##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n01_35008-2.341000000358b3.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5866971 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.672908047440494 33.0 33.0 33.0 33.0 33.0 2 32.72275233676798 33.0 33.0 33.0 33.0 33.0 3 32.56357582132245 33.0 33.0 33.0 33.0 33.0 4 36.61415456118669 37.0 37.0 37.0 37.0 37.0 5 36.67756632851943 37.0 37.0 37.0 37.0 37.0 6 36.56071881043898 37.0 37.0 37.0 37.0 37.0 7 36.48206135670348 37.0 37.0 37.0 37.0 37.0 8 36.42570092812799 37.0 37.0 37.0 37.0 37.0 9 36.46523904754259 37.0 37.0 37.0 37.0 37.0 10-11 36.40066049414595 37.0 37.0 37.0 37.0 37.0 12-13 36.37512380749794 37.0 37.0 37.0 37.0 37.0 14-15 38.22504236001848 40.0 37.0 40.0 37.0 40.0 16-17 38.22662861295888 40.0 37.0 40.0 37.0 40.0 18-19 38.20761164491865 40.0 37.0 40.0 37.0 40.0 20-21 38.168365669439986 40.0 37.0 40.0 37.0 40.0 22-23 38.02866641065722 40.0 37.0 40.0 35.0 40.0 24-25 38.008275138908985 40.0 37.0 40.0 33.0 40.0 26-27 37.9542169204518 40.0 37.0 40.0 33.0 40.0 28-29 37.77376059980524 40.0 37.0 40.0 33.0 40.0 30-31 37.746171491217524 40.0 37.0 40.0 33.0 40.0 32-33 37.62315818503279 40.0 37.0 40.0 33.0 40.0 34-35 37.48283858911182 40.0 37.0 40.0 33.0 40.0 36-37 37.284075547671875 40.0 37.0 40.0 33.0 40.0 38-39 37.32606782954952 40.0 37.0 40.0 33.0 40.0 40-41 37.183944491970394 40.0 37.0 40.0 33.0 40.0 42-43 37.02179139798032 40.0 37.0 40.0 33.0 40.0 44-45 36.979989418730725 40.0 37.0 40.0 33.0 40.0 46-47 36.94093971147974 38.5 37.0 40.0 33.0 40.0 48-49 36.72237352119177 37.0 37.0 40.0 33.0 40.0 50-51 36.61560334966714 37.0 37.0 40.0 33.0 40.0 52-53 36.52076932031878 37.0 37.0 40.0 33.0 40.0 54-55 36.319979679463216 37.0 37.0 40.0 33.0 40.0 56-57 36.167796466012874 37.0 37.0 40.0 33.0 40.0 58-59 35.94329987313726 37.0 37.0 40.0 27.0 40.0 60-61 35.70419011786491 37.0 37.0 40.0 27.0 40.0 62-63 35.504193390422415 37.0 33.0 40.0 27.0 40.0 64-65 35.256167876064154 37.0 33.0 40.0 27.0 40.0 66-67 34.847758579341885 37.0 33.0 40.0 27.0 40.0 68-69 34.62041153774239 37.0 33.0 37.0 27.0 40.0 70-71 34.2678128969787 37.0 33.0 37.0 27.0 40.0 72-73 33.73191515690124 37.0 33.0 37.0 27.0 40.0 74-75 33.18477013095855 37.0 33.0 37.0 22.0 40.0 76-77 30.671070301864454 33.0 30.0 35.0 22.0 37.0 78-79 32.76374555115408 37.0 33.0 37.0 22.0 37.0 80-81 33.000414864842526 37.0 33.0 37.0 22.0 37.0 82-83 32.857458814778525 37.0 33.0 37.0 22.0 37.0 84-85 32.65389619277137 37.0 33.0 37.0 22.0 37.0 86-87 32.46527731260304 37.0 33.0 37.0 22.0 37.0 88-89 32.30198930589566 37.0 33.0 37.0 22.0 37.0 90-91 32.13425922848434 35.0 33.0 37.0 22.0 37.0 92-93 31.95383196883025 33.0 33.0 37.0 22.0 37.0 94-95 31.81055019702671 33.0 33.0 37.0 18.5 37.0 96-97 31.666325860482353 33.0 33.0 37.0 15.0 37.0 98-99 31.48799704310793 33.0 33.0 37.0 15.0 37.0 100-101 29.84747103743993 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 0.15715146538934732 2306 2 0.15479149641497258 2306 3 0.3254914543808809 2306 4 0.21724172297479782 2306 5 0.153075521658792 2306 6 0.17163351687771922 2306 7 0.21811606266576433 2306 8 0.1633759669548951 2306 9 0.22545362077359954 2306 10-11 0.19804795748276405 2306 12-13 0.2085498967859465 2306 14-15 0.34338641173159345 2306 16-17 0.41054403794794325 2306 18-19 0.4010900700818283 2306 20-21 0.4444428415065005 2306 22-23 