##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n01_35005-1.3410000003578e.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2643498 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67899465027021 33.0 33.0 33.0 33.0 33.0 2 32.72821352616874 33.0 33.0 33.0 33.0 33.0 3 32.562271656721514 33.0 33.0 33.0 33.0 33.0 4 36.61870067614956 37.0 37.0 37.0 37.0 37.0 5 36.67707446724 37.0 37.0 37.0 37.0 37.0 6 36.56250203329074 37.0 37.0 37.0 37.0 37.0 7 36.510991118586055 37.0 37.0 37.0 37.0 37.0 8 36.44500506525823 37.0 37.0 37.0 37.0 37.0 9 36.45400450463742 37.0 37.0 37.0 37.0 37.0 10-11 36.408922382388795 37.0 37.0 37.0 37.0 37.0 12-13 36.38858001784 37.0 37.0 37.0 37.0 37.0 14-15 38.22102135125505 40.0 37.0 40.0 37.0 40.0 16-17 38.22068183898759 40.0 37.0 40.0 37.0 40.0 18-19 38.237470389612554 40.0 37.0 40.0 37.0 40.0 20-21 38.16703001099301 40.0 37.0 40.0 37.0 40.0 22-23 38.07224745394171 40.0 37.0 40.0 37.0 40.0 24-25 38.02188312607008 40.0 37.0 40.0 35.0 40.0 26-27 37.9494018909793 40.0 37.0 40.0 35.0 40.0 28-29 37.82820225322659 40.0 37.0 40.0 33.0 40.0 30-31 37.79381996884431 40.0 37.0 40.0 33.0 40.0 32-33 37.68297214524089 40.0 37.0 40.0 33.0 40.0 34-35 37.593567122048135 40.0 37.0 40.0 33.0 40.0 36-37 37.51260545686057 40.0 37.0 40.0 33.0 40.0 38-39 37.42013536609447 40.0 37.0 40.0 33.0 40.0 40-41 37.34803865938238 40.0 37.0 40.0 33.0 40.0 42-43 37.20287758871011 40.0 37.0 40.0 33.0 40.0 44-45 37.076423927689746 40.0 37.0 40.0 33.0 40.0 46-47 37.0011199176243 38.5 37.0 40.0 33.0 40.0 48-49 36.82308516972587 37.0 37.0 40.0 33.0 40.0 50-51 36.639113969445035 37.0 37.0 40.0 33.0 40.0 52-53 36.501095139848786 37.0 37.0 40.0 33.0 40.0 54-55 36.366290233622266 37.0 37.0 40.0 33.0 40.0 56-57 36.177134993103834 37.0 37.0 40.0 33.0 40.0 58-59 35.92967443137842 37.0 37.0 40.0 33.0 40.0 60-61 35.649133837059836 37.0 35.0 40.0 27.0 40.0 62-63 35.381535185576084 37.0 33.0 40.0 27.0 40.0 64-65 35.08456238665586 37.0 33.0 37.0 27.0 40.0 66-67 34.75791262183667 37.0 33.0 37.0 27.0 40.0 68-69 34.46941476785683 37.0 33.0 37.0 27.0 40.0 70-71 34.15589514348034 37.0 33.0 37.0 27.0 40.0 72-73 33.80901404124384 37.0 33.0 37.0 27.0 37.0 74-75 33.447972724019465 37.0 33.0 37.0 27.0 37.0 76-77 30.837053593382706 33.0 30.0 35.0 24.5 37.0 78-79 33.18395455566828 35.0 33.0 37.0 27.0 37.0 80-81 33.56256331572787 37.0 33.0 37.0 27.0 37.0 82-83 33.51377228202934 37.0 33.0 37.0 27.0 37.0 84-85 33.36729515210528 37.0 33.0 37.0 27.0 37.0 86-87 33.222065800692874 37.0 33.0 37.0 27.0 37.0 88-89 33.10986408917276 37.0 33.0 37.0 27.0 37.0 90-91 32.98706202917498 37.0 33.0 37.0 27.0 37.0 92-93 32.83275001532061 33.0 33.0 37.0 27.0 37.0 94-95 32.7129776530945 33.0 33.0 37.0 27.0 37.0 96-97 32.57121719025322 33.0 33.0 37.0 27.0 37.0 98-99 32.40150588349225 33.0 33.0 37.0 24.5 37.0 100-101 30.739720627744 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 0.1632660319799868 2306 2 0.14855186096646378 2306 3 0.34297585600459257 2306 4 0.2219169981010083 2306 5 0.1450846560490362 2306 6 0.18542745799664573 2306 7 0.12762655031446002 2306 8 0.17222392468291758 2306 9 0.15544078821838525 2306 10-11 0.18572757588501076 2306 12-13 0.2270139604630117 2306 14-15 