##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n01_35010-1.341000000357eb.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6944751 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.68646147284474 33.0 33.0 33.0 33.0 33.0 2 32.73527704593008 33.0 33.0 33.0 33.0 33.0 3 32.58462484832069 33.0 33.0 33.0 33.0 33.0 4 36.63259978651502 37.0 37.0 37.0 37.0 37.0 5 36.69182725197779 37.0 37.0 37.0 37.0 37.0 6 36.578503246552685 37.0 37.0 37.0 37.0 37.0 7 36.5231876564041 37.0 37.0 37.0 37.0 37.0 8 36.47188300919644 37.0 37.0 37.0 37.0 37.0 9 36.487278953557876 37.0 37.0 37.0 37.0 37.0 10-11 36.442282019902514 37.0 37.0 37.0 37.0 37.0 12-13 36.42630405323388 37.0 37.0 37.0 37.0 37.0 14-15 38.314024793689505 40.0 37.0 40.0 37.0 40.0 16-17 38.31263892686721 40.0 37.0 40.0 37.0 40.0 18-19 38.32183551289312 40.0 37.0 40.0 37.0 40.0 20-21 38.25895161683982 40.0 37.0 40.0 37.0 40.0 22-23 38.154314531939306 40.0 37.0 40.0 37.0 40.0 24-25 38.11821122168383 40.0 37.0 40.0 37.0 40.0 26-27 38.067176490560996 40.0 37.0 40.0 37.0 40.0 28-29 37.93049909204808 40.0 37.0 40.0 33.0 40.0 30-31 37.89119228320785 40.0 37.0 40.0 33.0 40.0 32-33 37.78331490934664 40.0 37.0 40.0 33.0 40.0 34-35 37.68948231549267 40.0 37.0 40.0 33.0 40.0 36-37 37.59030014179054 40.0 37.0 40.0 33.0 40.0 38-39 37.52585780253317 40.0 37.0 40.0 33.0 40.0 40-41 37.482415136266226 40.0 37.0 40.0 33.0 40.0 42-43 37.3370011394217 40.0 37.0 40.0 33.0 40.0 44-45 37.229789304180954 40.0 37.0 40.0 33.0 40.0 46-47 37.17178564069468 40.0 37.0 40.0 33.0 40.0 48-49 37.006836818195495 38.5 37.0 40.0 33.0 40.0 50-51 36.86515060079188 37.0 37.0 40.0 33.0 40.0 52-53 36.74258969112068 37.0 37.0 40.0 33.0 40.0 54-55 36.607473687681534 37.0 37.0 40.0 33.0 40.0 56-57 36.44240283056945 37.0 37.0 40.0 33.0 40.0 58-59 36.246870910130546 37.0 37.0 40.0 33.0 40.0 60-61 35.986546961870914 37.0 37.0 40.0 33.0 40.0 62-63 35.762052231966265 37.0 37.0 40.0 30.0 40.0 64-65 35.51827020148022 37.0 33.0 40.0 27.0 40.0 66-67 35.20642698348725 37.0 33.0 38.5 27.0 40.0 68-69 34.94824803653867 37.0 33.0 37.0 27.0 40.0 70-71 34.650936729049036 37.0 33.0 37.0 27.0 40.0 72-73 34.292626258306456 37.0 33.0 37.0 27.0 40.0 74-75 33.912104768047115 37.0 33.0 37.0 27.0 38.5 76-77 31.312906611050565 33.0 30.0 35.0 24.5 37.0 78-79 33.58073723593546 37.0 33.0 37.0 27.0 37.0 80-81 33.88816755273155 37.0 33.0 37.0 27.0 37.0 82-83 33.79692763642642 37.0 33.0 37.0 27.0 37.0 84-85 33.63163805296979 37.0 33.0 37.0 27.0 37.0 86-87 33.46880068126272 37.0 33.0 37.0 27.0 37.0 88-89 33.33653287209289 37.0 33.0 37.0 27.0 37.0 90-91 33.19604964958427 37.0 33.0 37.0 27.0 37.0 92-93 33.03400150703747 37.0 33.0 37.0 27.0 37.0 94-95 32.92016495623817 35.0 33.0 37.0 27.0 37.0 96-97 32.79138812896244 33.0 33.0 37.0 27.0 37.0 98-99 32.634955090542476 33.0 33.0 37.0 27.0 37.0 100-101 30.976639047245897 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 0.14492791824827123 2306 2 0.12916884290170572 2306 3 0.31758580613079346 2306 4 0.20890719726823903 2306 5 0.13456853077862263 2306 6 0.18920554504391163 2306 7 0.2045967602041685 2306 8 0.25546111694303164 2306 9 0.1975563109570686 2306 10-11 0.23481219226696481 2306 12-13 0.21738051262763491 2306 14-15 0.3856034762623963 2306 16-17 0.4209261980698358 2306 18-19 0.4290153750014909 2306 