##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n01_34344-1.34100000035857.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4884870 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67203426089128 33.0 33.0 33.0 33.0 33.0 2 32.724136978056734 33.0 33.0 33.0 33.0 33.0 3 32.56925097290204 33.0 33.0 33.0 33.0 33.0 4 36.612276682900465 37.0 37.0 37.0 37.0 37.0 5 36.67354566242295 37.0 37.0 37.0 37.0 37.0 6 36.54556907348609 37.0 37.0 37.0 37.0 37.0 7 36.47670910382467 37.0 37.0 37.0 37.0 37.0 8 36.420851731980584 37.0 37.0 37.0 37.0 37.0 9 36.440641204371865 37.0 37.0 37.0 37.0 37.0 10-11 36.38894034027518 37.0 37.0 37.0 37.0 37.0 12-13 36.36375297602598 37.0 37.0 37.0 37.0 37.0 14-15 38.175885745168245 40.0 37.0 40.0 37.0 40.0 16-17 38.17467025734564 40.0 37.0 40.0 37.0 40.0 18-19 38.19573049026893 40.0 37.0 40.0 37.0 40.0 20-21 38.145630078180176 40.0 37.0 40.0 37.0 40.0 22-23 38.01819157930508 40.0 37.0 40.0 35.0 40.0 24-25 37.99164143569839 40.0 37.0 40.0 33.0 40.0 26-27 37.92412336459312 40.0 37.0 40.0 33.0 40.0 28-29 37.77684513610393 40.0 37.0 40.0 33.0 40.0 30-31 37.72416584269387 40.0 37.0 40.0 33.0 40.0 32-33 37.598035771678674 40.0 37.0 40.0 33.0 40.0 34-35 37.49372061078391 40.0 37.0 40.0 33.0 40.0 36-37 37.42568614927316 40.0 37.0 40.0 33.0 40.0 38-39 37.30589534624258 40.0 37.0 40.0 33.0 40.0 40-41 37.18094452871827 40.0 37.0 40.0 33.0 40.0 42-43 37.02953312984788 38.5 37.0 40.0 33.0 40.0 44-45 36.903253310732936 37.0 37.0 40.0 33.0 40.0 46-47 36.86873888148507 37.0 37.0 40.0 33.0 40.0 48-49 36.681143612829004 37.0 37.0 40.0 33.0 40.0 50-51 36.50649618106521 37.0 37.0 40.0 33.0 40.0 52-53 36.364270390000144 37.0 37.0 40.0 33.0 40.0 54-55 36.21050509020711 37.0 37.0 40.0 33.0 40.0 56-57 36.027751199110725 37.0 37.0 40.0 33.0 40.0 58-59 35.802603037542454 37.0 37.0 40.0 27.0 40.0 60-61 35.52053350447402 37.0 33.0 40.0 27.0 40.0 62-63 35.27233027695722 37.0 33.0 40.0 27.0 40.0 64-65 34.965535418547475 37.0 33.0 37.0 27.0 40.0 66-67 34.55907147989609 37.0 33.0 37.0 27.0 40.0 68-69 34.277930221275085 37.0 33.0 37.0 27.0 40.0 70-71 33.922059952465474 37.0 33.0 37.0 27.0 40.0 72-73 33.442316683964975 37.0 33.0 37.0 27.0 37.0 74-75 33.00590824320811 37.0 33.0 37.0 24.5 37.0 76-77 30.419970132265547 33.0 30.0 35.0 22.0 37.0 78-79 32.69243296136847 35.0 33.0 37.0 27.0 37.0 80-81 33.036624004323556 37.0 33.0 37.0 27.0 37.0 82-83 32.971065248409886 37.0 33.0 37.0 27.0 37.0 84-85 32.82144765367349 37.0 33.0 37.0 27.0 37.0 86-87 32.6714469371754 37.0 33.0 37.0 27.0 37.0 88-89 32.55182778661459 35.0 33.0 37.0 24.5 37.0 90-91 32.42385498488189 33.0 33.0 37.0 22.0 37.0 92-93 32.25408670036255 33.0 33.0 37.0 22.0 37.0 94-95 32.124995854546796 33.0 33.0 37.0 22.0 37.0 96-97 31.98524822564367 33.0 33.0 37.0 22.0 37.0 98-99 31.812081897778242 33.0 33.0 37.0 22.0 37.0 100-101 30.188534495288515 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 0.1191582007813281 2306 2 0.10892802047300165 2306 3 0.27828128666223506 2306 4 0.1954919328904623 2306 5 0.12166765290618287 2306 6 0.16780633563663372 2306 7 0.14007381877125624 2306 8 0.1440821525918352 2306 9 0.09634772639306277 2306 10-11 0.14487219138009522 2306 12-13 0.14708033403230303 2306 14-15 0.3271317424323925 2306 16-17 0.3436931466248865 2306 18-19 0.36265087732004275 2306 