##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n01_35011-1.341000000358a7.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6100707 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.68234091556929 33.0 33.0 33.0 33.0 33.0 2 32.732881451280974 33.0 33.0 33.0 33.0 33.0 3 32.57194961174172 33.0 33.0 33.0 33.0 33.0 4 36.621571073647694 37.0 37.0 37.0 37.0 37.0 5 36.688708046460846 37.0 37.0 37.0 37.0 37.0 6 36.57062173285818 37.0 37.0 37.0 37.0 37.0 7 36.49503491972324 37.0 37.0 37.0 37.0 37.0 8 36.44457699738735 37.0 37.0 37.0 37.0 37.0 9 36.47870812350109 37.0 37.0 37.0 37.0 37.0 10-11 36.42016236806652 37.0 37.0 37.0 37.0 37.0 12-13 36.40079428499025 37.0 37.0 37.0 37.0 37.0 14-15 38.25928322733742 40.0 37.0 40.0 37.0 40.0 16-17 38.27231294667979 40.0 37.0 40.0 37.0 40.0 18-19 38.2683831562473 40.0 37.0 40.0 37.0 40.0 20-21 38.23689795297496 40.0 37.0 40.0 37.0 40.0 22-23 38.09894918736468 40.0 37.0 40.0 37.0 40.0 24-25 38.08651497277283 40.0 37.0 40.0 37.0 40.0 26-27 38.03746787052714 40.0 37.0 40.0 37.0 40.0 28-29 37.879113846968885 40.0 37.0 40.0 33.0 40.0 30-31 37.86268411185786 40.0 37.0 40.0 33.0 40.0 32-33 37.74350046642135 40.0 37.0 40.0 33.0 40.0 34-35 37.606202936807165 40.0 37.0 40.0 33.0 40.0 36-37 37.39895441626683 40.0 37.0 40.0 33.0 40.0 38-39 37.45809780735249 40.0 37.0 40.0 33.0 40.0 40-41 37.320949194904784 40.0 37.0 40.0 33.0 40.0 42-43 37.154811729197945 40.0 37.0 40.0 33.0 40.0 44-45 37.11281692433353 40.0 37.0 40.0 33.0 40.0 46-47 37.0740779716187 40.0 37.0 40.0 33.0 40.0 48-49 36.856222401764256 37.0 37.0 40.0 33.0 40.0 50-51 36.73296521206477 37.0 37.0 40.0 33.0 40.0 52-53 36.62376196726052 37.0 37.0 40.0 33.0 40.0 54-55 36.43686067860659 37.0 37.0 40.0 33.0 40.0 56-57 36.28543339321164 37.0 37.0 40.0 33.0 40.0 58-59 36.045475876812304 37.0 37.0 40.0 33.0 40.0 60-61 35.77242555657894 37.0 37.0 40.0 27.0 40.0 62-63 35.544936840926795 37.0 35.0 40.0 27.0 40.0 64-65 35.26132880992318 37.0 33.0 38.5 27.0 40.0 66-67 34.82300469437395 37.0 33.0 37.0 27.0 40.0 68-69 34.58755927796565 37.0 33.0 37.0 27.0 40.0 70-71 34.22910787225153 37.0 33.0 37.0 27.0 40.0 72-73 33.67822081932471 37.0 33.0 37.0 27.0 40.0 74-75 33.12216010373879 37.0 33.0 37.0 22.0 37.0 76-77 30.566452380027428 33.0 30.0 35.0 22.0 37.0 78-79 32.740793403125245 35.0 33.0 37.0 24.5 37.0 80-81 33.0277215411263 37.0 33.0 37.0 27.0 37.0 82-83 32.93610412694791 37.0 33.0 37.0 27.0 37.0 84-85 32.77041316686738 37.0 33.0 37.0 22.0 37.0 86-87 32.60998397398859 37.0 33.0 37.0 22.0 37.0 88-89 32.481140710412745 37.0 33.0 37.0 22.0 37.0 90-91 32.34511032901597 35.0 33.0 37.0 22.0 37.0 92-93 32.18253392598595 33.0 33.0 37.0 22.0 37.0 94-95 32.06158966493555 33.0 33.0 37.0 22.0 37.0 96-97 31.921996335834518 33.0 33.0 37.0 22.0 37.0 98-99 31.760547015288555 33.0 33.0 37.0 22.0 37.0 100-101 30.157317668263694 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 0.13210694812392632 2306 2 0.12030560355714925 2306 3 0.3379977958546192 2306 4 0.24212948580883875 2306 5 0.1654585218368254 2306 6 0.1576394494504214 2306 7 0.22979270255152784 2306 8 0.1989914229227523 2306 9 0.18839587411117265 2306 10-11 0.22474530088579314 2306 12-13 0.24420529115088385 2306 14-15 0.43420967810624944 2306 16-17 0.426371977829902 2306 18-19 0.41233868718066446 2306 20-21 0.3984411214321213 2306 22-23 0.5068872972455551 2306 