0.407660739769625 2306 16-17 0.5207756982518745 2306 18-19 0.4905775519378537 2306 20-21 0.44775792078532817 2306 22-23 0.4200921508481201 2306 24-25 0.467604310065191 2306 26-27 0.4886461052706679 2306 28-29 0.5241022771491615 2306 30-31 0.4648519861989726 2306 32-33 0.5661394850964498 2306 34-35 0.58323998270631 2306 36-37 0.5858677955999241 2306 38-39 0.6251738875030739 2306 40-41 0.6489052911497524 2306 42-43 0.6671690638010972 2306 44-45 0.6623541108335615 2306 46-47 0.6004576177998544 2306 48-49 0.6194654900592482 2306 50-51 0.45984161781093746 2306 52-53 0.5372998327263332 2306 54-55 0.6749864090566149 2306 56-57 0.6716351597675114 2306 58-59 0.627014008645844 2306 60-61 0.6748722139245089 2306 62-63 0.7689273928174103 2306 64-65 0.3602377288897145 2306 66-67 0.46012554376812886 2306 68-69 0.5339682085604736 2306 70-71 0.6182034992594723 2306 72-73 0.5628028374413176 2306 74-75 0.39687255047537917 2306 76-77 0.1726315375534746 2306 78-79 0.2909049435973614 2306 80-81 0.45853961713885383 2306 82-83 0.41019558475476003 2306 84-85 0.495823808251707 2306 86-87 0.48687693836946266 2306 88-89 0.44561625543884276 2306 90-91 0.428555104154988 2306 92-93 0.5635314960910378 2306 94-95 0.5014267530766361 2306 96-97 0.4786927191354735 2306 98-99 0.34052812196817683 2306 100-101 0.5675917371378709 2307 1 0.1765410397506315 2307 2 0.11033856127546215 2307 3 0.32090447575483694 2307 4 0.21883067992501282 2307 5 0.1522802752324779 2307 6 0.1146332064470883 2307 7 0.14402992917464275 2307 8 0.1319637451352591 2307 9 0.1701163408485229 2307 10-11 0.20958986173512528 2307 12-13 0.23642558535102154 2307 14-15 0.41443720110837745 2307 16-17 0.3574540665988266 2307 18-19 0.37234121456378233 2307 20-21 0.2849084702157896 2307 22-23 0.3977721098922373 2307 24-25 0.35527777833352303 2307 26-27 0.38826580066130845 2307 28-29 0.44353940804342074 2307 30-31 0.40066049917138713 2307 32-33 0.4460546479143588 2307 34-35 0.5104898135804561 2307 36-37 0.387497345999428 2307 38-39 0.26182757261816647 2307 40-41 0.3113369551652525 2307 42-43 0.4796267823391176 2307 44-45 0.47331163739089277 2307 46-47 0.41213187227483417 2307 48-49 0.3410326924434699 2307 50-51 0.3855528605659515 2307 52-53 0.3620945962244093 2307 54-55 0.41247521018290456 2307 56-57 0.43374731305951997 2307 58-59 0.4400851512052171 2307 60-61 0.3443038596561365 2307 62-63 0.41861473854721254 2307 64-65 0.24548446978891292 2307 66-67 0.2700628300753394 2307 68-69 0.18385439143579418 2307 70-71 0.06858608935326771 2307 72-73 -0.09242315125924705 2307 74-75 -0.06690023580439686 2307 76-77 -0.3966145268423382 2307 78-79 -0.04944117016491134 2307 80-81 0.1036665072344718 2307 82-83 0.2496540163639338 2307 84-85 0.36894742553362647 2307 86-87 0.27302096045638535 2307 88-89 0.25657606748060147 2307 90-91 0.09197775421989007 2307 92-93 0.0788963508256515 2307 94-95 0.06854914533889911 2307 96-97 0.2679413432738471 2307 98-99 -0.06628856389656335 2307 100-101 -0.08714927176637488 2201 1 0.12650080691378918 2201 2 0.14898434110767766 2201 3 0.31997056032939497 2201 4 0.2290352681804464 2201 5 0.13758244951770138 2201 6 0.16179550742295135 2201 7 0.16934323169133592 2201 8 0.19057785230868518 2201 9 