FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n01_35005-1.3410000003578e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n01_35005-1.3410000003578e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2643498
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT224100.8477403803596598TruSeq Adapter, Index 2 (97% over 37bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC52040.19686037212814234No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG50540.19118607239347257No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTCG200.002060608771.369711
CGCGTGG301.2907709E-463.4397471
TCGTGCG301.3010895E-463.3377342
CGAGGCG1000.057.095771
TCGGAAG42800.052.7197843
CGGAAGA43450.052.3684274
GAAGAGC45800.049.5701756
GATCGGA45950.049.2883341
ATCGGAA46200.048.8399732
TCGCCGG554.963036E-543.184822
GTATGCG459.5481344E-442.2931631
ACCGCGA459.624058E-442.225163
CGTATGC27300.041.7556146-47
TGCCGTC27500.041.5390850-51
TATGCCG27500.041.4523348-49
ATGCCGT27350.041.33234448-49
GCCGTCT27650.041.14195350-51
TCGTATG28250.040.3510644-45
CTCGTAT27950.040.1044344-45
CGCGGAG951.9015715E-840.0672071
Produced by FastQC (version 0.11.2)