20-21 0.3328723253922661 2306 22-23 0.4431412102115857 2306 24-25 0.32458533522383703 2306 26-27 0.3744942779413023 2306 28-29 0.40061537886126786 2306 30-31 0.26815065990012243 2306 32-33 0.37219871296765206 2306 34-35 0.29710968668769056 2306 36-37 0.36833195846765676 2306 38-39 0.42655863900389335 2306 40-41 0.39661166408433957 2306 42-43 0.357577962312007 2306 44-45 0.30719314602215775 2306 46-47 0.39907730722304535 2306 48-49 0.4097202634244468 2306 50-51 0.34398830522572865 2306 52-53 0.4220764065638818 2306 54-55 0.4320691090888147 2306 56-57 0.46886533823269616 2306 58-59 0.4129871467648556 2306 60-61 0.44253372966064575 2306 62-63 0.40517907635981487 2306 64-65 0.25470292647619175 2306 66-67 0.26274094671751413 2306 68-69 0.28426411335736645 2306 70-71 0.295007004520599 2306 72-73 0.4822604406598501 2306 74-75 0.33876440211702885 2306 76-77 0.20950861564267953 2306 78-79 0.40676773378604736 2306 80-81 0.523665255363575 2306 82-83 0.502848169206203 2306 84-85 0.49432173008191427 2306 86-87 0.5341109132080462 2306 88-89 0.6655953438455882 2306 90-91 0.6180178853865215 2306 92-93 0.5350576951740393 2306 94-95 0.5929393544735575 2306 96-97 0.6153469890525898 2306 98-99 0.5708467621414322 2306 100-101 0.4484498756608737 2307 1 0.13701727145209475 2307 2 0.12810729945460508 2307 3 0.31955551668308857 2307 4 0.18379271356540272 2307 5 0.16411860218757823 2307 6 0.20177053007510892 2307 7 0.21319789254443577 2307 8 0.22366832253739233 2307 9 0.1328454703707891 2307 10-11 0.17151930755007072 2307 12-13 0.1770165209348349 2307 14-15 0.3109202059491025 2307 16-17 0.27363291393816525 2307 18-19 0.23422104373825192 2307 20-21 0.35379173852478374 2307 22-23 0.36621967464065364 2307 24-25 0.314587401684264 2307 26-27 0.40286935123068446 2307 28-29 0.38243825139755927 2307 30-31 0.36963467654667426 2307 32-33 0.4616051590155976 2307 34-35 0.37866958078023316 2307 36-37 0.3855945363415927 2307 38-39 0.3360258175420725 2307 40-41 0.38042320943687 2307 42-43 0.38894944864774317 2307 44-45 0.4296369235772346 2307 46-47 0.27463467903434235 2307 48-49 0.28676827655092296 2307 50-51 0.34268440868468986 2307 52-53 0.3008330662187575 2307 54-55 0.3966567111986734 2307 56-57 0.3734242643420984 2307 58-59 0.34689603567070293 2307 60-61 0.3643243821350808 2307 62-63 0.32850462548032766 2307 64-65 0.17927257746066516 2307 66-67 0.13427103797982198 2307 68-69 0.26359087618260446 2307 70-71 0.15642575227830235 2307 72-73 0.01578394469334654 2307 74-75 -0.0028905691469987005 2307 76-77 -0.2475133695333298 2307 78-79 0.1596507823040696 2307 80-81 0.09561842577185331 2307 82-83 0.23209937082476984 2307 84-85 0.19005428939223634 2307 86-87 0.21349712539154808 2307 88-89 0.21912525626910195 2307 90-91 0.07666286894025376 2307 92-93 0.13671886945425626 2307 94-95 0.14659376341317198 2307 96-97 0.2911597877306349 2307 98-99 0.05076879097300946 2307 100-101 -0.044582317865963006 2201 1 0.1350611874128802 2201 2 0.14682693299991456 2201 3 0.32423118908376836 2201 4 0.23892008751445104 2201 5 0.16105191972938115 2201 6 0.1499099140275888 2201 7 0.16398190074872332 2201 8 0.21414753831011524 2201 9 0.220782649762306 2201 10-11 0.17434595082153947 2201 12-13 0.2065813902200162 2201 14-15 0.31677271218070757 2201 16-17 0.35820826108361103 2201 18-19 0.403267421534089 2201 