20-21 0.43992898841370476 2306 22-23 0.49490908953982427 2306 24-25 0.450873428049654 2306 26-27 0.4568106463426318 2306 28-29 0.5121341826002848 2306 30-31 0.44146836363830744 2306 32-33 0.5626097341237966 2306 34-35 0.5011611152055266 2306 36-37 0.47768577491908815 2306 38-39 0.5205951538762221 2306 40-41 0.520448025558089 2306 42-43 0.5488707746936896 2306 44-45 0.5326826084378169 2306 46-47 0.5526864490817474 2306 48-49 0.5560002103014057 2306 50-51 0.5082124443824014 2306 52-53 0.49219822921374146 2306 54-55 0.5740830335575922 2306 56-57 0.5824817586716904 2306 58-59 0.5750000494067891 2306 60-61 0.5443627530361113 2306 62-63 0.5497835579450978 2306 64-65 0.5282146522193685 2306 66-67 0.42537022559238835 2306 68-69 0.46319466271296506 2306 70-71 0.5088699310827991 2306 72-73 0.5162237883306489 2306 74-75 0.37817654813034096 2306 76-77 0.3722884279542029 2306 78-79 0.4380021836955592 2306 80-81 0.5432160528131149 2306 82-83 0.4917437599566554 2306 84-85 0.5803289216220051 2306 86-87 0.5632144184980845 2306 88-89 0.6291769039532227 2306 90-91 0.6129595433915895 2306 92-93 0.5700031203271294 2306 94-95 0.6206741157905284 2306 96-97 0.5924682144674449 2306 98-99 0.5334844703094959 2306 100-101 0.45919680836709276 2307 1 0.13276073734481741 2307 2 0.1355471581793637 2307 3 0.34577367243841195 2307 4 0.22320188938044794 2307 5 0.12709163699835813 2307 6 0.15694172091289005 2307 7 0.14495232394772017 2307 8 0.17679700768608342 2307 9 0.11867456934727727 2307 10-11 0.18187092717561626 2307 12-13 0.15015277689942508 2307 14-15 0.36999347722659337 2307 16-17 0.32408970126200387 2307 18-19 0.3615290286767916 2307 20-21 0.33529164587002214 2307 22-23 0.3256960612131863 2307 24-25 0.3572564351640253 2307 26-27 0.38358510759925224 2307 28-29 0.38111683743949953 2307 30-31 0.3863750090008793 2307 32-33 0.4243873204019337 2307 34-35 0.3495611988837979 2307 36-37 0.3984175809232653 2307 38-39 0.42305027581232224 2307 40-41 0.42775256966652364 2307 42-43 0.4625208773114693 2307 44-45 0.32831278667596564 2307 46-47 0.40391885254182824 2307 48-49 0.3692261106175252 2307 50-51 0.287016017409762 2307 52-53 0.3552654679688274 2307 54-55 0.3074224074513552 2307 56-57 0.43272147278818096 2307 58-59 0.41487360225494996 2307 60-61 0.3598838223960854 2307 62-63 0.38254691203155033 2307 64-65 0.3283427953195286 2307 66-67 0.22801069660114592 2307 68-69 0.2739679807110207 2307 70-71 0.26227831371629406 2307 72-73 0.14817269982452075 2307 74-75 0.08433477752585361 2307 76-77 -0.14654865081355695 2307 78-79 0.1122741311607598 2307 80-81 0.20004865840956398 2307 82-83 0.2596311507100353 2307 84-85 0.3743417505671047 2307 86-87 0.359438307781069 2307 88-89 0.38958735788196464 2307 90-91 0.28616330168193116 2307 92-93 0.20297640182356247 2307 94-95 0.2972000388340774 2307 96-97 0.3621469829162578 2307 98-99 0.13133345687276687 2307 100-101 0.06852719287387288 2201 1 0.12077788973044079 2201 2 0.07973120463967831 2201 3 0.283800748155528 2201 4 0.20913822466843612 2201 5 0.09508654151978391 2201 6 0.13414742141829095 2201 7 0.14177243169194043 2201 8 0.13755076513519526 2201 9 0.10195547487360557 2201 10-11 0.1429715269937688 2201 12-13 0.11863661593800856 2201 14-15 0.28103021758094116 2201 16-17 0.29734735038802285 2201 18-19 0.3355215615575631 2201 20-21 