FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n01_35011-1.341000000358a7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n01_35011-1.341000000358a7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6100707
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT2202523.6102700883684467TruSeq Adapter, Index 7 (97% over 35bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC120860.19810818647740336No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA104570.17140636322970434No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG103820.17017699751848434No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG281500.082.6192553
GATCGGA283750.082.390551
CGGAAGA286150.081.675094
ATCGGAA288750.080.6106342
GAAGAGC301600.077.4273766
GGAAGAG340500.068.735215
AGAGCAC343050.068.044318
GAGCACA343850.067.996499
AAGAGCA363000.064.330857
GCCGTCT256200.045.55990650-51
ATGCCGT255650.045.48011448-49
TATGCCG254400.045.42353448-49
TCGTATG257400.045.354544-45
TCTCGTA255550.045.3018342-43
CGTATGC255950.045.2588746-47
TGCCGTC256300.045.22708550-51
CTCGTAT255750.045.06212644-45
TCGTATC252300.045.01723538-39
CGTCTTC258900.045.01397352-53
AATTCGT251850.044.97546834-35
Produced by FastQC (version 0.11.2)