FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n01_34302-1.341000000358ea.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n01_34302-1.341000000358ea.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8355919
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT4859055.815099452256538TruSeq Adapter, Index 7 (97% over 35bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC162290.1942216050682157No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA141920.1698436760815896No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG133810.16013798123222592No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA113310.1356044739064608No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC106860.12788539477225663No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC102540.12271540688702225No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA98540.11792838106736075No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG89020.1065352596165664No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG624300.082.479993
CGGAAGA628700.082.477024
GATCGGA631400.081.809341
GAAGAGC640750.080.8434146
ATCGGAA637500.080.824332
GGAAGAG671450.077.288565
AGAGCAC924250.056.0878
GAGCACA926500.056.048199
AAGAGCA929550.055.6650247
TTGTGTT941550.047.279361
GCCGTCT561700.045.68355650-51
ATGCCGT561550.045.56383548-49
TCGTATG565300.045.54277444-45
TCTCGTA561350.045.4401942-43
CGTCTTC565700.045.39669852-53
TATGCCG561700.045.3867848-49
CGTATGC563050.045.35360746-47
TGCCGTC563250.045.3385950-51
CGTATCT557600.045.3348938-39
CTCGTAT562150.045.2614644-45
Produced by FastQC (version 0.11.2)