##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n01_35008-1.341000000357b4.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2422675 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.66086412746241 33.0 33.0 33.0 33.0 33.0 2 32.71578110972376 33.0 33.0 33.0 33.0 33.0 3 32.55452712394357 33.0 33.0 33.0 33.0 33.0 4 36.604011268535814 37.0 37.0 37.0 37.0 37.0 5 36.672127710072544 37.0 37.0 37.0 37.0 37.0 6 36.5417965678434 37.0 37.0 37.0 37.0 37.0 7 36.476089033815924 37.0 37.0 37.0 37.0 37.0 8 36.41583951623722 37.0 37.0 37.0 37.0 37.0 9 36.43757086691364 37.0 37.0 37.0 37.0 37.0 10-11 36.38492843654225 37.0 37.0 37.0 37.0 37.0 12-13 36.35973459089642 37.0 37.0 37.0 37.0 37.0 14-15 38.1656361253573 40.0 37.0 40.0 37.0 40.0 16-17 38.15353730896633 40.0 37.0 40.0 37.0 40.0 18-19 38.17952511170503 40.0 37.0 40.0 37.0 40.0 20-21 38.12918447583766 40.0 37.0 40.0 37.0 40.0 22-23 38.034575004901605 40.0 37.0 40.0 35.0 40.0 24-25 37.98230344557153 40.0 37.0 40.0 33.0 40.0 26-27 37.89103325869132 40.0 37.0 40.0 33.0 40.0 28-29 37.77107247154488 40.0 37.0 40.0 33.0 40.0 30-31 37.73499623350222 40.0 37.0 40.0 33.0 40.0 32-33 37.628769645123676 40.0 37.0 40.0 33.0 40.0 34-35 37.53737954946495 40.0 37.0 40.0 33.0 40.0 36-37 37.457779520571265 40.0 37.0 40.0 33.0 40.0 38-39 37.355069086856474 40.0 37.0 40.0 33.0 40.0 40-41 37.28401622173837 40.0 37.0 40.0 33.0 40.0 42-43 37.13959590122488 40.0 37.0 40.0 33.0 40.0 44-45 37.02286872981312 37.0 37.0 40.0 33.0 40.0 46-47 36.95936248155448 37.0 37.0 40.0 33.0 40.0 48-49 36.768810302661315 37.0 37.0 40.0 33.0 40.0 50-51 36.57441464496888 37.0 37.0 40.0 33.0 40.0 52-53 36.436039089023495 37.0 37.0 40.0 33.0 40.0 54-55 36.30718296923855 37.0 37.0 40.0 33.0 40.0 56-57 36.1301571609894 37.0 37.0 40.0 33.0 40.0 58-59 35.86711610100406 37.0 37.0 40.0 30.0 40.0 60-61 35.5793781666959 37.0 33.0 40.0 27.0 40.0 62-63 35.33564427750318 37.0 33.0 40.0 27.0 40.0 64-65 35.0339442971096 37.0 33.0 37.0 27.0 40.0 66-67 34.68313640913453 37.0 33.0 37.0 27.0 40.0 68-69 34.39889688051431 37.0 33.0 37.0 27.0 40.0 70-71 34.06035497951645 37.0 33.0 37.0 27.0 40.0 72-73 33.65562116255792 37.0 33.0 37.0 27.0 37.0 74-75 33.24399682169503 37.0 33.0 37.0 27.0 37.0 76-77 30.68172144427131 33.0 30.0 35.0 24.5 37.0 78-79 32.99329315735706 35.0 33.0 37.0 27.0 37.0 80-81 33.367245297037364 37.0 33.0 37.0 27.0 37.0 82-83 33.32135201791408 37.0 33.0 37.0 27.0 37.0 84-85 33.173298936093374 37.0 33.0 37.0 27.0 37.0 86-87 33.032722300762586 37.0 33.0 37.0 27.0 37.0 88-89 32.92357992714665 37.0 33.0 37.0 27.0 37.0 90-91 32.7896961003849 33.0 33.0 37.0 27.0 37.0 92-93 32.606760915104175 33.0 33.0 37.0 27.0 37.0 94-95 32.492177448481534 33.0 33.0 37.0 27.0 37.0 96-97 32.351010556512946 33.0 33.0 37.0 24.5 37.0 98-99 32.18093636166634 33.0 33.0 37.0 22.0 37.0 100-101 30.561626301505566 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 0.17106337734897892 2306 2 0.11529369951409052 2306 3 0.3008949197585906 2306 4 0.1994082894135829 2306 5 0.15686725775177734 2306 6 0.13753567747323103 2306 7 0.1863929134319804 2306 8 0.14444940067277656 2306 9 0.17638231056035636 2306 10-11 0.2538781583523999 2306 12-13 0.1833195495303741 2306 14-15 0.35062149037754864 2306 16-17 0.3680411099719123 2306 18-19 0.4959545569381518 2306 20-21 0.39800739017873354 2306 