24-25 0.46948049720041496 2306 26-27 0.4587522084949214 2306 28-29 0.5072700826198826 2306 30-31 0.4762008295154203 2306 32-33 0.5210778219513017 2306 34-35 0.4887299127107525 2306 36-37 0.5391374675489402 2306 38-39 0.4968061550100842 2306 40-41 0.549046485428839 2306 42-43 0.5366908681592477 2306 44-45 0.5554567751729351 2306 46-47 0.5993271936019227 2306 48-49 0.602007517073595 2306 50-51 0.5645680600055414 2306 52-53 0.6452806082469706 2306 54-55 0.6799966031879094 2306 56-57 0.6219606695022932 2306 58-59 0.5964116196926952 2306 60-61 0.5785270599900301 2306 62-63 0.598070824809021 2306 64-65 0.5354961679148857 2306 66-67 0.3886821765590014 2306 68-69 0.4910980828344975 2306 70-71 0.5714281739558729 2306 72-73 0.408895423062944 2306 74-75 0.2523702841372 2306 76-77 0.11855602486412664 2306 78-79 0.2959291338844139 2306 80-81 0.3156797769582269 2306 82-83 0.3665426837582615 2306 84-85 0.4109731985346059 2306 86-87 0.359141289568079 2306 88-89 0.5131136169016699 2306 90-91 0.5244921108750731 2306 92-93 0.39122413517152665 2306 94-95 0.48042462635452665 2306 96-97 0.5668833020655057 2306 98-99 0.29681815184149585 2306 100-101 0.3649361209506381 2307 1 0.13286561910796735 2307 2 0.11342042085350101 2307 3 0.32117582331365213 2307 4 0.21553420959845937 2307 5 0.15812170971654638 2307 6 0.15594259589928328 2307 7 0.2110265823081221 2307 8 0.1667026354254162 2307 9 0.16039947385932862 2307 10-11 0.15175559494812063 2307 12-13 0.14516622801190948 2307 14-15 0.32410349469308386 2307 16-17 0.2963303575374354 2307 18-19 0.31531173386884603 2307 20-21 0.2859410911319671 2307 22-23 0.29727168007000415 2307 24-25 0.24342291631269575 2307 26-27 0.3479256669599664 2307 28-29 0.4136229881174245 2307 30-31 0.3014213450633463 2307 32-33 0.29761844566045426 2307 34-35 0.37545296400007544 2307 36-37 0.33125257276410025 2307 38-39 0.26193354090037246 2307 40-41 0.3368989199946455 2307 42-43 0.3328438393205886 2307 44-45 0.3689198431448446 2307 46-47 0.34890599900732866 2307 48-49 0.3726716390060858 2307 50-51 0.2757847822434343 2307 52-53 0.34734913022877123 2307 54-55 0.37059485572636675 2307 56-57 0.4154939117346501 2307 58-59 0.3811732589247825 2307 60-61 0.36974662730023056 2307 62-63 0.3345379677252893 2307 64-65 0.3237589339087208 2307 66-67 0.2007816702366796 2307 68-69 0.17909359697515015 2307 70-71 0.25875611957755495 2307 72-73 0.11966439879292068 2307 74-75 0.18776680812140967 2307 76-77 -0.18525173048515242 2307 78-79 0.23229411814584466 2307 80-81 0.3243307628983345 2307 82-83 0.39263311450256566 2307 84-85 0.4433501931489019 2307 86-87 0.41830337402839035 2307 88-89 0.4530478247169185 2307 90-91 0.26657923670295247 2307 92-93 0.2951461153705708 2307 94-95 0.39971212921508226 2307 96-97 0.5112642259140756 2307 98-99 0.23900357137114625 2307 100-101 0.11703821101490774 2201 1 0.11893602102689016 2201 2 0.08632909294072988 2201 3 0.2763490114394287 2201 4 0.23126896125910434 2201 5 0.16222514559959222 2201 6 0.15681936305652044 2201 7 0.16494105450831853 2201 8 0.15881570172296477 2201 9 0.13253453283623884 2201 10-11 0.1821187056416207 2201 12-13 0.19613708416346753 2201 14-15 0.2560564618546053 2201 16-17 0.27491474101163504 2201 18-19 0.22631572772995412 2201 20-21 0.22755632298864015 2201 22-23 0.29203891435710005 2201 24-25 0.1901387820809859 2201 26-27 0.25993163215320436 2201 28-29 