0.31076880556990005 2201 22-23 0.3294889790291151 2201 24-25 0.29198181970991754 2201 26-27 0.2506398327767201 2201 28-29 0.3660583103337913 2201 30-31 0.3845233651383779 2201 32-33 0.3303089015020291 2201 34-35 0.32214444875865667 2201 36-37 0.32784876338997293 2201 38-39 0.2906279617451588 2201 40-41 0.3094101825611162 2201 42-43 0.3553075005499622 2201 44-45 0.39395475508870703 2201 46-47 0.35463866358198715 2201 48-49 0.3011804907981741 2201 50-51 0.36279458397800823 2201 52-53 0.40997209105556465 2201 54-55 0.4319962989646413 2201 56-57 0.44797141230368 2201 58-59 0.4282166299704855 2201 60-61 0.4019156717454564 2201 62-63 0.45653406236453264 2201 64-65 0.4253569553721519 2201 66-67 0.35127609944137816 2201 68-69 0.25825192915522166 2201 70-71 0.3495529867107052 2201 72-73 0.3114735089218428 2201 74-75 0.3183153747452252 2201 76-77 0.16754729732934948 2201 78-79 0.3637218468225001 2201 80-81 0.43579923256776 2201 82-83 0.5843862625694172 2201 84-85 0.5975306753861958 2201 86-87 0.5800099786179587 2201 88-89 0.6089056301457774 2201 90-91 0.4525896440446999 2201 92-93 0.3881156932289258 2201 94-95 0.4543282472494852 2201 96-97 0.5087225127474682 2201 98-99 0.5382635148137993 2201 100-101 0.410570321071539 1101 1 0.14691947486866752 1101 2 0.14207217689017426 1101 3 0.30170841529574943 1101 4 0.19061650978715505 1101 5 0.13238230691753472 1101 6 0.14907016708061605 1101 7 0.1054753755086324 1101 8 0.1286127920484006 1101 9 0.16477125915498902 1101 10-11 0.09294690028170294 1101 12-13 0.11796233058257144 1101 14-15 0.23734602493701118 1101 16-17 0.19043874225211965 1101 18-19 0.16747229395565455 1101 20-21 0.21405700240229208 1101 22-23 0.25308398276921196 1101 24-25 0.21586508683915895 1101 26-27 0.20496668898224613 1101 28-29 0.2691522251962226 1101 30-31 0.27884478581665206 1101 32-33 0.24305103836310593 1101 34-35 0.32746496066345543 1101 36-37 0.26404982605667726 1101 38-39 0.2911414583183145 1101 40-41 0.32720729174995 1101 42-43 0.2970942996369885 1101 44-45 0.26427557686366754 1101 46-47 0.3441638884276159 1101 48-49 0.27684266866165075 1101 50-51 0.28517806876821084 1101 52-53 0.27223750582528083 1101 54-55 0.3426111082237142 1101 56-57 0.3464155382734333 1101 58-59 0.33122893315862 1101 60-61 0.3235073675544129 1101 62-63 0.28739233684490983 1101 64-65 0.12779359462095385 1101 66-67 0.18094965310223898 1101 68-69 0.20675075589118563 1101 70-71 0.17517196713367866 1101 72-73 0.23015357085863997 1101 74-75 0.10269539794019522 1101 76-77 -0.14339686936568796 1101 78-79 0.20644242022873271 1101 80-81 0.31345781923113947 1101 82-83 0.3548800746000609 1101 84-85 0.3358056340853892 1101 86-87 0.3102152093337551 1101 88-89 0.38167930609705536 1101 90-91 0.28326034951361834 1101 92-93 0.34768513731684436 1101 94-95 0.382240399362054 1101 96-97 0.2613931693102529 1101 98-99 0.23156610888356255 1101 100-101 0.19958468440399457 2304 1 0.14201204255189737 2304 2 0.08253911442471207 2304 3 0.3085085178771081 2304 4 0.16542890109943897 2304 5 0.08678810659220204 2304 6 0.10878143243139249 2304 7 0.0833360322851533 2304 8 0.1258302134507545 2304 9 0.058029003661047796 2304 10-11 0.05589760893978024 2304 12-13 0.09417543703400355 2304 14-15 0.21263028535783945 2304 16-17 0.20782385512480062 2304 18-19 0.20600913679223964 2304 20-21 0.17951921659694392 