22-23 0.4223426622794406 2306 24-25 0.381329852744436 2306 26-27 0.32556369094053395 2306 28-29 0.43826913989531135 2306 30-31 0.5417490235276645 2306 32-33 0.6166807711592668 2306 34-35 0.47724679456368335 2306 36-37 0.4474414565716742 2306 38-39 0.6567543743768738 2306 40-41 0.5358996829585436 2306 42-43 0.5147194817769289 2306 44-45 0.4903971408269001 2306 46-47 0.5584036611840304 2306 48-49 0.5702809194694325 2306 50-51 0.5846911117041671 2306 52-53 0.47938101890107987 2306 54-55 0.5994031925135488 2306 56-57 0.6350951702233871 2306 58-59 0.578174427520274 2306 60-61 0.5328517539656019 2306 62-63 0.3588742534219662 2306 64-65 0.3206922163780064 2306 66-67 0.37428641350973635 2306 68-69 0.42643687600833147 2306 70-71 0.5131103640761125 2306 72-73 0.40600518358375126 2306 74-75 0.3143613577960025 2306 76-77 0.12929845881438595 2306 78-79 0.3657299962022833 2306 80-81 0.5362944354034411 2306 82-83 0.5531136076266776 2306 84-85 0.5259066004418926 2306 86-87 0.437562582352804 2306 88-89 0.6339003276410935 2306 90-91 0.479173138165109 2306 92-93 0.6143101046917252 2306 94-95 0.5263815794197697 2306 96-97 0.6034270986534267 2306 98-99 0.43072557006674117 2306 100-101 0.4601205855156145 2307 1 0.1522555118274056 2307 2 0.148750706217065 2307 3 0.3537929088662537 2307 4 0.19792866017186128 2307 5 0.17036380153058417 2307 6 0.21500347177570234 2307 7 0.22885651837542298 2307 8 0.1922447504842566 2307 9 0.18075181202663515 2307 10-11 0.20361331231134727 2307 12-13 0.1671085629363276 2307 14-15 0.33976751572316743 2307 16-17 0.3579277354432193 2307 18-19 0.5177104220407927 2307 20-21 0.5041555363873655 2307 22-23 0.49261942181022533 2307 24-25 0.4435351575203086 2307 26-27 0.4814240805509229 2307 28-29 0.4577627511227931 2307 30-31 0.4145925509679742 2307 32-33 0.5120222039200542 2307 34-35 0.4675109860173947 2307 36-37 0.46982638011586886 2307 38-39 0.47334371245814566 2307 40-41 0.5515089728621803 2307 42-43 0.5311115272314808 2307 44-45 0.5216578760594075 2307 46-47 0.6106049075183364 2307 48-49 0.5540763478313906 2307 50-51 0.525496646894787 2307 52-53 0.48577790830199774 2307 54-55 0.4569154987556985 2307 56-57 0.48135145426191883 2307 58-59 0.5598139069924102 2307 60-61 0.549759352415542 2307 62-63 0.5394013271546783 2307 64-65 0.34064862086062675 2307 66-67 0.3958199807489464 2307 68-69 0.44018648325592835 2307 70-71 0.33482512109328866 2307 72-73 0.3438462235921307 2307 74-75 0.18440037120195285 2307 76-77 -0.008574001386701724 2307 78-79 0.28127251819641685 2307 80-81 0.3174004261868433 2307 82-83 0.5125706928801392 2307 84-85 0.43956980215953223 2307 86-87 0.4987541931613535 2307 88-89 0.40750212906547745 2307 90-91 0.38138276321748066 2307 92-93 0.29136786547933013 2307 94-95 0.2984828048072856 2307 96-97 0.408991352947524 2307 98-99 0.08757936143666001 2307 100-101 0.11228530587589702 2201 1 0.1706809110922478 2201 2 0.12887163434939453 2201 3 0.28215101140760623 2201 4 0.20668535419556378 2201 5 0.11383891085309727 2201 6 0.13565651537795986 2201 7 0.15309819243588407 2201 8 0.1845802913930612 2201 9 0.13108136396274972 2201 10-11 0.09260293063316283 2201 12-13 0.2106325901243622 2201 14-15 0.38391786983306275 2201 16-17 0.3195311719727769 2201 18-19 0.2782683629749414 2201 20-21 0.31234898178948356 2201 22-23 0.3758334642083483 2201 24-25 0.19622613524386878 