FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n01_40825-1.341000000356f9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n01_40825-1.341000000356f9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1850838
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT728063.933677609817823TruSeq Adapter, Index 27 (97% over 39bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142420.7694892799910095No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA41810.2258976744588127No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41070.22189948553033817No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC37100.20044974222487325No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT21910.11837881003091573TruSeq Adapter, Index 27 (97% over 39bp)
GTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18530.1001168119522076No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA114200.069.9594654
ATCGGAA114450.069.142612
GATCGGA115750.068.643341
TCGGAAG117250.067.6129763
GAAGAGC118150.067.571246
GGAAGAG138950.057.729665
GAGCACA151500.052.6966489
AAGAGCA155200.051.3485457
AGAGCAC158450.050.415228
TATGCCG91850.043.33450348-49
GTATGCC92800.043.0694446-47
GATCTCG89200.042.80858240-41
TACTCGA91550.042.7720636-37
CTCGTAT90500.042.64145744-45
GCCGTCT94250.042.4606750-51
TCGTATG94750.042.4331244-45
ACTCGAT92650.042.2898836-37
TCGATCT90100.042.27440338-39
TCTCGTA91650.042.18356742-43
CTCGATC89700.042.1452438-39