20-21 0.43199145660495475 2201 22-23 0.3784758786909066 2201 24-25 0.3007735529721103 2201 26-27 0.4034895166446333 2201 28-29 0.43984124818197046 2201 30-31 0.4598594593390217 2201 32-33 0.47297394546645677 2201 34-35 0.4368082698599096 2201 36-37 0.4100285573137441 2201 38-39 0.4302525370784025 2201 40-41 0.46194702098189566 2201 42-43 0.46680720715925617 2201 44-45 0.43311916050626564 2201 46-47 0.4672263765709346 2201 48-49 0.5268635152003682 2201 50-51 0.5363568388020568 2201 52-53 0.4879244861485361 2201 54-55 0.4621987051344547 2201 56-57 0.5995972301618906 2201 58-59 0.4738390266633701 2201 60-61 0.42922772635589723 2201 62-63 0.42549748643251206 2201 64-65 0.42604689578359967 2201 66-67 0.28247345682175506 2201 68-69 0.5025685848001871 2201 70-71 0.4555395669656477 2201 72-73 0.24499193004830744 2201 74-75 0.23539568969209768 2201 76-77 0.005140496868985878 2201 78-79 0.38730634310064715 2201 80-81 0.5868282848872539 2201 82-83 0.569506410194272 2201 84-85 0.6428773042638838 2201 86-87 0.6600595246264334 2201 88-89 0.5864544425011786 2201 90-91 0.4892168927329763 2201 92-93 0.5072020251215079 2201 94-95 0.5825185329926583 2201 96-97 0.5148771655066184 2201 98-99 0.4362697566065812 2201 100-101 0.2597165144054365 1101 1 0.11225115336777236 1101 2 0.11429729866518556 1101 3 0.2939795958114644 1101 4 0.15240161279008646 1101 5 0.13064815562162124 1101 6 0.18955166396725076 1101 7 0.1485265825339397 1101 8 0.13146857075181373 1101 9 0.174830331620484 1101 10-11 0.18092570237147498 1101 12-13 0.12873376873125153 1101 14-15 0.259660627758322 1101 16-17 0.2699063005120763 1101 18-19 0.2015426056514471 1101 20-21 0.28436578937638757 1101 22-23 0.27652843500503366 1101 24-25 0.2749269623783803 1101 26-27 0.23450785759586523 1101 28-29 0.2202196764692843 1101 30-31 0.2057521720398583 1101 32-33 0.3034808926263892 1101 34-35 0.3401955365286966 1101 36-37 0.2957874719365492 1101 38-39 0.3118844414402844 1101 40-41 0.333678856056153 1101 42-43 0.2365509053221615 1101 44-45 0.22243255821285146 1101 46-47 0.35250726272906263 1101 48-49 0.31850313100642325 1101 50-51 0.2980006361878367 1101 52-53 0.18739176349422593 1101 54-55 0.24573218365372895 1101 56-57 0.4594984064299794 1101 58-59 0.464987314647928 1101 60-61 0.46340114872163696 1101 62-63 0.4721592075470298 1101 64-65 0.2591890800961565 1101 66-67 0.2978427891597448 1101 68-69 0.30106843917769055 1101 70-71 0.17292285536493068 1101 72-73 0.26881188887487184 1101 74-75 0.17225071765972189 1101 76-77 -0.07103815668105184 1101 78-79 0.29701119404494847 1101 80-81 0.4382989474103809 1101 82-83 0.4204001955747714 1101 84-85 0.43920194203180785 1101 86-87 0.49146476304508013 1101 88-89 0.5635774439337027 1101 90-91 0.47080032382359605 1101 92-93 0.5019109703344 1101 94-95 0.365668388212093 1101 96-97 0.32734742515123116 1101 98-99 0.3009693813558023 1101 100-101 0.3467570629438832 2304 1 0.13587857227411604 2304 2 0.0831458653595476 2304 3 0.3087989924121217 2304 4 0.16326551199612993 2304 5 0.12406872200472208 2304 6 0.13244841927264872 2304 7 0.08677901535065757 2304 8 0.13841092922088194 2304 9 0.03110797631419615 2304 10-11 0.08901643037710727 2304 12-13 0.050578546783007994 2304 14-15 0.23964293403872716 2304 16-17 0.25807662375993345 2304 18-19 0.25788921652600294 2304 20-21 0.23929847152282235 