0.4682083793494485 2306 24-25 0.4753412202336378 2306 26-27 0.4107707401646721 2306 28-29 0.40641543558273696 2306 30-31 0.46631781521338667 2306 32-33 0.45461683551880583 2306 34-35 0.4507042907886074 2306 36-37 0.42591135893356125 2306 38-39 0.5221123134254881 2306 40-41 0.5253198734339648 2306 42-43 0.5398576772645995 2306 44-45 0.5306625066546502 2306 46-47 0.567396056394621 2306 48-49 0.593466763237295 2306 50-51 0.6180238936990108 2306 52-53 0.5687679793785918 2306 54-55 0.5163184688235987 2306 56-57 0.5814382126471642 2306 58-59 0.6437208433510335 2306 60-61 0.5604851572086815 2306 62-63 0.6672302951357167 2306 64-65 0.5806758274288768 2306 66-67 0.36200361172341644 2306 68-69 0.5736965847975029 2306 70-71 0.48501997830056354 2306 72-73 0.3524535750587887 2306 74-75 0.32842801290289714 2306 76-77 0.3193424345005731 2306 78-79 0.32264305306279795 2306 80-81 0.5228210874570536 2306 82-83 0.5044386881206222 2306 84-85 0.5783051734008424 2306 86-87 0.5455914220713183 2306 88-89 0.6660726152540235 2306 90-91 0.6134510171420615 2306 92-93 0.644273942097918 2306 94-95 0.5454099561434553 2306 96-97 0.65225142918176 2306 98-99 0.5284344167634814 2306 100-101 0.5748707002876969 2307 1 0.15354990571144356 2307 2 0.13910543215456528 2307 3 0.33781371668354865 2307 4 0.21291255778891838 2307 5 0.13772594906797053 2307 6 0.13417900549266193 2307 7 0.17998044627794485 2307 8 0.18082124724651294 2307 9 0.1840667909800473 2307 10-11 0.17688882091645297 2307 12-13 0.1698800288744522 2307 14-15 0.3200401854784971 2307 16-17 0.2735545846978056 2307 18-19 0.31942050553835344 2307 20-21 0.2483373915251974 2307 22-23 0.3462240180638787 2307 24-25 0.25891589879756793 2307 26-27 0.2958034696726628 2307 28-29 0.27931442624043257 2307 30-31 0.2225107827551298 2307 32-33 0.22110159638491922 2307 34-35 0.2318058962503997 2307 36-37 0.22767535241393233 2307 38-39 0.24131633200338598 2307 40-41 0.2839759345173647 2307 42-43 0.31301287190451177 2307 44-45 0.23358991517246608 2307 46-47 0.20115056610757165 2307 48-49 0.18506914888308046 2307 50-51 0.18770360023624022 2307 52-53 0.16943377000263382 2307 54-55 0.19400597640447614 2307 56-57 0.28621085782256017 2307 58-59 0.21570314767932075 2307 60-61 0.2226188031641314 2307 62-63 0.22352425264455889 2307 64-65 0.10577107782162898 2307 66-67 0.1027381604575126 2307 68-69 0.18930114046686697 2307 70-71 0.15917644666151176 2307 72-73 -0.05042303324956521 2307 74-75 4.2337559223426524E-4 2307 76-77 -0.30778145351495567 2307 78-79 0.18802719580660465 2307 80-81 0.24406632427387365 2307 82-83 0.24518730378837006 2307 84-85 0.2663416709891564 2307 86-87 0.3226148111353737 2307 88-89 0.3274723388520471 2307 90-91 0.25070649468658956 2307 92-93 0.09832233925045131 2307 94-95 0.17894226138742297 2307 96-97 0.1469872641113419 2307 98-99 0.003301902267239143 2307 100-101 -0.07579053848895256 2201 1 0.14692296402853344 2201 2 0.10684996600267027 2201 3 0.297477771639862 2201 4 0.2135569521182532 2201 5 0.12020090328837796 2201 6 0.18466236123912694 2201 7 0.1644281331383013 2201 8 0.16943864741338643 2201 9 0.10437094230351107 2201 10-11 0.18715890553971093 2201 12-13 0.1850932699979282 2201 14-15 0.3477850163239893 2201 16-17 0.3195645548100714 2201 18-19 0.2897976662193429 2201 20-21 0.30790714231139305 2201 22-23 0.39742556749024516 2201 24-25 