0.13811069113100416 2201 10-11 0.14504355117185952 2201 12-13 0.21850998870661442 2201 14-15 0.3036625049946977 2201 16-17 0.33184586594826015 2201 18-19 0.3149045598813629 2201 20-21 0.3171974618268152 2201 22-23 0.3781526098066408 2201 24-25 0.27394367458416724 2201 26-27 0.3092065642291928 2201 28-29 0.39181410415709905 2201 30-31 0.42634360137991223 2201 32-33 0.3090057692976913 2201 34-35 0.3292130630240493 2201 36-37 0.24139736311978766 2201 38-39 0.25536806226035225 2201 40-41 0.21429364066430878 2201 42-43 0.3274845977816838 2201 44-45 0.3027115689094657 2201 46-47 0.17823784727911374 2201 48-49 0.3336645191854615 2201 50-51 0.3081050776520655 2201 52-53 0.41778968268220495 2201 54-55 0.4255380418898014 2201 56-57 0.312414065327971 2201 58-59 0.3420625523351646 2201 60-61 0.2934247293702228 2201 62-63 0.27911936104336377 2201 64-65 0.2973184879364936 2201 66-67 0.27344196036124657 2201 68-69 0.4574920390988595 2201 70-71 0.32439502617915394 2201 72-73 0.2934846556231392 2201 74-75 0.128282523997008 2201 76-77 -0.08902336094608287 2201 78-79 0.19970238402101614 2201 80-81 0.3067280549146645 2201 82-83 0.31256319287480494 2201 84-85 0.3994888567953936 2201 86-87 0.45615098956099587 2201 88-89 0.431589335756577 2201 90-91 0.49183180318411246 2201 92-93 0.4403276125958939 2201 94-95 0.46277273718961 2201 96-97 0.49940984181861836 2201 98-99 0.34841867801407034 2201 100-101 0.38786314049179893 1101 1 0.12557847681669898 1101 2 0.11898434110768363 1101 3 0.30489112520141504 1101 4 0.1656063184893597 1101 5 0.10408730388663656 1101 6 0.19032595755533777 1101 7 0.14276335525708816 1101 8 0.19146487790445121 1101 9 0.21316364788299325 1101 10-11 0.17445220077468093 1101 12-13 0.1866344370737778 1101 14-15 0.2374268474483614 1101 16-17 0.2515347450303409 1101 18-19 0.16968170021674922 1101 20-21 0.2220518307588506 1101 22-23 0.12805772895932677 1101 24-25 0.21089645481364272 1101 26-27 0.14665581400853256 1101 28-29 0.24543943513680233 1101 30-31 0.3092253313004747 1101 32-33 0.23985969692346032 1101 34-35 0.37589223336826905 1101 36-37 0.40296841342869527 1101 38-39 0.2827202246610554 1101 40-41 0.36922744472432356 1101 42-43 0.3258186666254588 1101 44-45 0.2981219837373601 1101 46-47 0.35544437861185685 1101 48-49 0.3901406886470724 1101 50-51 0.4413508852425352 1101 52-53 0.3906758256654399 1101 54-55 0.48873309925961905 1101 56-57 0.5289387784788957 1101 58-59 0.44071656822218586 1101 60-61 0.4775862474637833 1101 62-63 0.4024004731351525 1101 64-65 0.4171000413345496 1101 66-67 0.27126852699849024 1101 68-69 0.23165135066108888 1101 70-71 0.18375954515532555 1101 72-73 0.32491669445808924 1101 74-75 0.27165189734211026 1101 76-77 0.0078919082507376 1101 78-79 0.24761279884891252 1101 80-81 0.1545237301132545 1101 82-83 0.33329796780860477 1101 84-85 0.44077085149972106 1101 86-87 0.5114356321029163 1101 88-89 0.48462772940177246 1101 90-91 0.4201261544638939 1101 92-93 0.35863299653234293 1101 94-95 0.36969683251175667 1101 96-97 0.5465435576173832 1101 98-99 0.38143721287726606 1101 100-101 0.36205334349268625 2304 1 0.09681353887456368 2304 2 0.05337118331575397 2304 3 0.2718686343224377 2304 4 0.15290193224264215 2304 5 