##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n01_34298-1.341000000357c1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5319197 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.65487516254803 33.0 33.0 33.0 33.0 33.0 2 32.7060095724975 33.0 33.0 33.0 33.0 33.0 3 32.53958445231489 33.0 33.0 33.0 33.0 33.0 4 36.59035113758712 37.0 37.0 37.0 37.0 37.0 5 36.65521938743762 37.0 37.0 37.0 37.0 37.0 6 36.5296085104575 37.0 37.0 37.0 37.0 37.0 7 36.452999766694106 37.0 37.0 37.0 37.0 37.0 8 36.38560782764767 37.0 37.0 37.0 37.0 37.0 9 36.40796232965239 37.0 37.0 37.0 37.0 37.0 10-11 36.352697973021115 37.0 37.0 37.0 37.0 37.0 12-13 36.33144974702009 37.0 37.0 37.0 37.0 37.0 14-15 38.12556622738357 40.0 37.0 40.0 37.0 40.0 16-17 38.107767300214675 40.0 37.0 40.0 37.0 40.0 18-19 38.110537361184406 40.0 37.0 40.0 37.0 40.0 20-21 38.05569534273688 40.0 37.0 40.0 37.0 40.0 22-23 37.942833570555855 40.0 37.0 40.0 33.0 40.0 24-25 37.8833069164387 40.0 37.0 40.0 33.0 40.0 26-27 37.78350576224193 40.0 37.0 40.0 33.0 40.0 28-29 37.644601149384016 40.0 37.0 40.0 33.0 40.0 30-31 37.59111018072841 40.0 37.0 40.0 33.0 40.0 32-33 37.46840622748133 40.0 37.0 40.0 33.0 40.0 34-35 37.37114173436329 40.0 37.0 40.0 33.0 40.0 36-37 37.28639445013975 40.0 37.0 40.0 33.0 40.0 38-39 37.161294458543274 40.0 37.0 40.0 33.0 40.0 40-41 37.075639236523855 40.0 37.0 40.0 33.0 40.0 42-43 36.899189012928076 37.0 37.0 40.0 33.0 40.0 44-45 36.784360684516855 37.0 37.0 40.0 33.0 40.0 46-47 36.719735798467326 37.0 37.0 40.0 33.0 40.0 48-49 36.488856400693564 37.0 37.0 40.0 33.0 40.0 50-51 36.29920841435277 37.0 37.0 40.0 33.0 40.0 52-53 36.165855109333236 37.0 37.0 40.0 33.0 40.0 54-55 36.01045326954426 37.0 37.0 40.0 33.0 40.0 56-57 35.81753589874562 37.0 37.0 40.0 27.0 40.0 58-59 35.5153198875695 37.0 33.0 40.0 27.0 40.0 60-61 35.22511527585837 37.0 33.0 40.0 27.0 40.0 62-63 34.96981405276021 37.0 33.0 38.5 27.0 40.0 64-65 34.65216497903725 37.0 33.0 37.0 27.0 40.0 66-67 34.24891529680138 37.0 33.0 37.0 27.0 40.0 68-69 33.963208638446744 37.0 33.0 37.0 27.0 40.0 70-71 33.59493951812651 37.0 33.0 37.0 27.0 38.5 72-73 33.12591675021625 37.0 33.0 37.0 27.0 37.0 74-75 32.651678157436166 37.0 33.0 37.0 22.0 37.0 76-77 30.110057965516223 33.0 30.0 35.0 22.0 37.0 78-79 32.38193396484469 33.0 33.0 37.0 24.5 37.0 80-81 32.75779013260836 37.0 33.0 37.0 27.0 37.0 82-83 32.71282291293216 37.0 33.0 37.0 24.5 37.0 84-85 32.56548544827349 37.0 33.0 37.0 22.0 37.0 86-87 32.42737099227571 35.0 33.0 37.0 22.0 37.0 88-89 32.31989546542458 33.0 33.0 37.0 22.0 37.0 90-91 32.170795986687466 33.0 33.0 37.0 22.0 37.0 92-93 31.966992856252553 33.0 33.0 37.0 22.0 37.0 94-95 31.848997884455116 33.0 33.0 37.0 22.0 37.0 96-97 31.706758181733072 33.0 33.0 37.0 18.5 37.0 98-99 31.533797770603343 33.0 33.0 37.0 15.0 37.0 100-101 29.901295721891856 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 0.14505502946744286 2306 2 0.12754668465777286 2306 3 0.34613905874830664 2306 4 0.21680197587279793 2306 5 0.15886362661194653 2306 6 0.15397815000648052 2306 7 0.20190203044712973 2306 8 0.1708745521903694 2306 9 0.19411961281583956 2306 10-11 0.18108541787067622 2306 12-13 0.22073917150643751 2306 14-15 0.39791836104623 