2304 22-23 0.2702325081026302 2304 24-25 0.276638531320998 2304 26-27 0.2521075165510922 2304 28-29 0.3071108935623599 2304 30-31 0.27669693829394504 2304 32-33 0.23900424211667115 2304 34-35 0.34050930441242144 2304 36-37 0.34321191872194134 2304 38-39 0.28361147983682855 2304 40-41 0.32627839647683743 2304 42-43 0.3595531452064975 2304 44-45 0.3218237648469895 2304 46-47 0.40425480341638576 2304 48-49 0.4041528383097983 2304 50-51 0.3749019719465281 2304 52-53 0.29982372118548284 2304 54-55 0.3203743906496115 2304 56-57 0.3379248563095345 2304 58-59 0.2677721719588462 2304 60-61 0.33048970071214256 2304 62-63 0.3396663956206112 2304 64-65 0.3114512349758911 2304 66-67 0.19020970207144927 2304 68-69 0.34571659800169385 2304 70-71 0.22898003279829737 2304 72-73 0.1371604978233094 2304 74-75 0.22268269933679363 2304 76-77 -0.0028612066554920546 2304 78-79 0.2682565987399741 2304 80-81 0.24446800052584905 2304 82-83 0.2953073551442884 2304 84-85 0.3013659419503725 2304 86-87 0.34806722722463945 2304 88-89 0.48662736956374175 2304 90-91 0.37821355724208416 2304 92-93 0.5015739836272743 2304 94-95 0.3777126124705106 2304 96-97 0.2888853744703255 2304 98-99 0.2368828837779553 2304 100-101 0.015359015054254854 2202 1 0.130882453393248 2202 2 0.08060582513154912 2202 3 0.2872273501567477 2202 4 0.14943990162134924 2202 5 0.08749631898393062 2202 6 0.0752376751580428 2202 7 0.1319856851783996 2202 8 0.0945757564517109 2202 9 0.10269964904630768 2202 10-11 0.07887711961125632 2202 12-13 0.05119432733300755 2202 14-15 0.049024103570488364 2202 16-17 0.044636233081178034 2202 18-19 0.024869229619518762 2202 20-21 0.14855844841125787 2202 22-23 0.15510532501596686 2202 24-25 0.16657400681933154 2202 26-27 0.21187116004214346 2202 28-29 0.2492539507472955 2202 30-31 0.2508498629274314 2202 32-33 0.25639005390699054 2202 34-35 0.24644279475244701 2202 36-37 0.14487170878808087 2202 38-39 0.18692508230388682 2202 40-41 0.3125756430171407 2202 42-43 0.26110358576166703 2202 44-45 0.3160746409115447 2202 46-47 0.3787679462701874 2202 48-49 0.35765931865365275 2202 50-51 0.35709195785506864 2202 52-53 0.23060821712899582 2202 54-55 0.25535889765086495 2202 56-57 0.34379228690394825 2202 58-59 0.3950694513482418 2202 60-61 0.3259398497579653 2202 62-63 0.3444590931202711 2202 64-65 0.14704730263208887 2202 66-67 0.0886071891332918 2202 68-69 0.1861085109374656 2202 70-71 0.2657909502714233 2202 72-73 0.22128516536632503 2202 74-75 0.10206980851108938 2202 76-77 -0.12863679936842232 2202 78-79 0.2448800319481066 2202 80-81 0.20540249348052697 2202 82-83 0.29168722124583724 2202 84-85 0.26710784282172995 2202 86-87 0.3909778565169617 2202 88-89 0.35623516802953503 2202 90-91 0.38941834475976833 2202 92-93 0.38104062878515066 2202 94-95 0.25205800670438094 2202 96-97 0.3396159928881559 2202 98-99 0.1959696922551153 2202 100-101 0.10405229556638673 1102 1 0.06663748428142213 1102 2 0.02218174171882481 1102 3 0.23267946163037578 1102 4 0.1584705809232645 1102 5 0.06304751585123114 1102 6 0.03432357981721168 1102 7 -0.021977442108600087 1102 8 0.039615370240376535 1102 9 0.019348978683119356 1102 10-11 0.09100718301004207 1102 12-13 0.07655822540846202 1102 14-15 0.07503123783185828 1102 16-17 0.07707511107334852 1102 18-19 0.08096196818852519 1102 