0.33650259103468017 2201 26-27 0.335621894270524 2201 28-29 0.4326984514610146 2201 30-31 0.392386662847386 2201 32-33 0.3784040871229948 2201 34-35 0.336493275001466 2201 36-37 0.3746760338079369 2201 38-39 0.3745087349102434 2201 40-41 0.4630563336098561 2201 42-43 0.43003221713427564 2201 44-45 0.4420092810820506 2201 46-47 0.435523228628135 2201 48-49 0.3665974514340178 2201 50-51 0.35441615816572636 2201 52-53 0.43843497779200646 2201 54-55 0.43304919782413265 2201 56-57 0.4378767231629439 2201 58-59 0.3823802309209867 2201 60-61 0.41784848514293316 2201 62-63 0.4317787450050261 2201 64-65 0.40376698045768933 2201 66-67 0.3116347721673307 2201 68-69 0.5068195213051183 2201 70-71 0.3749388729521641 2201 72-73 0.2914020586896271 2201 74-75 0.1975951904802642 2201 76-77 0.08192476707030139 2201 78-79 0.3330216948550131 2201 80-81 0.5093023901964955 2201 82-83 0.474337512266537 2201 84-85 0.4626840181764962 2201 86-87 0.48489385807975083 2201 88-89 0.41696600130782 2201 90-91 0.46313152534631996 2201 92-93 0.37061974303330203 2201 94-95 0.4425319667980183 2201 96-97 0.5494928220624331 2201 98-99 0.5181029220182651 2201 100-101 0.32867845218392944 1101 1 0.14587515086404323 1101 2 0.12558214910538368 1101 3 0.31459541704315086 1101 4 0.20455860891842548 1101 5 0.1464175256527369 1101 6 0.13810735021478138 1101 7 0.1337174773283536 1101 8 0.14330988285898627 1101 9 0.1738484632949593 1101 10-11 0.18859154794713362 1101 12-13 0.1502229542356659 1101 14-15 0.25972461958811266 1101 16-17 0.19074748476897696 1101 18-19 0.23169680582383023 1101 20-21 0.2575391347376552 1101 22-23 0.17875497743385438 1101 24-25 0.2436342705427208 1101 26-27 0.20293463678215318 1101 28-29 0.17916939580008773 1101 30-31 0.2104721837692054 1101 32-33 0.25594527475320916 1101 34-35 0.3435473591212883 1101 36-37 0.3314841299467872 1101 38-39 0.38790429669289495 1101 40-41 0.34072574364974884 1101 42-43 0.29015746867238335 1101 44-45 0.28644189182732305 1101 46-47 0.41488416536088835 1101 48-49 0.2911792519617933 1101 50-51 0.25065536711585423 1101 52-53 0.33735097998577146 1101 54-55 0.2907036451883229 1101 56-57 0.45892482517502486 1101 58-59 0.3675934097035807 1101 60-61 0.3085546656667546 1101 62-63 0.2740559463014236 1101 64-65 0.12722616832934364 1101 66-67 0.11522234261096287 1101 68-69 0.18948717974357265 1101 70-71 0.20231561187022606 1101 72-73 0.16100079383341637 1101 74-75 0.08691263435680696 1101 76-77 -0.15305955554135053 1101 78-79 0.20134040466168557 1101 80-81 0.18844418696184562 1101 82-83 0.25751047809438177 1101 84-85 0.20096641095293677 1101 86-87 0.35050767680789363 1101 88-89 0.3342125079183518 1101 90-91 0.25927483596931467 1101 92-93 0.32156916285698145 1101 94-95 0.34307315331728105 1101 96-97 0.3616588420314315 1101 98-99 0.30238501168989274 1101 100-101 0.2098705761987958 2304 1 0.10905260925707694 2304 2 0.06157789150415027 2304 3 0.26593519572428903 2304 4 0.15144155916420488 2304 5 0.08986132952023951 2304 6 0.1331044916733859 2304 7 0.11693158425015326 2304 8 0.14419550574293538 2304 9 0.07510690878898885 2304 10-11 0.08507998827896301 2304 12-13 0.13035003040973692 2304 14-15 0.21357175165037035 2304 16-17 0.2360805907513921 2304 18-19 0.27856689168464044 2304 20-21 0.24665272801589566 2304 22-23 0.2701090397565906 2304 24-25 0.3057653708110948 2304 26-27 0.2458733269617639 