FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n01_34310-1.34100000035813.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n01_34310-1.34100000035813.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5775383
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT439390.7607980284597576TruSeq Adapter, Index 7 (97% over 38bp)
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA242710.4202491852055526No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC241840.41874279160360445No Hit
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT129480.22419292365545282No Hit
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC110740.19174485917211032No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC81240.14066599565777715No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT79610.1378436720127479No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC75860.13135059614228184No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA69140.1197150041824066No Hit
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT62370.10799283787759184No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG60950.10553412648130868No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTGTT627350.046.664751
GCCGTCT57450.042.41178550-51
CGGAAGA115050.042.2395864
CGAGGTG5200.042.0887871
TGCCGTC57250.042.06217250-51
ATGCCGT57700.042.02041648-49
TATGCCG57550.041.75856848-49
TCGTATG58400.041.71933744-45
GAAGAGC117350.041.562846
TCGGAAG116150.041.430573
CGTATGC57950.041.34703446-47
TCTCGTA58550.041.16596242-43
CTCGTAT58300.040.894844-45
CATCTCG58650.040.56889740-41
ATTACGG2750.039.7930341
ATCTCGT59950.039.37280342-43
GATCGGA122800.039.2484931
CTGCTTG62500.039.10415358-59
CTTGAAA59850.038.61741662-63
CGTCTTC64700.037.66272752-53
Produced by FastQC (version 0.11.2)