FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n01_35008-2.341000000358b3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n01_35008-2.341000000358b3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5866971
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT2101083.5812005888558165TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG284650.077.985963
CGGAAGA290800.077.039114
ATCGGAA289100.076.8677142
GATCGGA290650.076.739641
GAAGAGC315700.071.024166
AGAGCAC326150.068.5300758
GAGCACA325950.068.513859
AAGAGCA356900.062.7320867
GGAAGAG383700.058.6970755
GCCGTCT246000.045.04596750-51
ATGCCGT246150.044.8247948-49
CGTATGC245350.044.75723646-47
TCTCGTA245000.044.75332642-43
TGCCGTC245800.044.74445750-51
TATGCCG245750.044.7141448-49
TATCTCG244650.044.45840-41
CTCGTAT246150.044.39933444-45
CGTCTTC250400.044.33181452-53
TCGTATG250450.044.24389644-45
CCGTCTT249300.044.1654152-53
Produced by FastQC (version 0.11.2)