 84-85 31.264857324087792 33.0 33.0 37.0 15.0 37.0 86-87 31.117398173151837 33.0 33.0 37.0 15.0 37.0 88-89 30.98199275139153 33.0 33.0 37.0 15.0 37.0 90-91 30.853507438252294 33.0 33.0 37.0 15.0 37.0 92-93 30.685813939415553 33.0 27.0 37.0 15.0 37.0 94-95 30.536410534039177 33.0 27.0 37.0 15.0 37.0 96-97 30.37404732342863 33.0 27.0 37.0 15.0 37.0 98-99 30.18294064634506 33.0 27.0 37.0 15.0 37.0 100-101 28.378770049026443 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 0.13077844528609006 2306 2 0.11060480350742807 2306 3 0.2842471453165558 2306 4 0.16936797584601493 2306 5 0.13886104237982266 2306 6 0.1090819327437984 2306 7 0.2136900004561184 2306 8 0.17673216205579223 2306 9 0.1782843770718472 2306 10-11 0.19829787325330273 2306 12-13 0.2035571921869348 2306 14-15 0.39453958898619135 2306 16-17 0.43935857511856824 2306 18-19 0.3823532041975781 2306 20-21 0.381718054394689 2306 22-23 0.3766202957231144 2306 24-25 0.3623547331603163 2306 26-27 0.3327246120685743 2306 28-29 0.5336757591384256 2306 30-31 0.5163132833671185 2306 32-33 0.6133848784339406 2306 34-35 0.5527003489632776 2306 36-37 0.4250136765519912 2306 38-39 0.583695417123387 2306 40-41 0.6514391017489487 2306 42-43 0.6122394409565146 2306 44-45 0.6109990602817632 2306 46-47 0.6236451137442529 2306 48-49 0.8458860132164929 2306 50-51 0.6495310572070991 2306 52-53 0.8616792973929535 2306 54-55 0.8064305399534533 2306 56-57 0.835668690694753 2306 58-59 0.7526508533086442 2306 60-61 1.0050038614648003 2306 62-63 1.0565452157407194 2306 64-65 0.9759896026603698 2306 66-67 0.8888697648226369 2306 68-69 0.8394160499301364 2306 70-71 0.8349772480213105 2306 72-73 0.780264921960871 2306 74-75 0.7047760799055247 2306 76-77 0.5144654543623659 2306 78-79 0.8143396337592144 2306 80-81 1.0415390643963427 2306 82-83 0.9710548924691693 2306 84-85 1.1723039676747327 2306 86-87 1.127430117972807 2306 88-89 1.2280474636410155 2306 90-91 1.140305818181485 2306 92-93 0.9662398806050057 2306 94-95 1.0041558977954104 2306 96-97 1.0831102986270906 2306 98-99 1.1225779843491814 2306 100-101 1.089349817839473 2307 1 0.15485933019669318 2307 2 0.17241729336203093 2307 3 0.3925132678033947 2307 4 0.3071487424902699 2307 5 0.1594078503477263 2307 6 0.15856411162746298 2307 7 0.26088013101289675 2307 8 0.3066948269909844 2307 9 0.20498103546759694 2307 10-11 0.22814459078223592 2307 12-13 0.19243157476699935 2307 14-15 0.32894350337062406 2307 16-17 0.36856271212202074 2307 18-19 0.4594883756837902 2307 20-21 0.5174653354280068 2307 22-23 0.47786417949744475 2307 24-25 0.5430307065387936 2307 26-27 0.5103838658310664 2307 28-29 0.4967972952096673 2307 30-31 0.4905319137963531 2307 32-33 0.5777943582938718 2307 34-35 0.662425321715638 2307 36-37 0.6589236549857773 2307 38-39 0.717396690225911 2307 40-41 0.7425334133691948 2307 42-43 0.8521483062951987 2307 44-45 0.7832860069227721 2307 46-47 0.8143398706025451 2307 48-49 0.6820047897394588 2307 50-51 0.5975474289808673 2307 52-53 0.7205488101819526 2307 54-55 0.7727626597039361 2307 56-57 0.899191636686453 2307 58-59 0.816462508341921 2307 60-61 0.8343260329278266 2307 62-63 0.8272318562805694 2307 64-65 0.6555130588206595 2307 66-67 0.6334111928308985 2307 68-69 0.6301335324800519 2307 