##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n01_34310-1.34100000035813.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5775383 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.66082301381571 33.0 33.0 33.0 33.0 33.0 2 32.71296102786603 33.0 33.0 33.0 33.0 33.0 3 32.557488221993246 33.0 33.0 33.0 33.0 33.0 4 36.59976611074971 37.0 37.0 37.0 37.0 37.0 5 36.6618667541183 37.0 37.0 37.0 37.0 37.0 6 36.53500122848995 37.0 37.0 37.0 37.0 37.0 7 36.47880686008183 37.0 37.0 37.0 37.0 37.0 8 36.4082111956904 37.0 37.0 37.0 37.0 37.0 9 36.4170629030144 37.0 37.0 37.0 37.0 37.0 10-11 36.366327393352094 37.0 37.0 37.0 37.0 37.0 12-13 36.33979919600137 37.0 37.0 37.0 37.0 37.0 14-15 38.11914231489063 40.0 37.0 40.0 37.0 40.0 16-17 38.07956017808689 40.0 37.0 40.0 37.0 40.0 18-19 38.122491876296344 40.0 37.0 40.0 37.0 40.0 20-21 38.05224207641294 40.0 37.0 40.0 37.0 40.0 22-23 37.95545187219618 40.0 37.0 40.0 33.0 40.0 24-25 37.90141303182837 40.0 37.0 40.0 33.0 40.0 26-27 37.80265819600189 40.0 37.0 40.0 33.0 40.0 28-29 37.67915530450534 40.0 37.0 40.0 33.0 40.0 30-31 37.65177443643132 40.0 37.0 40.0 33.0 40.0 32-33 37.54443921727788 40.0 37.0 40.0 33.0 40.0 34-35 37.424905326625094 40.0 37.0 40.0 33.0 40.0 36-37 37.29928751045602 40.0 37.0 40.0 33.0 40.0 38-39 37.19897208548767 40.0 37.0 40.0 33.0 40.0 40-41 37.12051564372441 40.0 37.0 40.0 33.0 40.0 42-43 36.95749208320903 37.0 37.0 40.0 33.0 40.0 44-45 36.818600601899476 37.0 37.0 40.0 33.0 40.0 46-47 36.723695727192464 37.0 37.0 40.0 33.0 40.0 48-49 36.52222545240723 37.0 37.0 40.0 33.0 40.0 50-51 36.31874535420421 37.0 37.0 40.0 33.0 40.0 52-53 36.155383028277086 37.0 37.0 40.0 33.0 40.0 54-55 36.010853652476385 37.0 37.0 40.0 33.0 40.0 56-57 35.78651112835287 37.0 37.0 40.0 30.0 40.0 58-59 35.52285069232638 37.0 33.0 40.0 27.0 40.0 60-61 35.23253721874376 37.0 33.0 40.0 27.0 40.0 62-63 34.970527842049606 37.0 33.0 37.0 27.0 40.0 64-65 34.665531792436965 37.0 33.0 37.0 27.0 40.0 66-67 34.30028701126834 37.0 33.0 37.0 27.0 40.0 68-69 33.988577380928675 37.0 33.0 37.0 27.0 38.5 70-71 33.668830534702195 37.0 33.0 37.0 27.0 37.0 72-73 33.31684383529196 37.0 33.0 37.0 27.0 37.0 74-75 32.92429800759534 37.0 33.0 37.0 27.0 37.0 76-77 30.28564685666734 33.0 30.0 33.0 22.0 37.0 78-79 32.72395519050425 33.0 33.0 37.0 27.0 37.0 80-81 33.19566459921359 37.0 33.0 37.0 27.0 37.0 82-83 33.11715387533606 37.0 33.0 37.0 27.0 37.0 84-85 32.9609022466562 37.0 33.0 37.0 27.0 37.0 86-87 32.797327467286586 33.0 33.0 37.0 27.0 37.0 88-89 32.69980873995716 33.0 33.0 37.0 27.0 37.0 90-91 32.57538634234301 33.0 33.0 37.0 27.0 37.0 92-93 32.36498843453326 33.0 33.0 37.0 22.0 37.0 94-95 32.2099514439129 33.0 33.0 37.0 22.0 37.0 96-97 32.06307321955964 33.0 33.0 37.0 22.0 37.0 98-99 31.87034920454626 33.0 33.0 37.0 22.0 37.0 100-101 30.127644954455835 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 0.1919902003763454 2306 2 0.159724743962947 2306 3 0.3669504108583084 2306 4 0.24613881511582036 2306 5 0.17703634160366732 2306 6 0.18482339392974723 2306 7 0.24977330336324854 2306 8 0.2786544622453846 2306 9 0.20568957751662253 2306 10-11 0.21676039236026412 2306 12-13 0.24498386728122767 2306 14-15 0.45765790327246236 2306 16-17 0.489382873528065 2306 18-19 0.450636850561267 2306 20-21 0.42841990060711055 2306 22-23 0.45978421774595546 2306 24-25 0.4825597484520756 