##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n01_40255-1.341000000357f8.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7740804 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.668279160665996 33.0 33.0 33.0 33.0 33.0 2 32.7169353467676 33.0 33.0 33.0 33.0 33.0 3 32.560780508071254 33.0 33.0 33.0 33.0 33.0 4 36.607225941904744 37.0 37.0 37.0 37.0 37.0 5 36.669316649795036 37.0 37.0 37.0 37.0 37.0 6 36.55327069384524 37.0 37.0 37.0 37.0 37.0 7 36.475768279367365 37.0 37.0 37.0 37.0 37.0 8 36.419289520830134 37.0 37.0 37.0 37.0 37.0 9 36.44660993870921 37.0 37.0 37.0 37.0 37.0 10-11 36.39600763693281 37.0 37.0 37.0 37.0 37.0 12-13 36.378145022144984 37.0 37.0 37.0 37.0 37.0 14-15 38.22563567040323 40.0 37.0 40.0 37.0 40.0 16-17 38.21384522848014 40.0 37.0 40.0 37.0 40.0 18-19 38.20429150512014 40.0 37.0 40.0 37.0 40.0 20-21 38.14159175196788 40.0 37.0 40.0 37.0 40.0 22-23 37.99855389698538 40.0 37.0 40.0 33.0 40.0 24-25 37.98317241981582 40.0 37.0 40.0 35.0 40.0 26-27 37.905812044847025 40.0 37.0 40.0 33.0 40.0 28-29 37.7389295091311 40.0 37.0 40.0 33.0 40.0 30-31 37.697745415075744 40.0 37.0 40.0 33.0 40.0 32-33 37.567420774379514 40.0 37.0 40.0 33.0 40.0 34-35 37.43730496212021 40.0 37.0 40.0 33.0 40.0 36-37 37.28879532410328 40.0 37.0 40.0 33.0 40.0 38-39 37.23448675357237 40.0 37.0 40.0 33.0 40.0 40-41 37.19527738979052 40.0 37.0 40.0 33.0 40.0 42-43 36.97639999669285 38.5 37.0 40.0 33.0 40.0 44-45 36.86031644774884 37.0 37.0 40.0 33.0 40.0 46-47 36.8265566470873 37.0 37.0 40.0 33.0 40.0 48-49 36.60164338226365 37.0 37.0 40.0 33.0 40.0 50-51 36.44487090746646 37.0 37.0 40.0 33.0 40.0 52-53 36.31143625390851 37.0 37.0 40.0 33.0 40.0 54-55 36.12178172964979 37.0 37.0 40.0 33.0 40.0 56-57 35.93962410106237 37.0 37.0 40.0 33.0 40.0 58-59 35.72451543793126 37.0 37.0 40.0 27.0 40.0 60-61 35.40224968879202 37.0 33.0 40.0 27.0 40.0 62-63 35.158189446470935 37.0 33.0 38.5 27.0 40.0 64-65 34.87099595855935 37.0 33.0 37.0 27.0 40.0 66-67 34.44467583729029 37.0 33.0 37.0 27.0 40.0 68-69 34.18320138321549 37.0 33.0 37.0 27.0 40.0 70-71 33.84171166199273 37.0 33.0 37.0 27.0 40.0 72-73 33.3400779169709 37.0 33.0 37.0 27.0 37.0 74-75 32.8302265501103 37.0 33.0 37.0 22.0 37.0 76-77 30.2397102419852 33.0 30.0 35.0 22.0 37.0 78-79 32.51038367590757 35.0 33.0 37.0 24.5 37.0 80-81 32.881512695580454 37.0 33.0 37.0 27.0 37.0 82-83 32.7981603719717 37.0 33.0 37.0 27.0 37.0 84-85 32.63942595627017 37.0 33.0 37.0 22.0 37.0 86-87 32.48199999638281 37.0 33.0 37.0 22.0 37.0 88-89 32.36864826961127 33.0 33.0 37.0 22.0 37.0 90-91 32.244920618064995 33.0 33.0 37.0 22.0 37.0 92-93 32.07554396158332 33.0 33.0 37.0 22.0 37.0 94-95 31.956624789879708 33.0 33.0 37.0 22.0 37.0 96-97 31.815749371770686 33.0 33.0 37.0 22.0 37.0 98-99 31.637494955304383 33.0 33.0 37.0 18.5 37.0 100-101 29.986866157572262 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 0.14127435160926893 2306 2 0.12568869212040568 2306 3 0.33064281775321547 2306 4 0.2376795142853183 2306 5 0.1723227547804953 2306 6 0.15387343996569314 2306 7 0.19044798898094228 2306 8 0.17843048852665788 2306 9 0.16796411818902612 2306 10-11 0.1774421405019453 2306 12-13 0.15444885109914708 2306 14-15 0.33495282133696946 2306 16-17 0.34032814179559523 2306 18-19 0.3525782523598622 2306 20-21 0.37472381649010345 2306 22-23 0.4289748226260528 2306 24-25 0.4400054216759628 2306 26-27 0.41195886569371254 2306 28-29 0.46977236081119145 2306 30-31 0.40747187061362666 2306 32-33 0.4812284719545872 