2304 22-23 0.2299341028989872 2304 24-25 0.2684313632407722 2304 26-27 0.22651791106648744 2304 28-29 0.2253386751147346 2304 30-31 0.21157426262832502 2304 32-33 0.28378272338627397 2304 34-35 0.31386454710631284 2304 36-37 0.3008167108866644 2304 38-39 0.26429961465344576 2304 40-41 0.2720288713329069 2304 42-43 0.3511178622500424 2304 44-45 0.3827351778242445 2304 46-47 0.37213736193280766 2304 48-49 0.3033301135545443 2304 50-51 0.25580262975729084 2304 52-53 0.21605856772442422 2304 54-55 0.3377297252105578 2304 56-57 0.3882210477284005 2304 58-59 0.25447566193516735 2304 60-61 0.26772147737693786 2304 62-63 0.2737904965874449 2304 64-65 0.25956735534497 2304 66-67 0.19311899005496969 2304 68-69 0.16833660928224248 2304 70-71 0.13554246421498561 2304 72-73 0.08015687150578543 2304 74-75 0.06291144268895721 2304 76-77 -0.21728840416917805 2304 78-79 0.07063088920071436 2304 80-81 0.12022217735882634 2304 82-83 0.1254533577353456 2304 84-85 0.1367705996796218 2304 86-87 0.2906106156408512 2304 88-89 0.39589898774539023 2304 90-91 0.32979492168450975 2304 92-93 0.32265675800319826 2304 94-95 0.31499045913117385 2304 96-97 0.2472804260646626 2304 98-99 0.2158349584722501 2304 100-101 0.11775644777320693 2202 1 0.08406078825394303 2202 2 0.04003650229235234 2202 3 0.2752878872936009 2202 4 0.1460169615951159 2202 5 0.050303706975832085 2202 6 0.08342785479299408 2202 7 0.1008077295782357 2202 8 0.06862134473873738 2202 9 0.05004066200188362 2202 10-11 0.06646718045084299 2202 12-13 0.07182278301787193 2202 14-15 -0.009709584015375583 2202 16-17 0.04238047799201894 2202 18-19 0.05983174647676748 2202 20-21 0.08372583998791328 2202 22-23 0.13731828748323238 2202 24-25 0.1568060373099982 2202 26-27 0.09188555239243357 2202 28-29 0.12670821620495332 2202 30-31 0.07291958717907931 2202 32-33 0.10306861838222403 2202 34-35 0.04151779068135397 2202 36-37 0.05283608818053409 2202 38-39 0.08695002375765881 2202 40-41 0.053257574173613875 2202 42-43 0.07255347783364385 2202 44-45 0.05316881660493067 2202 46-47 0.07215949857394577 2202 48-49 0.09973896945290761 2202 50-51 0.061975111071653544 2202 52-53 0.12890318182218863 2202 54-55 0.13252854597087804 2202 56-57 0.12460333559508996 2202 58-59 0.04511922710607763 2202 60-61 0.09607180317205177 2202 62-63 0.07977306531005723 2202 64-65 -0.026595389793527602 2202 66-67 -0.033900403923361466 2202 68-69 -0.02492307220488499 2202 70-71 0.06917821267646929 2202 72-73 0.10683822687408195 2202 74-75 -0.023288591475001397 2202 76-77 -0.20002463867632514 2202 78-79 0.12985918598537438 2202 80-81 0.09747299919867913 2202 82-83 0.2503484089033705 2202 84-85 0.16580661003767716 2202 86-87 0.27261956761844175 2202 88-89 0.27844199598347075 2202 90-91 0.18583846410071914 2202 92-93 0.301909801167497 2202 94-95 0.24556269551197119 2202 96-97 0.33805661377061114 2202 98-99 0.19745538033470922 2202 100-101 0.11134456923242197 1102 1 0.07508247943020763 1102 2 0.049214124470992715 1102 3 0.25906259051756564 1102 4 0.15972473893640426 1102 5 0.031394986435294925 1102 6 0.049008790614550435 1102 7 0.07884523400662857 1102 8 0.06182996897165083 1102 9 0.05992590363021577 1102 10-11 0.07643415196263703 1102 12-13 0.07828111426313455 1102 14-15 0.0382098091876415 1102 16-17 0.12562096547152635 1102 18-19 