2306 26-27 0.5241398072844987 2306 28-29 0.554882619832405 2306 30-31 0.4422363257751414 2306 32-33 0.4760040216118 2306 34-35 0.49078086289352996 2306 36-37 0.50813657389957 2306 38-39 0.6242969705385448 2306 40-41 0.5748619476607857 2306 42-43 0.6000885899659778 2306 44-45 0.4912264405500011 2306 46-47 0.532530501073424 2306 48-49 0.6307970316449953 2306 50-51 0.5868277692911192 2306 52-53 0.6118108282174788 2306 54-55 0.6403907350999063 2306 56-57 0.7161105082806571 2306 58-59 0.6552820382283429 2306 60-61 0.6727864901264127 2306 62-63 0.7470354605989726 2306 64-65 0.6648574230429247 2306 66-67 0.4864296003640334 2306 68-69 0.5807923262825909 2306 70-71 0.6248208391690468 2306 72-73 0.6246852065235018 2306 74-75 0.5137812583389447 2306 76-77 0.15374900037787498 2306 78-79 0.4087634089406791 2306 80-81 0.555753877699523 2306 82-83 0.49521996096594023 2306 84-85 0.5918886355070612 2306 86-87 0.5831341935352086 2306 88-89 0.7012625538814348 2306 90-91 0.6862505458027499 2306 92-93 0.6005865895684863 2306 94-95 0.5437980233857971 2306 96-97 0.6476546694120984 2306 98-99 0.5152625107885243 2306 100-101 0.4862494498072216 2307 1 0.12737921039952482 2307 2 0.12859265367318073 2307 3 0.31728917612323215 2307 4 0.25139300082692984 2307 5 0.17066090698882164 2307 6 0.1805428214138871 2307 7 0.1822092540045901 2307 8 0.17641466284582918 2307 9 0.18172667953238175 2307 10-11 0.1833536420022952 2307 12-13 0.17939578322047822 2307 14-15 0.3501976626446677 2307 16-17 0.3148878512276525 2307 18-19 0.32274192345884956 2307 20-21 0.2968580154981808 2307 22-23 0.38144874534420126 2307 24-25 0.3456797846257089 2307 26-27 0.4032172728224026 2307 28-29 0.42472779047908915 2307 30-31 0.36626354252730664 2307 32-33 0.4046165180942296 2307 34-35 0.3122659032134436 2307 36-37 0.3528740081647115 2307 38-39 0.3438158208165447 2307 40-41 0.3684551735407098 2307 42-43 0.41857422617951556 2307 44-45 0.29138079638739356 2307 46-47 0.37707294803690417 2307 48-49 0.3136735448462886 2307 50-51 0.2585038471109087 2307 52-53 0.3133570159568251 2307 54-55 0.4079032155242217 2307 56-57 0.41621638483200485 2307 58-59 0.2528789074767914 2307 60-61 0.352372930632356 2307 62-63 0.45851391829946664 2307 64-65 0.29260546239641627 2307 66-67 0.15157440907309194 2307 68-69 0.26304141649048063 2307 70-71 0.1615600082400661 2307 72-73 0.25008059664165216 2307 74-75 0.07678755863239672 2307 76-77 -0.15026698432324181 2307 78-79 0.2459981472431565 2307 80-81 0.2782127268348731 2307 82-83 0.2880490023251667 2307 84-85 0.2994789405474094 2307 86-87 0.3071265898934712 2307 88-89 0.36016975390693773 2307 90-91 0.2453351215592363 2307 92-93 0.2968000846114407 2307 94-95 0.2782847237731687 2307 96-97 0.3554144735939815 2307 98-99 0.14933880576973024 2307 100-101 0.08751931827393022 2201 1 0.15234080085873103 2201 2 0.1240462084981715 2201 3 0.30022011823871964 2201 4 0.18689846868768 2201 5 0.11189165955293845 2201 6 0.1815865093912521 2201 7 0.1572939330763674 2201 8 0.2089796145612155 2201 9 0.1782555636978529 2201 10-11 0.203206768836786 2201 12-13 0.20541447081093622 2201 14-15 0.335439351778291 2201 16-17 0.30890657358650486 2201 18-19 0.3039688695192808 2201 20-21 0.33649459986762054 2201 22-23 0.36542114941150317 2201 24-25 0.24900704620935699 2201 26-27 0.298867475804137 2201 28-29 0.3870925885775165 2201 30-31 0.3876048469401212 2201 