20-21 0.0755715542639237 1102 22-23 0.06613398368558165 1102 24-25 0.07590787515751884 1102 26-27 0.10762454141053013 1102 28-29 0.08984057932312339 1102 30-31 0.12407970508333932 1102 32-33 0.08367558954938659 1102 34-35 0.10395937148437895 1102 36-37 0.12009064692355054 1102 38-39 0.14578575274832417 1102 40-41 0.16287256835228447 1102 42-43 0.17586161910551112 1102 44-45 0.18257097493918195 1102 46-47 0.17235709527927412 1102 48-49 0.1496005079162117 1102 50-51 0.11571882270429512 1102 52-53 0.09810839167668206 1102 54-55 0.11427797828554276 1102 56-57 0.17822939151517403 1102 58-59 0.1589514966727137 1102 60-61 0.19180060061795956 1102 62-63 0.10035509613721416 1102 64-65 -0.07849368039929061 1102 66-67 0.02487020382673677 1102 68-69 -0.08719004354867366 1102 70-71 -0.16998391959489112 1102 72-73 -0.09803372542639721 1102 74-75 -0.15028016848864922 1102 76-77 -0.1100407596994124 1102 78-79 0.05455394154900972 1102 80-81 0.046056321443735726 1102 82-83 -0.004247401256094463 1102 84-85 -0.03788256676898527 1102 86-87 0.010302863837367227 1102 88-89 -0.02961974063404682 1102 90-91 -0.03983736035931429 1102 92-93 -0.0252476620615667 1102 94-95 -0.20098990201417877 1102 96-97 -0.15963333432323878 1102 98-99 -0.18141776981682156 1102 100-101 -0.08865959384490907 2305 1 0.0935606047335682 2305 2 0.03932877642001387 2305 3 0.2655687002214222 2305 4 0.11453769009606418 2305 5 0.04075171911955522 2305 6 -8.678992453567957E-4 2305 7 0.015555577385740094 2305 8 0.06637438610292179 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0.26508799803173844 1213 98-99 0.278198880096479 1213 100-101 0.5489868676153868 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 34.0 10 26.0 11 46.0 12 89.0 13 201.0 14 580.0 15 1205.0 16 2237.0 17 3845.0 18 5810.0 19 8107.0 20 10725.0 21 13753.0 22 17347.0 23 21818.0 24 27293.0 25 33961.0 26 42988.0 27 56340.0 28 77993.0 29 111243.0 30 121189.0 31 120040.0 32 154065.0 33 216659.0 34 331178.0 35 563137.0 36 1059763.0 37 1519055.0 38 363918.0 39 225.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.09519677231236 24.944902440204693 23.911069626747352 15.048831160735595 2 28.76504390086123 23.927801558690405 33.667815110739895 13.639339429708466 3 37.024943760006664 19.297692527680884 27.350457095164682 16.326906617147767 4 27.943385501472655 19.166078314456918 34.44911394976264 18.441422234307787 5 33.68427409531881 18.043632686233206 32.04476270606997 16.227330512378018 6 27.016501974464006 19.377854477191818 37.37534468675727 16.2302988615869 7 31.899190766591534 21.612263990648675 29.10785752742652 17.380687715333266 8 27.78884596724171 28.632901182631265 26.458882222044807 17.11937062808222 9 31.86993717335364 23.790704767987684 25.723202459840284 18.61615559881839 10-11 25.009219809155326 28.85251120169708 28.865223944379192 17.273045044768402 12-13 23.961917103218713 27.093648346834204 28.812414659960243 20.13201988998684 14-15 22.91282060730378 27.573794184901544 29.581708418033642 19.93167678976104 16-17 23.164219313922377 27.126433252061975 30.419714342449232 19.289633091566408 18-19 21.16534319234698 27.344944696583536 31.683483900288035 19.806228210781455 20-21 22.50008700333888 27.505563095844927 31.222499268148386 18.771850632667807 22-23 21.325460042948944 29.967409572823843 29.818091371930063 