70-71 0.4082103254973646 2307 72-73 0.3490917661043653 2307 74-75 0.29021354946933187 2307 76-77 0.1493153024712619 2307 78-79 0.6562801295503213 2307 80-81 0.7040233535227962 2307 82-83 0.9263773422059671 2307 84-85 1.0295822182602699 2307 86-87 0.894863042398292 2307 88-89 0.9223038929246918 2307 90-91 0.7348252161825499 2307 92-93 0.6993180523463032 2307 94-95 0.7716073738058924 2307 96-97 0.8353865781067142 2307 98-99 0.6203555044660547 2307 100-101 0.4407300557635523 2201 1 0.19304708414878036 2201 2 0.18209108171588895 2201 3 0.349797052150997 2201 4 0.2532639064664295 2201 5 0.15020377661643636 2201 6 0.20425204615273884 2201 7 0.23311582456874191 2201 8 0.22511522202680823 2201 9 0.1699220205583103 2201 10-11 0.17541273143462632 2201 12-13 0.3022600055567679 2201 14-15 0.31568472022311056 2201 16-17 0.3600733044783695 2201 18-19 0.42298028892072637 2201 20-21 0.3832227356764619 2201 22-23 0.49599597146097096 2201 24-25 0.39598243898718977 2201 26-27 0.5322231283207941 2201 28-29 0.5999528174071571 2201 30-31 0.5729995799786494 2201 32-33 0.614390376352091 2201 34-35 0.6060943357590745 2201 36-37 0.6685162414803401 2201 38-39 0.6079345650610932 2201 40-41 0.7866446410740124 2201 42-43 0.7058059565646175 2201 44-45 0.8264371684458283 2201 46-47 0.801612264258388 2201 48-49 0.8340424853473394 2201 50-51 0.951269457519949 2201 52-53 1.0248296735057636 2201 54-55 0.9248492558790105 2201 56-57 1.1047124209977213 2201 58-59 1.139638730284105 2201 60-61 1.0252408010337177 2201 62-63 0.9858231251515903 2201 64-65 0.7687003753594155 2201 66-67 0.5967835464241631 2201 68-69 0.8263721816041283 2201 70-71 0.7575649501675699 2201 72-73 0.7006610481139433 2201 74-75 0.46700009131543396 2201 76-77 0.259341578675123 2201 78-79 0.8389652462364943 2201 80-81 0.7725866593447073 2201 82-83 0.774854897069936 2201 84-85 0.8471117705778219 2201 86-87 0.8204500623035145 2201 88-89 0.9101076876984386 2201 90-91 0.8512837114896641 2201 92-93 0.8976495560207987 2201 94-95 0.8776087744258092 2201 96-97 0.6789689170163591 2201 98-99 0.6427367108565072 2201 100-101 0.31973076145705903 1101 1 0.17695669801275926 1101 2 0.15756525299514834 1101 3 0.3141072312480304 1101 4 0.24770663399298343 1101 5 0.10402133763155774 1101 6 0.09764423306329206 1101 7 0.09792708319270815 1101 8 0.09463675608466104 1101 9 0.08798582027995394 1101 10-11 0.16469866646692566 1101 12-13 0.09793529067762563 1101 14-15 0.2093352263112962 1101 16-17 0.10324862689778769 1101 18-19 0.2155800412582778 1101 20-21 0.10197474713457666 1101 22-23 0.2143926874825155 1101 24-25 0.22640783440522227 1101 26-27 0.1955537406352832 1101 28-29 0.22720996647565528 1101 30-31 0.10891766807572623 1101 32-33 0.28071659596175635 1101 34-35 0.40548541010682726 1101 36-37 0.3812657422124559 1101 38-39 0.40602658380841916 1101 40-41 0.3809025808720037 1101 42-43 0.4739459519182816 1101 44-45 0.19043830232208592 1101 46-47 0.3846201054816092 1101 48-49 0.33464331188344687 1101 50-51 0.4470621798247052 1101 52-53 0.33157794231969717 1101 54-55 0.5372072282866043 1101 56-57 0.634170398307397 1101 58-59 0.5285184307020572 1101 60-61 0.5585612041991936 1101 62-63 0.3168382641047174 1101 64-65 0.20620993977616564 1101 66-67 0.037480539307601646 1101 