0.0841821996539025 1102 20-21 0.1272088677549661 1102 22-23 0.1738022059674904 1102 24-25 0.11272817950538183 1102 26-27 0.1442688123630731 1102 28-29 0.22939206369367326 1102 30-31 0.15436912003534786 1102 32-33 0.17121929493264076 1102 34-35 0.18582436721536055 1102 36-37 0.1227274292647067 1102 38-39 0.1029868311597042 1102 40-41 0.20467371348696872 1102 42-43 0.07420518744272186 1102 44-45 0.10246709262589349 1102 46-47 0.1531538945766684 1102 48-49 0.09880597489252096 1102 50-51 0.12118284347372565 1102 52-53 0.06303272556164785 1102 54-55 0.1266245600867606 1102 56-57 0.1724481153086117 1102 58-59 0.1334388370711892 1102 60-61 0.10144238544980766 1102 62-63 0.08604931736827126 1102 64-65 0.03640763502701816 1102 66-67 0.05864711817406487 1102 68-69 0.056521883886553326 1102 70-71 -0.04238172016759023 1102 72-73 -0.05869033283106262 1102 74-75 -0.022224812803983696 1102 76-77 -0.07973653010818182 1102 78-79 0.059304730075659506 1102 80-81 0.04778410920158649 1102 82-83 0.06465477276040588 1102 84-85 0.0029119988618333537 1102 86-87 0.12088551291092386 1102 88-89 0.27278079592966264 1102 90-91 0.10243344396282339 1102 92-93 0.1453564810016701 1102 94-95 0.16512809081311985 1102 96-97 0.15898842190318163 1102 98-99 0.12325024147797592 1102 100-101 -0.05859976725741234 2305 1 0.0928667708439761 2305 2 0.038526620506992515 2305 3 0.2538434462699115 2305 4 0.11802221813855596 2305 5 0.04053193948881528 2305 6 0.051967050010468085 2305 7 0.040081653949577856 2305 8 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96-97 0.05059013838734927 1213 98-99 0.06658153209239259 1213 100-101 0.24323170895754487 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 7.0 10 11.0 11 20.0 12 65.0 13 179.0 14 587.0 15 1538.0 16 3076.0 17 5017.0 18 6431.0 19 8157.0 20 10519.0 21 13488.0 22 17689.0 23 22558.0 24 29065.0 25 36787.0 26 47419.0 27 61601.0 28 85133.0 29 114248.0 30 126842.0 31 145971.0 32 197288.0 33 281938.0 34 437130.0 35 745280.0 36 1411042.0 37 2305113.0 38 830148.0 39 402.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.99861531305103 27.67482934815141 20.147743212690077 16.178812126107477 2 30.666787045352674 24.625274541880625 30.16422043065331 14.543717982113396 3 35.78670839797453 21.102413248807082 25.33852210001633 17.77235625320206 4 29.644404148846306 21.336082331684462 30.06497785222382 18.954535667245416 5 30.621184258442096 19.679582464511686 31.451610000128156 18.247623276918063 6 27.5588714411791 20.094312956648842 33.732742901797344 18.614072700374713 7 31.945090615919852 21.265298064682234 26.296795954239393 20.49281536515852 8 29.204459598335493 27.68069006361783 23.53393231809175 19.580918019954925 9 28.044446805940197 24.83308616824419 26.620594460478138 20.501872565337475 10-11 22.734949889783515 29.30144340145706 30.86376217170934 17.099844537050082 12-13 22.134130098275676 28.22514849346629 30.758796212966626 18.88192519529141 14-15 21.63167549131711 28.39122669768866 31.313332904232276 18.663764906761955 16-17 21.89135362808544 27.767367037349505 31.956458914077697 18.384820420487358 18-19 20.939829232178376 27.81124910021972 32.533635835179695 18.71528583242221 20-21 21.63092672437068 28.175265031100466 31.911432101741298 18.28237614278755 22-23 21.081634172341097 29.71951046193017 30.682755940421767 