2201 26-27 0.34334696856663527 2201 28-29 0.303774459722824 2201 30-31 0.33925903807108426 2201 32-33 0.3375498038947029 2201 34-35 0.3816284443480882 2201 36-37 0.39635006270031425 2201 38-39 0.27788812275030494 2201 40-41 0.2323595099684681 2201 42-43 0.32213866377372113 2201 44-45 0.33320427475386794 2201 46-47 0.3165283263028016 2201 48-49 0.3121215542766649 2201 50-51 0.3884310652673051 2201 52-53 0.5008067837060111 2201 54-55 0.46741699599886743 2201 56-57 0.45662911761194636 2201 58-59 0.3004328665272382 2201 60-61 0.44651146360762795 2201 62-63 0.34713134014663183 2201 64-65 0.34550723436591824 2201 66-67 0.23142327468392665 2201 68-69 0.2106681162808357 2201 70-71 0.1653306585164458 2201 72-73 0.08628227388049226 2201 74-75 0.23473923139588493 2201 76-77 0.06163650387345143 2201 78-79 0.3009505713049805 2201 80-81 0.32113165123279686 2201 82-83 0.13929519617565944 2201 84-85 0.3839871250253637 2201 86-87 0.3865807743768883 2201 88-89 0.5384712969468453 2201 90-91 0.3519855026179428 2201 92-93 0.22745875368516977 2201 94-95 0.2375860036792048 2201 96-97 0.29498400932625657 2201 98-99 0.21510645440833542 2201 100-101 0.12593527181163822 1101 1 0.07480255483815768 1101 2 0.12416004150543358 1101 3 0.2835491405378079 1101 4 0.19659984785514695 1101 5 0.15686184649636914 1101 6 0.18500591556847468 1101 7 0.1461494069384699 1101 8 0.15661119720957828 1101 9 0.15255655298459914 1101 10-11 0.1429690674433104 1101 12-13 0.12763367290700245 1101 14-15 0.23574594925200643 1101 16-17 0.15676540650870407 1101 18-19 0.18459092057450732 1101 20-21 0.12704012827397548 1101 22-23 0.21489001076428593 1101 24-25 0.17666535057993826 1101 26-27 0.12323279267213394 1101 28-29 0.16195691045808047 1101 30-31 0.12550714041078237 1101 32-33 0.1648585308995294 1101 34-35 0.35670025776715164 1101 36-37 0.29556104531625493 1101 38-39 0.25228738303488285 1101 40-41 0.2431710574174133 1101 42-43 0.3956948605648094 1101 44-45 0.3613603442900981 1101 46-47 0.4351176763355369 1101 48-49 0.37909314890666224 1101 50-51 0.2614524753405263 1101 52-53 0.1912397851348402 1101 54-55 0.295609902470261 1101 56-57 0.3196487416519531 1101 58-59 0.3771327608536055 1101 60-61 0.24498784703919085 1101 62-63 0.23670704562974976 1101 64-65 0.1609993051225942 1101 66-67 0.19800259316341595 1101 68-69 0.30644228726374934 1101 70-71 0.17107573204147997 1101 72-73 0.2559307788218419 1101 74-75 0.07011487511201864 1101 76-77 -0.20068666023323445 1101 78-79 0.3484004507477394 1101 80-81 0.2791245219835261 1101 82-83 0.4419068976699734 1101 84-85 0.410038093948387 1101 86-87 0.3591372576774816 1101 88-89 0.5075678059960751 1101 90-91 0.3742881273426022 1101 92-93 0.30998651161813484 1101 94-95 0.43812265084834934 1101 96-97 0.4155361354499689 1101 98-99 0.38125452028319273 1101 100-101 0.3397336807537066 2304 1 0.0896051864171028 2304 2 0.1057413497401356 2304 3 0.27714463495567543 2304 4 0.1787228437419941 2304 5 0.1559148767994003 2304 6 0.14693956997961521 2304 7 0.12758512841920577 2304 8 0.1621815300392555 2304 9 0.10967535177583443 2304 10-11 0.11598174267205508 2304 12-13 0.10415944832041646 2304 14-15 0.2601809152555319 2304 16-17 0.31563137750678294 2304 18-19 0.333262539814001 2304 20-21 0.30410313192544436 2304 22-23 0.2592552787488387 2304 24-25 0.27775764471240194 2304 26-27 