FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n01_35008-1.341000000357b4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n01_35008-1.341000000357b4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2422675
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT343991.4198767890864437TruSeq Adapter, Index 2 (97% over 37bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC50810.20972685151743423No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG39880.16461143157873012No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC38580.15924546214411756No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA37520.15487013322051038No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA32080.13241561497105472No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA30770.1270083688484836No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG28560.11788622080964224No Hit
TTGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC27320.11276791150278101No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA54000.068.88384
TCGGAAG54250.068.391233
GATCGGA56300.066.099971
ATCGGAA57000.065.091652
GAAGAGC58150.064.1139456
AACGCCG551.1666998E-860.56731
GGAAGAG65050.057.5322655
CGCGCCG352.7630158E-454.3869631
TTGTGTT267050.049.040931
CGCGAGG502.796957E-547.5885931
ACGGCGA405.340085E-447.5885931
AACGCGG502.796957E-547.5885931
GACCGTC502.8302811E-547.4933247
CGATCGG857.1086106E-944.7892651
CGTATGC41600.044.241446-47
GCCGTCT41850.044.2068350-51
TCGTATG41750.044.13932844-45
CTCGTAT41750.043.96868544-45
TGCCGTC42000.043.93585650-51
TATGCCG41750.043.91271648-49
Produced by FastQC (version 0.11.2)