18.51609942530697 24-25 21.07193620044837 28.124384877153986 31.413386887449242 19.39029203494841 26-27 21.081287459057197 28.28609788489116 30.666039910153703 19.966574745897944 28-29 21.709370670736124 29.106613448474622 30.384796096846205 18.799219783943048 30-31 20.858523221350918 28.68484413624045 30.990758343963666 19.46587429844497 32-33 20.762695451571986 29.375660840827845 30.353456876999623 19.50818683060055 34-35 21.558317929613317 29.234475073332362 30.495924187922647 18.711282809131674 36-37 21.44201389366373 29.07822992186893 30.719731869439855 18.76002431502749 38-39 21.07070857319518 28.507340731818946 31.544076825675717 18.877873869310154 40-41 21.003177949322723 28.672468188108102 29.915115490451033 20.40923837211814 42-43 20.792548116452313 29.185514335075567 30.72796086347112 19.293976685000995 44-45 20.656719981228918 28.412138103946656 30.967941065489196 19.963200849335223 46-47 21.383371394480864 28.493445269922628 30.1005381648023 20.02264517079421 48-49 20.857309463965947 29.458224707100666 30.288234843909063 19.39623098502432 50-51 21.572862315741453 28.806281962556017 30.054882644481477 19.565973077221045 52-53 20.821514274274556 28.7486652146237 29.46092994733866 20.968890563763086 54-55 21.421178967080063 28.896086924402965 30.00051264822698 19.682221460289995 56-57 20.69518530215114 28.822116530442933 30.21025358106626 20.272444586339667 58-59 20.7074628993687 28.91288615512237 30.318774874471003 20.060876071037924 60-61 21.16761423609181 28.957298751777323 30.278241538475275 19.59684547365559 62-63 20.320167313780225 28.84858089929861 30.307111764444567 20.524140022476598 64-65 21.061571091632853 28.72174662638655 30.211024190823842 20.005658091156757 66-67 20.541648021322736 29.7921894504248 29.60193132877223 20.06423119948024 68-69 20.462134475886945 30.031136199050444 29.513805472066718 19.992923852995894 70-71 20.265538799387674 30.327261980043108 29.37182698209445 20.035372238474768 72-73 20.273807940388505 30.207657839668013 29.370755619381168 20.14777860056232 74-75 20.380571159197768 30.064342384706062 29.388771469995962 20.166314986100208 76-77 20.431729861227783 29.891574597024356 29.42064448881644 20.256051052931422 78-79 20.319621666200177 29.816029890075324 29.615171832865418 20.24917661085908 80-81 20.214322747562417 30.001663900339352 29.539537360826547 20.24447599127169 82-83 20.095347881315387 29.90645565893693 29.490943024039503 20.50725343570818 84-85 20.236802997531818 29.62212609333015 29.652805578035764 20.488265331102273 86-87 20.306634580350444 29.615129821408253 29.740040352719056 20.338195245522243 88-89 20.563420991784493 29.836250765179134 29.364545417166553 20.23578282586982 90-91 20.59888455921711 29.728426096737167 29.30170486137141 20.370984482674316 92-93 20.31710221670824 29.884358776063706 29.248055873154506 20.55048313407355 94-95 20.08359001672751 29.901891876399063 29.218122169293224 20.796395937580208 96-97 20.10536694323161 30.224488077840412 29.06891707581527 20.601227903112708 98-99 20.38549851257673 30.172387841170018 28.898992775413852 20.543120870839402 100-101 20.47089997613483 29.998460358110044 28.68271466086183 20.847925004893295 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 133.0 1 78.0 2 24.0 3 30.5 4 35.5 5 34.0 6 37.0 7 56.5 8 69.0 9 81.5 10 98.5 