18.889039012297154 24-25 21.375225952788917 27.111110428731983 31.158792352713583 20.354871265765517 26-27 21.260969332033262 27.31963743551772 29.618327533450746 21.801065698998272 28-29 22.0435045977748 28.91434212177496 29.31633906621332 19.72581421423692 30-31 20.42990908662871 28.14332827690399 30.549728037798346 20.87703459866895 32-33 20.361923654058348 29.282672824455926 29.773402362805975 20.58200115867976 34-35 20.61731427857855 29.40453891301099 31.102690552665678 18.87545625574478 36-37 20.447081917933545 28.46312635119138 31.071682529277755 20.01810920159732 38-39 22.22802476276827 29.40849468052493 29.255203887706717 19.10827666900008 40-41 20.844168698628188 30.508348971811433 29.007730631812258 19.63975169774812 42-43 20.91031045240181 29.394653539246633 30.56541883980209 19.129617168549466 44-45 20.511926066860656 28.234974040123273 30.843224126205822 20.409875766810252 46-47 21.92268787414071 28.084272645179542 29.45838151957554 20.53465796110421 48-49 20.818003914695584 29.626949286860878 30.152051182157408 19.402995616286134 50-51 22.056180342295125 28.386610325480188 29.787756304476197 19.769453027748487 52-53 20.57626231395675 28.122667118148986 28.894810386126906 22.406260181767355 54-55 22.08504736505976 28.327799399761762 29.878839662237777 19.708313572940696 56-57 20.83764127136345 28.35850261055926 29.61580290205909 21.188053216018197 58-59 20.796760640218476 28.2878466781114 29.97347591441245 20.941916767257677 60-61 21.78433440864673 28.630052782993975 29.91833043392811 19.667282374431185 62-63 20.560703753962486 28.571318899952985 29.876614179506966 20.99136316657756 64-65 21.973481468803065 28.438537879492042 29.715532727126064 19.87244792457883 66-67 21.04509421721031 30.487956825724705 28.42724169573676 20.03970726132823 68-69 21.212576660390262 30.51248624615258 28.278319820682697 19.99661727277446 70-71 20.914990321205735 31.192613585695593 28.20570706389704 19.686689029201627 72-73 20.637742427271945 31.56586264451856 28.056547022134694 19.739847906074797 74-75 21.068215778850174 30.969759857238145 27.953710922602575 20.0083134413091 76-77 21.142514016127247 30.279677536929274 28.35186110417187 20.22594734277161 78-79 20.941319310968595 29.9872289740029 28.84369236722612 20.227759347802383 80-81 20.557965179201037 30.15358889822303 28.742764006476968 20.545681916098967 82-83 19.865182009200772 30.155801415326867 28.991088288424027 20.987928287048334 84-85 19.989649091721105 29.902353224540292 29.055087570710818 21.052910113027785 86-87 20.014643242937787 29.994529258231296 29.47983523894775 20.510992259883164 88-89 20.151586471712054 30.237893464824573 29.23195109331814 20.378568970145228 90-91 20.326321196237355 29.92759759336403 28.94798081759567 20.798100392802944 92-93 19.958302316193308 30.6087392931358 28.77985848710344 20.65309990356745 94-95 19.702018842612958 30.953856473965402 28.72352308394649 20.62060159947515 96-97 20.085347268472752 31.080411849714686 28.467163672533097 20.36707720927947 98-99 20.354132534303695 30.49429207742825 28.17794846533251 20.973626922935544 100-101 20.404927396459456 30.442368156208953 27.756267513562932 21.39643693376866 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 71.0 1 51.5 2 85.5 3 220.5 4 260.0 5 176.0 6 137.5 7 127.0 8 104.5 9 101.0 10 115.0 11 