2306 34-35 0.4512579558673764 2306 36-37 0.4332701515990678 2306 38-39 0.477143782957306 2306 40-41 0.49614446849213323 2306 42-43 0.5447586725776574 2306 44-45 0.4319758991794842 2306 46-47 0.4663730705713576 2306 48-49 0.5111165117568177 2306 50-51 0.49509154081763285 2306 52-53 0.5031892565107725 2306 54-55 0.4761572868732813 2306 56-57 0.5659730369239853 2306 58-59 0.5865301997808743 2306 60-61 0.5512008397572856 2306 62-63 0.49879033178668664 2306 64-65 0.39207823826556876 2306 66-67 0.34840544871608614 2306 68-69 0.46569500594544877 2306 70-71 0.44716230735205187 2306 72-73 0.4855233473332561 2306 74-75 0.3628152613600477 2306 76-77 0.22693749036175248 2306 78-79 0.39561290849018604 2306 80-81 0.5114749279036843 2306 82-83 0.5091563986605081 2306 84-85 0.5765100445193596 2306 86-87 0.6114933252453341 2306 88-89 0.6716435798640887 2306 90-91 0.5931455196388242 2306 92-93 0.5897571127167538 2306 94-95 0.5624286888515755 2306 96-97 0.6343635518241086 2306 98-99 0.49728364759366883 2306 100-101 0.4721854699474477 2307 1 0.10187159173335658 2307 2 0.10805315947924043 2307 3 0.3000999429219462 2307 4 0.1885579180364232 2307 5 0.1077132410234043 2307 6 0.16688727994160502 2307 7 0.17087790443902406 2307 8 0.20853214857950064 2307 9 0.13504310855753943 2307 10-11 0.16907705743164314 2307 12-13 0.17635410595971024 2307 14-15 0.3606198702860297 2307 16-17 0.306438908965319 2307 18-19 0.37621237089446424 2307 20-21 0.40247437215278836 2307 22-23 0.329047659262919 2307 24-25 0.34594169215598214 2307 26-27 0.4554266744713189 2307 28-29 0.4245887468555125 2307 30-31 0.40831184314343005 2307 32-33 0.44210515036036924 2307 34-35 0.42823660556476284 2307 36-37 0.4195031138139953 2307 38-39 0.45562480246724135 2307 40-41 0.5218613494737525 2307 42-43 0.543597413684509 2307 44-45 0.45444473301520105 2307 46-47 0.441114353348695 2307 48-49 0.34052678195942576 2307 50-51 0.4008825101572384 2307 52-53 0.46952814139640253 2307 54-55 0.46872832204665116 2307 56-57 0.4458352122799667 2307 58-59 0.34754744308501273 2307 60-61 0.36753610480077015 2307 62-63 0.39756147995326074 2307 64-65 0.22937157741771586 2307 66-67 0.2184835290495144 2307 68-69 0.35707078277372517 2307 70-71 0.26554258022726174 2307 72-73 0.072911309835753 2307 74-75 0.09431012084589696 2307 76-77 -0.23177604480315495 2307 78-79 0.237548170827246 2307 80-81 0.41010043662683415 2307 82-83 0.5059049008239569 2307 84-85 0.4448003287953739 2307 86-87 0.4598709593571044 2307 88-89 0.4771228368153757 2307 90-91 0.4217492416484703 2307 92-93 0.313901223030598 2307 94-95 0.29628635801153536 2307 96-97 0.3551948976272996 2307 98-99 0.2189886270755892 2307 100-101 0.21800252244868545 2201 1 0.1366910587199257 2201 2 0.10797177260933921 2201 3 0.3049986797789046 2201 4 0.224918560663383 2201 5 0.11466687873422643 2201 6 0.13414435424828497 2201 7 0.15005086329866657 2201 8 0.17640691629760852 2201 9 0.14467122231098273 2201 10-11 0.1770383869073342 2201 12-13 0.18038722524087802 2201 14-15 0.34347687873051314 2201 16-17 0.3270707157067889 2201 18-19 0.36303451941374476 2201 20-21 0.34268277899305133 2201 22-23 0.2526809008596089 2201 24-25 0.26703241734592353 2201 26-27 0.2714634161738587 2201 28-29 0.4011643825434206 2201 30-31 0.31897940365433186 2201 32-33 0.3814255935189337 2201 34-35 0.3309534215974992 2201 36-37 0.29002248435947564 2201 38-39 0.26369551025074145 2201 40-41 0.3211373356426961 2201 42-43 0.38373243120856415 2201 44-45 0.40517203995660367 2201 46-47 0.38054464525939835 