FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n01_35010-1.341000000357eb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n01_35010-1.341000000357eb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6944751
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT767441.1050648180186733TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTCG250.004987520657.1002771
CGGAAGA153350.055.134474
TCGGAAG152400.054.6989673
GATCGGA160300.052.0361751
ATCGGAA160600.051.965272
GAAGAGC178800.047.4914056
TATGCCG99450.042.14689648-49
TGCCGTC100700.041.83747550-51
CGTATGC100900.041.7053746-47
GCCGTCT101450.041.6452350-51
GTCGCGC802.2337736E-741.6356161
ATGCCGT101550.041.46240648-49
CTCGTAT100700.041.19834544-45
TCTCGTA102100.040.79594842-43
TCGTATG104100.040.58275644-45
CATCTCG101850.040.0797440-41
CCGTCTT105750.039.8869252-53
ATCTCGT103200.039.6937942-43
GTATGCC108000.039.0735746-47
CGTCTTC108550.038.96745352-53
Produced by FastQC (version 0.11.2)