0.30767051805533185 2201 30-31 0.3290189810868682 2201 32-33 0.3359888364444785 2201 34-35 0.27540131094168885 2201 36-37 0.3220733410446286 2201 38-39 0.3334166764387092 2201 40-41 0.3112942860546468 2201 42-43 0.26370959155183726 2201 44-45 0.3727257290252126 2201 46-47 0.397538316587287 2201 48-49 0.3885055740009591 2201 50-51 0.35095362624055326 2201 52-53 0.3596453291753292 2201 54-55 0.4585688680031339 2201 56-57 0.43950633212397605 2201 58-59 0.4963708576405068 2201 60-61 0.4119874491667872 2201 62-63 0.35739216501361426 2201 64-65 0.3019817290775606 2201 66-67 0.3031655256229868 2201 68-69 0.3346362979350346 2201 70-71 0.37157547259469936 2201 72-73 0.28668604999099045 2201 74-75 0.2607354971086622 2201 76-77 0.022684904712630072 2201 78-79 0.42778007677642194 2201 80-81 0.37598635578233797 2201 82-83 0.47664955122554176 2201 84-85 0.5072810331337436 2201 86-87 0.608388994421837 2201 88-89 0.5319853819648799 2201 90-91 0.4909806010736304 2201 92-93 0.44699402592241455 2201 94-95 0.5160016739230286 2201 96-97 0.5224204501414036 2201 98-99 0.4082826994634736 2201 100-101 0.2799798281898802 1101 1 0.14110127982443288 1101 2 0.10660178856371516 1101 3 0.29591850078821125 1101 4 0.19889875012879088 1101 5 0.15185382630961897 1101 6 0.1823496199941559 1101 7 0.10951312993372397 1101 8 0.14591797227441106 1101 9 0.1594142371320828 1101 10-11 0.16895577423358787 1101 12-13 0.17892608875357752 1101 14-15 0.2735031040625273 1101 16-17 0.24793806877345048 1101 18-19 0.21265834323780552 1101 20-21 0.25609492989498506 1101 22-23 0.2515387297962377 1101 24-25 0.25069401074656383 1101 26-27 0.2999090002088707 1101 28-29 0.32629575177548276 1101 30-31 0.4028156768515956 1101 32-33 0.33143611011590224 1101 34-35 0.4111957576539709 1101 36-37 0.2609452825025329 1101 38-39 0.3103691722370243 1101 40-41 0.3355884301443197 1101 42-43 0.3257575942187927 1101 44-45 0.3017094430189644 1101 46-47 0.3478241912699076 1101 48-49 0.3466234472941707 1101 50-51 0.25711340652812 1101 52-53 0.2912055064236725 1101 54-55 0.3281884581175376 1101 56-57 0.42095470823469583 1101 58-59 0.3080085043695604 1101 60-61 0.3821848426875647 1101 62-63 0.3770643029338885 1101 64-65 0.341819305986526 1101 66-67 0.1932662070283584 1101 68-69 0.15570037273056414 1101 70-71 0.11112919283475264 1101 72-73 0.03484441385538872 1101 74-75 0.05042788031453682 1101 76-77 -0.19791740246497014 1101 78-79 0.20147060436306674 1101 80-81 0.3800472111309645 1101 82-83 0.4205853564614799 1101 84-85 0.3405014954114307 1101 86-87 0.36085800241873045 1101 88-89 0.3183331855158542 1101 90-91 0.31823096046279886 1101 92-93 0.23802628283876004 1101 94-95 0.29684427250816015 1101 96-97 0.23508882518115115 1101 98-99 0.15452669130433705 1101 100-101 0.25813915974746493 2304 1 0.12459286586528151 2304 2 0.06581359265197051 2304 3 0.2803774267705279 2304 4 0.1631940009520747 2304 5 0.10009124149102178 2304 6 0.10327065082575615 2304 7 0.11827661967608805 2304 8 0.13098636082980875 2304 9 0.11707686432720266 2304 10-11 0.14146410753183858 2304 12-13 0.07552390483740368 2304 14-15 0.20644573059581006 2304 16-17 0.2281645501179952 2304 18-19 0.27733871295134804 2304 20-21 0.21886977435867294 2304 22-23 0.26340118271357227 2304 24-25 0.24532856477042486 2304 26-27 0.2717682791343563 2304 28-29 0.29349076953158715 2304 30-31 0.21961328453188145 2304 