11 111.0 12 128.5 13 142.0 14 165.5 15 208.0 16 262.0 17 318.5 18 402.0 19 512.5 20 726.5 21 1225.5 22 2117.0 23 3671.5 24 6016.5 25 9912.0 26 16865.0 27 26457.0 28 38370.0 29 52548.5 30 68850.5 31 87807.0 32 113588.0 33 149229.5 34 191910.5 35 239378.5 36 294696.0 37 356096.5 38 412628.5 39 446922.0 40 464629.5 41 460161.5 42 433521.0 43 410313.0 44 380419.5 45 350186.0 46 331513.0 47 302297.5 48 259801.0 49 220469.5 50 180860.5 51 144644.5 52 116619.0 53 92879.5 54 75446.5 55 58946.0 56 41605.5 57 28339.0 58 20201.0 59 14904.0 60 10814.5 61 7639.0 62 5184.5 63 3450.0 64 2370.5 65 1672.5 66 1141.0 67 779.5 68 548.0 69 407.5 70 283.5 71 201.5 72 158.5 73 113.0 74 85.0 75 69.0 76 61.0 77 43.5 78 24.5 79 17.5 80 18.0 81 13.5 82 8.0 83 5.0 84 2.0 85 1.0 86 2.0 87 1.5 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1695669146381202 2 0.0 3 7.199682177229968E-5 4 0.02326937279680726 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.367939613673694E-4 12-13 1.4399364354459937E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 2.8798728708919874E-5 28-29 1.151949148356795E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0010727526444072653 38-39 5.975736207100874E-4 40-41 3.023866514436587E-4 42-43 5.327764811150177E-4 44-45 3.6718379103872843E-4 46-47 0.003225457615399026 48-49 0.0037078363212734336 50-51 0.004276611213274601 52-53 0.004067820430134933 54-55 0.006018934300164253 56-57 0.009503580473943559 58-59 0.01635767790666649 60-61 0.0045645985003638 62-63 0.002800676366942458 64-65 0.010266746784729936 66-67 0.02234781347812182 68-69 0.02457971495306311 70-71 0.02499009683716522 72-73 0.021980629687083093 74-75 0.023910144510580725 76-77 0.024226930526378844 78-79 0.014996937975170023 80-81 0.0032326572975762556 82-83 0.0011015513731161852 84-85 0.005342164175504637 86-87 0.014125776431725199 88-89 0.01606249093740006 90-91 0.02270059790480609 92-93 0.02297418582754083 94-95 0.024435721309518512 96-97 0.02558047077569808 98-99 0.03179379649464754 100-101 0.02272219695133778 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6944751.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.59478838476753 #Duplication Level Percentage of deduplicated Percentage of total 1 91.40931528542562 59.04565377258564 2 4.817823522129853 6.224125817742664 3 1.2269747010597452 2.3776851350525288 4 0.5562077169024234 1.4371247908514688 5 0.3404715121179272 1.0996342638149659 6 0.27141677989542845 1.0519265676852136 7 0.16589052789249253 0.750096448097705 8 0.14524086899323332 0.7505442557950118 9 0.08959261795287818 0.520849457775314 >10 0.814179817557737 10.442697193628499 >50 0.0967418852858582 4.364032380346263 >100 0.0603103904713286 8.004595477867976 >500 0.0049208860027434905 2.063649270799168 >1k 8.911891566104488E-4 0.7619574812123483 >5k 0.0 0.0 >10k+ 2.229915603963277E-5 1.1054276867452422 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT 76744 1.1050648180186733 TruSeq Adapter, Index 7 (97% over 38bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0012671440631924745 0.0 0.0 2 0.001367939613673694 0.0 0.0 3 0.0014543357998004537 0.0 0.0 4 0.0015263326215727534 0.0 0.0 5 0.0017711218155985723 0.0 0.0 6 0.002030310373978851 0.0 0.0 7 0.0022319014749412904 0.0 0.0 8 0.0026638824055750885 0.0 0.0 9 0.0030382658787910465 