2201 48-49 0.35943229321480885 2201 50-51 0.41219034540763033 2201 52-53 0.409726198461307 2201 54-55 0.45510778244699424 2201 56-57 0.4712450869065492 2201 58-59 0.4656743689665035 2201 60-61 0.3600527467691137 2201 62-63 0.3677574200186484 2201 64-65 0.3247365246103371 2201 66-67 0.24038882860494937 2201 68-69 0.32694376280336 2201 70-71 0.32712656630210546 2201 72-73 0.3271860022827653 2201 74-75 0.2444768933625383 2201 76-77 0.04925822706662686 2201 78-79 0.4692979885300659 2201 80-81 0.46726271640007866 2201 82-83 0.5529451002923622 2201 84-85 0.5673103845430276 2201 86-87 0.5698152117034496 2201 88-89 0.5474441502959522 2201 90-91 0.5187264015760249 2201 92-93 0.4546539339660782 2201 94-95 0.5448293919070508 2201 96-97 0.5301566740251076 2201 98-99 0.5328999012740709 2201 100-101 0.23716842464330767 1101 1 0.1534174833120474 1101 2 0.11835226803351162 1101 3 0.32323699273003115 1101 4 0.23064969823905557 1101 5 0.14006252700995248 1101 6 0.14691499542356468 1101 7 0.12119373683422907 1101 8 0.16634043899519924 1101 9 0.19005467637330753 1101 10-11 0.15075523281922898 1101 12-13 0.14481985606533954 1101 14-15 0.2892916545662132 1101 16-17 0.25737514858309396 1101 18-19 0.2954508747312019 1101 20-21 0.3099968208067594 1101 22-23 0.30411690364669397 1101 24-25 0.255643558666641 1101 26-27 0.24863700188630133 1101 28-29 0.24572809545303897 1101 30-31 0.28739220917689323 1101 32-33 0.3187397580740239 1101 34-35 0.38866477725892423 1101 36-37 0.3649264249572255 1101 38-39 0.3642465334143239 1101 40-41 0.38547919493483107 1101 42-43 0.35835422362663394 1101 44-45 0.2667255576246532 1101 46-47 0.4508794808901371 1101 48-49 0.3635397621419685 1101 50-51 0.3584293354344581 1101 52-53 0.3030779686917242 1101 54-55 0.38419787490550306 1101 56-57 0.4044428195592715 1101 58-59 0.4355677067071113 1101 60-61 0.38806485591987894 1101 62-63 0.4014097953069822 1101 64-65 0.2454530573889926 1101 66-67 0.23535209325163464 1101 68-69 0.3724535548537702 1101 70-71 0.19839816129208998 1101 72-73 0.3019652604738283 1101 74-75 0.31855812417884977 1101 76-77 0.026647866152735844 1101 78-79 0.3171078255951656 1101 80-81 0.3782263618304924 1101 82-83 0.40529669668673307 1101 84-85 0.4727981659240683 1101 86-87 0.5661160434047474 1101 88-89 0.5606077779716827 1101 90-91 0.4686429802542449 1101 92-93 0.5557279924041083 1101 94-95 0.47254893413307997 1101 96-97 0.5474190502810998 1101 98-99 0.4772925754976143 1101 100-101 0.33173232309223266 2304 1 0.11004860711685183 2304 2 0.07450204997671506 2304 3 0.26908948057545246 2304 4 0.14074928723183433 2304 5 0.07184313235977413 2304 6 0.13638374619920768 2304 7 0.1551946534521278 2304 8 0.18706040589317752 2304 9 0.05644740291729988 2304 10-11 0.08928874529514985 2304 12-13 0.11807645116383725 2304 14-15 0.21703195038232082 2304 16-17 0.2575619909386049 2304 18-19 0.26466728797286976 2304 20-21 0.1826587888959068 2304 22-23 0.29029907261959664 2304 24-25 0.29964885306961975 2304 26-27 0.2958165224320197 2304 28-29 0.256068569873797 2304 30-31 0.31617395003387827 2304 32-33 0.2644823671910288 2304 34-35 0.2839659256665499 2304 36-37 0.23213250433813215 2304 38-39 0.2694150112372 2304 40-41 0.3284192963960777 2304 42-43 0.36880123164137046 2304 44-45 0.40320024966545276 2304 46-47 0.4668265027450502 2304 48-49 0.31697137978054 2304 50-51 0.3295649110258765 2304 52-53 0.26667121096863156 2304 54-55 0.320820089927885 2304 56-57 0.3950772603074384 2304 58-59 0.3126388939254454 2304 60-61 0.4134045300619107 