0.10593616284348428 2304 6 0.09735143194535567 2304 7 0.12982551477138315 2304 8 0.13397141405420143 2304 9 0.04449874441850454 2304 10-11 0.06517598856687101 2304 12-13 0.09203872254261114 2304 14-15 0.26849607924167884 2304 16-17 0.2321395527084249 2304 18-19 0.24993259050994965 2304 20-21 0.24369470759780398 2304 22-23 0.21508789519164395 2304 24-25 0.19915395313243067 2304 26-27 0.1462761426954131 2304 28-29 0.22334387327067162 2304 30-31 0.18547533556751006 2304 32-33 0.26123327642742566 2304 34-35 0.22605595476917273 2304 36-37 0.19357105534933794 2304 38-39 0.2504518611430342 2304 40-41 0.40241568621652135 2304 42-43 0.25352134036869955 2304 44-45 0.3039695288266415 2304 46-47 0.23035235288524092 2304 48-49 0.19142665928000469 2304 50-51 0.19157608988005848 2304 52-53 0.17326962173756044 2304 54-55 0.28807662062950357 2304 56-57 0.2754430335664324 2304 58-59 0.16467750721268715 2304 60-61 0.23350149617883886 2304 62-63 0.36669202580876714 2304 64-65 0.31709277789234136 2304 66-67 0.28874013983540436 2304 68-69 0.4430998251675433 2304 70-71 0.2328096841468792 2304 72-73 0.2508238945964578 2304 74-75 0.19098274277264693 2304 76-77 0.01631105324307569 2304 78-79 0.17040373574192103 2304 80-81 0.288834258766677 2304 82-83 0.3243392799162521 2304 84-85 0.17631631704464468 2304 86-87 0.3344077188652079 2304 88-89 0.5881636413392641 2304 90-91 0.360393255698078 2304 92-93 0.42114411452971723 2304 94-95 0.36105788779319425 2304 96-97 0.3112675790534496 2304 98-99 0.22320634844420084 2304 100-101 0.2923858384642166 2202 1 0.12461448994533697 2202 2 0.09600506166022882 2202 3 0.30719008618341803 2202 4 0.16497235983622716 2202 5 0.07948090014305365 2202 6 0.11139883017822427 2202 7 0.1378825260725165 2202 8 0.09116493463088204 2202 9 0.11298748784558654 2202 10-11 0.1317767973917654 2202 12-13 0.07535430455504155 2202 14-15 0.1382238266299396 2202 16-17 0.024531604234610427 2202 18-19 0.05099052217368438 2202 20-21 0.1067345161482649 2202 22-23 0.17918537068026552 2202 24-25 0.18004923739916734 2202 26-27 0.21375575220753262 2202 28-29 0.20661109874362893 2202 30-31 0.17330925379730644 2202 32-33 0.2048742591240753 2202 34-35 0.1522273434048529 2202 36-37 0.03482465451422456 2202 38-39 0.10436190209608043 2202 40-41 0.11195277824131011 2202 42-43 0.17653490578988595 2202 44-45 0.3478114382393187 2202 46-47 0.26591285194590597 2202 48-49 0.2915957306844348 2202 50-51 0.33830994978198703 2202 52-53 0.20364370545614463 2202 54-55 0.2971266175851568 2202 56-57 0.16667064550343724 2202 58-59 0.17045064268423715 2202 60-61 0.095019838573144 2202 62-63 0.2783535406214881 2202 64-65 0.1606678439193132 2202 66-67 0.04814561912547788 2202 68-69 0.09798158548676383 2202 70-71 0.14910848520472797 2202 72-73 0.16429154380681865 2202 74-75 0.12018797480902776 2202 76-77 -0.12433323587608314 2202 78-79 0.23973271816326047 2202 80-81 0.4130510901956086 2202 82-83 0.39599281766479777 2202 84-85 0.31628043790423277 2202 86-87 0.4809106498185969 2202 88-89 0.41722168595258324 2202 90-91 0.348387244662554 2202 92-93 0.3597526639305997 2202 94-95 0.4654057407363723 2202 96-97 0.5333322797502973 2202 98-99 0.38288573046880003 2202 100-101 0.3294101817462334 