##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n01_34311-1.34100000035764.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2654147 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.670916870844 33.0 33.0 33.0 33.0 33.0 2 32.72420329393964 33.0 33.0 33.0 33.0 33.0 3 32.55571903138748 33.0 33.0 33.0 33.0 33.0 4 36.61695716175479 37.0 37.0 37.0 37.0 37.0 5 36.67857808930704 37.0 37.0 37.0 37.0 37.0 6 36.565469810074575 37.0 37.0 37.0 37.0 37.0 7 36.51027995058299 37.0 37.0 37.0 37.0 37.0 8 36.44239561712294 37.0 37.0 37.0 37.0 37.0 9 36.454885128819164 37.0 37.0 37.0 37.0 37.0 10-11 36.411086688114864 37.0 37.0 37.0 37.0 37.0 12-13 36.39523413737069 37.0 37.0 37.0 37.0 37.0 14-15 38.22373534698718 40.0 37.0 40.0 37.0 40.0 16-17 38.22801845564696 40.0 37.0 40.0 37.0 40.0 18-19 38.255116992389645 40.0 37.0 40.0 37.0 40.0 20-21 38.19160355473906 40.0 37.0 40.0 37.0 40.0 22-23 38.09529426968439 40.0 37.0 40.0 37.0 40.0 24-25 38.05367336473827 40.0 37.0 40.0 35.0 40.0 26-27 37.98188193796349 40.0 37.0 40.0 35.0 40.0 28-29 37.86312834217547 40.0 37.0 40.0 33.0 40.0 30-31 37.82438048834522 40.0 37.0 40.0 33.0 40.0 32-33 37.718779329102716 40.0 37.0 40.0 33.0 40.0 34-35 37.6425716058681 40.0 37.0 40.0 33.0 40.0 36-37 37.56574089528576 40.0 37.0 40.0 33.0 40.0 38-39 37.469685552458095 40.0 37.0 40.0 33.0 40.0 40-41 37.40375533080873 40.0 37.0 40.0 33.0 40.0 42-43 37.27146235683254 40.0 37.0 40.0 33.0 40.0 44-45 37.14574098571029 40.0 37.0 40.0 33.0 40.0 46-47 37.10299033926907 40.0 37.0 40.0 33.0 40.0 48-49 36.92742696617783 37.0 37.0 40.0 33.0 40.0 50-51 36.729598059188135 37.0 37.0 40.0 33.0 40.0 52-53 36.61262959436685 37.0 37.0 40.0 33.0 40.0 54-55 36.4885234314452 37.0 37.0 40.0 33.0 40.0 56-57 36.30410203353469 37.0 37.0 40.0 33.0 40.0 58-59 36.04181437576743 37.0 37.0 40.0 33.0 40.0 60-61 35.76540692734803 37.0 37.0 40.0 30.0 40.0 62-63 35.523972862090915 37.0 33.0 40.0 27.0 40.0 64-65 35.21961349540926 37.0 33.0 37.0 27.0 40.0 66-67 34.9107637971823 37.0 33.0 37.0 27.0 40.0 68-69 34.64294705605982 37.0 33.0 37.0 27.0 40.0 70-71 34.311352197146576 37.0 33.0 37.0 27.0 40.0 72-73 33.975086157624276 37.0 33.0 37.0 27.0 37.0 74-75 33.62176039985728 37.0 33.0 37.0 27.0 37.0 76-77 31.025182478589166 33.0 30.0 35.0 24.5 37.0 78-79 33.357620923031014 35.0 33.0 37.0 27.0 37.0 80-81 33.75291515503851 37.0 33.0 37.0 27.0 37.0 82-83 33.72757066582974 37.0 33.0 37.0 27.0 37.0 84-85 33.58702004824902 37.0 33.0 37.0 27.0 37.0 86-87 33.470046685432266 37.0 33.0 37.0 27.0 37.0 88-89 33.38075792335542 37.0 33.0 37.0 27.0 37.0 90-91 33.27267724809515 37.0 33.0 37.0 27.0 37.0 92-93 33.12017495639842 37.0 33.0 37.0 27.0 37.0 94-95 33.01407552030841 35.0 33.0 37.0 27.0 37.0 96-97 32.88504461131957 33.0 33.0 37.0 27.0 37.0 98-99 32.727802755461546 33.0 33.0 37.0 27.0 37.0 100-101 31.123484682649455 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 0.16415050343721305 2306 2 0.16240323308920068 2306 3 0.35470835997843864 2306 4 0.23586665837884624 2306 5 0.14529830242303632 2306 6 0.17491210649320266 2306 7 0.22391934292271998 2306 8 0.17019140184295622 2306 9 0.19014842266442145 2306 10-11 0.20533589648063355 2306 12-13 0.18415021512902285 2306 14-15 0.40069423840303386 2306 16-17 0.41652407399329405 2306 18-19 0.4360381210685489 2306 20-21 0.3934539431533963 