FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n01_40255-1.341000000357f8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n01_40255-1.341000000357f8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7740804
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT2090532.7006626185083618TruSeq Adapter, Index 7 (97% over 38bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171230.22120441235819946No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA122310.1580068427000606No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC103400.1335778557369493No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG96650.12485783130537861No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC96350.12447027466397546No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA351200.064.894024
TCGGAAG350550.064.675593
GATCGGA358600.063.4843941
GAAGAGC361200.063.0999456
ATCGGAA364250.062.3082432
GGAAGAG410150.055.7197155
GCCGTCT262800.043.32250250-51
TATGCCG263400.043.06887448-49
TGCCGTC263950.042.9897950-51
ATGCCGT264200.042.9474548-49
TCGTATG267400.042.77014544-45
CGTATGC266100.042.75598546-47
TCTCGTA263950.042.5110142-43
CTCGTAT264200.042.4792244-45
CATCTCG262000.042.4008540-41
CGTCTTC269850.042.21953252-53
ATCTCGT263750.042.12906342-43
GTATGCC274000.041.59257546-47
CGAGATT275050.040.655732-33
GTCACGA276250.040.5728728-29