2304 28-29 0.33538498299052577 2304 30-31 0.2491684308473694 2304 32-33 0.3007105094253575 2304 34-35 0.2835873926150754 2304 36-37 0.31219978019422 2304 38-39 0.25582342992934315 2304 40-41 0.36734384694693034 2304 42-43 0.36616698873890385 2304 44-45 0.3705645127330399 2304 46-47 0.37876294566540736 2304 48-49 0.31015124719501586 2304 50-51 0.3675955358353633 2304 52-53 0.3293509432183299 2304 54-55 0.3843147935798683 2304 56-57 0.3690410806768085 2304 58-59 0.2635418072865221 2304 60-61 0.3667547918987921 2304 62-63 0.33661693459652753 2304 64-65 0.3539226048103785 2304 66-67 0.23881688957906277 2304 68-69 0.29069130894551165 2304 70-71 0.3068059146917719 2304 72-73 0.1644563211148693 2304 74-75 0.0667519800719063 2304 76-77 -0.05393757195185245 2304 78-79 0.1931930873413279 2304 80-81 0.3133172665855639 2304 82-83 0.36410232219237315 2304 84-85 0.27824428095147624 2304 86-87 0.4025476769073961 2304 88-89 0.4699644257384179 2304 90-91 0.37477426525190793 2304 92-93 0.519952125267217 2304 94-95 0.3935309099616511 2304 96-97 0.317396397783952 2304 98-99 0.2337684019431734 2304 100-101 0.20614616598404467 2202 1 0.0668003045102381 2202 2 0.04291724596692603 2202 3 0.28918776175670047 2202 4 0.15843395413549644 2202 5 0.09613472671824042 2202 6 0.09730536247113974 2202 7 0.08154241904599502 2202 8 0.15547529634451962 2202 9 0.07859264329047733 2202 10-11 0.1180435331611207 2202 12-13 0.0926607428869275 2202 14-15 -0.009320621816890196 2202 16-17 -0.025199315463275695 2202 18-19 0.004104897173945687 2202 20-21 0.12764715084069422 2202 22-23 0.07155368305554788 2202 24-25 0.03960774855603688 2202 26-27 0.0793424478629916 2202 28-29 0.13644804530366628 2202 30-31 0.15023675355585908 2202 32-33 0.21061583387035654 2202 34-35 0.17793587819927836 2202 36-37 0.16155859002688544 2202 38-39 0.19554201612005073 2202 40-41 0.20065203443435564 2202 42-43 0.16901968379551846 2202 44-45 0.14462764210177426 2202 46-47 0.19963914254986292 2202 48-49 0.1948737873532238 2202 50-51 0.17888908439401519 2202 52-53 0.17364416574808672 2202 54-55 0.30558881508852664 2202 56-57 0.22008466218519374 2202 58-59 0.3121283795204235 2202 60-61 0.24781037404535766 2202 62-63 0.22629858240669165 2202 64-65 0.15078908557120485 2202 66-67 0.12054603355665705 2202 68-69 0.07096945090449935 2202 70-71 0.10901978964928816 2202 72-73 0.07584386311133073 2202 74-75 0.04660501271873585 2202 76-77 -0.1334535346504495 2202 78-79 0.2047200587178395 2202 80-81 0.20308412123910102 2202 82-83 0.29113237234532363 2202 84-85 0.29514656708461473 2202 86-87 0.3685978946068431 2202 88-89 0.26188006518282947 2202 90-91 0.25753230787614 2202 92-93 0.311142061379428 2202 94-95 0.3636087406902213 2202 96-97 0.39679356803809185 2202 98-99 0.18083680095629973 2202 100-101 0.1751643449854683 1102 1 0.09236294521175381 1102 2 0.043770329879606606 1102 3 0.26941858758043935 1102 4 0.17694319973649897 1102 5 0.09029028977052889 1102 6 0.04719022884926005 1102 7 0.10055839222827245 1102 8 0.08397540622307531 1102 9 0.037346289678481526 1102 10-11 0.04674353749746274 1102 12-13 0.12247844002340003 1102 14-15 0.07266894082233932 1102 16-17 0.10567850766237541 1102 18-19 0.12041741678855544 1102 20-21 0.10336551408377659 1102 22-23 0.1140595058842706 1102 24-25 0.09298333552436588 1102 26-27 0.07150467051149434 1102 28-29 