0.3013975339211896 2304 28-29 0.27548178874230445 2304 30-31 0.30502347760545234 2304 32-33 0.37499989432603087 2304 34-35 0.4014911374783878 2304 36-37 0.37029164424179584 2304 38-39 0.3853628573066885 2304 40-41 0.45697569835589036 2304 42-43 0.4766837225223455 2304 44-45 0.44505567711978244 2304 46-47 0.43235480699112827 2304 48-49 0.27410411381894306 2304 50-51 0.23903342682210393 2304 52-53 0.1866531465887178 2304 54-55 0.3274527422251552 2304 56-57 0.4501089007218795 2304 58-59 0.33527591329211504 2304 60-61 0.34696874500780694 2304 62-63 0.4010744908576598 2304 64-65 0.4021086761878152 2304 66-67 0.35277246116476135 2304 68-69 0.2529529269011235 2304 70-71 0.28343198151684135 2304 72-73 0.1660296466553035 2304 74-75 -0.017947404540457512 2304 76-77 -0.16216584788537958 2304 78-79 0.24952081295708695 2304 80-81 0.14396492742296374 2304 82-83 0.28359507445163956 2304 84-85 0.1868772087809205 2304 86-87 0.2123517082776729 2304 88-89 0.300986104283659 2304 90-91 0.38958457345189146 2304 92-93 0.5006631883578336 2304 94-95 0.47013534181619576 2304 96-97 0.36794041543821265 2304 98-99 0.32500229057614405 2304 100-101 0.18073851790639495 2202 1 0.08156068491945945 2202 2 0.05341216641304669 2202 3 0.26943467268858967 2202 4 0.16033321814339274 2202 5 0.0888786609826937 2202 6 0.09299972562020997 2202 7 0.011518032215633411 2202 8 0.10270090526574904 2202 9 0.09975259880869203 2202 10-11 0.07046352420605473 2202 12-13 0.11791568510212613 2202 14-15 -0.00147226952108781 2202 16-17 0.024297049154675676 2202 18-19 -0.026883580534132534 2202 20-21 0.0877951633811449 2202 22-23 0.1522317975344194 2202 24-25 0.14323039123033965 2202 26-27 0.21464192977487073 2202 28-29 0.30025837017722523 2202 30-31 0.20834389207375636 2202 32-33 0.17340550983522718 2202 34-35 0.20481093773758374 2202 36-37 0.11940851390458107 2202 38-39 0.15567106743990422 2202 40-41 0.23526617012990414 2202 42-43 0.2742760227968901 2202 44-45 0.3298839954357007 2202 46-47 0.37538814011972477 2202 48-49 0.30988814151567823 2202 50-51 0.2950237059391938 2202 52-53 0.3120892862434914 2202 54-55 0.30635916337195823 2202 56-57 0.3689627660421948 2202 58-59 0.27684405058013795 2202 60-61 0.12913303366151752 2202 62-63 0.27485144696013464 2202 64-65 0.1746991850191293 2202 66-67 0.09343750631936132 2202 68-69 0.18240773441822 2202 70-71 0.21671505207104502 2202 72-73 0.07781386270949753 2202 74-75 0.06177345789102873 2202 76-77 -0.167280571910986 2202 78-79 0.18346835540405948 2202 80-81 0.21925294031316156 2202 82-83 0.2859802531762554 2202 84-85 0.21586542210803117 2202 86-87 0.22805355479499667 2202 88-89 0.16377045751122665 2202 90-91 0.16322128514008938 2202 92-93 0.28876053231288523 2202 94-95 0.4022890865467943 2202 96-97 0.4309405608010337 2202 98-99 0.39900558273699716 2202 100-101 0.15942372140411365 1102 1 0.16616734315061876 1102 2 0.12532808373452298 1102 3 0.31752061964010636 1102 4 0.2102860163378253 1102 5 0.08063163291150488 1102 6 0.03626137038497035 1102 7 0.06942453297522633 1102 8 0.13812665365235688 1102 9 0.07809153157189996 1102 10-11 0.10578822502991159 1102 12-13 0.10894287968859118 1102 14-15 0.13304801733895744 1102 16-17 0.16403581294336078 1102 18-19 0.10528940813346566 1102 20-21 0.13216079491353128 1102 22-23 0.24692308185985468 1102 24-25 0.22421034434586318 1102 26-27 