2306 22-23 0.4970034968811987 2306 24-25 0.4896016219371333 2306 26-27 0.5119875852955644 2306 28-29 0.6231250852168273 2306 30-31 0.596242584527026 2306 32-33 0.5298632602920463 2306 34-35 0.634996095718158 2306 36-37 0.6401314349491045 2306 38-39 0.7357675798804095 2306 40-41 0.7662232410326837 2306 42-43 0.6941443786476569 2306 44-45 0.5474238656533359 2306 46-47 0.7805592077500663 2306 48-49 0.6590095320337142 2306 50-51 0.5868673674592202 2306 52-53 0.6889402371447844 2306 54-55 0.6464019832513017 2306 56-57 0.6685968617987896 2306 58-59 0.4920647874301025 2306 60-61 0.5464463712479031 2306 62-63 0.6140184130343727 2306 64-65 0.4980936945952621 2306 66-67 0.48733308810079023 2306 68-69 0.4780217527569093 2306 70-71 0.5896582646079409 2306 72-73 0.5481841965647689 2306 74-75 0.6442846565343885 2306 76-77 0.46561016107040487 2306 78-79 0.585408713768949 2306 80-81 0.5319829045284123 2306 82-83 0.43122466203659116 2306 84-85 0.48409155537589754 2306 86-87 0.5729378863397088 2306 88-89 0.6091383739849192 2306 90-91 0.5141443186670926 2306 92-93 0.5109897569899928 2306 94-95 0.4870327846235014 2306 96-97 0.46857954232368115 2306 98-99 0.38413673677090543 2306 100-101 0.38380337709043744 2307 1 0.12451334882771903 2307 2 0.10806021233958774 2307 3 0.24644236008648335 2307 4 0.16302085995251048 2307 5 0.143597189037159 2307 6 0.1647809454695377 2307 7 0.11262099541711734 2307 8 0.22121653488369475 2307 9 0.2000298739136852 2307 10-11 0.1802794775062182 2307 12-13 0.19183168467931466 2307 14-15 0.36484324623388886 2307 16-17 0.4235931206752426 2307 18-19 0.43327918636462215 2307 20-21 0.39275111762941606 2307 22-23 0.3883235189747225 2307 24-25 0.3449785954987661 2307 26-27 0.42780341965143975 2307 28-29 0.5562216076912563 2307 30-31 0.4140200462629977 2307 32-33 0.531715136643264 2307 34-35 0.3836891158614151 2307 36-37 0.4819744694796171 2307 38-39 0.4282996039187026 2307 40-41 0.49593087663942015 2307 42-43 0.4845609805441242 2307 44-45 0.29705812076301896 2307 46-47 0.35471391535811136 2307 48-49 0.4772693250116262 2307 50-51 0.38898119101749273 2307 52-53 0.41851640303585924 2307 54-55 0.41208463906082926 2307 56-57 0.42223997857366413 2307 58-59 0.295948904455237 2307 60-61 0.44132103678564505 2307 62-63 0.4527436584169422 2307 64-65 0.3823603802244335 2307 66-67 0.4290638910417428 2307 68-69 0.38911156779528255 2307 70-71 0.27027312393810377 2307 72-73 0.223441596077258 2307 74-75 0.17020210568013994 2307 76-77 -0.25099956294792847 2307 78-79 0.2912318575102688 2307 80-81 0.347705359555853 2307 82-83 0.3604413120008161 2307 84-85 0.35145046384100453 2307 86-87 0.3398974796600598 2307 88-89 0.39667724988301956 2307 90-91 0.4218222603088648 2307 92-93 0.4993719132697194 2307 94-95 0.3882631978408142 2307 96-97 0.3861510624002378 2307 98-99 0.3209113794874767 2307 100-101 0.15414673078641528 2201 1 0.13692069123639072 2201 2 0.12517343653005497 2201 3 0.2998394450467359 2201 4 0.19433644361341607 2201 5 0.14503708642869384 2201 6 0.12863568574671547 2201 7 0.11369557831535104 2201 8 0.14673097293776038 2201 9 0.20515374344130777 2201 10-11 0.2042335493428311 2201 12-13 0.20363583338974678 2201 14-15 0.37389248674384135 2201 16-17 0.27508343389418854 2201 18-19 0.3407314321142323 2201 20-21 0.33994885204845104 2201 22-23 0.34594218307423574 