68-69 0.19805767161130916 1101 70-71 0.0960293577491953 1101 72-73 0.17673732473711823 1101 74-75 0.03575327501404146 1101 76-77 -0.284524299961749 1101 78-79 0.24972577455462996 1101 80-81 0.3406345805360438 1101 82-83 0.304582489692919 1101 84-85 0.47341556842388144 1101 86-87 0.39307845993601376 1101 88-89 0.44046125674446657 1101 90-91 0.5044503593294358 1101 92-93 0.36845096356192997 1101 94-95 0.2732525156206833 1101 96-97 0.5206546414716406 1101 98-99 0.3727972197793896 1101 100-101 0.2701507432553605 2304 1 0.11699720322898344 2304 2 0.0659288736590824 2304 3 0.27788096722623834 2304 4 0.13356945004748866 2304 5 0.11019799061332236 2304 6 0.15117038483225542 2304 7 0.04019851524049756 2304 8 0.03373292457379762 2304 9 -0.02828259742616268 2304 10-11 0.026191629078091694 2304 12-13 -0.004014611591763639 2304 14-15 0.22265955848547492 2304 16-17 0.22005161925989114 2304 18-19 0.26367998552436234 2304 20-21 0.2899939164636507 2304 22-23 0.37089844534609995 2304 24-25 0.293723029356201 2304 26-27 0.21042096045458436 2304 28-29 0.3728132665517947 2304 30-31 0.26212197607235765 2304 32-33 0.42842385157636187 2304 34-35 0.3482074234358734 2304 36-37 0.23045720785759727 2304 38-39 0.30378564842052214 2304 40-41 0.333330146398616 2304 42-43 0.26258172681603753 2304 44-45 0.3151700337630885 2304 46-47 0.35446609146178076 2304 48-49 0.18991507362486715 2304 50-51 0.3134224407536621 2304 52-53 0.2751402818884259 2304 54-55 0.35322288088372744 2304 56-57 0.4070319034372787 2304 58-59 0.1983320360587939 2304 60-61 0.5199860693780494 2304 62-63 0.45364934181036176 2304 64-65 0.28026521055667075 2304 66-67 0.24658263443205897 2304 68-69 0.12508155869909388 2304 70-71 0.031840757298610356 2304 72-73 0.08075801693672702 2304 74-75 0.16300873262093774 2304 76-77 0.07248502565779802 2304 78-79 0.4061595100618476 2304 80-81 0.3198081485275033 2304 82-83 0.4301072520387663 2304 84-85 0.5261131687253133 2304 86-87 0.4146545524386269 2304 88-89 0.6969108881941004 2304 90-91 0.5747131722440066 2304 92-93 0.6504159375396839 2304 94-95 0.5688458071404945 2304 96-97 0.4086216093108135 2304 98-99 0.5093025459013276 2304 100-101 0.24973446581033087 2202 1 0.0342119779259491 2202 2 0.05819019133608094 2202 3 0.2689371281161286 2202 4 0.16886916349447034 2202 5 0.08706073113401658 2202 6 0.12782302538037982 2202 7 0.039092572329323616 2202 8 0.0976339558145014 2202 9 0.105216967783619 2202 10-11 0.14669782410923204 2202 12-13 0.18855514451718847 2202 14-15 0.13449781652325044 2202 16-17 0.13489547412749658 2202 18-19 0.15842208780803446 2202 20-21 0.027820519789067077 2202 22-23 0.16681769108719635 2202 24-25 0.25708976469443456 2202 26-27 0.326456694958253 2202 28-29 0.2383419889688767 2202 30-31 0.335368079009676 2202 32-33 0.3775723630764176 2202 34-35 0.30795139327485543 2202 36-37 0.2597114404966234 2202 38-39 0.4218209802325745 2202 40-41 0.34618928603922683 2202 42-43 0.25177543899074806 2202 44-45 0.3226351484134753 2202 46-47 0.45237924430367826 2202 48-49 0.41137868255878374 2202 50-51 0.4169346119617856 2202 52-53 0.18012183977971574 2202 54-55 0.2804144862225968 2202 56-57 0.2347718113434567 2202 58-59 0.3064124226427438 2202 60-61 0.4707672327482868 2202 62-63 0.33923093938721394 