FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n01_34344-1.34100000035857.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n01_34344-1.34100000035857.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4884870
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT1091382.234204799718314TruSeq Adapter, Index 13 (97% over 38bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC81250.16632991256676227No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA75810.15519348518998458No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG60330.12350379846341868No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA172650.068.3517764
TCGGAAG172150.068.191543
GATCGGA175350.067.190581
ATCGGAA177650.066.107092
GAAGAGC182000.064.845156
GGAAGAG208550.056.9314585
TTGTGTT588650.046.4647031
ATGCCGT135650.043.7669348-49
GCCGTCT135950.043.6889450-51
TCGTATG136650.043.67144844-45
TCTCGTA134700.043.50987642-43
CGTCTTC137250.043.27689452-53
TATGCCG135300.043.24827248-49
AATCTCG134050.043.13603640-41
TGCCGTC135950.043.11247350-51
CGTATGC136250.043.06807346-47
CTCGTAT134850.043.03928444-45
GTATGCC138800.042.7901146-47
ATCTCGT135250.042.5428442-43
CCGTCTT141100.041.4901252-53
Produced by FastQC (version 0.11.2)