0.1229791754799976 1102 30-31 0.1654239366444088 1102 32-33 0.10871274823048793 1102 34-35 0.08463218635314718 1102 36-37 0.0939517818189941 1102 38-39 0.13926943864831998 1102 40-41 0.08923985974287518 1102 42-43 0.039894423706719806 1102 44-45 -0.017202162990564318 1102 46-47 0.2132566056707148 1102 48-49 0.09760638262601873 1102 50-51 0.03636689002729554 1102 52-53 0.024182423698846378 1102 54-55 -0.03828472020087048 1102 56-57 0.0622626867671201 1102 58-59 0.005554940586073087 1102 60-61 -0.01793366182723588 1102 62-63 0.004666801264214371 1102 64-65 -0.04241000115054305 1102 66-67 -0.06795940120597521 1102 68-69 -0.03224705101781922 1102 70-71 -0.10329899341074622 1102 72-73 -0.20430188951271333 1102 74-75 -0.18913822570745964 1102 76-77 -0.23807966090569366 1102 78-79 -0.06505815563428285 1102 80-81 5.762441590348999E-4 1102 82-83 0.0040582252638685645 1102 84-85 -0.006687008422538554 1102 86-87 0.05529700301030971 1102 88-89 0.0527648485089216 1102 90-91 -0.06442910894725884 1102 92-93 0.007621794848581942 1102 94-95 -0.07718593486668723 1102 96-97 -0.04222533768904668 1102 98-99 -0.15205790010419307 1102 100-101 -0.28674577435219817 2305 1 0.08199637130822168 2305 2 0.05897518396282919 2305 3 0.2620657813383289 2305 4 0.11624762014030665 2305 5 0.06207940980225146 2305 6 0.029394473914500452 2305 7 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86-87 -0.06343376079303908 1213 88-89 0.010304625707142634 1213 90-91 0.10385127694757301 1213 92-93 0.028838732524370414 1213 94-95 0.21724707690244927 1213 96-97 0.25985530788554456 1213 98-99 0.11993169962632066 1213 100-101 0.40092280929073354 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 12.0 10 35.0 11 42.0 12 81.0 13 248.0 14 595.0 15 1433.0 16 2935.0 17 4671.0 18 7302.0 19 10080.0 20 13050.0 21 17190.0 22 21858.0 23 28008.0 24 35130.0 25 44286.0 26 57496.0 27 78915.0 28 120877.0 29 164593.0 30 142599.0 31 138994.0 32 180235.0 33 251140.0 34 373795.0 35 601358.0 36 1052335.0 37 1716831.0 38 796411.0 39 4434.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.155394868352175 27.175329764083244 19.235774362112362 16.433501005452218 2 29.51419054227471 27.58213735844271 28.101587684684308 14.802084414598266 3 33.67698613662116 21.90330678469697 27.503524818952478 16.916182259729386 4 29.21357796538509 22.25774334774485 27.957287134654464 20.5713915522156 5 33.017736068577804 20.515952780404064 29.261896811830155 17.204414339187974 6 30.423262702338228 21.28082787523579 30.805657638328192 17.49025178409779 7 30.799027300458786 25.081920466284902 24.997311219025967 19.12174101423034 8 28.482261119068085 30.524285870852268 22.767676881307235 18.225776128772413 9 30.916310989094715 25.27600698895563 24.63630721883575 19.171374803113906 10-11 26.11697762699742 29.88099713318287 27.800856644740772 16.201168595078933 12-13 24.057954958010694 29.563684491313236 27.17847543109987 19.199885119576205 14-15 23.294021736258795 29.815836826191916 27.68944145113381 19.20069998641548 16-17 24.752840946375905 27.88697949930211 27.66109633062785 19.69908322369413 18-19 22.001583099694884 28.23876238692845 29.909718319725805 19.849936193650862 20-21 23.696316208142157 28.039536926294677 30.105603044569335 18.15854382099383 22-23 21.60454687776708 32.5150746441392 27.579342048903943 18.301036429189782 24-25 21.192025663668698 