32-33 0.3144207955498217 2304 34-35 0.34913599944611207 2304 36-37 0.3478950238890377 2304 38-39 0.3320091007738313 2304 40-41 0.36183666688732785 2304 42-43 0.35972126445839336 2304 44-45 0.38036023352168513 2304 46-47 0.3999096597720069 2304 48-49 0.369468931358206 2304 50-51 0.3326436282562355 2304 52-53 0.3484862906544919 2304 54-55 0.41748833475509883 2304 56-57 0.3743799787497366 2304 58-59 0.2513550396475992 2304 60-61 0.3819258155541121 2304 62-63 0.3826676136356397 2304 64-65 0.2656435384873035 2304 66-67 0.20597587644643056 2304 68-69 0.25097960830154875 2304 70-71 0.28131816316506075 2304 72-73 0.17191320024927847 2304 74-75 0.10560562718214328 2304 76-77 -0.0995826734468288 2304 78-79 0.14401533183225013 2304 80-81 0.2032007430334204 2304 82-83 0.27171330172020447 2304 84-85 0.20256429317629454 2304 86-87 0.30979542056213205 2304 88-89 0.3917438794529957 2304 90-91 0.2802694417715941 2304 92-93 0.42589012944652893 2304 94-95 0.46171508911931625 2304 96-97 0.29999263742088544 2304 98-99 0.2559666251151249 2304 100-101 0.19293139180682317 2202 1 0.09966203608026802 2202 2 0.09207377162015717 2202 3 0.26543137343795564 2202 4 0.15899071575660173 2202 5 0.10215699420258773 2202 6 0.10744014733707985 2202 7 0.12662893160833733 2202 8 0.16663576103830025 2202 9 0.11680547036267086 2202 10-11 0.10898743765236674 2202 12-13 0.10869449668501119 2202 14-15 0.09902814855065145 2202 16-17 0.05577880128348056 2202 18-19 0.06408051792455183 2202 20-21 0.07311354158344585 2202 22-23 0.14745858348599938 2202 24-25 0.1515140798970478 2202 26-27 0.11733442090719848 2202 28-29 0.16073689349166642 2202 30-31 0.19159148489979572 2202 32-33 0.2300196110141215 2202 34-35 0.23072565705862758 2202 36-37 0.1864072610185019 2202 38-39 0.26885762647560085 2202 40-41 0.24820215335626727 2202 42-43 0.19071706137653877 2202 44-45 0.1832435571623776 2202 46-47 0.2802436966691175 2202 48-49 0.29563631853383043 2202 50-51 0.32213727923709 2202 52-53 0.1939029251253217 2202 54-55 0.2488515709044563 2202 56-57 0.20584739385100193 2202 58-59 0.3079828408549048 2202 60-61 0.28382057447846165 2202 62-63 0.2645370530756068 2202 64-65 0.2038545073041078 2202 66-67 0.1425934563226079 2202 68-69 0.11605024257988106 2202 70-71 0.1051455399295591 2202 72-73 0.1280166498807631 2202 74-75 0.0937932624893918 2202 76-77 -0.15348857077531974 2202 78-79 0.10037775461307774 2202 80-81 0.19269452957569655 2202 82-83 0.2764014562276813 2202 84-85 0.22736586514652402 2202 86-87 0.32983324558758653 2202 88-89 0.3416232128432668 2202 90-91 0.3042109612524513 2202 92-93 0.38716077375640623 2202 94-95 0.36843796971817255 2202 96-97 0.4787382333692207 2202 98-99 0.27634631909876717 2202 100-101 0.24754485197141918 1102 1 0.09526878884213374 1102 2 0.07377765318422291 1102 3 0.28073974834988036 1102 4 0.16831216354957945 1102 5 0.06454685101969915 1102 6 0.05922486340232069 1102 7 0.06042270612974221 1102 8 0.07347042341321242 1102 9 0.07614303825558721 1102 10-11 0.09303481124607771 1102 12-13 0.08292105688968121 1102 14-15 0.01410590203272477 1102 16-17 0.07053210525396736 1102 18-19 0.04045173392609058 1102 20-21 0.1181443582745132 1102 22-23 0.13447273797375914 1102 24-25 0.08446268667840684 1102 26-27 0.14136880742040603 1102 28-29 0.22558441554630093 1102 30-31 0.12271686287713379 1102 32-33 0.17404964900035935 1102 34-35 