122.0 12 114.5 13 121.5 14 136.5 15 149.0 16 178.0 17 228.0 18 304.0 19 419.5 20 593.0 21 871.5 22 1360.0 23 2171.0 24 3257.0 25 4887.0 26 7726.5 27 11630.5 28 15893.5 29 20360.0 30 25916.5 31 32893.0 32 46078.5 33 69588.5 34 103968.5 35 145655.0 36 202108.0 37 271595.5 38 332281.0 39 355323.5 40 349948.5 41 327932.5 42 301524.0 43 298632.5 44 289659.0 45 266628.5 46 249895.5 47 220192.5 48 176603.5 49 145457.0 50 122175.5 51 102494.0 52 86787.0 53 71100.5 54 55117.5 55 40779.0 56 30518.5 57 22885.5 58 17733.5 59 13660.0 60 10302.0 61 7733.5 62 5668.0 63 4154.5 64 2991.5 65 2067.5 66 1421.0 67 999.5 68 716.0 69 529.0 70 373.0 71 267.5 72 182.0 73 113.5 74 73.0 75 46.0 76 45.5 77 34.5 78 21.0 79 18.5 80 12.5 81 7.0 82 9.0 83 7.0 84 2.0 85 1.0 86 1.0 87 1.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16512210150935439 2 0.0 3 2.047137385437074E-5 4 0.024033392905031252 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.5353530390778056E-4 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 2.047137385437074E-5 28-29 1.432996169805952E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.00123851811818943 38-39 7.472051456845321E-4 40-41 3.480133555243026E-4 42-43 6.34612589485493E-4 44-45 4.913129725048977E-4 46-47 0.0036234331722236213 48-49 0.004073803397019777 50-51 0.004616294804160602 52-53 0.004493466561034378 54-55 0.006335890207927745 56-57 0.009744373954680472 58-59 0.01675581949980245 60-61 0.005066665028956759 62-63 0.0031014131389371672 64-65 0.01048134341343782 66-67 0.023101945394657382 68-69 0.02531285377092942 70-71 0.025701809874162467 72-73 0.022753932039133076 74-75 0.02482154079842452 76-77 0.024770362363788597 78-79 0.015711779433229545 80-81 0.003572254737587694 82-83 0.001269225178970986 84-85 0.005619392123024768 86-87 0.014452789941185741 88-89 0.016233799466515996 90-91 0.022999588525385528 92-93 0.023449958750181684 94-95 0.02467824118144393 96-97 0.02645925070677418 98-99 0.03303056171402719 100-101 0.023644436801798206 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4884870.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.00663279788 #Duplication Level Percentage of deduplicated Percentage of total 1 91.1541456248081 42.84849451389834 2 3.9123052373271814 3.6780859136852313 3 1.3816853529201683 1.9484512808078274 4 0.7793738519126385 1.4654296187650682 5 0.44053771080867593 1.035409720280104 6 0.37512110827415973 1.0579908114826326 7 0.288998768881607 0.9509401305499963 8 0.1668260694784657 0.6273545431270283 9 0.13819476113994572 0.5846463352346548 >10 1.0565603707661881 9.928503165974076 >50 0.12952733562695298 4.291271725828211 >100 0.14049328368823344 14.201241665829759 >500 0.027241611441960297 8.891587634396934 >1k 0.008814098910825179 5.8019209396390545 >5k 1.3111062349566778E-4 0.4465962857371812 >10k+ 4.370339153189751E-5 2.2420757147637653 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT 109138 2.234204799718314 TruSeq Adapter, Index 13 (97% over 38bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 8125 0.16632991256676227 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 7581 0.15519348518998458 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 6033 0.12350379846341868 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0018424236468933667 0.0 0.0 2 