0.22074653135592825 1102 28-29 0.22526412165773024 1102 30-31 0.27246447087102155 1102 32-33 0.20535419076291106 1102 34-35 0.16838542337673346 1102 36-37 0.08333056746078427 1102 38-39 0.2022140309993148 1102 40-41 0.13854064664601395 1102 42-43 0.1585013975355949 1102 44-45 0.10831445606979173 1102 46-47 0.19498928486267175 1102 48-49 0.07480163131572226 1102 50-51 0.09064980417448254 1102 52-53 -0.06263533683620892 1102 54-55 0.19724871908000807 1102 56-57 0.11758082410438675 1102 58-59 0.03620418845701323 1102 60-61 -0.00755109899538553 1102 62-63 0.01755301651932939 1102 64-65 0.017017983634012523 1102 66-67 -0.2748431778291547 1102 68-69 0.04444677308800493 1102 70-71 0.15821456220356112 1102 72-73 0.05327884015972728 1102 74-75 -0.020399927430396758 1102 76-77 -0.1253945226227806 1102 78-79 0.07432895538264006 1102 80-81 0.011345547082456164 1102 82-83 2.6157593743647567E-4 1102 84-85 -0.016222781803769237 1102 86-87 0.13826292851826594 1102 88-89 0.10119117632729768 1102 90-91 0.03850647924263484 1102 92-93 0.09945600530274135 1102 94-95 0.028405369583097695 1102 96-97 -0.0680278417549971 1102 98-99 -0.17058614147752849 1102 100-101 -0.25957193126992806 2305 1 0.10613885696464109 2305 2 0.02611886440719502 2305 3 0.2297166200135976 2305 4 0.1292771281142393 2305 5 0.05456165945611957 2305 6 0.07165160297275719 2305 7 0.10707572689026534 2305 8 0.04658736213136905 2305 9 0.033713032862081604 2305 10-11 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-0.05568081194004293 1213 90-91 0.05496269395466413 1213 92-93 0.03656820694983054 1213 94-95 0.14511017814837857 1213 96-97 0.18549800072432987 1213 98-99 0.1904806498218221 1213 100-101 0.46611489150992114 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 7.0 9 27.0 10 23.0 11 17.0 12 38.0 13 80.0 14 193.0 15 471.0 16 860.0 17 1517.0 18 2301.0 19 3341.0 20 4340.0 21 5668.0 22 7523.0 23 9364.0 24 11699.0 25 15059.0 26 19542.0 27 25172.0 28 35358.0 29 47724.0 30 50140.0 31 56950.0 32 77092.0 33 108375.0 34 166198.0 35 286750.0 36 549785.0 37 773887.0 38 163116.0 39 57.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 34.659259112251156 25.49482926571108 24.7216171920093 15.124294430028462 2 28.73484887572621 23.398392273004013 34.00117638560682 13.865582465662953 3 36.883516677653155 19.65568609548214 26.69324337209355 16.767553854771155 4 27.878805143932002 19.32620072696766 34.702549341154594 18.092444787945748 5 32.28918447583766 18.12657496362492 32.70682200460235 16.877418555935073 6 25.76321627952573 19.3947186477757 38.049676493958124 16.792388578740443 7 31.427162124511128 20.925794834222504 29.707988071037178 17.93905497022919 8 27.500221862197776 28.12461432094689 26.666886809002442 17.708277007852892 9 30.389713849360728 24.560413592413344 25.896911471823504 19.152961086402424 10-11 24.219977822003518 28.804235324516757 29.04906835284932 17.926718500630397 12-13 23.650345176303052 26.94038614341585 29.013136305942812 20.396132374338283 14-15 22.977163672386926 27.458573684047593 29.734013022795054 19.83024962077043 16-17 22.65555635815782 27.603062730246524 30.628705872640776 19.112675038954873 18-19 20.922018017274294 27.85538712373719 31.516381685533553 19.706213173454962 20-21 21.782492492802376 28.10847513595509 31.00593352389404 19.103098847348488 22-23 21.15973046322763 