0.1197423114618914 1102 36-37 0.07467069075176624 1102 38-39 0.16844406141149193 1102 40-41 0.105068308426425 1102 42-43 0.0784966931102602 1102 44-45 0.1771651094760074 1102 46-47 0.18234814971673075 1102 48-49 0.1489845542928876 1102 50-51 0.12162273604692331 1102 52-53 -0.011889065785020136 1102 54-55 0.1713438878568141 1102 56-57 0.13845156349685084 1102 58-59 0.139702517516902 1102 60-61 0.044381417455682026 1102 62-63 0.18902211988301332 1102 64-65 0.08224356331626126 1102 66-67 -0.11288997767468345 1102 68-69 -0.04221013863448064 1102 70-71 -0.10225959848229849 1102 72-73 -0.06983469679758514 1102 74-75 -0.16531222146461033 1102 76-77 -0.18966019003936196 1102 78-79 0.03166326108321016 1102 80-81 -0.00930813284080756 1102 82-83 -0.05082961760496829 1102 84-85 -0.017506773063828973 1102 86-87 0.06619326851972573 1102 88-89 0.04128277323254537 1102 90-91 -0.009876868592101573 1102 92-93 0.05792294248185215 1102 94-95 -1.2013284206346952E-4 1102 96-97 0.032200284833940884 1102 98-99 -0.10844709662040586 1102 100-101 -0.2462160843463863 2305 1 0.09919704945456687 2305 2 0.05111120778828848 2305 3 0.24139144627004328 2305 4 0.1163160343969949 2305 5 0.051988555169806716 2305 6 0.022189386692510027 2305 7 0.0508318116785631 2305 8 0.020883805306930014 2305 9 0.021790435399708485 2305 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-0.023446365738109876 1213 92-93 -0.0030759076784221406 1213 94-95 0.1359835723513143 1213 96-97 0.12425292755101225 1213 98-99 0.1082962190158554 1213 100-101 0.3415685619905986 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 7.0 9 23.0 10 23.0 11 33.0 12 80.0 13 204.0 14 527.0 15 1179.0 16 2267.0 17 3779.0 18 5659.0 19 8256.0 20 11323.0 21 15041.0 22 19534.0 23 24938.0 24 31812.0 25 40980.0 26 53523.0 27 75390.0 28 118543.0 29 165594.0 30 141454.0 31 132707.0 32 175228.0 33 247803.0 34 380578.0 35 646092.0 36 1237983.0 37 1966852.0 38 592775.0 39 520.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.243903768567485 25.20678159568271 22.10745484266932 15.441859793080484 2 28.87980032478203 25.90837422613477 31.56745603419407 13.644369414889127 3 35.92064001762419 19.645296848381673 28.0661897055538 16.367873428440344 4 27.98408870765181 19.837749019726147 31.991521672266206 20.186640600355844 5 34.79765541928173 18.421815701032685 30.188107706205198 16.592421173480385 6 29.018718650149893 19.457875947820476 34.74257983541907 16.78082556661056 7 31.07584088204859 23.25787814428721 27.46952115549886 18.196759818165336 8 27.729589373821756 29.305259210121054 25.329785547806182 17.635365868251007 9 32.116933332480976 23.557777811653633 25.789601106888107 18.535687748977292 10-11 24.517040879342076 30.077077252232225 29.152166617013876 16.253715251411826 12-13 22.679383336944937 28.5111346129448 29.09266566853082 19.716816381579445 14-15 21.879693615838296 28.834469513123643 29.635565189411654 19.650271681626407 16-17 23.1782644208286 27.292017138341507 30.275892613757716 19.253825827072173 18-19 20.680201491400915 27.410593559074382 32.388336302661315 19.520868646863388 20-21 22.802250624394844 27.701141851264122 31.912883211732673 17.58372431260836 22-23 20.839453525632358 31.683303263048035 29.664668373681934 17.81257483763767 24-25 20.676546177352886 27.379859416293883 31.77285845722471 20.170735949128517 26-27 20.657368072257853 27.642583064553012 