2304 62-63 0.3166021088724307 2304 64-65 0.1955356081566464 2304 66-67 0.23050364009797164 2304 68-69 0.3345919445458989 2304 70-71 0.3260242577939536 2304 72-73 0.2640688454467295 2304 74-75 0.24083566227022146 2304 76-77 0.04965439840866637 2304 78-79 0.34898733967532536 2304 80-81 0.35111325573681285 2304 82-83 0.3648740412505873 2304 84-85 0.3733191029416716 2304 86-87 0.47572361674822616 2304 88-89 0.5764220924708994 2304 90-91 0.41205952078466623 2304 92-93 0.5793991594597045 2304 94-95 0.5971540678266081 2304 96-97 0.4773870524105419 2304 98-99 0.47926178718680745 2304 100-101 0.2870350710358949 2202 1 0.11172458139217412 2202 2 0.0759814910968899 2202 3 0.2902793830042967 2202 4 0.1768509160381555 2202 5 0.1306512948352463 2202 6 0.09369160480418515 2202 7 0.11456278424040534 2202 8 0.14312889618595648 2202 9 0.0824810917236789 2202 10-11 0.12551207347830484 2202 12-13 0.13074981608357916 2202 14-15 0.0855335816075069 2202 16-17 0.037709118682137444 2202 18-19 0.050830446774682514 2202 20-21 0.1241682542350162 2202 22-23 0.20844104817087583 2202 24-25 0.20823296774822353 2202 26-27 0.21840906687118888 2202 28-29 0.26837683666301615 2202 30-31 0.24494957871320366 2202 32-33 0.2732120675837919 2202 34-35 0.259311294891873 2202 36-37 0.20189194775623065 2202 38-39 0.2676596678030734 2202 40-41 0.15109090763535704 2202 42-43 0.1635025465993536 2202 44-45 0.1807640246742963 2202 46-47 0.29044389199862053 2202 48-49 0.2919359282229408 2202 50-51 0.26238322670649694 2202 52-53 0.17525865159293375 2202 54-55 0.14678408505386642 2202 56-57 0.21077980586533585 2202 58-59 0.22224679516300228 2202 60-61 0.23882488424489168 2202 62-63 0.15976683691589955 2202 64-65 0.16856884040138453 2202 66-67 0.10098950029335896 2202 68-69 0.024946702008868726 2202 70-71 0.12441238356308304 2202 72-73 0.1067791283637689 2202 74-75 0.028138108654466976 2202 76-77 -0.05297022628859693 2202 78-79 0.1995087572538523 2202 80-81 0.2087024591123523 2202 82-83 0.22374569905311148 2202 84-85 0.23038598166257884 2202 86-87 0.3506532427607496 2202 88-89 0.3288321936703582 2202 90-91 0.3403840302832606 2202 92-93 0.2809067199390043 2202 94-95 0.2974370755700235 2202 96-97 0.37914345410013084 2202 98-99 0.2924408470326654 2202 100-101 0.19632640271431256 1102 1 0.133516028463184 1102 2 0.10202573663967485 1102 3 0.29670860529910925 1102 4 0.20229826764014547 1102 5 0.11392097554233516 1102 6 0.07126160025520534 1102 7 0.0937576576413548 1102 8 0.12989803059053884 1102 9 0.11039679533438118 1102 10-11 0.10730836712405534 1102 12-13 0.11785395933812026 1102 14-15 0.13950004954571682 1102 16-17 0.15321716583844847 1102 18-19 0.14585222093280237 1102 20-21 0.12244786191754997 1102 22-23 0.18436179108558548 1102 24-25 0.14646323106461523 1102 26-27 0.18178370001241007 1102 28-29 0.21193408080426224 1102 30-31 0.21238683186737006 1102 32-33 0.21066121043445918 1102 34-35 0.23183192471985592 1102 36-37 0.17736331966282393 1102 38-39 0.20816697096766035 1102 40-41 0.20299758131207568 1102 42-43 0.1365364598683385 1102 44-45 0.21578619562800583 1102 46-47 0.2874846471573136 1102 48-49 0.2630014519293553 1102 50-51 0.19453571525256308 1102 52-53 0.14989831159579836 1102 54-55 0.28809532443072783 1102 56-57 0.21847976604406227 1102 58-59 0.23733338871535636 1102 60-61 0.1557691897321476 1102 62-63 0.20221560422788087 1102 64-65 0.08831523133290631 1102 66-67 0.0815041441763995 1102 68-69 0.029843443041635 1102 70-71 0.024624952630311725 1102 72-73 -0.05759642165870105 