2201 24-25 0.3073900401100431 2201 26-27 0.3359055853716839 2201 28-29 0.33797471917553423 2201 30-31 0.34469901177003237 2201 32-33 0.471031787362314 2201 34-35 0.39424306370955264 2201 36-37 0.41954404307870874 2201 38-39 0.35425314756637505 2201 40-41 0.28893130530746447 2201 42-43 0.40031496781224973 2201 44-45 0.46038197776557155 2201 46-47 0.5153934294437121 2201 48-49 0.5909691525249343 2201 50-51 0.5129479335627707 2201 52-53 0.5230116403543477 2201 54-55 0.6113405140703563 2201 56-57 0.5372434084034268 2201 58-59 0.5831642564994652 2201 60-61 0.5611863049167667 2201 62-63 0.4960675299835344 2201 64-65 0.5187804632491364 2201 66-67 0.35661729370958284 2201 68-69 0.4947352749339373 2201 70-71 0.3836469598470842 2201 72-73 0.4866304202209264 2201 74-75 0.3927629821242249 2201 76-77 0.15468570150335736 2201 78-79 0.3716429176310925 2201 80-81 0.4808956255387855 2201 82-83 0.5652252084524818 2201 84-85 0.6756008102950801 2201 86-87 0.6543120128172646 2201 88-89 0.5979374999280438 2201 90-91 0.5513610804959725 2201 92-93 0.39224883893830054 2201 94-95 0.504119230433183 2201 96-97 0.5715991418654838 2201 98-99 0.39803084679341083 2201 100-101 0.271350543261871 1101 1 0.12182621050357767 1101 2 0.09844564467590544 1101 3 0.2923336634627489 1101 4 0.20344506585562527 1101 5 0.1300094971767578 1101 6 0.12236600298653144 1101 7 0.09017579039850432 1101 8 0.09421628294145279 1101 9 0.024508886339397407 1101 10-11 0.038083007742486075 1101 12-13 0.1296704622732534 1101 14-15 0.2411439041812855 1101 16-17 0.19059566121560323 1101 18-19 0.2241237871147561 1101 20-21 0.2124455603140305 1101 22-23 0.26757172922715 1101 24-25 0.30177614935080754 1101 26-27 0.15301080543885348 1101 28-29 0.21544787490423545 1101 30-31 0.2658729758404732 1101 32-33 0.258863042453207 1101 34-35 0.3572898173707628 1101 36-37 0.23801531260701125 1101 38-39 0.33257333989796933 1101 40-41 0.24908175075130146 1101 42-43 0.19488228793280626 1101 44-45 0.23253426458323645 1101 46-47 0.3020738258965139 1101 48-49 0.2742118610631934 1101 50-51 0.18871203518114754 1101 52-53 0.13896929990504958 1101 54-55 0.2501110718779529 1101 56-57 0.4254511371101657 1101 58-59 0.40632489755211765 1101 60-61 0.4036648804812657 1101 62-63 0.3932744854897905 1101 64-65 0.24581456234282228 1101 66-67 0.17245482730919548 1101 68-69 0.2472756995694212 1101 70-71 0.20067088818401402 1101 72-73 0.346899923210259 1101 74-75 0.2876019095286253 1101 76-77 -0.16191760019510681 1101 78-79 0.22483783359982112 1101 80-81 0.33039943243050374 1101 82-83 0.2196362951580113 1101 84-85 0.3431077624799741 1101 86-87 0.5132201115871808 1101 88-89 0.7068629602889231 1101 90-91 0.4691540053747545 1101 92-93 0.4307380344544356 1101 94-95 0.4823458211439444 1101 96-97 0.4127691528352244 1101 98-99 0.3389532431528437 1101 100-101 0.31078899392510806 2304 1 0.12348519791968471 2304 2 0.0906606306812563 2304 3 0.3366009813020696 2304 4 0.16092642803653234 2304 5 0.1191312827936315 2304 6 0.12007612691434133 2304 7 0.08971842326951673 2304 8 0.1298922220425638 2304 9 0.12062938802726819 2304 10-11 0.1618844278826188 2304 12-13 0.0940579377978139 2304 14-15 0.18146878686422951 2304 16-17 0.23890044928044318 2304 18-19 0.30006214983598767 2304 20-21 0.29767916068374944 2304 22-23 0.265779505945126 2304 24-25 