29.437563547962558 22.26248531522658 28-29 22.345468552789026 29.687027718130704 29.374424246397957 18.593079482682313 30-31 19.936672913483633 28.016769203962756 31.386698295787685 20.659859586765926 32-33 19.971398396939897 30.087734093769786 29.679912508501065 20.260955000789252 34-35 22.342476044169963 29.884642878276242 29.72308783227911 18.049793245274685 36-37 20.230338336118972 29.724990080949777 31.84513840322675 18.199533179704506 38-39 20.800971762994013 27.47362193085688 31.16996517346594 20.555441132683168 40-41 22.602717496335252 27.70541163465307 30.77754004506704 18.91433082394464 42-43 20.37335197607151 29.864781411572732 31.17920919438866 18.582657417967102 44-45 20.11586728805029 27.758835009797668 31.493103276164085 20.63219442598796 46-47 22.306983979585272 27.700437546282085 29.30566728369285 20.686911190439798 48-49 20.356108861191917 30.041393559114994 30.818752206798628 18.783745372894465 50-51 22.721614783605133 27.884485586147278 30.331008443908125 19.062891186339463 52-53 20.567479122658668 27.751133583757337 28.343482988600766 23.33790430498323 54-55 22.65966484531338 27.93493125562727 30.19123184981765 19.2141720492417 56-57 20.49812282442876 28.16907596274304 30.318942801471838 21.013858411356363 58-59 20.477269342209894 28.183643395773718 30.609582487281873 20.72950477473452 60-61 22.276335072011435 28.448869094259084 30.465787601509405 18.809008232220076 62-63 20.123704168266258 28.26401576783101 30.463786541545133 21.148493522357594 64-65 22.177521581046435 28.039913860237885 30.37512664779426 19.407437910921427 66-67 20.330688781664755 31.422361317203638 28.57816825441145 19.668781646720152 68-69 20.17045901703852 31.83824059864374 28.515638321593933 19.475662062723803 70-71 19.896701262717745 32.49682555961271 28.265328700195674 19.341144477473872 72-73 19.92005617054137 32.29415919003588 28.11212630338077 19.673658336041985 74-75 20.17398535527209 31.60867305117408 28.340084091473432 19.877257502080397 76-77 20.082751985550864 31.279322545997506 28.4954114391274 20.142514029324225 78-79 19.89905943346982 31.18819396537026 28.65678600750722 20.2559605936527 80-81 19.77814095413569 31.236358688926064 28.451117491285544 20.534382865652706 82-83 19.519117232805865 30.93887201405034 28.58555942008983 20.956451333053963 84-85 19.670642388318065 30.694688510186285 28.824069399183 20.81059970231265 86-87 19.988952086828498 30.52571689095428 29.11743174298741 20.367899279229817 88-89 20.243712725000478 30.656133439098422 28.698096777676014 20.40205705822509 90-91 20.307092938065853 30.455237084493554 28.587836969872498 20.649833007568088 92-93 19.867864930453738 30.96973076938527 28.680132282629557 20.482272017531443 94-95 19.542174760882254 31.128678070078575 28.706270778033527 20.622876391005647 96-97 19.770198486779737 31.237450906256488 28.580363224018967 20.411987382944808 98-99 20.2856710911927 30.702087361201 28.163438278296354 20.848803269309947 100-101 20.47424534959064 30.626160798839464 27.617880105875347 21.28171374569455 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 157.0 1 93.5 2 41.0 3 85.0 4 103.0 5 73.5 6 63.5 7 66.5 8 62.5 9 67.5 10 68.5 11 74.5 12 84.5 13 94.0 14 115.0 15 135.5 16 169.5 17 220.0 18 318.5 19 500.5 20 814.0 21 1344.0 22 2164.0 23 3539.5 24 5565.0 25 8631.5 26 13644.5 27 20362.5 28 28443.0 29 37977.0 30 48911.5 