28.36533536640968 30.09553822577272 20.347100744148896 26-27 21.333015281569132 28.13181490035017 27.870666448837 22.664503369243697 28-29 23.076550062643125 30.686643834303073 27.73556069752936 18.50124540552444 30-31 20.8675311331861 28.37571550975793 30.105509299432363 20.651244057623604 32-33 20.95115179536425 30.667588096140243 27.66213604941971 20.7191240590758 34-35 22.706026670321023 30.56577235510453 28.136179640226615 18.592021334347827 36-37 20.82225389325438 30.238666500878104 29.99836114766026 18.94071845820726 38-39 20.69474917706214 28.6298942165312 29.900719071917848 20.774637534488818 40-41 22.572332058253412 28.30950992018429 30.127012277668857 18.991145743893444 42-43 20.63097154707572 30.352808488723205 29.852513317640305 19.163706646560776 44-45 20.321228044503847 28.268649137921408 30.315452474610545 21.094670342964196 46-47 22.583872463589266 28.09388864878934 27.931150301455848 21.391088586165544 48-49 20.330915754037584 30.54577917927302 29.89223164443687 19.231073422252525 50-51 22.371275022316585 28.13082044408854 30.072802265936648 19.425102267658225 52-53 20.462507523347305 28.300305604290767 27.763192725359943 23.473994147001985 54-55 22.183102492838703 28.287706433277847 30.272934687097564 19.256256386785882 56-57 20.38636528093974 28.06156050103373 29.999975282931597 21.55209893509494 58-59 20.200495997879287 28.5937535293714 29.93616978657771 21.2695806861716 60-61 22.127924502733862 28.1866602235242 30.208971734973776 19.476443538768155 62-63 20.21362065854793 28.381052265020383 29.83720345745463 21.568123618977054 64-65 22.017720723202427 28.37821528609262 30.18859932032718 19.415464670377773 66-67 20.26688832062264 32.06140310125163 27.879264515076425 19.79244406304931 68-69 20.116410406353815 32.48209218210693 27.813596728642537 19.587900682896723 70-71 20.032239736796903 32.17535961755591 28.11333179607812 19.679068849569063 72-73 20.081348645845782 32.24226880138952 27.78983012358291 19.88655242918179 74-75 19.910041692985843 31.999635154407258 28.245263187505916 19.845059965100983 76-77 20.224504266557965 31.472034528678794 27.998452306120143 20.305008898643102 78-79 20.08604615977992 31.61065710882346 28.10985269016777 20.193444041228855 80-81 20.057822217858668 31.091037053400367 28.35459373257135 20.496546996169617 82-83 20.195496536761194 31.152249387965412 27.98750687542857 20.664747199844825 84-85 20.04273483877148 30.9526642327928 28.463062856059736 20.541538072375985 86-87 20.322641409724675 30.508803960375786 28.30992770336731 20.858626926532235 88-89 20.25396934716915 30.938946555168457 28.224840727901807 20.582243369760587 90-91 20.349278294694173 30.412465868515014 28.40626082592056 20.831995010870248 92-93 20.330643790521513 30.566832783314524 28.111661006789294 20.990862419374665 94-95 20.13189087632588 30.469571414323354 28.486042429785925 20.91249527956484 96-97 20.31769389209159 30.273130580323844 28.21504824168598 21.19412728589859 98-99 20.25051206072879 30.55482097524871 28.154371667854218 21.04029529616829 100-101 20.401207589990875 30.199484551109894 28.23091100996715 21.16839684893208 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 32.0 1 20.5 2 20.0 3 52.0 4 55.0 5 32.0 6 31.5 7 39.5 8 46.0 9 48.5 10 48.0 11 64.0 12 80.5 13 101.0 14 137.0 15 188.5 16 321.5 17 529.5 18 864.5 19 1489.5 20 