0.18981632468790366 2304 26-27 0.22748693982226342 2304 28-29 0.26668341767262405 2304 30-31 0.28779239322512495 2304 32-33 0.2835242737046286 2304 34-35 0.2575104872376883 2304 36-37 0.2622504496955358 2304 38-39 0.32220396273383045 2304 40-41 0.25599926569717013 2304 42-43 0.31434208653491424 2304 44-45 0.31304076067324615 2304 46-47 0.41202685998231203 2304 48-49 0.307921295248363 2304 50-51 0.21661066994244038 2304 52-53 0.2118464953107022 2304 54-55 0.3363044699810729 2304 56-57 0.374991337639365 2304 58-59 0.33079981576462814 2304 60-61 0.2929976717498519 2304 62-63 0.2907676749327308 2304 64-65 0.3388811720231999 2304 66-67 0.17728335155530317 2304 68-69 0.35040134566021663 2304 70-71 0.19641852490334344 2304 72-73 0.07021170425779388 2304 74-75 0.06821021649709991 2304 76-77 -0.09097161903185835 2304 78-79 0.20604104359605913 2304 80-81 0.11465418896177937 2304 82-83 0.2806568015368285 2304 84-85 0.3674841708493233 2304 86-87 0.30003382200299455 2304 88-89 0.4288828855117899 2304 90-91 0.25506503465494035 2304 92-93 0.2827064722134125 2304 94-95 0.20032197811338648 2304 96-97 0.17933340053796343 2304 98-99 0.19017688482025363 2304 100-101 0.38867952811948925 2202 1 0.11086884820173992 2202 2 0.06512070065178222 2202 3 0.27636465478519767 2202 4 0.18268814871150596 2202 5 0.1277528695879937 2202 6 0.084091504513367 2202 7 0.07848595033488692 2202 8 0.07765077782156027 2202 9 0.10331658060537308 2202 10-11 0.07663578153372441 2202 12-13 0.08753301806802227 2202 14-15 0.05763649268194371 2202 16-17 0.0864547713223871 2202 18-19 -0.005354520656716488 2202 20-21 0.11649345719838067 2202 22-23 0.13603350109064394 2202 24-25 0.1439039072154671 2202 26-27 0.17501287268606092 2202 28-29 0.21471974357732648 2202 30-31 0.1891983462291691 2202 32-33 0.18064698076197772 2202 34-35 0.16107891510468875 2202 36-37 0.21792526275547885 2202 38-39 0.19689099308806846 2202 40-41 0.23840390560608427 2202 42-43 0.23038262260829612 2202 44-45 0.2182937916580059 2202 46-47 0.24447963140887197 2202 48-49 0.2773202863995081 2202 50-51 0.2728692593905464 2202 52-53 0.2895970699811272 2202 54-55 0.3141526850593195 2202 56-57 0.24074648977781266 2202 58-59 0.2986940274331076 2202 60-61 0.2602506399876461 2202 62-63 0.09218059132009415 2202 64-65 0.19477510209552662 2202 66-67 0.2192245362181069 2202 68-69 0.17939442512848558 2202 70-71 0.17772088418943355 2202 72-73 0.2736415914050738 2202 74-75 0.27841199391982485 2202 76-77 0.0023304479451766724 2202 78-79 0.19356357894096732 2202 80-81 0.24620091708987246 2202 82-83 0.47605163302295495 2202 84-85 0.38379991360510246 2202 86-87 0.41069920006113847 2202 88-89 0.352467596982855 2202 90-91 0.4723912714589389 2202 92-93 0.4100876049645805 2202 94-95 0.5933098893738702 2202 96-97 0.5616254402430272 2202 98-99 0.530986290132013 2202 100-101 0.4744232633701273 1102 1 0.14811293625709965 1102 2 0.08781504315825117 1102 3 0.2953027706490161 1102 4 0.14920156953747465 1102 5 0.043229343988464564 1102 6 0.0035817523554513286 1102 7 0.03417324675660183 1102 8 0.09736776209874876 1102 9 0.05327978035507641 1102 10-11 0.0322674837495498 1102 12-13 0.04744565731456163 1102 14-15 0.12635780030163346 1102 16-17 0.10489144027143738 1102 18-19 0.13804702411118086 1102 20-21 0.09759197672559594 1102 22-23 0.12406838237915707 1102 24-25 