1102 74-75 -0.027125455731990655 1102 76-77 -0.03442697627761504 1102 78-79 0.09856820998770388 1102 80-81 0.10149707629671667 1102 82-83 0.13825651385868554 1102 84-85 0.0617835665945492 1102 86-87 0.20122129816474654 1102 88-89 0.26126980819194756 1102 90-91 0.21812450612620182 1102 92-93 0.21508371628140566 1102 94-95 0.08836165181357813 1102 96-97 0.018420690928991235 1102 98-99 -0.10512481200938595 1102 100-101 -0.066296869630591 2305 1 0.06969165669627841 2305 2 0.02733481808863303 2305 3 0.2398090878411736 2305 4 0.12377885325735605 2305 5 0.0163262718213133 2305 6 0.04832670255749605 2305 7 0.03742610930655843 2305 8 0.04184990811574352 2305 9 0.040199982117776756 2305 10-11 0.016371578843809687 2305 12-13 -0.014260590369275405 2305 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1213 50-51 0.006642031607363208 1213 52-53 0.034684769646951 1213 54-55 0.04058501047791907 1213 56-57 0.0784264675231583 1213 58-59 -0.3165438506195457 1213 60-61 -0.19891576645772346 1213 62-63 -0.013953490567239157 1213 64-65 0.1068694167940123 1213 66-67 0.17401232756174778 1213 68-69 0.12156809274064528 1213 70-71 0.18768723115643837 1213 72-73 0.2699233595549799 1213 74-75 0.23023226599456592 1213 76-77 0.3589885671216777 1213 78-79 -0.06624316948343534 1213 80-81 -0.22150584569768483 1213 82-83 -0.18142416103548697 1213 84-85 -0.226955500363772 1213 86-87 -0.20764493423613573 1213 88-89 -0.1679577736795963 1213 90-91 -0.06280725357100181 1213 92-93 -0.0890053701522433 1213 94-95 0.09797354494733668 1213 96-97 0.08049184198501536 1213 98-99 0.006213209769786232 1213 100-101 0.3070989518766112 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 5.0 9 38.0 10 41.0 11 44.0 12 92.0 13 296.0 14 1000.0 15 2894.0 16 6405.0 17 10865.0 18 13362.0 19 15485.0 20 18792.0 21 22688.0 22 28448.0 23 35452.0 24 43592.0 25 54448.0 26 69999.0 27 93231.0 28 135127.0 29 188639.0 30 181617.0 31 182495.0 32 241080.0 33 344041.0 34 530471.0 35 915835.0 36 1702918.0 37 2344725.0 38 556358.0 39 319.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.2609067714176 27.32786122191969 20.340418109133644 15.070813897529062 2 31.534049951400394 25.711476482287885 28.316658062909227 14.43781550340249 3 34.98793018497576 20.15957527893119 26.949340365166485 17.903154170926562 4 30.90279908924257 19.668388367413947 28.279997565593618 21.14881497774986 5 31.853112415712893 18.244513102256562 31.179060986429835 18.723313495600717 6 29.148638823564067 18.793474166249396 33.248574695858466 18.80931231432807 7 31.809615641992746 22.042839477656326 25.865569002909776 20.281975877441155 8 29.847093402700803 28.374223142712307 22.18166226660693 19.59702118797996 9 29.482686811344145 25.52619857058776 24.718530529903614 20.272584088164486 10-11 24.51185240170453 28.7190500095985 28.91306111491922 17.856036473777753 12-13 22.67702364690377 27.959932317090658 28.57760733319364 20.78543670281193 14-15 22.71514044277571 28.52092625003811 28.47304362699275 20.290889680193427 16-17 23.673710121067526 27.524750658975478 28.908670210484594 19.8928690094724 18-19 21.37402652230957 27.32105734753134 30.800211450903547 20.50470467925554 20-21 22.70809337117953 27.775228516314325 30.462262059600008 19.05441605290613 22-23 21.295979074008333 30.63438888260186 28.743745481735488 19.32588656165432 24-25 21.34975255800302 26.920995545165592 30.662402768497948 21.06684912833344 26-27 21.10159257399944 27.13483047363826 29.141857652475807 22.621719299886497 28-29 22.360729403211092 