0.0 0.0 10-11 0.004974980384465908 0.0 0.0 12-13 0.007185282812875509 0.0 0.0 14-15 0.010655529622300354 0.0 1.4399364354459937E-5 16-17 0.015349722401854293 0.0 1.4399364354459937E-5 18-19 0.02033190246849743 0.0 1.4399364354459937E-5 20-21 0.02273659631569224 0.0 1.4399364354459937E-5 22-23 0.02529248348860888 0.0 1.4399364354459937E-5 24-25 0.02810035953772857 0.0 1.4399364354459937E-5 26-27 0.030519452749277837 0.0 1.4399364354459937E-5 28-29 0.03386010527951254 7.1996821772299685E-6 2.8798728708919874E-5 30-31 0.05311205542142548 1.4399364354459937E-5 4.319809306337981E-5 32-33 0.05794304216234679 1.4399364354459937E-5 4.319809306337981E-5 34-35 0.062068460049899556 1.4399364354459937E-5 6.479713959506971E-5 36-37 0.0667410537829218 1.4399364354459937E-5 7.199682177229968E-5 38-39 0.07204001986536307 1.4399364354459937E-5 8.639618612675963E-5 40-41 0.07735338531215878 1.4399364354459937E-5 8.639618612675963E-5 42-43 0.08348031484498147 1.4399364354459937E-5 8.639618612675963E-5 44-45 0.09003922530843798 1.4399364354459937E-5 8.639618612675963E-5 46-47 0.09739010081138978 1.4399364354459937E-5 8.639618612675963E-5 48-49 0.1049857655083674 1.4399364354459937E-5 8.639618612675963E-5 50-51 0.11326540001218185 1.4399364354459937E-5 8.639618612675963E-5 52-53 0.12279777921483434 1.4399364354459937E-5 8.639618612675963E-5 54-55 0.13232295873530958 1.4399364354459937E-5 1.0079555048121957E-4 56-57 0.14262570393092566 2.8798728708919874E-5 1.151949148356795E-4 58-59 0.15405159954618963 2.8798728708919874E-5 1.151949148356795E-4 60-61 0.16685983413948174 2.8798728708919874E-5 1.2239459701290947E-4 62-63 0.18107920643951092 2.8798728708919874E-5 1.367939613673694E-4 64-65 0.19737208720658234 2.8798728708919874E-5 1.4399364354459937E-4 66-67 0.21480251775765613 2.8798728708919874E-5 1.5119332572182932E-4 68-69 0.23567439638944576 2.8798728708919874E-5 1.7279237225351925E-4 70-71 0.25859098475956876 2.8798728708919874E-5 1.7279237225351925E-4 72-73 0.28352348413931616 2.8798728708919874E-5 1.799920544307492E-4 74-75 0.31248060585613513 2.8798728708919874E-5 1.9439141878520912E-4 76-77 0.3455271470496206 2.8798728708919874E-5 2.30389829671359E-4 78-79 0.3828071013633174 4.319809306337981E-5 2.30389829671359E-4 80-81 0.42630758107814093 4.319809306337981E-5 2.30389829671359E-4 82-83 0.4737462869439092 4.319809306337981E-5 2.3758951184858898E-4 84-85 0.528406274033439 4.319809306337981E-5 2.4478919402581893E-4 86-87 0.5903811382150346 4.319809306337981E-5 2.4478919402581893E-4 88-89 0.660052462644089 4.319809306337981E-5 2.4478919402581893E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTCG 25 0.0049875206 57.100277 1 CGGAAGA 15335 0.0 55.13447 4 TCGGAAG 15240 0.0 54.698967 3 GATCGGA 16030 0.0 52.036175 1 ATCGGAA 16060 0.0 51.96527 2 GAAGAGC 17880 0.0 47.491405 6 TATGCCG 9945 0.0 42.146896 48-49 TGCCGTC 10070 0.0 41.837475 50-51 CGTATGC 10090 0.0 41.70537 46-47 GCCGTCT 10145 0.0 41.64523 50-51 GTCGCGC 80 2.2337736E-7 41.635616 1 ATGCCGT 10155 0.0 41.462406 48-49 CTCGTAT 10070 0.0 41.198345 44-45 TCTCGTA 10210 0.0 40.795948 42-43 TCGTATG 10410 0.0 40.582756 44-45 CATCTCG 10185 0.0 40.07974 40-41 CCGTCTT 10575 0.0 39.88692 52-53 ATCTCGT 10320 0.0 39.69379 42-43 GTATGCC 10800 0.0 39.07357 46-47 CGTCTTC 10855 0.0 38.967453 52-53 >>END_MODULE