0.12899513483445446 1102 26-27 0.08437032089918972 1102 28-29 0.033466329616828716 1102 30-31 0.01685007994409915 1102 32-33 0.02061360958428793 1102 34-35 0.1192400163059304 1102 36-37 0.08096403655428475 1102 38-39 0.1514254653424345 1102 40-41 0.13515520735311526 1102 42-43 0.06789431387745992 1102 44-45 0.2453841063230371 1102 46-47 0.23141508461247184 1102 48-49 0.28138727274660624 1102 50-51 0.29746946652704054 1102 52-53 0.20662670178445097 1102 54-55 0.23079544713333888 1102 56-57 0.3037080728077157 1102 58-59 0.22555632342435672 1102 60-61 0.1811254308453556 1102 62-63 0.16540008383933014 1102 64-65 0.1726019896149822 1102 66-67 -0.009566228439439328 1102 68-69 0.13941962661397866 1102 70-71 0.007503515423813667 1102 72-73 -0.02172758526213414 1102 74-75 0.04511425186888829 1102 76-77 0.008239919695810727 1102 78-79 0.09063992819494615 1102 80-81 0.16689537023521694 1102 82-83 0.12681863156962692 1102 84-85 0.11213896389121203 1102 86-87 0.24911596866578378 1102 88-89 0.10851903087373671 1102 90-91 0.23684549022571844 1102 92-93 0.20955930430817915 1102 94-95 0.15291310610773934 1102 96-97 0.012836258234372622 1102 98-99 -0.061428407107619876 1102 100-101 0.004758740972896192 2305 1 0.12060857072456344 2305 2 0.04852222921754645 2305 3 0.27867447233057874 2305 4 0.11352202681568002 2305 5 0.019054263475354105 2305 6 0.04033077501996729 2305 7 0.0382215606640699 2305 8 0.0028789070465151667 2305 9 0.04435536297995668 2305 10-11 0.07225515754004164 2305 12-13 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1213 94-95 0.1592312488248453 1213 96-97 0.0641918884760102 1213 98-99 0.05035804625914153 1213 100-101 0.40026373893903155 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 5.0 8 40.0 9 109.0 10 103.0 11 44.0 12 46.0 13 122.0 14 289.0 15 859.0 16 1617.0 17 2568.0 18 3267.0 19 3860.0 20 4634.0 21 5622.0 22 7063.0 23 8979.0 24 11091.0 25 14144.0 26 18105.0 27 23477.0 28 30794.0 29 38601.0 30 45189.0 31 56239.0 32 76427.0 33 109965.0 34 169808.0 35 300700.0 36 601340.0 37 912245.0 38 206703.0 39 92.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.929000487611205 24.321231618340825 25.112995970791633 13.636771923256338 2 32.442701930224665 21.028187210429568 33.73569738224748 12.793413477098293 3 38.63050487802857 17.624802855607793 27.844888713733155 15.899803552630488 4 31.630811878621063 17.12441409208863 34.80886532653697 16.435908702753345 5 30.48568899913984 16.03799638829349 37.321256132384526 16.155058480182145 6 25.36084097828794 16.893826905593397 41.63062558328532 16.11470653283334 7 34.29210966837933 17.871730540923316 30.13766758208946 17.698492208607888 8 30.801685061151474 27.738516367028655 24.402793063082036 17.057005508737834 9 29.212247852134794 26.47694343983208 25.437965568598877 18.872843139434252 10-11 24.668165328539825 27.439622281446134 30.16422799729706 17.727984392716976 12-13 24.33342991175696 25.99543280760259 29.648715764424505 20.022421516215942 14-15 23.199110674729017 26.914372112772956 30.754928042794916 19.13158916970311 16-17 22.27203316168999 27.885908353983407 31.565715840155047 18.276342644171557 18-19 20.94897155281904 28.10238091560113 31.90802920863087 19.040618322948955 20-21 21.524862790192103 28.064986603982373 31.552679636809867 18.857470969015658 22-23 20.831608045824137 