32-33 0.3587127443025224 2201 34-35 0.3747297435484711 2201 36-37 0.3796649676177637 2201 38-39 0.34816030717386326 2201 40-41 0.3081963440103834 2201 42-43 0.31660410347038237 2201 44-45 0.28802620239007126 2201 46-47 0.4090459359589218 2201 48-49 0.2867174619982791 2201 50-51 0.344003765807976 2201 52-53 0.28779211957407114 2201 54-55 0.4453077057781556 2201 56-57 0.44004169525126713 2201 58-59 0.43387410307764895 2201 60-61 0.4366288720535394 2201 62-63 0.3204776766955604 2201 64-65 0.3687282749818124 2201 66-67 0.42458790917602585 2201 68-69 0.3763672110173033 2201 70-71 0.36786325927617725 2201 72-73 0.3713840427200026 2201 74-75 0.34634833411185895 2201 76-77 0.09220033073166078 2201 78-79 0.4491713148584253 2201 80-81 0.5356639498451159 2201 82-83 0.5055101799096491 2201 84-85 0.5389913126913868 2201 86-87 0.5362647819833697 2201 88-89 0.5341810063600647 2201 90-91 0.4842089953758091 2201 92-93 0.48464722534522764 2201 94-95 0.5332167483730785 2201 96-97 0.5892294128542233 2201 98-99 0.4607038374948331 2201 100-101 0.3151495900589296 1101 1 0.12796315722535923 1101 2 0.0927614850643863 1101 3 0.31848275437008766 1101 4 0.20086984087636495 1101 5 0.14838203876288247 1101 6 0.1275860208885078 1101 7 0.16844362039584837 1101 8 0.16405035053324468 1101 9 0.15117661622740997 1101 10-11 0.15207129074379822 1101 12-13 0.17997413986971367 1101 14-15 0.21472945261920984 1101 16-17 0.1890615375109661 1101 18-19 0.17027449001467687 1101 20-21 0.24296619498414884 1101 22-23 0.24589053851168785 1101 24-25 0.3125706543060929 1101 26-27 0.21893737821989845 1101 28-29 0.18444313969389725 1101 30-31 0.16850933302360716 1101 32-33 0.2398359904419891 1101 34-35 0.24372212910894575 1101 36-37 0.31388826764762 1101 38-39 0.3663669205704778 1101 40-41 0.39067625142748597 1101 42-43 0.3133527494260875 1101 44-45 0.2336743602307294 1101 46-47 0.36934888519727593 1101 48-49 0.3300843895874408 1101 50-51 0.2971527668974048 1101 52-53 0.2618762738634004 1101 54-55 0.2743191468224637 1101 56-57 0.3932567992907181 1101 58-59 0.376315395516329 1101 60-61 0.3367315157838391 1101 62-63 0.414282124396685 1101 64-65 0.32748332962821536 1101 66-67 0.1605019714640008 1101 68-69 0.14143742359352984 1101 70-71 0.15273256540844216 1101 72-73 0.28773579958521367 1101 74-75 0.20670381677970084 1101 76-77 -0.043727107226548156 1101 78-79 0.2078716067969637 1101 80-81 0.27723756837099955 1101 82-83 0.3858257488020982 1101 84-85 0.36714457848727733 1101 86-87 0.4092923552490859 1101 88-89 0.5365802914401812 1101 90-91 0.40180459264794877 1101 92-93 0.35510502219787554 1101 94-95 0.37955462555729014 1101 96-97 0.41328652502763674 1101 98-99 0.3535657850699607 1101 100-101 0.27960868958555096 2304 1 0.16087884322840296 2304 2 0.10798071267240061 2304 3 0.31208529337611424 2304 4 0.20413113522966597 2304 5 0.12706782975754294 2304 6 0.20348859652155227 2304 7 0.19934315546051806 2304 8 0.11126021284018606 2304 9 0.10003898801093669 2304 10-11 0.09884333749320007 2304 12-13 0.09954313273182436 2304 14-15 0.265896697899322 2304 16-17 0.22996988759517478 2304 18-19 0.307145368379679 2304 20-21 0.24678793531084864 2304 22-23 0.319309837270751 2304 24-25 0.3062929321211598 2304 26-27 0.27447073071377304 2304 28-29 0.3496143929162798 2304 30-31 0.318157082740143 2304 32-33 0.3006631301872176 2304 34-35 0.3617108150401407 2304 36-37 0.3777094471778426 