31 60826.5 32 78986.0 33 108350.5 34 146337.5 35 194813.0 36 264064.0 37 336264.5 38 398144.5 39 431547.0 40 437855.5 41 426034.5 42 396221.5 43 369326.0 44 335444.5 45 301648.0 46 280356.5 47 251790.5 48 215260.0 49 185621.0 50 155352.0 51 127128.0 52 103265.0 53 81072.5 54 64873.5 55 51172.0 56 37191.5 57 25520.0 58 18005.0 59 13156.5 60 9571.5 61 6795.5 62 4632.5 63 3156.5 64 2157.5 65 1497.0 66 1024.5 67 694.0 68 495.0 69 340.0 70 232.5 71 167.0 72 113.5 73 77.5 74 53.0 75 38.5 76 37.0 77 27.5 78 17.0 79 12.0 80 7.5 81 6.0 82 3.5 83 3.0 84 2.5 85 1.5 86 1.0 87 0.5 88 0.5 89 1.5 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16757074221069787 2 0.0 3 0.0 4 0.022800636057427444 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.557196567545368E-4 12-13 1.6391542816267032E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 1.1474079971386923E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0011883868541793599 38-39 6.966405696913489E-4 40-41 2.950477706928066E-4 42-43 4.8355051307987743E-4 44-45 3.3602662773347415E-4 46-47 0.0034340282200079433 48-49 0.003950361818720355 50-51 0.004483086960249034 52-53 0.004155256103923693 54-55 0.006384505926936008 56-57 0.009589052547516214 58-59 0.017186532642855985 60-61 0.004704372788268639 62-63 0.003032435421009401 64-65 0.010170952317493693 66-67 0.02356284279838386 68-69 0.02601337844941578 70-71 0.02623466427743539 72-73 0.023349752741772388 74-75 0.02484138313805269 76-77 0.024882361995093354 78-79 0.016047320417125425 80-81 0.003475007077048611 82-83 0.0011883868541793599 84-85 0.00568786535724466 86-87 0.014957282819843667 88-89 0.016809527158081844 90-91 0.024234896053850808 92-93 0.02448076919609481 94-95 0.025693743364498575 96-97 0.027226352617819544 98-99 0.03360266277334741 100-101 0.0239152609689336 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6100707.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.51402931482311 #Duplication Level Percentage of deduplicated Percentage of total 1 91.57689003487273 50.83802157956258 2 4.309481033736484 4.784733128770427 3 1.2195143549924938 2.0310046695870274 4 0.6442853278579067 1.43067498311257 5 0.3315852276033609 0.9203816022767635 6 0.27993368329049695 0.9324148020237033 7 0.22254632686734382 0.8648110319533951 8 0.1538432296593441 0.6832366048956715 9 0.09123191243022453 0.45581859549889736 >10 0.9291637393855573 10.65529830187415 >50 0.14031328287827663 5.360860068839456 >100 0.08520162668402398 9.46652958259085 >500 0.01251707856503766 4.874422054938622 >1k 0.0033756101323582045 2.5720830686595835 >5k 0.0 0.0 >10k+ 1.1753104445860215E-4 4.12970992541625 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT 220252 3.6102700883684467 TruSeq Adapter, Index 7 (97% over 35bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 12086 0.19810818647740336 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 10457 0.17140636322970434 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 10382 0.17017699751848434 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 7.212278839157495E-4 0.0 0.0 2 7.704025123645505E-4 0.0 0.0 3 9.179263977109538E-4 0.0 1.6391542816267032E-5 4 9.507094833434878E-4 0.0 1.6391542816267032E-5 5 0.0010654502830573572 0.0 1.6391542816267032E-5 6 0.0011637995399549593 0.0 1.6391542816267032E-5 7 0.001442455767831499 0.0 1.6391542816267032E-5 8 