1102 1 0.13072584417161437 1102 2 0.07706928894672416 1102 3 0.2681151206870709 1102 4 0.21785046937269925 1102 5 0.1184691857323088 1102 6 0.044396103548137944 1102 7 0.1254460632859704 1102 8 0.10355847824013154 1102 9 0.13638937965559705 1102 10-11 0.08165308917484282 1102 12-13 0.06185201553224573 1102 14-15 0.06403508323612783 1102 16-17 0.17026240842217533 1102 18-19 0.0771623840244331 1102 20-21 0.1771825586971616 1102 22-23 0.24128226703465572 1102 24-25 0.15372757920412994 1102 26-27 0.145491959162122 1102 28-29 0.2244743128009148 1102 30-31 0.2546558219462298 1102 32-33 0.16688952488636488 1102 34-35 0.1669738677930468 1102 36-37 0.12399050768014064 1102 38-39 0.26876970160461866 1102 40-41 0.20187560787742598 1102 42-43 0.22523049942102347 1102 44-45 0.07897833493032635 1102 46-47 0.23082726605705517 1102 48-49 0.20804976508710382 1102 50-51 0.17514680641510694 1102 52-53 0.09060637418741635 1102 54-55 0.16614907020574776 1102 56-57 0.04435519796582099 1102 58-59 0.02075107692532896 1102 60-61 0.05877122713476268 1102 62-63 0.030784785782550728 1102 64-65 0.050634434285228735 1102 66-67 -0.005470111173778491 1102 68-69 -0.02219126939591831 1102 70-71 -0.02194252970758015 1102 72-73 -0.061582408460317595 1102 74-75 -0.048915687627435034 1102 76-77 0.033979619679843864 1102 78-79 0.04735886688241919 1102 80-81 -0.09091725059948885 1102 82-83 0.09748867722651511 1102 84-85 0.025226561713431295 1102 86-87 0.26317408941196163 1102 88-89 0.3600878454436085 1102 90-91 0.06093016383984917 1102 92-93 -7.789447811532568E-4 1102 94-95 0.020675121690352682 1102 96-97 0.0043353261703273915 1102 98-99 -0.0220992057415188 1102 100-101 -0.0900578729210153 2305 1 0.10171019843446771 2305 2 0.08621076728507404 2305 3 0.24932528200452708 2305 4 0.13687863917660792 2305 5 0.05642941403171875 2305 6 -0.00782458172393774 2305 7 0.03673361212048576 2305 8 -0.021029037570720277 2305 9 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94-95 -1.3690106485597653E-4 1213 96-97 0.13555565960236748 1213 98-99 0.10004528895102283 1213 100-101 0.33148992558410484 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 6.0 9 7.0 10 22.0 11 28.0 12 44.0 13 122.0 14 338.0 15 710.0 16 1313.0 17 2030.0 18 2972.0 19 3984.0 20 5140.0 21 6275.0 22 8068.0 23 10039.0 24 12440.0 25 15510.0 26 19485.0 27 24761.0 28 33648.0 29 45323.0 30 51093.0 31 61477.0 32 81671.0 33 114779.0 34 176713.0 35 303125.0 36 586752.0 37 871703.0 38 203442.0 39 477.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.4128561067606 26.058932348525556 21.85673805411542 14.671473490598425 2 32.59559871049647 22.630885289113138 31.248784754140157 13.524731246250232 3 37.714914102450614 19.10298400074447 26.137035095165572 17.045066801639344 4 31.8098043095858 18.66230983476856 31.62572614390486 17.90215971174078 5 31.304506377534615 17.183519715165286 33.85404490565153 17.657929001648572 6 27.124741535647086 17.886187165641886 37.18561542320062 17.803455875510405 7 33.826013864962256 18.857513794222655 27.672500603367205 19.64397173744788 8 31.019391730199906 26.708777536430894 23.481424990675233 18.79040574269396 9 29.090243306406887 25.530490282194272 25.656270592979453 19.72299581841938 