2306 16-17 0.3884374592037574 2306 18-19 0.4833996975625112 2306 20-21 0.5036670339608165 2306 22-23 0.5137034060069112 2306 24-25 0.4899988946042413 2306 26-27 0.4863040831642138 2306 28-29 0.46616681095319024 2306 30-31 0.4924505092078988 2306 32-33 0.5276522459934725 2306 34-35 0.5555098298171259 2306 36-37 0.5386595730069743 2306 38-39 0.5036611444579506 2306 40-41 0.5452744774767382 2306 42-43 0.5861940589620005 2306 44-45 0.5383937563348908 2306 46-47 0.6657973766330727 2306 48-49 0.5252114813167879 2306 50-51 0.55650610731238 2306 52-53 0.46859847527982623 2306 54-55 0.6154643701789908 2306 56-57 0.6121116467928829 2306 58-59 0.6979884462728094 2306 60-61 0.6849171384621258 2306 62-63 0.7183749754541751 2306 64-65 0.49370085069657677 2306 66-67 0.3512429383421818 2306 68-69 0.34075749093213403 2306 70-71 0.4726477973807093 2306 72-73 0.5487600361245839 2306 74-75 0.41902201514717063 2306 76-77 0.22638105949738474 2306 78-79 0.503465159255228 2306 80-81 0.5552239903405862 2306 82-83 0.5193008681254909 2306 84-85 0.6563598641193877 2306 86-87 0.5100015372146558 2306 88-89 0.6270832131011943 2306 90-91 0.5977638208052198 2306 92-93 0.5640926606500472 2306 94-95 0.4633890437588981 2306 96-97 0.6011616069706065 2306 98-99 0.4998852828768854 2306 100-101 0.48410498247588407 2307 1 0.12184676651352788 2307 2 0.10018480597822332 2307 3 0.3148542448506291 2307 4 0.23830801347044428 2307 5 0.1460573053100731 2307 6 0.12884244755153418 2307 7 0.18374277343515644 2307 8 0.17039815867784824 2307 9 0.20742419308118087 2307 10-11 0.21911326352245197 2307 12-13 0.20778594170600684 2307 14-15 0.42098367861456865 2307 16-17 0.36381108509682747 2307 18-19 0.40863488781926804 2307 20-21 0.36473983497974416 2307 22-23 0.40143866459320066 2307 24-25 0.3473685081870599 2307 26-27 0.4660236040921859 2307 28-29 0.4775912357551775 2307 30-31 0.41253310594886017 2307 32-33 0.49478765326482943 2307 34-35 0.35535745495582916 2307 36-37 0.4113444317288284 2307 38-39 0.358659859862712 2307 40-41 0.3743660499853121 2307 42-43 0.4164979340665482 2307 44-45 0.38143521322989216 2307 46-47 0.43828872611403824 2307 48-49 0.4385335539428823 2307 50-51 0.2916568695966646 2307 52-53 0.3774501564596804 2307 54-55 0.5324935933541823 2307 56-57 0.44776531444895795 2307 58-59 0.34398884531728413 2307 60-61 0.4733740935887596 2307 62-63 0.40509280727605557 2307 64-65 0.3837743459435714 2307 66-67 0.3798326987514997 2307 68-69 0.42425235528434513 2307 70-71 0.2314791163593739 2307 72-73 0.2867565868496982 2307 74-75 0.2578677653234607 2307 76-77 -0.021747804537774584 2307 78-79 0.3026721266811663 2307 80-81 0.3766271236597021 2307 82-83 0.46543450976167833 2307 84-85 0.4608208260899005 2307 86-87 0.4513025042142189 2307 88-89 0.4478435021624563 2307 90-91 0.4148724428991599 2307 92-93 0.35590637247433676 2307 94-95 0.3047985180587922 2307 96-97 0.39340060181582714 2307 98-99 0.1949935715227049 2307 100-101 0.19400907514166477 2201 1 0.1393362230055999 2201 2 0.12269200373833655 2201 3 0.3255342240717738 2201 4 0.21145750676573982 2201 5 0.15564111189063112 2201 6 0.14662685812761822 2201 7 0.09544848850070764 2201 8 0.19232425286945443 2201 9 0.13660615773951434 2201 10-11 0.2016220461104794 