2479.0 21 4040.5 22 6381.5 23 9838.5 24 14393.0 25 20293.0 26 27767.5 27 36997.5 28 47966.0 29 60262.5 30 74493.5 31 90779.5 32 109734.5 33 131583.0 34 156026.0 35 183910.0 36 216524.5 37 251715.0 38 284923.0 39 310625.5 40 327490.0 41 333510.0 42 325840.0 43 310499.0 44 291566.5 45 272362.0 46 256189.0 47 239106.5 48 218093.5 49 196827.0 50 176982.0 51 156925.0 52 135961.0 53 114911.5 54 95322.5 55 78294.5 56 64083.0 57 52060.0 58 42160.0 59 33634.0 60 26096.0 61 19894.0 62 14835.0 63 10840.5 64 7904.5 65 5788.0 66 4144.0 67 2981.0 68 2153.5 69 1505.0 70 1074.5 71 788.5 72 567.0 73 413.5 74 315.5 75 236.0 76 169.5 77 124.5 78 97.5 79 78.5 80 66.0 81 45.5 82 27.5 83 20.5 84 16.5 85 16.0 86 11.5 87 5.5 88 5.5 89 5.0 90 2.0 91 0.5 92 0.5 93 1.0 94 1.0 95 1.5 96 2.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16882646939962717 2 0.0 3 5.113371107510162E-5 4 0.02200454033265206 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.9601255912122286E-4 12-13 1.7044570358367206E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 2.556685553755081E-5 28-29 1.1078970732938685E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.001031196506681216 38-39 5.709931070053013E-4 40-41 3.153245516297933E-4 42-43 5.880376773636685E-4 44-45 2.982799812714261E-4 46-47 0.0032640352236273196 48-49 0.0037668500491991525 50-51 0.004397499152458738 52-53 0.003988429463857926 54-55 0.006238312751162397 56-57 0.009877328522673794 58-59 0.017359894909997 60-61 0.004576467141221594 62-63 0.002889054675743241 64-65 0.0105505890518293 66-67 0.023376628246500622 68-69 0.025839568663284684 70-71 0.026223071496347942 72-73 0.023078348265229198 74-75 0.025157785848949992 76-77 0.02505551842679979 78-79 0.015911106429535787 80-81 0.003323691219881605 82-83 0.0010908525029355012 84-85 0.00560766364790281 86-87 0.014675375078554162 88-89 0.016831513228887617 90-91 0.024186245338523066 92-93 0.024101022486731228 94-95 0.025839568663284684 96-97 0.02740766913625447 98-99 0.03313464477666585 100-101 0.023930576783147556 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5866971.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.10006454482536 #Duplication Level Percentage of deduplicated Percentage of total 1 97.0859254342281 85.53276297149598 2 1.9723021354850552 3.475198908762605 3 0.3596819704297879 0.9506401443142283 4 0.17769014685194692 0.6261805362654408 5 0.08754010380645917 0.3856144397804883 6 0.0456260471956162 0.2411794621715421 7 0.05478996660612445 0.3378899716085873 8 0.030841140821118336 0.2173685197581259 9 0.027433720597813998 0.21752212998349063 >10 0.13530428686305407 2.23412420510035 >50 0.015183655640256536 0.9214648411278066 >100 0.007427043503894907 1.1168332415180158 >500 1.4099070967282004E-4 0.06980779359686669 >1k 9.40591517776911E-5 0.10123082655584494 >5k 0.0 0.0 >10k+ 1.929810936900506E-5 3.572182007960617 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT 210108 3.5812005888558165 TruSeq Adapter, Index 7 (97% over 35bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0017555907469118221 0.0 0.0 2 0.001926036450495494 0.0 0.0 3 0.002164660435512635 0.0 0.0 4 0.0023691952798130414 0.0 0.0 5 0.002829398679488956 0.0 0.0 6 0.003085067234864464 0.0 0.0 7 0.0038009391899158865 0.0 3.408914071673441E-5 8 0.0044145437228171065 0.0 5.113371107510162E-5 9 