10-11 24.07895152460067 28.19009905634415 30.594850643778287 17.1360987752769 12-13 23.78052182761663 27.071294752501185 30.01529224067736 19.132891179204822 14-15 22.757876117175048 27.795954451261167 30.709366907030006 18.73680252453378 16-17 22.469564947656476 27.972822373990823 31.315495604687428 18.242117073665273 18-19 21.578283773999452 27.865559194673118 31.725785304168948 18.830371727158486 20-21 22.131338854805264 28.100134745704363 31.226219955528624 18.54230644396175 22-23 21.46491126530075 28.677419086377213 30.943356113755332 18.9143135345667 24-25 21.71004101383848 27.39205779614738 31.41305573145885 19.484845458555295 26-27 21.54527826387612 27.681768626267168 30.954004882924064 19.818948226932648 28-29 21.435410318536636 28.682596335533074 30.48227819610024 19.399715149830055 30-31 20.547358083872204 28.96573025589579 30.74570133966434 19.741210320567674 32-33 20.314976595405028 29.592929519901283 30.962856790510152 19.12923709418354 34-35 21.11766303587141 28.90978166051194 31.038306062648807 18.934249240967837 36-37 20.48813851182782 29.107941793195387 31.805985388150653 18.59793430682614 38-39 21.075437420145583 29.1969021924677 30.44502848042308 19.282631906963633 40-41 21.018111841857635 28.511648325708634 30.903636346097574 19.56660348633616 42-43 20.897047399766254 29.038312514528652 30.83802111872459 19.226618966980503 44-45 20.517070432296105 28.521548604893006 31.371059232703445 19.590321730107448 46-47 21.154457615330475 28.39749498234197 30.72174265519085 19.726304747136705 48-49 20.765235490466193 29.905820458699438 30.085304636040927 19.24363941479344 50-51 21.47742103889324 29.10174056798278 29.63323610969248 19.7876022834315 52-53 20.82566879391722 28.84484034082666 29.511709650822343 20.81778121443378 54-55 21.80409186707277 28.915603397367196 29.719888580398823 19.560416155161214 56-57 21.253874721484443 29.121145920682252 29.53151124872766 20.09346810910565 58-59 21.034284647341995 29.282232530546466 30.012067436139915 19.67141538597162 60-61 21.124399633197445 29.484422240582525 29.96288091325362 19.42829721296641 62-63 20.635228075962715 29.305653623693622 29.744477673596997 20.314640626746666 64-65 21.13460546088204 28.97397356876193 29.713256780703652 20.17816418965238 66-67 21.186161711556274 29.20762502799925 29.442175496267776 20.1640377641767 68-69 21.28539585350562 29.675344011920423 29.286333474468368 19.75292666010559 70-71 20.589615819095737 30.703785846866825 29.003504352203763 19.70309398183367 72-73 20.362790402019577 30.558913569820778 28.986057524770793 20.092238503388852 74-75 20.84833757935996 29.800315037139647 29.047892380346617 20.30345500315378 76-77 20.819357023533172 29.246594131747177 29.32192904291366 20.612119801805996 78-79 20.63705887727647 29.303966801098486 29.532145876460575 20.526828445164476 80-81 20.077396080941494 29.74023569128652 29.48849698429929 20.693871243472696 82-83 19.557842783627514 29.48231142268793 29.653450018280886 21.30639577540367 84-85 19.753589661623202 29.322735690060547 29.96116677479945 20.9625078735168 86-87 20.013105683871537 29.30429400316595 30.541502341165465 20.141097971797052 88-89 20.176507098856575 29.866350316951916 29.98787582529611 