29.34095003690836 28.51506576715515 19.783254792725398 30-31 20.16878350104201 28.745076092870974 29.987576225932088 21.09856418015493 32-33 20.058620525723168 30.18778798688095 28.68370003942743 21.069891447968452 34-35 22.0839592373092 29.924249470726817 28.80306102570224 19.188730266261746 36-37 21.717349251220107 30.523674394365592 29.034001461878383 18.72497489253592 38-39 20.355319093001263 28.621645121303 31.41534349241266 19.60769229328307 40-41 20.56928770148894 28.04951724170896 28.119232451878062 23.26196260492404 42-43 20.82995565165863 29.674361558414848 29.81312694885504 19.68255584107148 44-45 20.260492021825467 28.13317969668281 30.021472657548674 21.584855623943056 46-47 21.996766254846793 28.044002756647917 28.630362103622858 21.328868884882432 48-49 20.829822052167764 29.710341427657315 29.463316903358134 19.996519616816787 50-51 22.09041442458442 28.302794419906412 28.900632060326497 20.70615909518267 52-53 20.523990359802106 28.161295677736785 27.78431123033962 23.530402732121484 54-55 22.41051549552323 27.96636353001418 29.492715993343193 20.13040498111939 56-57 20.929571547174824 27.587084994706775 29.47351849760765 22.009824960510755 58-59 20.66418559230423 27.802977793141913 29.797564410780588 21.735272203773267 60-61 21.83132586155276 27.93510590297796 29.581365184163854 20.652203051305428 62-63 20.156792825979238 28.103719138441075 29.621354116572284 22.118133919007406 64-65 22.02570174569339 28.209071579798444 29.574468744844168 20.190757929663995 66-67 21.08767151378568 30.799568862618994 27.877620092195897 20.235139531399433 68-69 20.925480521512753 30.553284025352667 28.05016649955656 20.47106895357802 70-71 20.669651634126577 31.247603419069364 27.917073890252603 20.16567105655146 72-73 20.774345736928726 31.43877209923031 27.714495446321013 20.072386717519947 74-75 21.576630730855626 30.727974111330376 27.41192513125754 20.283470026556458 76-77 21.631963443237375 29.721293320559038 27.709348204029773 20.937395032173818 78-79 21.279653782010968 29.660122751306346 28.271290658826505 20.788932807856185 80-81 20.844057140007592 30.170003072126768 28.10530263549203 20.880637152373613 82-83 20.151839831488598 30.124739648010895 28.32857010440563 21.39485041609488 84-85 20.399901684699213 29.890777643669047 28.45207339024037 21.257247281391376 86-87 20.23333408271464 30.103067062650084 28.888668811964468 20.774930042670807 88-89 20.265872548782728 30.457868928944787 28.787253508688398 20.489005013584084 90-91 20.782361209422263 29.591748789319183 28.547316112062564 21.07857388919599 92-93 20.42009641256214 30.03288007687782 28.02600790047025 21.52101561008979 94-95 20.23330674259639 30.390214488202183 27.67748121650974 21.698997552691687 96-97 20.494182862997572 30.754803496736677 27.388907255897237 21.362106384368513 98-99 20.66948716238953 29.991854859094104 27.71198430260898 21.62667367590739 100-101 20.90903199884275 30.1471415305673 27.278615948181205 21.66521052240874 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3493.0 1 2166.0 2 1038.0 3 1674.0 4 1717.5 5 1227.0 6 1208.0 7 1369.0 8 1427.0 9 1385.5 10 1323.0 11 1267.0 12 1286.0 13 1323.0 14 1381.0 15 1442.5 16 1537.5 17 1734.0 18 2033.5 19 2365.5 20 2807.0 21 3667.0 22 4883.0 23 6684.0 24 9028.0 25 11925.0 26 16126.5 27 21479.0 28 27649.0 29 34932.5 30 43537.5 31 53608.5 32 68977.5 33 91986.5 34 124086.0 35 176209.0 36 247582.5 37 334407.5 38 419319.0 39 460722.5 40 487634.5 41 484499.5 42 457354.5 43 467052.5 44 463233.5 45 448374.5 46 459447.5 47 427422.0 48 339799.0 49 267462.5 50 222676.5 51 196356.0 52 176587.0 53 151495.0 54 119378.5 55 87497.0 56 65642.0 57 53965.0 58 46307.0 59 36145.0 60 26010.0 61 19189.5 62 13919.5 63 9684.0 64 6781.0 65 4747.0 66 3530.5 67 2654.0 68 1877.0 69 1235.5 70 787.0 71 510.0 72 351.0 73 257.5 74 192.0 75 132.0 76 92.5 77 63.5 78 44.5 79 34.5 80 27.0 81 25.0 82 17.5 83 14.0 84 10.5 85 5.5 86 3.5 87 3.0 88 3.0 89 2.