2304 38-39 0.33507977875731143 2304 40-41 0.3460957797625994 2304 42-43 0.3396869630930439 2304 44-45 0.3987518991053136 2304 46-47 0.39889006605940125 2304 48-49 0.29812363482549387 2304 50-51 0.35715299472097684 2304 52-53 0.3733047801995255 2304 54-55 0.402164531730385 2304 56-57 0.45782756216669185 2304 58-59 0.32389599686539583 2304 60-61 0.3734255680115197 2304 62-63 0.2615809255189845 2304 64-65 0.2832740789399182 2304 66-67 0.06463889693239366 2304 68-69 0.19778603395739935 2304 70-71 0.2813551791756339 2304 72-73 0.20597224364488653 2304 74-75 0.14084624506347154 2304 76-77 -0.017209451776398765 2304 78-79 0.2700450523830611 2304 80-81 0.230781889693354 2304 82-83 0.28377660725882237 2304 84-85 0.2768499062290033 2304 86-87 0.2869300702137636 2304 88-89 0.419150693303429 2304 90-91 0.3434917050192894 2304 92-93 0.40658815361526734 2304 94-95 0.42858063599861396 2304 96-97 0.3067380432964555 2304 98-99 0.3492449358839025 2304 100-101 0.24355837036968353 2202 1 0.1221691658090478 2202 2 0.08366180579681526 2202 3 0.2934496512523026 2202 4 0.18014897056912815 2202 5 0.11193098723971673 2202 6 0.09228779857848224 2202 7 0.09625696505079873 2202 8 0.1561301931515615 2202 9 0.10210371370400395 2202 10-11 0.13435124433638634 2202 12-13 0.14415719695841034 2202 14-15 0.0637065137349282 2202 16-17 -0.00577689825637151 2202 18-19 0.07373718122459394 2202 20-21 0.16529345976285015 2202 22-23 0.19535920719886946 2202 24-25 0.1704213476850569 2202 26-27 0.18591780639559374 2202 28-29 0.22043883944125753 2202 30-31 0.15973290832847198 2202 32-33 0.2578846509742476 2202 34-35 0.15919988591558365 2202 36-37 0.24632827218747622 2202 38-39 0.19819288854767336 2202 40-41 0.24577255522793706 2202 42-43 0.16299292379019192 2202 44-45 0.2038273281616938 2202 46-47 0.23540190062858812 2202 48-49 0.2718481237032009 2202 50-51 0.3292329458013725 2202 52-53 0.31209906310057534 2202 54-55 0.21165820261645507 2202 56-57 0.21718257684617726 2202 58-59 0.3436769824482653 2202 60-61 0.3135668997589889 2202 62-63 0.1299702699074814 2202 64-65 0.18164885782785944 2202 66-67 0.10423886157846596 2202 68-69 0.04464485298405663 2202 70-71 0.1535997620052285 2202 72-73 0.13830233580107176 2202 74-75 0.06060893378608512 2202 76-77 -0.15357933070517404 2202 78-79 0.1403001566071751 2202 80-81 0.15316266915160526 2202 82-83 0.1947893374743188 2202 84-85 0.21768147953103067 2202 86-87 0.3342851040899504 2202 88-89 0.3644803347369603 2202 90-91 0.374861281999884 2202 92-93 0.3931519592662269 2202 94-95 0.24599762724292162 2202 96-97 0.3346964656311826 2202 98-99 0.27555340135123174 2202 100-101 0.1097355743614763 1102 1 0.1058378964592066 1102 2 0.06215152479385466 1102 3 0.2566770234940492 1102 4 0.1617560803958895 1102 5 0.04015162778213721 1102 6 0.07777852278196917 1102 7 0.028007426718012596 1102 8 0.08540576981864234 1102 9 0.01399285747429957 1102 10-11 0.06114088707115428 1102 12-13 0.14059546090772557 1102 14-15 0.12940518907598175 1102 16-17 0.10223886513955449 1102 18-19 0.1118468434873563 1102 20-21 0.04308976824448507 1102 22-23 0.10717631496367375 1102 24-25 0.10763536417468345 1102 26-27 0.014405611255149608 1102 28-29 0.1324566663152993 1102 30-31 0.09861910394801754 1102 32-33 0.09146586195237916 1102 34-35 0.09625156080483066 1102 36-37 0.10308864567549136 1102 38-39 0.11915462702418722 1102 40-41 0.13326849953070052 