2202 64-65 0.14020002120407327 2202 66-67 0.24684380037018627 2202 68-69 0.042760304168808716 2202 70-71 0.08049203219692203 2202 72-73 0.17866241561309693 2202 74-75 -0.044154217416121355 2202 76-77 -0.1279467006052819 2202 78-79 0.06804591601001064 2202 80-81 0.001700011238842336 2202 82-83 0.10627972660791585 2202 84-85 0.0068649472799435785 2202 86-87 0.320839078575645 2202 88-89 0.10791968904850435 2202 90-91 0.31994215203355836 2202 92-93 0.16732023116606598 2202 94-95 0.21930292048359235 2202 96-97 0.2896816803946365 2202 98-99 0.07489021303294052 2202 100-101 -0.04494168842639468 1102 1 0.14820724420812326 1102 2 0.044096973940860096 1102 3 0.2831593765899072 1102 4 0.1524111966867494 1102 5 0.10676451515497831 1102 6 0.01154492859933498 1102 7 0.0038751742990399407 1102 8 0.016871399719377678 1102 9 -0.01239609086469784 1102 10-11 0.04721398358180551 1102 12-13 0.047564256150515405 1102 14-15 -0.0164646779744686 1102 16-17 0.07496809717814301 1102 18-19 0.1137919253343469 1102 20-21 0.08795128768922922 1102 22-23 0.19575792051641372 1102 24-25 0.21725010075183349 1102 26-27 0.18281750523122753 1102 28-29 0.2688165280079886 1102 30-31 0.17318656076039218 1102 32-33 0.2500462369198786 1102 34-35 0.20782151839632235 1102 36-37 0.30243145079098355 1102 38-39 0.2575628351017869 1102 40-41 0.1606206211136012 1102 42-43 0.15873155014170948 1102 44-45 0.2448552494163252 1102 46-47 0.2839523757438016 1102 48-49 0.31081425069633184 1102 50-51 0.20087892678917996 1102 52-53 0.24687784376808253 1102 54-55 0.21984757921357811 1102 56-57 0.0800818304836568 1102 58-59 0.09597275363734781 1102 60-61 -0.017873989130322343 1102 62-63 0.37682328274629384 1102 64-65 0.11213892586733465 1102 66-67 0.029218471463984486 1102 68-69 0.08403477964165518 1102 70-71 0.18045631133157514 1102 72-73 0.03941520472357496 1102 74-75 -0.14773734366687918 1102 76-77 -0.1743606210546318 1102 78-79 0.057883048552707095 1102 80-81 0.10050213170816491 1102 82-83 0.31538003895454025 1102 84-85 0.2627731291863391 1102 86-87 0.21570863413213814 1102 88-89 0.17610364869236506 1102 90-91 0.11347133133964604 1102 92-93 0.19514312880328788 1102 94-95 -1.5046589663469945E-4 1102 96-97 0.10127591386564916 1102 98-99 -0.2623667901552196 1102 100-101 -0.06264197489648282 2305 1 0.12463568515630641 2305 2 0.03656043619437099 2305 3 0.21938334089491462 2305 4 0.13693909181975528 2305 5 0.09901647888476361 2305 6 0.11319571830178887 2305 7 0.16738199472156623 2305 8 0.05532040622692591 2305 9 0.07826098614149402 2305 10-11 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74-75 0.20223893492391554 1213 76-77 0.3281633551231913 1213 78-79 -0.04687290590857174 1213 80-81 -0.26283587751275306 1213 82-83 -0.4265196298576548 1213 84-85 -0.2920951400490317 1213 86-87 -0.2547972531317768 1213 88-89 -0.1746715888466035 1213 90-91 -0.10148829438872653 1213 92-93 0.003284348359574807 1213 94-95 0.08952774594911617 1213 96-97 5.841743192007698E-4 1213 98-99 -0.11941264143919739 1213 100-101 0.17929006719881357 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 4.0 9 13.0 10 12.0 11 19.0 12 43.0 13 177.0 14 702.0 15 2196.0 16 4696.0 17 7122.0 18 7213.0 19 6728.0 20 7379.0 21 8231.0 22 9458.0 23 11423.0 24 14010.0 25 17465.0 