19.969266758895397 90-91 20.47739865182518 29.61869820214979 29.40435085388 20.499552292145033 92-93 19.873199822089273 30.114417939243598 29.60272594653181 20.40965629213532 94-95 19.553781715943877 30.42053582594123 29.65083775894587 20.374844699169024 96-97 19.885887109666694 30.956725605569652 29.18766931478346 19.969717969980188 98-99 20.43688258652776 30.212572246789055 28.805268746324753 20.545276420358437 100-101 20.383195038801926 30.074576886840642 28.24678101032574 21.29544706403169 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 394.0 1 248.5 2 106.0 3 174.5 4 204.0 5 146.5 6 128.0 7 130.0 8 140.0 9 149.5 10 142.5 11 135.0 12 143.5 13 154.5 14 156.5 15 163.5 16 177.0 17 187.5 18 206.0 19 242.5 20 320.0 21 470.0 22 695.5 23 1050.5 24 1606.0 25 2474.0 26 4208.0 27 7036.5 28 9990.5 29 13003.5 30 16628.0 31 19839.5 32 25420.0 33 36692.5 34 53717.0 35 78646.5 36 114104.5 37 153864.5 38 187347.0 39 204196.0 40 203917.5 41 189327.0 42 170642.5 43 161796.0 44 149248.5 45 133739.0 46 125011.5 47 111334.5 48 92969.5 49 77566.5 50 63857.5 51 52255.0 52 42517.0 53 34003.5 54 27696.5 55 21838.5 56 15560.5 57 10203.0 58 6864.5 59 4938.5 60 3683.0 61 2748.0 62 1953.0 63 1404.0 64 1040.5 65 758.5 66 537.5 67 395.5 68 305.0 69 231.0 70 155.0 71 95.5 72 73.0 73 53.0 74 40.5 75 27.5 76 17.5 77 13.5 78 11.0 79 6.0 80 3.5 81 4.0 82 2.5 83 2.0 84 1.0 85 0.0 86 2.0 87 2.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.169169789422954 2 0.0 3 0.0 4 0.02265937027378118 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 9.457166224449573E-5 12-13 1.8914332448899148E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 1.1348599469339487E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0010213739522405539 38-39 5.863443059158736E-4 40-41 2.458863218356889E-4 42-43 5.485156410180753E-4 44-45 3.404579840801847E-4 46-47 0.0031586935189661576 48-49 0.003385665508352948 50-51 0.0040854958089622155 52-53 0.0038396094871265274 54-55 0.005939100388954332 56-57 0.009211279902613884 58-59 0.01643655489809336 60-61 0.004312467798349005 62-63 0.0026290922103969815 64-65 0.009911110203223153 66-67 0.02156233899174503 68-69 0.02417251686969311 70-71 0.024267088531937604 72-73 0.021448852997051633 74-75 0.024021202210101917 76-77 0.023945544880306324 78-79 0.015074722961772622 80-81 0.0031208648540683595 82-83 0.0010024596197916549 84-85 0.005409499080385156 86-87 0.013996606012185369 88-89 0.016323068903399962 90-91 0.022375655287047692 92-93 0.02267828460623008 94-95 0.024342745861733205 96-97 0.025591091803360548 98-99 0.031019505216194603 100-101 0.0222432549599054 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2643498.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.72842383964774 #Duplication Level Percentage of deduplicated Percentage of total 1 87.21924800484354 40.756179877456795 2 5.734213704967337 5.35901536785661 3 1.9623626430034173 2.7509433992806516 4 1.09229759125702 2.041653792131374 5 0.6840089589264083 1.5981330271414704 6 0.4791496804500545 1.3433945610421154 7 0.33940902256485705 1.1102034062987842 8 0.2614328072558867 0.9773074418433607 9 0.1979995459227669 0.8326986031743162 >10 1.6732957862814102 