1102 42-43 0.06764334922145565 1102 44-45 0.1453303581844665 1102 46-47 0.21288578776042755 1102 48-49 0.15616648608088468 1102 50-51 0.1119866239776286 1102 52-53 0.10799185472415473 1102 54-55 0.13408467320468986 1102 56-57 0.17328153055209583 1102 58-59 0.04333130059080759 1102 60-61 0.0028520130001155053 1102 62-63 0.1476846078206009 1102 64-65 0.1071408290634892 1102 66-67 -0.007639214307801012 1102 68-69 -0.05101572154525513 1102 70-71 -0.10991334737276048 1102 72-73 -0.1431338472344379 1102 74-75 -0.06374340547942836 1102 76-77 -0.23247687469622136 1102 78-79 0.04872967692811159 1102 80-81 0.11270674813687265 1102 82-83 0.05684582613504574 1102 84-85 0.07594261260944535 1102 86-87 0.194088027527485 1102 88-89 0.22999951704775157 1102 90-91 0.1953497168191447 1102 92-93 0.15582885589218876 1102 94-95 0.09891742601969611 1102 96-97 0.06153623602565972 1102 98-99 -0.06288033765289924 1102 100-101 -0.19108509786844152 2305 1 0.06083959430490893 2305 2 0.03875366877444719 2305 3 0.23357898600714577 2305 4 0.11407674476127738 2305 5 0.07264186380452742 2305 6 0.051870782680111915 2305 7 0.07759642931828381 2305 8 0.07114710739350016 2305 9 0.04256098803699615 2305 10-11 0.03637368232569571 2305 12-13 0.0208722261349763 2305 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96-97 0.15051908261047942 1213 98-99 0.07416659356867683 1213 100-101 0.3922886094737592 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 14.0 9 60.0 10 42.0 11 49.0 12 72.0 13 179.0 14 554.0 15 1257.0 16 2483.0 17 4298.0 18 6407.0 19 8406.0 20 11528.0 21 14671.0 22 19210.0 23 24271.0 24 30560.0 25 38880.0 26 49206.0 27 63975.0 28 84729.0 29 110321.0 30 126226.0 31 152418.0 32 206393.0 33 292929.0 34 448032.0 35 766360.0 36 1382973.0 37 1667792.0 38 261027.0 39 60.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.970480667306745 24.70750872704468 22.898884214732472 15.423126390916103 2 31.67040177248851 21.98529517436333 32.49434712814717 13.849955925000993 3 39.46200259342197 19.018000717182122 24.737727259898524 16.78226942949738 4 31.24546579776241 18.25171726009405 32.75082527026833 17.75199167187521 5 34.31427837772837 16.822815041011133 31.33866619754915 17.524240383711348 6 28.077202845248532 17.78952149147511 36.354108463456015 17.779167199820343 7 34.47800777887804 18.687764949960894 27.463252220675233 19.37097505048583 8 30.084758015182718 26.02107946780326 24.858143607099304 19.03601890991472 9 31.65793506681721 23.617048427783924 24.81594727137577 19.909069234023093 10-11 26.14455151688458 27.99539285208134 28.372918219055443 17.487137411978637 12-13 25.841463965753945 26.25923726936803 27.94763670137087 19.951662063507154 14-15 25.447688923834143 26.745784651857722 27.713529994460973 20.09299642984716 16-17 24.850741500606972 27.06627421938943 28.553534891105926 19.52944938889767 18-19 23.39185124172717 27.19823949338079 29.21130944908762 20.198599815804425 20-21 23.360242948389743 27.867528439239443 29.013365866817836 19.758862745552978 22-23 23.225810305567613 28.467964808567675 28.43033959825695 19.875885287607765 24-25 23.469949958297136 26.759575945006592 29.471811653010715 20.298662443685554 26-27 22.620001586042275 26.67288501435922 29.361330557874787 21.345782841723715 28-29 22.404687557896683 27.89874733653613 28.333723495655867 21.362841609911325 30-31 20.94079301753667 28.440425509442402 