26 22030.0 27 29571.0 28 43752.0 29 62825.0 30 58456.0 31 56970.0 32 75337.0 33 107414.0 34 163744.0 35 269573.0 36 429464.0 37 386465.0 38 48334.0 39 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.27981585095335 23.11468835961341 21.95095816708165 12.654537622351587 2 33.33073991348784 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25.391633411460106 24.97257998809188 29.178431607736606 20.45735499271141 22-23 23.691997895007557 29.781536795764946 25.74120479480106 20.785260514426437 24-25 23.742920774265492 24.45721883816952 28.483989414524665 23.31587097304032 26-27 22.637130488224923 24.759683116427023 26.5384994630809 26.06468693226715 28-29 24.890792652189255 27.07769511847883 25.901983805164907 22.12952842416701 30-31 22.4326764416983 25.22965273027677 28.48204434964054 23.855626478384387 32-33 22.87558392468709 27.223020059021913 25.842456227935674 24.058939788355328 34-35 23.19506083190425 26.862102463856914 28.377524126908998 21.565312577329838 36-37 22.607082497665896 27.692190903904006 28.690939563954494 21.009787034475604 38-39 22.62168350779598 26.002660433773894 27.36742946122434 24.008226597205788 40-41 25.442773365952863 25.334768001884555 24.854930263870234 24.367528368292348 42-43 23.19345093292004 27.36795980826965 27.353074582545062 22.085514676265248 44-45 22.725989520931797 25.097814091766814 27.296122150437707 24.88007423686368 46-47 25.155097608022608 25.02107231044376 25.418852693743688 24.40497738778995 48-49 22.905002577311233 27.115124224240127 27.266548013092955 22.713325185355686 50-51 25.046933637498064 24.832399163258817 26.414945496464544 23.705721702778572 52-53 22.762398460303206 24.976009777636573 24.46032806088762 27.8012637011726 54-55 25.170338927186254 24.889637665732618 27.3079101021115 22.63211330496963 56-57 22.919569352821554 24.9530369358117 27.015926510403197 25.11146720096355 58-59 22.476493347887644 25.089271239746235 27.313730046364842 25.12050536600128 60-61 24.505754690933575 25.074739929898726 27.405523487492374 23.013981891675325 62-63 22.67619487844209 24.913435356736564 27.450899875161046 24.959469889660305 64-65 25.245029899091747 25.525284070415584 27.026979563572954 22.202706466919715 66-67 23.017901292946217 29.579961360228058 24.758717591904563 22.64341975492117 68-69 22.63294422827497 28.5872514051016 25.487408127972333 23.292396238651104 70-71 22.62528855270905 29.08778838720416 25.220053950540922 23.066869109545863 72-73 22.898666482206433 29.192372209048205 25.184928590111344 22.724032718634014 74-75 23.49812947618578 28.65991053370388 25.226847454696035 22.615112535414305 76-77 23.478671351724092 27.805522199800908 25.242539269420703 23.47326717905429 78-79 23.30356756873595 27.974288863911468 25.401256916825176 23.32088665052741 80-81 23.522198562438508 28.19119362558239 25.33282039450543 22.953787417473674 82-83 23.074653808197628 27.80286430340339 25.49039842685647 23.632083461542514 84-85 22.83876172305784 28.03810706352564 25.348010761141666 23.775120452274855 86-87 22.590139112842216 28.2170925502436 25.417381756245593 23.775386580668588 88-89 22.550267786844806 28.405859049714703 25.63018126070296 23.413691902737526 90-91 22.98353920028707 27.881328980102694 25.25515063689371 23.879981182716527 