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16810915248596917 2 0.0 3 7.751132828062821E-5 4 0.02262038930323 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.2918554713438034E-4 12-13 2.583710942687607E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 2.583710942687607E-5 28-29 1.2918554713438034E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0010076472676481668 38-39 5.619571300345545E-4 40-41 3.294231451926699E-4 42-43 5.296607432509594E-4 44-45 3.81097364046422E-4 46-47 0.003145668072722162 48-49 0.003578439655622336 50-51 0.004192071004510642 52-53 0.003862647859317973 54-55 0.005884401671971025 56-57 0.009327196503102262 58-59 0.016542209310557405 60-61 0.004476279208206279 62-63 0.0026806001030383925 64-65 0.010141065450048857 66-67 0.022471825924025465 68-69 0.02447420190460836 70-71 0.02473257299887712 72-73 0.021664416254435586 74-75 0.023511769578457225 76-77 0.023582821629381134 78-79 0.015134086846792658 80-81 0.0030746160217982525 82-83 0.0010528622091452 84-85 0.005186799717445371 86-87 0.014029550418793706 88-89 0.015902740852242223 90-91 0.022865841842785322 92-93 0.02287876039749876 94-95 0.024461283349894922 96-97 0.025688546047671533 98-99 0.031624621938496314 100-101 0.022717278463580785 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 7740804.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.319771859540154 #Duplication Level Percentage of deduplicated Percentage of total 1 88.00690277776614 36.364251448420276 2 6.135852359889468 5.070640393489078 3 1.743006497533208 2.1606189248330487 4 0.8134586428448881 1.3444770215808775 5 0.6355306244041397 1.3129990205065076 6 0.2997130441060618 0.7430444763474461 7 0.3017559753221619 0.872794164029533 8 0.215270188641194 0.7115932066251446 9 0.1392482228961311 0.517833432172308 >10 1.3188176697642464 11.015258133526661 >50 0.19214903758515997 5.6752803225738075 >100 0.1618708711351963 13.90159739722483 >500 0.021602448466686595 6.395568690791191 >1k 0.01444618791532566 10.007719823326 >5k 2.5038010709654444E-4 0.6925581211301516 >10k+ 1.2507162282828112E-4 3.2137654234232946 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT 209053 2.7006626185083618 TruSeq Adapter, Index 7 (97% over 38bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17123 0.22120441235819946 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 12231 0.1580068427000606 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 10340 0.1335778557369493 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 9665 0.12485783130537861 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 9635 0.12447027466397546 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0027904078181026156 0.0 0.0 2 0.002919593365236996 0.0 0.0 3 0.003268394342499823 1.2918554713438036E-5 0.0 4 0.0034363355537745176 1.2918554713438036E-5 0.0 5 0.0039659962970254765 1.2918554713438036E-5 0.0 6 0.004689435360978007 1.2918554713438036E-5 0.0 7 0.005399955870217099 1.2918554713438036E-5 0.0 8 0.006084639270029315 1.2918554713438036E-5 0.0 9 0.006691811341560903 1.2918554713438036E-5 