28.33036376658866 31.778590258941954 19.05943792864525 24-25 21.580907161509895 26.984827893858178 31.931219333367743 19.503045611264184 26-27 21.35049817332992 27.365991289478558 31.645276551237846 19.638233985953676 28-29 21.0939978433741 28.6341067899053 30.920378909072827 19.351516457647772 30-31 20.11857293510872 28.930311697129056 30.899042140469234 20.052073227292986 32-33 19.63966200817061 29.80955840049553 31.360772406351266 19.190007184982598 34-35 20.151502535466197 29.48981348809994 31.60829072391243 18.750393252521434 36-37 20.459081704720333 30.21753501013234 31.31541116477392 18.007972120373402 38-39 21.01855668394032 30.249067682238074 30.378770809957885 18.353604823863716 40-41 21.17106127993649 29.54518780945764 29.859300914665564 19.424449995940304 42-43 20.45232079465468 29.68619896357908 30.9138498659073 18.947630375858942 44-45 19.865003480408454 29.22199919559707 31.824459312188402 19.088538011806072 46-47 20.494372327809433 29.00495370793375 31.23181450393687 19.26885946031994 48-49 20.527975310311906 30.6385661081583 29.81032669338921 19.023131888140583 50-51 20.784846836707647 29.711743935157127 29.782089349225792 19.721319878909433 52-53 19.94471852206446 29.520768891810206 29.78216151256936 20.752351073555978 54-55 21.176190211539783 29.28099834494634 30.04929365851637 19.493517784997508 56-57 21.01514547200521 29.448258230212907 29.359407708404493 20.17718858937739 58-59 20.522960272361246 29.54048931644585 30.315469282956524 19.621081128236376 60-61 20.40666266647953 29.838859023546156 30.388228523092604 19.36624978688171 62-63 20.34217000930639 29.869823555342883 29.85725804324646 19.93074839210426 64-65 20.941321652300882 29.651961754038908 29.598907258254435 19.807809335405775 66-67 21.125008714789896 29.988958125670095 29.13771629166376 19.748316867876248 68-69 21.906138541921052 30.076194740158524 28.65802965482102 19.359637063099406 70-71 20.951071968903978 31.087183010399443 28.80427562744999 19.157469393246586 72-73 20.222804337904964 31.222529610456075 28.89786055528108 19.656805496357883 74-75 21.07071010845372 30.148706492073433 28.75540516197208 20.02517823750076 76-77 21.303786792166985 29.283101550187183 29.14891957221557 20.26419208543026 78-79 21.097744817062313 29.289775548129114 29.65989588622662 19.95258374858195 80-81 19.72872622308781 29.819185353521938 30.051225070613206 20.400863352777044 82-83 19.07239897427628 29.690451772071107 30.12017892142272 21.116970332229894 84-85 19.064357386006222 29.212979351477035 30.64848340184901 21.074179860667734 86-87 19.206969889492562 29.279440702581017 31.61693718869618 19.896652219230237 88-89 19.561639540770617 29.85972638647115 31.049326668190318 19.529307404567913 90-91 19.971257003914804 29.71568665875766 30.021733428402165 20.291322908925366 92-93 19.140138910348934 30.625756074030246 30.19555230619067 20.038552709430153 94-95 18.953476408824518 30.841471416118736 30.360328038256306 19.84472413680044 96-97 19.54487536306821 31.560676061561143 29.419482804689935 19.474965770680708 98-99 20.008657261395776 30.685544295629835 28.887871693129668 20.41792674984472 100-101 19.559096750366493 30.692568013174903 28.590594415740544 21.15774082071806 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1625.0 1 993.5 2 429.0 3 712.5 4 798.0 5 580.5 6 510.5 7 530.5 8 