15.464314034113247 >50 0.19925569144108735 6.369547489013505 >100 0.1404338240018755 14.268789403212438 >500 0.014412563385098484 4.359174127977323 >1k 0.002235174593980668 1.5219784656882502 >5k 1.6333407136778693E-4 0.39146290907659825 >10k+ 8.166703391749917E-5 0.855204094693191 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT 22410 0.8477403803596598 TruSeq Adapter, Index 2 (97% over 37bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 5204 0.19686037212814234 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 5054 0.19118607239347257 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 6.430873032625711E-4 0.0 0.0 2 7.187446330581676E-4 0.0 0.0 3 7.187446330581676E-4 0.0 0.0 4 7.187446330581676E-4 0.0 0.0 5 9.457166224449574E-4 0.0 0.0 6 0.0011348599469339488 0.0 0.0 7 0.0013618319363207386 0.0 0.0 8 0.0014374892661163353 0.0 0.0 9 0.00177794725019652 0.0 0.0 10-11 0.002421034553459091 0.0 0.0 12-13 0.0033856655083529474 0.0 0.0 14-15 0.004860983439367081 0.0 0.0 16-17 0.006374130035279013 0.0 0.0 18-19 0.008303391945066727 0.0 0.0 20-21 0.010119167860161045 0.0 0.0 22-23 0.012351059089131144 0.0 0.0 24-25 0.015434095278301705 0.0 0.0 26-27 0.018441474137676668 0.0 0.0 28-29 0.021637996321540627 0.0 0.0 30-31 0.032646137806799924 0.0 0.0 32-33 0.03673163361576215 0.0 0.0 34-35 0.04077930075982657 0.0 0.0 36-37 0.044883710901237675 0.0 0.0 38-39 0.04982035167040036 0.0 0.0 40-41 0.055721623394456896 0.0 0.0 42-43 0.061168951139739844 0.0 0.0 44-45 0.06691890820420518 0.0 0.0 46-47 0.07257429360642603 0.0 0.0 48-49 0.0799508832614967 0.0 0.0 50-51 0.08689244327024269 0.0 0.0 52-53 0.09400423227102878 0.0 0.0 54-55 0.10221305255385099 0.0 0.0 56-57 0.11131084646177149 0.0 0.0 58-59 0.12194070129805282 0.0 0.0 60-61 0.13192746883107154 0.0 0.0 62-63 0.1437678409440824 0.0 0.0 64-65 0.15695113066096514 0.0 1.8914332448899148E-5 66-67 0.17096665100559938 0.0 3.7828664897798296E-5 68-69 0.18753560623083504 0.0 3.7828664897798296E-5 70-71 0.2066390820042232 0.0 3.7828664897798296E-5 72-73 0.22914713761841318 0.0 3.7828664897798296E-5 74-75 0.2506149049479137 0.0 3.7828664897798296E-5 76-77 0.2765275404029055 0.0 3.7828664897798296E-5 78-79 0.30552321204706795 0.0 3.7828664897798296E-5 80-81 0.33873677982733486 0.0 5.674299734669744E-5 82-83 0.375090126794119 0.0 7.565732979559659E-5 84-85 0.41812023311536456 0.0 7.565732979559659E-5 86-87 0.46716509715536003 0.0 7.565732979559659E-5 88-89 0.5222247189141054 0.0 7.565732979559659E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAGTCG 20 0.0020606087 71.36971 1 CGCGTGG 30 1.2907709E-4 63.439747 1 TCGTGCG 30 1.3010895E-4 63.337734 2 CGAGGCG 100 0.0 57.09577 1 TCGGAAG 4280 0.0 52.719784 3 CGGAAGA 4345 0.0 52.368427 4 GAAGAGC 4580 0.0 49.570175 6 GATCGGA 4595 0.0 49.288334 1 ATCGGAA 4620 0.0 48.839973 2 TCGCCGG 55 4.963036E-5 43.18482 2 GTATGCG 45 9.5481344E-4 42.293163 1 ACCGCGA 45 9.624058E-4 42.22516 3 CGTATGC 2730 0.0 41.75561 46-47 TGCCGTC 2750 0.0 41.53908 50-51 TATGCCG 2750 0.0 41.45233 48-49 ATGCCGT 2735 0.0 41.332344 48-49 GCCGTCT 2765 0.0 41.141953 50-51 TCGTATG 2825 0.0 40.35106 44-45 CTCGTAT 2795 0.0 40.10443 44-45 CGCGGAG 95 1.9015715E-8 40.067207 1 >>END_MODULE