FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n01_34311-1.34100000035764.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n01_34311-1.34100000035764.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2654147
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT190410.7174056297560006TruSeq Adapter, Index 6 (97% over 36bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58900.22191687197431037No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC55440.20888066862913018No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG54420.2050376260244817No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG50890.1917376844613354No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC50040.18853514895746168No Hit
TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT41440.1561330250359155No Hit
GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT40600.1529681664203226No Hit
TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA37690.14200419193059013No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA37680.14196651504230925No Hit
GTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGT34650.13055041789320637No Hit
TGTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAG30880.11634623101131926No Hit
GTGTTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAAT30380.11446238659727588No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTCG156.5790734E-495.144321
CGGAAGA38600.058.4526334
TCGGAAG38800.058.02893
GAAGAGC40850.055.8060726
GATCGGA41350.054.9927251
ATCGGAA41650.054.2862322
CGAGTGG1001.2732926E-1147.5721631
CGACGGG1300.047.5721631
GGAAGAG48250.047.3456465
CGAGGCG1052.0008883E-1145.3068161
GCCGTCT25400.044.03911250-51
ATGCCGT25750.043.71515748-49
TCGTATG25950.043.6523744-45
TATGCCG25400.043.47605548-49
TGCCGTC25450.043.3926950-51
CGTATGC25550.043.03451546-47
AATCTCG25300.042.98921240-41
CTCGTAT25100.042.85991744-45
TCTCGTA25600.042.67216542-43
ATCTCGT25750.041.8702442-43
Produced by FastQC (version 0.11.2)