29.50529889481668 30.28165148028522 19.05331916167047 24-25 21.492792058365236 27.389889275284553 31.18653967205671 19.930778994293497 26-27 21.37411417593002 27.595319798561384 30.229496463307992 20.801069562200603 28-29 21.421113601705226 28.76018426773265 30.025069442996895 19.79363268756523 30-31 20.177138906374154 28.731133973809943 30.50495836214102 20.586768757674882 32-33 19.96877418555935 29.56164157388011 30.38352234616694 20.086061894393595 34-35 21.038521469037324 28.931429102128845 30.60945029770811 19.42059913112572 36-37 20.155003722161442 29.343840828942998 31.345903445047707 19.15525200384785 38-39 20.772059506419176 29.726462161679112 29.720188074032585 19.78129025786913 40-41 20.6626829027679 28.56467686231744 31.08877530424115 19.683864930673508 42-43 20.61771733277637 29.449677555036065 30.786884589530978 19.14572052265659 44-45 20.102399587892016 28.879610313596675 31.20816076173934 19.80982933677197 46-47 21.103493710157675 28.76477779110141 30.289484856433997 19.842243642306922 48-49 20.66053631812538 30.11443941035057 30.085400265500752 19.1396240060233 50-51 21.359024723894056 29.339209448787045 29.682274092179995 19.619491735138904 52-53 20.243182536329503 28.972355338835587 29.37440546424922 21.410056660585695 54-55 21.416966078410653 29.077682487054844 29.826590239617456 19.678761194917048 56-57 20.71630331019892 28.986547640548 29.448210738151136 20.84893831110194 58-59 20.585013352147648 28.77239712591848 30.25953553062531 20.383053991308564 60-61 20.803752888730745 29.168084759905895 30.369334429418792 19.65882792194457 62-63 20.26818183694445 29.24116964761146 29.956534481406415 20.53411403403767 64-65 21.15507664211279 28.990459159157673 29.800225228572323 20.054238970157215 66-67 20.791144747524935 30.236873998936055 28.92805675050746 20.04392450303155 68-69 21.071231498395885 30.298835265199553 28.730352157786772 19.899581078617793 70-71 20.55191161057689 31.161334313492944 28.803088452386188 19.483665623543978 72-73 20.19063461128379 31.459912915150174 28.709100145925852 19.64035232764018 74-75 20.923922860341413 30.57226356992735 28.535311362222753 19.968502207508482 76-77 21.28650460586576 29.62565767485682 29.117745965287195 19.970091753990225 78-79 21.13641875859848 29.450182212997756 29.66652596032081 19.74687306808295 80-81 20.334772005660025 30.039990027259922 29.49411046956085 20.1311274975192 82-83 19.582399007697155 29.923626941187276 29.636276016217796 20.857698034897773 84-85 19.679993428395935 29.495583981137187 30.096522498662566 20.72790009180432 86-87 19.666156345652936 29.64175217141006 30.76760963559282 19.92448184734418 88-89 19.95202459828187 30.165571074890916 30.264155757192697 19.618248569634513 90-91 20.330820225911307 29.62572576453253 29.688419269056084 20.355034740500084 92-93 19.69540143990395 30.45898899552703 29.59990190355005 20.24570766101896 94-95 19.327217605171793 30.864685076057125 29.59198798048439 20.216109338286692 96-97 19.866950725319825 31.366330379205916 29.00882935217699 19.757889543297267 98-99 20.31780610602772 30.405173985310206 28.6803401729768 20.596679735685274 100-101 20.248359374838724 30.248091012119673 28.36936618918746 21.134183423854143 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 161.0 1 104.0 2 72.0 3 149.5 4 168.0 5 106.0 6 79.0 7 78.5 8 77.0 9 79.5 10 