2201 12-13 0.20859156256234712 2201 14-15 0.3713574338643113 2201 16-17 0.33069698629161337 2201 18-19 0.34851620181770215 2201 20-21 0.34427662499885514 2201 22-23 0.3901384013356761 2201 24-25 0.3163829213164817 2201 26-27 0.39208918074943 2201 28-29 0.4040552205408261 2201 30-31 0.44307053870372926 2201 32-33 0.3174718234753513 2201 34-35 0.4372437523575883 2201 36-37 0.4469819287572747 2201 38-39 0.4249642172590171 2201 40-41 0.44659854424030954 2201 42-43 0.48529003105801394 2201 44-45 0.3873254903106371 2201 46-47 0.5145176280924275 2201 48-49 0.535932235013874 2201 50-51 0.5790927060776383 2201 52-53 0.5258446377367036 2201 54-55 0.5076712261495544 2201 56-57 0.6238172439769443 2201 58-59 0.5245953941784123 2201 60-61 0.4853307397407036 2201 62-63 0.42400684797090804 2201 64-65 0.49507460920109736 2201 66-67 0.3684774067469547 2201 68-69 0.45772876335991697 2201 70-71 0.4594196324850017 2201 72-73 0.35939497314269886 2201 74-75 0.3208752404284496 2201 76-77 0.15548350109628828 2201 78-79 0.4713339155997076 2201 80-81 0.4880105903827143 2201 82-83 0.5152254005230148 2201 84-85 0.6165288479605238 2201 86-87 0.5764885013791385 2201 88-89 0.5863275262166212 2201 90-91 0.48977845379925355 2201 92-93 0.48824566652155 2201 94-95 0.4539766086933419 2201 96-97 0.5273506973457245 2201 98-99 0.41641736331153467 2201 100-101 0.3438180153320225 1101 1 0.13392434310794954 1101 2 0.11397228751344812 1101 3 0.3014014517908663 1101 4 0.18265588027349366 1101 5 0.1438854914195815 1101 6 0.1405133071639426 1101 7 0.15444664785649564 1101 8 0.14287086592605647 1101 9 0.1568745274085117 1101 10-11 0.16785631378026977 1101 12-13 0.17551732020758948 1101 14-15 0.3359158455443705 1101 16-17 0.23587966559976792 1101 18-19 0.25319898957871345 1101 20-21 0.2842158749162138 1101 22-23 0.27090786779901777 1101 24-25 0.32078495823272135 1101 26-27 0.2824126795694397 1101 28-29 0.33079878320693723 1101 30-31 0.26259192175737667 1101 32-33 0.2826016852248685 1101 34-35 0.36787736428757967 1101 36-37 0.30001832266541584 1101 38-39 0.40954565856594627 1101 40-41 0.3935465538710403 1101 42-43 0.4367540088566457 1101 44-45 0.3243040548486178 1101 46-47 0.4050653533119757 1101 48-49 0.4289241491917366 1101 50-51 0.32498053935442783 1101 52-53 0.4616705088962547 1101 54-55 0.3853895652122574 1101 56-57 0.4591201900839721 1101 58-59 0.3182258275719221 1101 60-61 0.37232136159880724 1101 62-63 0.43116657871306785 1101 64-65 0.2719130790239106 1101 66-67 0.18102699714228976 1101 68-69 0.2797725358437688 1101 70-71 0.18244825094882344 1101 72-73 0.2998765842993123 1101 74-75 0.25414522152167507 1101 76-77 -0.025545888609386225 1101 78-79 0.28505156797208286 1101 80-81 0.3883469736152563 1101 82-83 0.39174952190216317 1101 84-85 0.35407756811502367 1101 86-87 0.43669182551490593 1101 88-89 0.39251146245313606 1101 90-91 0.4695131384574296 1101 92-93 0.3192870747622436 1101 94-95 0.377709622876953 1101 96-97 0.40366239754227706 1101 98-99 0.28600624699567945 1101 100-101 0.3223997884455443 2304 1 0.10456041687604767 2304 2 0.0555582411192006 2304 3 0.27188434167383235 2304 4 0.14920706546505613 2304 5 0.12119678514720533 2304 6 0.14493927557415986 2304 7 0.10164675929573974 2304 8 0.1534612009155012 