28.837974901404806 21.780806571616115 32-33 20.995533975841948 28.10093287319646 28.850337717862175 22.053195433099415 34-35 21.98136469910307 28.096249201135233 29.03829062072593 20.884095479035764 36-37 21.31454684510177 29.328672813164847 29.331270071395167 20.02551027033822 38-39 21.237234749704218 28.56430098485857 28.838604849197807 21.359859416239406 40-41 21.714387773441736 27.832584347093743 27.769514707476343 22.683513171988178 42-43 22.37209775064637 28.41708413068788 28.32771289279884 20.883105225866906 44-45 21.25994688645085 28.622030218882365 28.261533366953156 21.85648952771363 46-47 22.046680932877553 28.347570540819948 28.43465866761486 21.17108985868763 48-49 22.106078173472714 28.302283089842074 28.723172153271705 20.868466583413504 50-51 21.772328277138172 28.282480046108084 27.587387046846505 22.357804629907243 52-53 21.08575446280964 28.457551415804154 27.83153255619274 22.625161565193466 54-55 22.017988325069155 28.44641238256433 28.317616369872777 21.217982922493736 56-57 21.936314395172214 28.26198177727225 27.56956210681144 22.232141720744096 58-59 21.34564212225646 27.925751035538603 28.209295130645806 22.51931171155913 60-61 21.081594374750654 27.86953273915067 28.924422832904828 22.124450053193847 62-63 20.824559849681773 27.91212343714679 28.810306526277074 22.453010186894357 64-65 21.88687544211603 28.423534382665327 28.293339059376237 21.396251115842407 66-67 22.164844808209974 28.731822331261824 27.72430576879413 21.379027091734066 68-69 21.74156228215642 27.9010984728759 28.658364977030352 21.69897426793732 70-71 21.389192962450856 29.27403571251977 28.40385468192414 20.932916643105234 72-73 22.345010496483724 29.52704211660514 27.705071105310086 20.42287628160105 74-75 23.386191291426258 28.49752293268144 27.442814426481625 20.673471349410676 76-77 23.38088771449567 27.219117377338325 27.90527664678831 21.49471826137769 78-79 23.039444549696718 27.423678921379324 28.622417648947458 20.914458879976504 80-81 22.170483561676924 28.450973420128918 28.24200152428189 21.13654149391227 82-83 21.33624503447256 28.42239163280706 28.575127811143908 21.666235521576475 84-85 21.548085566027233 27.957736808813223 28.749903247574178 21.744274377585366 86-87 20.775292655244346 28.563538026046047 29.243159024559866 21.418010294149738 88-89 20.709431993927026 29.117438558723435 29.139795718595224 21.03333372875431 90-91 21.436822034922027 28.23620988063026 28.38699699490885 21.939971089538865 92-93 21.34060406651072 28.587950865744844 27.627692092670674 22.443752975073764 94-95 21.0747130945744 29.59144369504295 27.144553434127573 22.189289776255077 96-97 21.087584127244195 30.42800955966416 26.41498911556826 22.069417197523382 98-99 21.330020844362526 29.052195083972844 27.324760433591145 22.293023638073485 100-101 21.964806458730443 29.054952066274865 27.0618152294054 21.91842624558929 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 204.0 1 139.5 2 170.5 3 335.0 4 331.5 5 207.5 6 176.0 7 198.5 8 211.0 9 204.5 10 185.0 11 202.5 12 220.5 13 221.5 14 212.0 15 216.0 16 234.0 17 251.5 18 280.5 19 320.0 20 359.5 21 425.5 22 575.0 23 751.5 24 1050.5 25 1485.0 26 2022.0 27 2773.5 28 3804.0 29 5022.5 30 6684.0 31 9594.0 32 15951.0 33 27923.0 34 47579.5 35 81391.0 36 127799.0 37 194113.5 38 279463.0 39 332621.5 40 351749.0 41 344400.5 42 354769.0 43 399262.5 44 396912.0 45 377640.0 46 391777.0 47 376575.5 48 311535.0 49 253253.0 50 205292.0 51 169531.0 52 151894.5 53 138535.5 54 110881.0 55 70332.5 56 45130.5 57 35906.0 58 31241.0 59 26942.0 60 21970.0 61 17413.5 62 12643.5 63 8457.0 64 5950.5 65 4457.5 66 3586.0 67 3011.5 68 2416.5 69 1797.0 70 1267.0 71 892.0 72 683.5 73 522.0 74 329.0 75 189.0 76 137.0 77 98.0 78 60.5 79 38.5 80 28.5 81 18.5 82 15.0 83 15.0 84 8.5 85 2.5 86 2.5 87 3.0 88 2.