542.0 9 543.5 10 529.5 11 502.0 12 467.5 13 462.5 14 482.0 15 498.5 16 506.0 17 532.5 18 561.5 19 583.5 20 672.5 21 829.0 22 1016.5 23 1285.0 24 1722.0 25 2415.0 26 3619.0 27 5671.5 28 7763.0 29 9796.5 30 12307.0 31 15552.0 32 23092.5 33 35018.5 34 51352.0 35 78566.5 36 125030.0 37 186614.5 38 235988.5 39 241657.0 40 221161.5 41 196054.5 42 169641.5 43 156907.0 44 140286.5 45 120130.0 46 115254.0 47 103612.5 48 78271.5 49 59781.5 50 48490.5 51 40627.0 52 35124.5 53 28921.0 54 22775.0 55 16625.0 56 11581.5 57 8614.5 58 6935.0 59 5433.5 60 4183.5 61 3285.0 62 2489.0 63 1826.5 64 1329.0 65 927.0 66 619.5 67 443.0 68 338.5 69 265.0 70 200.0 71 142.0 72 99.5 73 65.5 74 46.5 75 35.5 76 19.5 77 12.5 78 10.5 79 10.0 80 7.0 81 3.5 82 2.5 83 1.5 84 2.5 85 3.0 86 2.0 87 1.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16935761282250003 2 0.0 3 3.767688828086764E-5 4 0.021588856984937156 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 5.651533242130146E-5 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.883844414043382E-5 28-29 3.767688828086764E-5 30-31 0.0 32-33 0.0 34-35 0.0 36-37 8.477299863195218E-4 38-39 3.3909199452780876E-4 40-41 2.825766621065073E-4 42-43 4.897995476512793E-4 44-45 1.6954599726390438E-4 46-47 0.002806928176924639 48-49 0.003146020171452448 50-51 0.0037865272722271974 52-53 0.0035227890542611244 54-55 0.00582107923939405 56-57 0.00947573740263821 58-59 0.017538591494743884 60-61 0.003937234825350668 62-63 0.0021475826320094555 64-65 0.010248113612395998 66-67 0.023058255627890992 68-69 0.0257333146958326 70-71 0.02594053758137737 72-73 0.022907548074767525 74-75 0.025055130706776983 76-77 0.02490442315365351 78-79 0.015654747080700502 80-81 0.002844605065205507 82-83 7.912146538982203E-4 84-85 0.004954510808934095 86-87 0.01465630954125751 88-89 0.016596669287722195 90-91 0.02377411650522748 92-93 0.024000177834912685 94-95 0.025469576477866525 96-97 0.027127359562224698 98-99 0.03326869235200613 100-101 0.023246640069295332 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2654147.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.172060841797304 #Duplication Level Percentage of deduplicated Percentage of total 1 86.37819879537214 33.8361205861718 2 5.840789728939357 4.575915412523146 3 2.020503198955814 2.3744182272162973 4 1.1895672911654676 1.863912092197828 5 0.8638640331005779 1.6919667231828122 6 0.5453089262375999 1.2816524661692654 7 0.347425816892897 0.9526569666137784 8 0.3475931785937915 1.0892752912055774 9 0.24173660228072388 0.8522388803006885 >10 1.7747607871427606 13.903665981701755 >50 0.21027808775582446 5.71728136076408 >100 0.20165383084809244 17.80355861751824 >500 0.031219557628330914 8.057598498036008 >1k 0.006520863014071557 4.259605727904566 >5k 4.8275172750204083E-4 1.0199880784710518 >10k+ 9.655034513769505E-5 0.720145090023313 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT 19041 0.7174056297560006 TruSeq Adapter, Index 6 (97% over 36bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5890 0.22191687197431037 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 5544 0.20888066862913018 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 5442 0.2050376260244817 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 5089 0.1917376844613354 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 