92-93 22.64811883553112 27.69623486089397 24.855695157269448 24.799951146305467 94-95 22.849364595126985 28.131696205404793 24.492986632583854 24.525952566884367 96-97 22.739109487043198 28.828328317644047 24.219793926642943 24.21276826866981 98-99 22.869890083163874 27.71066267624189 24.766485836469148 24.65296140412509 100-101 23.544805630470513 27.858231985350447 24.314436929980925 24.282525454198115 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3386.0 1 2007.5 2 703.0 3 1971.0 4 2502.5 5 1491.5 6 1284.5 7 1510.0 8 1610.0 9 1535.0 10 1414.0 11 1275.5 12 1214.0 13 1194.0 14 1108.5 15 1097.5 16 1148.0 17 1215.5 18 1252.5 19 1284.0 20 1333.0 21 1403.0 22 1530.5 23 1633.5 24 1780.0 25 1946.5 26 2150.0 27 2553.0 28 2969.5 29 3518.5 30 4291.5 31 5133.5 32 6330.0 33 8236.0 34 11172.0 35 16251.5 36 22989.0 37 31752.0 38 40984.0 39 47265.5 40 54911.5 41 59666.5 42 64359.5 43 75115.5 44 80520.5 45 83277.5 46 93180.0 47 99067.0 48 95409.0 49 92098.0 50 88993.0 51 86035.5 52 83342.5 53 80741.5 54 75776.5 55 66499.5 56 57426.5 57 51019.0 58 47025.5 59 40601.5 60 31510.5 61 23278.5 62 16332.0 63 11502.0 64 8721.5 65 7234.5 66 6730.5 67 6198.0 68 5222.5 69 4022.5 70 2798.5 71 1942.0 72 1658.0 73 1420.0 74 944.0 75 562.5 76 431.5 77 339.5 78 271.5 79 223.5 80 170.5 81 129.0 82 100.0 83 76.0 84 51.0 85 36.5 86 27.0 87 24.5 88 25.0 89 19.0 90 11.0 91 8.5 92 5.5 93 4.5 94 4.5 95 2.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16138635580207453 2 0.0 3 5.402958011452109E-5 4 0.02031512212305993 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.0805916022904218E-4 12-13 5.402958011452109E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 2.7014790057260546E-5 28-29 5.402958011452109E-5 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.001188650762519464 38-39 6.483549613742532E-4 40-41 2.1611832045808436E-4 42-43 5.132810110879504E-4 44-45 2.97162690629866E-4 46-47 0.0036740114477874348 48-49 0.003890129768245519 50-51 0.004673558679906075 52-53 0.004538484729619772 54-55 0.0062674312932844475 56-57 0.009806368790785579 58-59 0.01610081487412729 60-61 0.004700573469963335 62-63 0.0028905825361268786 64-65 0.010616812492503395 66-67 0.022314216587297212 68-69 0.02366495609016024 70-71 0.023746000460332022 72-73 0.021287654565121314 74-75 0.022719438438156124 76-77 0.022476305327640774 78-79 0.015020223271836866 80-81 0.0035119227074438715 82-83 0.0011616359724622037 84-85 0.005673105912024716 86-87 0.013723513349088359 88-89 0.01615484445424181 90-91 0.02223317221712543 92-93 0.022422275747526255 94-95 0.023935103990732846 96-97 0.025799124504683822 98-99 0.03109402335590689 100-101 0.021963024316552823 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1850838.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.1537188363267 #Duplication Level Percentage of deduplicated Percentage of total 1 82.62064735784612 38.95870775587175 2 8.115375078739506 7.653402294284308 3 2.7705589863290427 3.91926478382454 4 1.4515821404198486 2.7378998446876337 5 1.0126560574258954 2.3875249504881886 6 0.6719960302985939 1.9012267123096547 7 0.5298410233139015 1.7488782248906733 8 0.36940251834148574 1.393496198984435 9 0.2893346312530852 1.2278883466548218 >10 1.9031630281281995 