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16274245361736184 2 0.0 3 3.4629737975819095E-5 4 0.02102025095132219 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.644912553851407E-4 12-13 3.4629737975819095E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.7314868987909548E-5 28-29 1.5583382089118592E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.001073521877250392 38-39 6.233352835647437E-4 40-41 3.6361224874610046E-4 42-43 6.060204145768341E-4 44-45 3.5495481425214573E-4 46-47 0.0034370014941000446 48-49 0.0039304752602554675 50-51 0.0044412638953987985 52-53 0.004112281384628517 54-55 0.0062246954011534825 56-57 0.009722298936711211 58-59 0.016838710090742035 60-61 0.004830848447626763 62-63 0.002917555424462759 64-65 0.010354291654769908 66-67 0.023098035229871337 68-69 0.025245078984372116 70-71 0.02544419997773308 72-73 0.022621876332703822 74-75 0.024552484224855738 76-77 0.02479489239068647 78-79 0.015531437482154862 80-81 0.0035408907080275026 82-83 0.0011254664842141205 84-85 0.0057831662419617885 86-87 0.014561804818831927 88-89 0.016388523497056384 90-91 0.023349100830196024 92-93 0.023660768471978396 94-95 0.024968041080565568 96-97 0.026664898241380703 98-99 0.03254329626277599 100-101 0.02370405564444817 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5775383.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.272336091282664 #Duplication Level Percentage of deduplicated Percentage of total 1 82.36478146356657 24.933743465501358 2 7.447439360720864 4.5090277469437865 3 2.997444333983195 2.7221892687975062 4 1.4885791690920862 1.8025107562095168 5 0.9041751185056228 1.3685746536388772 6 0.6569934751372467 1.1933236373480693 7 0.4998990791860823 1.0593179055790667 8 0.351940872204922 0.8523257894117249 9 0.27491685066914007 0.7490137770551857 >10 2.328985926995306 14.213503861696038 >50 0.3281167373924923 6.943428409852458 >100 0.2992162331295338 18.394318743501195 >500 0.03643035789673482 7.7116892620967725 >1k 0.020332801308482997 10.589415719655852 >5k 4.6045173790360016E-4 0.9293187414233763 >10k+ 2.8776847388138757E-4 2.0282982612891165 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT 43939 0.7607980284597576 TruSeq Adapter, Index 7 (97% over 38bp) TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 24271 0.4202491852055526 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 24184 0.41874279160360445 No Hit TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT 12948 0.22419292365545282 No Hit GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 11074 0.19174485917211032 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 8124 0.14066599565777715 No Hit TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 7961 0.1378436720127479 No Hit GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 7586 0.13135059614228184 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 6914 0.1197150041824066 No Hit TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 6237 0.10799283787759184 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 6095 0.10553412648130868 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 6.233352835647437E-4 0.0 0.0 2 6.406501525526532E-4 0.0 0.0 3 6.925947595163819E-4 0.0 0.0 4 7.099096285042914E-4 0.0 0.0 5 8.137988424317487E-4 0.0 0.0 6 9.523177943350251E-4 0.0 0.0 7 0.001073521877250392 0.0 0.0 8 0.0012293556981415779 0.0 0.0 9 0.0014025043880206733 0.0 0.0 10-11 0.0023894519203315175 0.0 0.0 12-13 0.0033331122801725874 0.0 0.0 14-15 0.004475893633374617 0.0 0.0 16-17 0.0057052493315161955 0.0 0.0 18-19 0.007540625444234608 0.0 0.0 20-21 0.009895447626590306 0.0 0.0 22-23 0.01240610362983719 0.0 0.0 24-25 0.015479492875191134 0.0 0.0 26-27 0.018379733430665984 0.0 0.0 28-29 0.021401178069056198 0.0 0.0 30-31 0.032621213173221586 0.0 0.0 32-33 0.0370797919376083 0.0 0.0 34-35 0.04107086923932145 0.0 0.0 36-37 0.04554676287269606 1.7314868987909548E-5 0.0 38-39 0.0504208984927926 1.7314868987909548E-5 0.0 40-41 0.05655901954900654 1.7314868987909548E-5 0.0 42-43 0.06300015081250888 1.7314868987909548E-5 0.0 44-45 0.06978757945576944 1.7314868987909548E-5 0.0 46-47 0.0769732500857519 1.7314868987909548E-5 0.0 48-49 0.08588175018003136 1.7314868987909548E-5 0.0 50-51 0.09604557827593425 1.7314868987909548E-5 1.7314868987909548E-5 52-53 0.10685005652438981 1.7314868987909548E-5 1.7314868987909548E-5 54-55 0.11954185549252751 1.7314868987909548E-5 1.7314868987909548E-5 56-57 0.1352031544920917 1.7314868987909548E-5 1.7314868987909548E-5 58-59 0.15147047390623272 1.7314868987909548E-5 1.7314868987909548E-5 60-61 0.1696770586470196 1.7314868987909548E-5 1.7314868987909548E-5 62-63 0.19156305304773727 1.7314868987909548E-5 1.7314868987909548E-5 64-65 0.21587312910676226 1.7314868987909548E-5 2.597230348186432E-5 66-67 0.24218307253389082 1.7314868987909548E-5 3.4629737975819095E-5 68-69 0.27207719384151663 1.7314868987909548E-5 3.4629737975819095E-5 70-71 0.30485424083562945 1.7314868987909548E-5 3.4629737975819095E-5 72-73 0.3427045444431997 1.7314868987909548E-5 3.4629737975819095E-5 74-75 0.3854376411053605 1.7314868987909548E-5 3.4629737975819095E-5 76-77 0.43194537920688547 1.7314868987909548E-5 4.328717246977386E-5 78-79 0.4851626290412255 1.7314868987909548E-5 5.1944606963728636E-5 80-81 0.5434791077925047 1.7314868987909548E-5 5.1944606963728636E-5 82-83 0.61102441171434 1.7314868987909548E-5 8.657434493954774E-5 84-85 0.6863527492462405 1.7314868987909548E-5 8.657434493954774E-5 86-87 0.7712908390664308 1.7314868987909548E-5 8.657434493954774E-5 88-89 0.8624449668532805 1.7314868987909548E-5 8.657434493954774E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGTGTT 62735 0.0 46.66475 1 GCCGTCT 5745 0.0 42.411785 50-51 CGGAAGA 11505 0.0 42.239586 4 CGAGGTG 520 0.0 42.088787 1 TGCCGTC 5725 0.0 42.062172 50-51 ATGCCGT 5770 0.0 42.020416 48-49 TATGCCG 5755 0.0 41.758568 48-49 TCGTATG 5840 0.0 41.719337 44-45 GAAGAGC 11735 0.0 41.56284 6 TCGGAAG 11615 0.0 41.43057 3 CGTATGC 5795 0.0 41.347034 46-47 TCTCGTA 5855 0.0 41.165962 42-43 CTCGTAT 5830 0.0 40.8948 44-45 CATCTCG 5865 0.0 40.568897 40-41 ATTACGG 275 0.0 39.793034 1 ATCTCGT 5995 0.0 39.372803 42-43 GATCGGA 12280 0.0 39.248493 1 CTGCTTG 6250 0.0 39.104153 58-59 CTTGAAA 5985 0.0 38.617416 62-63 CGTCTTC 6470 0.0 37.662727 52-53 >>END_MODULE