5004 0.18853514895746168 No Hit TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT 4144 0.1561330250359155 No Hit GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT 4060 0.1529681664203226 No Hit TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA 3769 0.14200419193059013 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 3768 0.14196651504230925 No Hit GTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGT 3465 0.13055041789320637 No Hit TGTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAG 3088 0.11634623101131926 No Hit GTGTTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAAT 3038 0.11446238659727588 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 4.521226593704117E-4 0.0 0.0 2 4.521226593704117E-4 0.0 0.0 3 4.897995476512793E-4 0.0 0.0 4 5.27476435932147E-4 0.0 0.0 5 5.27476435932147E-4 0.0 0.0 6 6.028302124938822E-4 0.0 0.0 7 6.781839890556175E-4 0.0 0.0 8 9.042453187408234E-4 0.0 0.0 9 0.0011303066484260292 0.0 0.0 10-11 0.0016012677519368747 0.0 0.0 12-13 0.002298290185132926 0.0 0.0 14-15 0.0033155661687163523 0.0 0.0 16-17 0.005011026141355396 0.0 0.0 18-19 0.007196285661645719 0.0 0.0 20-21 0.008401946086633484 0.0 0.0 22-23 0.009701798732323418 0.0 0.0 24-25 0.01128422804011986 0.0 0.0 26-27 0.012960849568618468 0.0 0.0 28-29 0.014505601988134042 0.0 0.0 30-31 0.021193249657988047 0.0 0.0 32-33 0.02362340895210401 0.0 0.0 34-35 0.025865183804815634 0.0 0.0 36-37 0.02776786666299945 0.0 0.0 38-39 0.029802418630166302 0.0 0.0 40-41 0.032194901036001394 0.0 0.0 42-43 0.03517137521018994 0.0 0.0 44-45 0.038355072269923254 0.0 0.0 46-47 0.041143162002707456 0.0 0.0 48-49 0.04491085083079423 0.0 0.0 50-51 0.04905530854168967 0.0 0.0 52-53 0.05367072735609595 0.0 0.0 54-55 0.058286146170502234 0.0 0.0 56-57 0.06371161808294717 0.0 0.0 58-59 0.0695138588782008 0.0 0.0 60-61 0.0759754452183696 0.0 0.0 62-63 0.08398178397805398 0.0 1.883844414043382E-5 64-65 0.09281701427991743 0.0 3.767688828086764E-5 66-67 0.10323467388957733 0.0 7.535377656173528E-5 68-69 0.11514057058633151 0.0 7.535377656173528E-5 70-71 0.12749858994245608 0.0 7.535377656173528E-5 72-73 0.14223025326027533 0.0 7.535377656173528E-5 74-75 0.15971232942259792 0.0 7.535377656173528E-5 76-77 0.17853193511889132 0.0 7.535377656173528E-5 78-79 0.2011945834198332 0.0 7.535377656173528E-5 80-81 0.22764375899300227 0.0 7.535377656173528E-5 82-83 0.2595560833668972 0.0 7.535377656173528E-5 84-85 0.29499119679505315 0.0 7.535377656173528E-5 86-87 0.3356633976942498 0.0 7.535377656173528E-5 88-89 0.3838521378054795 0.0 7.535377656173528E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAGTCG 15 6.5790734E-4 95.14432 1 CGGAAGA 3860 0.0 58.452633 4 TCGGAAG 3880 0.0 58.0289 3 GAAGAGC 4085 0.0 55.806072 6 GATCGGA 4135 0.0 54.992725 1 ATCGGAA 4165 0.0 54.286232 2 CGAGTGG 100 1.2732926E-11 47.572163 1 CGACGGG 130 0.0 47.572163 1 GGAAGAG 4825 0.0 47.345646 5 CGAGGCG 105 2.0008883E-11 45.306816 1 GCCGTCT 2540 0.0 44.039112 50-51 ATGCCGT 2575 0.0 43.715157 48-49 TCGTATG 2595 0.0 43.65237 44-45 TATGCCG 2540 0.0 43.476055 48-49 TGCCGTC 2545 0.0 43.39269 50-51 CGTATGC 2555 0.0 43.034515 46-47 AATCTCG 2530 0.0 42.989212 40-41 CTCGTAT 2510 0.0 42.859917 44-45 TCTCGTA 2560 0.0 42.672165 42-43 ATCTCGT 2575 0.0 41.87024 42-43 >>END_MODULE