17.310054155013653 >50 0.16354796221564352 5.339240321208561 >100 0.09624458118687265 8.158221821001112 >500 0.004057344904502377 1.2610646742719434 >1k 0.0013659850939699476 1.338733330629492 >5k 0.0 0.0 >10k+ 2.2727450344074576E-4 4.664396585879289 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT 72806 3.933677609817823 TruSeq Adapter, Index 27 (97% over 39bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14242 0.7694892799910095 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 4181 0.2258976744588127 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4107 0.22189948553033817 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 3710 0.20044974222487325 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT 2191 0.11837881003091573 TruSeq Adapter, Index 27 (97% over 39bp) GTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1853 0.1001168119522076 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.003295804386985787 0.0 0.0 2 0.0035659522875583923 0.0 0.0 3 0.0038901297682455193 0.0 0.0 4 0.0041602776688181246 0.0 0.0 5 0.004862662210306899 0.0 0.0 6 0.005294898851223068 0.0 0.0 7 0.0064835496137425315 0.0 0.0 8 0.007456082055803911 0.0 0.0 9 0.008536673658094334 0.0 0.0 10-11 0.017397524796875792 0.0 0.0 12-13 0.03287699949968609 0.0 0.0 14-15 0.052057500440341076 0.0 0.0 16-17 0.08126048849223974 0.0 0.0 18-19 0.11813567692040039 0.0 0.0 20-21 0.13067053950696927 0.0 0.0 22-23 0.13985556812643787 0.0 0.0 24-25 0.14801403472373054 0.0 0.0 26-27 0.1573611520835427 0.0 0.0 28-29 0.16897751180816473 0.0 0.0 30-31 0.22884228657505412 0.0 0.0 32-33 0.2421335632832263 0.0 0.0 34-35 0.2505081482009771 0.0 0.0 36-37 0.25866661479826975 0.0 0.0 38-39 0.2678246286276811 0.0 0.0 40-41 0.27706368682726423 0.0 0.0 42-43 0.2853302125847859 0.0 0.0 44-45 0.29408300456333836 0.0 0.0 46-47 0.30302490007229155 0.0 0.0 48-49 0.313020372393478 0.0 0.0 50-51 0.3250149391789017 0.0 0.0 52-53 0.33749577218535604 0.0 0.0 54-55 0.3513273446946734 0.0 0.0 56-57 0.3662935383863958 0.0 0.0 58-59 0.3828806194815538 0.0 0.0 60-61 0.4001700851182005 0.0 0.0 62-63 0.41997192623017254 0.0 0.0 64-65 0.44120555121517924 0.0 0.0 66-67 0.4650596108357403 0.0 0.0 68-69 0.49164216425208473 0.0 0.0 70-71 0.5188190430496888 0.0 0.0 72-73 0.5491566522839925 0.0 0.0 74-75 0.5861669146624394 0.0 0.0 76-77 0.6315787767486944 0.0 0.0 78-79 0.6835012032387491 0.0 0.0 80-81 0.7373957094029839 0.0 0.0 82-83 0.7966661587886136 0.0 0.0 84-85 0.8665534206667467 0.0 0.0 86-87 0.9407900637440987 0.0 0.0 88-89 1.023779498800003 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAAGA 11420 0.0 69.959465 4 ATCGGAA 11445 0.0 69.14261 2 GATCGGA 11575 0.0 68.64334 1 TCGGAAG 11725 0.0 67.612976 3 GAAGAGC 11815 0.0 67.57124 6 GGAAGAG 13895 0.0 57.72966 5 GAGCACA 15150 0.0 52.696648 9 AAGAGCA 15520 0.0 51.348545 7 AGAGCAC 15845 0.0 50.41522 8 TATGCCG 9185 0.0 43.334503 48-49 GTATGCC 9280 0.0 43.06944 46-47 GATCTCG 8920 0.0 42.808582 40-41 TACTCGA 9155 0.0 42.77206 36-37 CTCGTAT 9050 0.0 42.641457 44-45 GCCGTCT 9425 0.0 42.46067 50-51 TCGTATG 9475 0.0 42.43312 44-45 ACTCGAT 9265 0.0 42.28988 36-37 TCGATCT 9010 0.0 42.274403 38-39 TCTCGTA 9165 0.0 42.183567 42-43 CTCGATC 8970 0.0 42.14524 38-39 >>END_MODULE