84.0 11 76.0 12 75.0 13 86.5 14 99.5 15 101.0 16 109.0 17 132.5 18 167.0 19 238.5 20 328.5 21 468.5 22 727.5 23 1102.5 24 1643.0 25 2420.5 26 3611.5 27 5429.5 28 7269.5 29 9028.0 30 11272.0 31 13825.5 32 19399.5 33 30597.0 34 47111.5 35 70316.0 36 103523.0 37 145631.5 38 186994.0 39 204623.5 40 201928.0 41 183250.5 42 156878.5 43 146728.0 44 138152.0 45 128134.0 46 125536.5 47 111588.0 48 83525.0 49 62779.0 50 50188.5 51 40556.0 52 33514.0 53 26461.5 54 19453.5 55 13669.5 56 9539.5 57 6490.0 58 4576.5 59 3367.0 60 2519.0 61 1825.0 62 1270.5 63 899.5 64 644.5 65 475.0 66 331.0 67 234.5 68 172.5 69 122.5 70 104.0 71 85.0 72 52.0 73 30.0 74 24.0 75 24.5 76 24.5 77 16.5 78 8.0 79 4.5 80 3.0 81 1.5 82 0.5 83 0.5 84 0.5 85 0.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.167707183175622 2 0.0 3 8.255337591711641E-5 4 0.023073668568834034 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.4446840785495372E-4 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 6.191503193783731E-5 28-29 1.8574509581351193E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 9.700021670261178E-4 38-39 4.953202555026985E-4 40-41 3.3021350366846565E-4 42-43 5.572352874405358E-4 44-45 3.7149019162702386E-4 46-47 0.0032402200047468195 48-49 0.0034259651005603313 50-51 0.004107030451876542 52-53 0.0037768169482080755 54-55 0.0060676731299080565 56-57 0.009803213390157575 58-59 0.01673769696719535 60-61 0.004726180771254915 62-63 0.0026829847173062836 64-65 0.010195341925763877 66-67 0.023300690352606106 68-69 0.025653461566243926 70-71 0.025612184878285368 72-73 0.022887923473020523 74-75 0.0247866511191142 76-77 0.0247866511191142 78-79 0.01537556626456293 80-81 0.0029306448450576324 82-83 0.0011351089188603506 84-85 0.005407246122571125 86-87 0.014178542313764744 88-89 0.016180461679754815 90-91 0.02369281888821241 92-93 0.02348643544841962 94-95 0.02534388640655474 96-97 0.026417080293477252 98-99 0.0325879451432817 100-101 0.02394047901596376 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2422675.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.671146918579545 #Duplication Level Percentage of deduplicated Percentage of total 1 87.36708157002413 34.6595232881195 2 5.362132948493593 4.2544392799329085 3 2.1136307408318418 2.5155046695349834 4 0.9760627703152894 1.5488611825173444 5 0.7108627715793485 1.4100370725136495 6 0.4996543056226919 1.189311562011519 7 0.37413693566094064 1.0389708939590623 8 0.30231460738299276 0.9594533764098717 9 0.1976605690502163 0.705727932732105 >10 1.628842644598437 12.808576959339648 >50 0.2192522491780734 6.12846709309037 >100 0.208569100246995 18.05427890132256 >500 0.03137181134638448 8.23395709359668 >1k 0.008217762350652106 4.854523541434285 >5k 1.046066592119394E-4 0.210854698891401 >10k+ 1.0460665913445852E-4 1.4275124545941649 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT 34399 1.4198767890864437 TruSeq Adapter, Index 2 (97% over 37bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 5081 0.20972685151743423 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 3988 0.16461143157873012 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 3858 0.15924546214411756 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 3752 0.15487013322051038 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 3208 