0.0017702866241568395 0.0 1.6391542816267032E-5 9 0.0020653343948496463 0.0 3.2783085632534064E-5 10-11 0.0034094409057835425 0.0 3.2783085632534064E-5 12-13 0.0047699389595337064 0.0 3.2783085632534064E-5 14-15 0.006073066613426935 0.0 4.097885704066758E-5 16-17 0.007417173124360832 0.0 4.91746284488011E-5 18-19 0.008933390834865534 0.0 4.91746284488011E-5 20-21 0.010785635173103707 0.0 5.737039985693461E-5 22-23 0.013014884996116024 0.0 6.556617126506813E-5 24-25 0.015711293789391952 0.0 6.556617126506813E-5 26-27 0.01832574486858654 0.0 6.556617126506813E-5 28-29 0.021563074574799283 0.0 6.556617126506813E-5 30-31 0.030988211694152824 0.0 8.195771408133516E-5 32-33 0.034733679227669845 0.0 8.195771408133516E-5 34-35 0.03887254378877727 1.6391542816267032E-5 8.195771408133516E-5 36-37 0.04272455635060002 1.6391542816267032E-5 8.195771408133516E-5 38-39 0.04703553211127825 1.6391542816267032E-5 8.195771408133516E-5 40-41 0.05124815861505888 1.6391542816267032E-5 8.195771408133516E-5 42-43 0.05590335677487872 1.6391542816267032E-5 8.195771408133516E-5 44-45 0.06099293081932963 1.6391542816267032E-5 8.195771408133516E-5 46-47 0.06622183297771882 1.6391542816267032E-5 8.195771408133516E-5 48-49 0.07237685730522708 1.6391542816267032E-5 8.195771408133516E-5 50-51 0.07877775477497936 1.6391542816267032E-5 8.195771408133516E-5 52-53 0.08564581121499525 1.6391542816267032E-5 9.015348548946868E-5 54-55 0.09296463508245847 1.6391542816267032E-5 9.83492568976022E-5 56-57 0.10042278706385997 1.6391542816267032E-5 9.83492568976022E-5 58-59 0.10865953732903416 1.6391542816267032E-5 9.83492568976022E-5 60-61 0.11647830325239353 1.6391542816267032E-5 9.83492568976022E-5 62-63 0.1249281435741792 1.6391542816267032E-5 1.065450283057357E-4 64-65 0.13383694709482033 1.6391542816267032E-5 1.1474079971386923E-4 66-67 0.1441226402120279 1.6391542816267032E-5 1.1474079971386923E-4 68-69 0.15579341869721 1.6391542816267032E-5 1.1474079971386923E-4 70-71 0.16831655740883802 1.6391542816267032E-5 1.3113234253013626E-4 72-73 0.18228215188829755 1.6391542816267032E-5 1.3113234253013626E-4 74-75 0.19810818647740336 1.6391542816267032E-5 1.3113234253013626E-4 76-77 0.21598316391854255 1.6391542816267032E-5 1.3113234253013626E-4 78-79 0.23605460809706152 1.6391542816267032E-5 1.3113234253013626E-4 80-81 0.25853560906957174 1.6391542816267032E-5 1.3113234253013626E-4 82-83 0.28389332580633686 1.6391542816267032E-5 1.3113234253013626E-4 84-85 0.31333253670435246 1.6391542816267032E-5 1.475238853464033E-4 86-87 0.346328712393498 1.6391542816267032E-5 1.475238853464033E-4 88-89 0.38421776361329923 1.6391542816267032E-5 1.475238853464033E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 28150 0.0 82.619255 3 GATCGGA 28375 0.0 82.39055 1 CGGAAGA 28615 0.0 81.67509 4 ATCGGAA 28875 0.0 80.610634 2 GAAGAGC 30160 0.0 77.427376 6 GGAAGAG 34050 0.0 68.73521 5 AGAGCAC 34305 0.0 68.04431 8 GAGCACA 34385 0.0 67.99649 9 AAGAGCA 36300 0.0 64.33085 7 GCCGTCT 25620 0.0 45.559906 50-51 ATGCCGT 25565 0.0 45.480114 48-49 TATGCCG 25440 0.0 45.423534 48-49 TCGTATG 25740 0.0 45.3545 44-45 TCTCGTA 25555 0.0 45.30183 42-43 CGTATGC 25595 0.0 45.25887 46-47 TGCCGTC 25630 0.0 45.227085 50-51 CTCGTAT 25575 0.0 45.062126 44-45 TCGTATC 25230 0.0 45.017235 38-39 CGTCTTC 25890 0.0 45.013973 52-53 AATTCGT 25185 0.0 44.975468 34-35 >>END_MODULE