1.2918554713438036E-5 10-11 0.011426461644035942 1.2918554713438036E-5 1.2918554713438036E-5 12-13 0.015340783722207669 1.2918554713438036E-5 1.2918554713438036E-5 14-15 0.021095999847044314 1.2918554713438036E-5 1.2918554713438036E-5 16-17 0.02933157847686106 1.2918554713438036E-5 1.2918554713438036E-5 18-19 0.039685800079681643 1.2918554713438036E-5 2.5837109426876072E-5 20-21 0.04591254345155878 1.2918554713438036E-5 2.5837109426876072E-5 22-23 0.051609626080184955 1.2918554713438036E-5 2.5837109426876072E-5 24-25 0.05835957091795632 1.2918554713438036E-5 3.229638678359509E-5 26-27 0.06516118997458145 1.2918554713438036E-5 3.8755664140314105E-5 28-29 0.07323528667048022 1.2918554713438036E-5 3.8755664140314105E-5 30-31 0.1213891993648205 1.2918554713438036E-5 3.8755664140314105E-5 32-33 0.13223432604675173 1.2918554713438036E-5 3.8755664140314105E-5 34-35 0.14136774422915244 1.2918554713438036E-5 5.1674218853752144E-5 36-37 0.15084996338881596 1.2918554713438036E-5 6.459277356719018E-5 38-39 0.16147547464061873 1.2918554713438036E-5 6.459277356719018E-5 40-41 0.1731538481015667 1.2918554713438036E-5 6.459277356719018E-5 42-43 0.18560087556796426 1.2918554713438036E-5 6.459277356719018E-5 44-45 0.1994301883886997 1.2918554713438036E-5 6.459277356719018E-5 46-47 0.21309801927551714 1.2918554713438036E-5 6.459277356719018E-5 48-49 0.22851631432600542 1.2918554713438036E-5 6.459277356719018E-5 50-51 0.24531043545347486 1.2918554713438036E-5 6.459277356719018E-5 52-53 0.26252440960913104 1.2918554713438036E-5 6.459277356719018E-5 54-55 0.28189578240193136 1.2918554713438036E-5 6.459277356719018E-5 56-57 0.303489146605443 1.2918554713438036E-5 7.751132828062821E-5 58-59 0.3272011537819586 1.2918554713438036E-5 7.751132828062821E-5 60-61 0.35239233547316273 1.2918554713438036E-5 7.751132828062821E-5 62-63 0.3778871032001327 1.2918554713438036E-5 7.751132828062821E-5 64-65 0.4071863852902102 1.2918554713438036E-5 7.751132828062821E-5 66-67 0.4397734395548576 1.2918554713438036E-5 7.751132828062821E-5 68-69 0.475280087184742 1.2918554713438036E-5 7.751132828062821E-5 70-71 0.513351067925244 1.2918554713438036E-5 8.397060563734724E-5 72-73 0.5553299114665609 1.2918554713438036E-5 9.042988299406625E-5 74-75 0.6018625455443647 1.2918554713438036E-5 9.042988299406625E-5 76-77 0.6540922622507946 1.2918554713438036E-5 9.042988299406625E-5 78-79 0.7127102042630197 1.2918554713438036E-5 9.042988299406625E-5 80-81 0.7793441094749332 1.2918554713438036E-5 9.042988299406625E-5 82-83 0.8524566698756357 1.2918554713438036E-5 9.042988299406625E-5 84-85 0.9350385308812883 1.2918554713438036E-5 1.0334843770750429E-4 86-87 1.0269669662221133 1.2918554713438036E-5 1.098077150642233E-4 88-89 1.12442454298029 1.2918554713438036E-5 1.2918554713438037E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAAGA 35120 0.0 64.89402 4 TCGGAAG 35055 0.0 64.67559 3 GATCGGA 35860 0.0 63.484394 1 GAAGAGC 36120 0.0 63.099945 6 ATCGGAA 36425 0.0 62.308243 2 GGAAGAG 41015 0.0 55.719715 5 GCCGTCT 26280 0.0 43.322502 50-51 TATGCCG 26340 0.0 43.068874 48-49 TGCCGTC 26395 0.0 42.98979 50-51 ATGCCGT 26420 0.0 42.94745 48-49 TCGTATG 26740 0.0 42.770145 44-45 CGTATGC 26610 0.0 42.755985 46-47 TCTCGTA 26395 0.0 42.51101 42-43 CTCGTAT 26420 0.0 42.47922 44-45 CATCTCG 26200 0.0 42.40085 40-41 CGTCTTC 26985 0.0 42.219532 52-53 ATCTCGT 26375 0.0 42.129063 42-43 GTATGCC 27400 0.0 41.592575 46-47 CGAGATT 27505 0.0 40.6557 32-33 GTCACGA 27625 0.0 40.57287 28-29 >>END_MODULE