0.004857702552134653 0.0 6.817828143346882E-5 10-11 0.007047929843184839 0.0 6.817828143346882E-5 12-13 0.00868420859758809 0.0 6.817828143346882E-5 14-15 0.010661378759158686 0.0 9.374513697101963E-5 16-17 0.012544803783758263 0.0 1.1931199250857043E-4 18-19 0.014820253926600285 0.0 1.1931199250857043E-4 20-21 0.017820098309672912 0.0 1.1931199250857043E-4 22-23 0.02100743296668758 0.0 1.1931199250857043E-4 24-25 0.02412658934226878 0.0 1.1931199250857043E-4 26-27 0.027424713706612834 8.522285179183603E-6 1.4487884804612126E-4 28-29 0.03130235346314137 1.7044570358367206E-5 1.7044570358367204E-4 30-31 0.04737538331108165 1.7044570358367206E-5 2.1305712947959008E-4 32-33 0.05232683100018732 1.7044570358367206E-5 2.3862398501714086E-4 34-35 0.057039654704275855 1.7044570358367206E-5 2.727131257338753E-4 36-37 0.06242573893751989 1.7044570358367206E-5 3.068022664506097E-4 38-39 0.06812714772239373 1.7044570358367206E-5 3.068022664506097E-4 40-41 0.07476600787697774 1.7044570358367206E-5 3.238468368089769E-4 42-43 0.08209517313107564 1.7044570358367206E-5 4.0054740342162934E-4 44-45 0.08936468238891926 3.408914071673441E-5 4.0906968860081295E-4 46-47 0.09736710817217266 3.408914071673441E-5 4.261142589591801E-4 48-49 0.10563372479598075 3.408914071673441E-5 4.261142589591801E-4 50-51 0.11479518136360312 3.408914071673441E-5 4.4315882931754733E-4 52-53 0.12365835794995408 3.408914071673441E-5 4.687256848550981E-4 54-55 0.13379987731318255 3.408914071673441E-5 4.942925403926489E-4 56-57 0.1456884651381437 3.408914071673441E-5 5.198593959301998E-4 58-59 0.1577986323777636 3.408914071673441E-5 5.36903966288567E-4 60-61 0.1713064543867696 3.408914071673441E-5 5.454262514677506E-4 62-63 0.18562389348779806 3.408914071673441E-5 5.454262514677506E-4 64-65 0.20156056677287137 3.408914071673441E-5 5.454262514677506E-4 66-67 0.21972155648971164 3.408914071673441E-5 5.454262514677506E-4 68-69 0.23999607293098943 3.408914071673441E-5 5.454262514677506E-4 70-71 0.26117395160126067 3.408914071673441E-5 5.624708218261178E-4 72-73 0.28447387928114864 3.408914071673441E-5 5.624708218261178E-4 74-75 0.31078217362928845 3.408914071673441E-5 5.624708218261178E-4 76-77 0.33985168837548374 5.113371107510162E-5 5.709931070053014E-4 78-79 0.3737874279589928 5.113371107510162E-5 5.965599625428522E-4 80-81 0.4115241067324178 5.113371107510162E-5 5.965599625428522E-4 82-83 0.4529338904180709 5.113371107510162E-5 5.965599625428522E-4 84-85 0.4993036440780089 5.113371107510162E-5 6.22126818080403E-4 86-87 0.5533178875436746 5.113371107510162E-5 6.562159587971374E-4 88-89 0.613774978604803 5.113371107510162E-5 7.158719550514226E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 28465 0.0 77.98596 3 CGGAAGA 29080 0.0 77.03911 4 ATCGGAA 28910 0.0 76.867714 2 GATCGGA 29065 0.0 76.73964 1 GAAGAGC 31570 0.0 71.02416 6 AGAGCAC 32615 0.0 68.530075 8 GAGCACA 32595 0.0 68.51385 9 AAGAGCA 35690 0.0 62.732086 7 GGAAGAG 38370 0.0 58.697075 5 GCCGTCT 24600 0.0 45.045967 50-51 ATGCCGT 24615 0.0 44.82479 48-49 CGTATGC 24535 0.0 44.757236 46-47 TCTCGTA 24500 0.0 44.753326 42-43 TGCCGTC 24580 0.0 44.744457 50-51 TATGCCG 24575 0.0 44.71414 48-49 TATCTCG 24465 0.0 44.458 40-41 CTCGTAT 24615 0.0 44.399334 44-45 CGTCTTC 25040 0.0 44.331814 52-53 TCGTATG 25045 0.0 44.243896 44-45 CCGTCTT 24930 0.0 44.16541 52-53 >>END_MODULE