0.001965251890019591 0.0 0.0 3 0.0021494942547089276 0.0 0.0 4 0.0022518511239807814 0.0 0.0 5 0.002374679367107006 0.0 0.0 6 0.00249750761023323 0.0 0.0 7 0.0027431640964856794 0.0 0.0 8 0.0032754198166993183 0.0 0.0 9 0.0037462614153498455 0.0 0.0 10-11 0.005588685062243212 0.0 0.0 12-13 0.007809829125442438 0.0 1.023568692718537E-5 14-15 0.010112858684059145 0.0 2.047137385437074E-5 16-17 0.012661544728928303 0.0 2.047137385437074E-5 18-19 0.015445651573122724 0.0 2.047137385437074E-5 20-21 0.018936020815292935 0.0 2.047137385437074E-5 22-23 0.022815346160696193 0.0 2.047137385437074E-5 24-25 0.027759182946526725 0.0 2.047137385437074E-5 26-27 0.03292820484475534 0.0 2.047137385437074E-5 28-29 0.039049145627212185 0.0 2.047137385437074E-5 30-31 0.05800563781635949 0.0 2.047137385437074E-5 32-33 0.06529344690851548 0.0 2.047137385437074E-5 34-35 0.07196711478504034 0.0 2.047137385437074E-5 36-37 0.07893761758245357 0.0 4.094274770874148E-5 38-39 0.08623566236153675 0.0 4.094274770874148E-5 40-41 0.09529424529209579 0.0 4.094274770874148E-5 42-43 0.10435282822265485 0.0 4.094274770874148E-5 44-45 0.11401531668191783 0.0 4.094274770874148E-5 46-47 0.12447618872150129 0.0 4.094274770874148E-5 48-49 0.1361346361315654 0.0 6.141412156311222E-5 50-51 0.14893948047747432 0.0 6.141412156311222E-5 52-53 0.16148843265020357 3.0707060781556115E-5 8.188549541748296E-5 54-55 0.17591051553060777 4.094274770874148E-5 1.023568692718537E-4 56-57 0.19114121767825962 4.094274770874148E-5 1.023568692718537E-4 58-59 0.20819387209895043 4.094274770874148E-5 1.023568692718537E-4 60-61 0.22611655990845203 4.094274770874148E-5 1.023568692718537E-4 62-63 0.24575884312172075 4.094274770874148E-5 1.023568692718537E-4 64-65 0.26709001467797505 4.094274770874148E-5 1.023568692718537E-4 66-67 0.2894549906138751 4.094274770874148E-5 1.023568692718537E-4 68-69 0.31574023464288714 4.094274770874148E-5 1.023568692718537E-4 70-71 0.3447891141422392 4.094274770874148E-5 1.023568692718537E-4 72-73 0.3745033132918583 4.094274770874148E-5 1.432996169805952E-4 74-75 0.40819919465615256 4.094274770874148E-5 1.6377099083496593E-4 76-77 0.4463680712076268 4.094274770874148E-5 1.6377099083496593E-4 78-79 0.48724940479480516 4.094274770874148E-5 1.6377099083496593E-4 80-81 0.5329108041769791 4.094274770874148E-5 1.6377099083496593E-4 82-83 0.5814893743334009 4.094274770874148E-5 1.6377099083496593E-4 84-85 0.6364140703846777 4.094274770874148E-5 1.6377099083496593E-4 86-87 0.6973573503491393 4.094274770874148E-5 1.6377099083496593E-4 88-89 0.7631932886647956 4.094274770874148E-5 1.6377099083496593E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAAGA 17265 0.0 68.351776 4 TCGGAAG 17215 0.0 68.19154 3 GATCGGA 17535 0.0 67.19058 1 ATCGGAA 17765 0.0 66.10709 2 GAAGAGC 18200 0.0 64.84515 6 GGAAGAG 20855 0.0 56.931458 5 TTGTGTT 58865 0.0 46.464703 1 ATGCCGT 13565 0.0 43.76693 48-49 GCCGTCT 13595 0.0 43.68894 50-51 TCGTATG 13665 0.0 43.671448 44-45 TCTCGTA 13470 0.0 43.509876 42-43 CGTCTTC 13725 0.0 43.276894 52-53 TATGCCG 13530 0.0 43.248272 48-49 AATCTCG 13405 0.0 43.136036 40-41 TGCCGTC 13595 0.0 43.112473 50-51 CGTATGC 13625 0.0 43.068073 46-47 CTCGTAT 13485 0.0 43.039284 44-45 GTATGCC 13880 0.0 42.79011 46-47 ATCTCGT 13525 0.0 42.54284 42-43 CCGTCTT 14110 0.0 41.49012 52-53 >>END_MODULE