0.13241561497105472 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 3077 0.1270083688484836 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 2856 0.11788622080964224 No Hit TTGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC 2732 0.11276791150278101 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 8.668104471297223E-4 0.0 0.0 2 9.90640511005397E-4 0.0 0.0 3 0.0011557472628396298 0.0 0.0 4 0.0012383006387567462 0.0 0.0 5 0.001403407390590979 0.0 0.0 6 0.0015685141424252118 0.0 0.0 7 0.0017748975822180029 0.0 0.0 8 0.0019400043340522357 0.0 0.0 9 0.0021876644618035847 0.0 0.0 10-11 0.0033640500686224935 0.0 0.0 12-13 0.004416605611565727 0.0 0.0 14-15 0.005716821282260311 0.0 0.0 16-17 0.007677463960291826 0.0 2.0638343979279103E-5 18-19 0.009782575046178296 0.0 4.1276687958558206E-5 20-21 0.011846409444106205 0.0 4.1276687958558206E-5 22-23 0.014364287409578255 0.0 4.1276687958558206E-5 24-25 0.017356847286573725 0.0 8.255337591711641E-5 26-27 0.020287492131631356 0.0 8.255337591711641E-5 28-29 0.023156221944751153 0.0 8.255337591711641E-5 30-31 0.03628220871557267 0.0 8.255337591711641E-5 32-33 0.04063689929520055 0.0 8.255337591711641E-5 34-35 0.044743929747077096 0.0 8.255337591711641E-5 36-37 0.04920181204660138 0.0 8.255337591711641E-5 38-39 0.05413437625764909 0.0 8.255337591711641E-5 40-41 0.059128855500634625 0.0 8.255337591711641E-5 42-43 0.06414397308759945 0.0 1.0319171989639552E-4 44-45 0.06946866583425346 0.0 1.2383006387567462E-4 46-47 0.0758459141238507 0.0 1.2383006387567462E-4 48-49 0.08255337591711641 0.0 1.2383006387567462E-4 50-51 0.08971488127792626 0.0 1.2383006387567462E-4 52-53 0.09644298141517124 0.0 1.2383006387567462E-4 54-55 0.10327427327231263 0.0 1.2383006387567462E-4 56-57 0.11194237774360985 0.0 1.2383006387567462E-4 58-59 0.12122963253428545 0.0 1.2383006387567462E-4 60-61 0.13076454745271238 0.0 1.2383006387567462E-4 62-63 0.1422188283612123 0.0 1.2383006387567462E-4 64-65 0.15390013105348427 0.0 1.2383006387567462E-4 66-67 0.16653079756880307 0.0 1.2383006387567462E-4 68-69 0.1811221067621534 0.0 1.2383006387567462E-4 70-71 0.19775661200945235 0.0 1.2383006387567462E-4 72-73 0.2160834614630522 0.0 1.2383006387567462E-4 74-75 0.23467860938838267 0.0 1.4446840785495372E-4 76-77 0.25585355031112306 0.0 1.6510675183423282E-4 78-79 0.2786382820642472 0.0 2.0638343979279103E-4 80-81 0.30524110745353794 0.0 2.4766012775134924E-4 82-83 0.33423798074442507 0.0 2.4766012775134924E-4 84-85 0.366103583848432 0.0 2.4766012775134924E-4 86-87 0.40220004746819116 0.0 2.4766012775134924E-4 88-89 0.4403603454858782 0.0 2.4766012775134924E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAAGA 5400 0.0 68.8838 4 TCGGAAG 5425 0.0 68.39123 3 GATCGGA 5630 0.0 66.09997 1 ATCGGAA 5700 0.0 65.09165 2 GAAGAGC 5815 0.0 64.113945 6 AACGCCG 55 1.1666998E-8 60.5673 1 GGAAGAG 6505 0.0 57.532265 5 CGCGCCG 35 2.7630158E-4 54.386963 1 TTGTGTT 26705 0.0 49.04093 1 CGCGAGG 50 2.796957E-5 47.588593 1 ACGGCGA 40 5.340085E-4 47.588593 1 AACGCGG 50 2.796957E-5 47.588593 1 GACCGTC 50 2.8302811E-5 47.493324 7 CGATCGG 85 7.1086106E-9 44.789265 1 CGTATGC 4160 0.0 44.2414 46-47 GCCGTCT 4185 0.0 44.20683 50-51 TCGTATG 4175 0.0 44.139328 44-45 CTCGTAT 4175 0.0 43.968685 44-45 TGCCGTC 4200 0.0 43.935856 50-51 TATGCCG 4175 0.0 43.912716 48-49 >>END_MODULE