2304 9 0.11260005686891361 2304 10-11 0.13934066923360433 2304 12-13 0.09184876000537656 2304 14-15 0.2576864402792225 2304 16-17 0.2727652451717617 2304 18-19 0.28312265910363976 2304 20-21 0.20518802457119278 2304 22-23 0.31489145575510236 2304 24-25 0.34732384092804125 2304 26-27 0.2980050003593959 2304 28-29 0.29445342702735644 2304 30-31 0.23065163912905007 2304 32-33 0.3121865740268035 2304 34-35 0.34645289841381555 2304 36-37 0.29384365516866495 2304 38-39 0.3499379454552951 2304 40-41 0.3778356485578911 2304 42-43 0.418561055766169 2304 44-45 0.4151856015099682 2304 46-47 0.4154842242322374 2304 48-49 0.3809674500114397 2304 50-51 0.38489092362598853 2304 52-53 0.2924653385118674 2304 54-55 0.4391702048068211 2304 56-57 0.3930655001010166 2304 58-59 0.29599550085927007 2304 60-61 0.3740933448162025 2304 62-63 0.3930739434859092 2304 64-65 0.23573970534042843 2304 66-67 0.2137579033660728 2304 68-69 0.33575002078797667 2304 70-71 0.3145634688526755 2304 72-73 0.21194434585358835 2304 74-75 0.14011379403090274 2304 76-77 -0.03130527360178448 2304 78-79 0.22016291029659385 2304 80-81 0.3744734634727962 2304 82-83 0.4443238722118039 2304 84-85 0.46670354022185023 2304 86-87 0.46869287679454175 2304 88-89 0.5438893059684418 2304 90-91 0.45934056540544077 2304 92-93 0.572651417538907 2304 94-95 0.5317302265152257 2304 96-97 0.4092226840565587 2304 98-99 0.5261725222875917 2304 100-101 0.3329159433046236 2202 1 0.10869293719120066 2202 2 0.09780442055759409 2202 3 0.2944276908250174 2202 4 0.20372708816226748 2202 5 0.07119872489728607 2202 6 0.09320068396914394 2202 7 0.06211512154555976 2202 8 0.11108085898231934 2202 9 0.07981043619450645 2202 10-11 0.09631023391531102 2202 12-13 0.06627002171262575 2202 14-15 -0.053718437574069355 2202 16-17 -0.017524238014871685 2202 18-19 -0.025853331906155574 2202 20-21 0.13290703194778075 2202 22-23 0.1993414598690748 2202 24-25 0.13848653139125133 2202 26-27 0.09876770582901884 2202 28-29 0.13911092897883037 2202 30-31 0.20454985042367468 2202 32-33 0.16862969906223668 2202 34-35 0.12745771453617039 2202 36-37 0.05947650609397215 2202 38-39 0.17178351747480747 2202 40-41 0.21895898980511674 2202 42-43 0.24960583926660718 2202 44-45 0.20930913929012007 2202 46-47 0.2402000721609454 2202 48-49 0.31739578605118623 2202 50-51 0.24716459635732235 2202 52-53 0.2334001189982402 2202 54-55 0.23959765325103177 2202 56-57 0.20671829250981943 2202 58-59 0.27101228715807935 2202 60-61 0.24033355067697926 2202 62-63 0.19818880866475297 2202 64-65 0.16257397351962055 2202 66-67 0.14909980883160756 2202 68-69 0.16652537087023234 2202 70-71 0.24052229158210991 2202 72-73 0.16395139034377593 2202 74-75 -0.005359703227661328 2202 76-77 -0.21122466099886594 2202 78-79 0.09587242185046563 2202 80-81 0.1725110758663959 2202 82-83 0.19667704485424764 2202 84-85 0.24714039325658632 2202 86-87 0.3474661276835249 2202 88-89 0.2720764965410041 2202 90-91 0.1432438371030358 2202 92-93 0.24261087036738616 2202 94-95 0.3579674779874118 2202 96-97 0.4704489983225031 2202 98-99 0.2994401655935519 2202 100-101 0.30331102989634573 1102 1 0.10117271741989953 1102 2 0.024651325349601905 1102 3 0.2529390946538186 1102 4 0.1420801719646363 1102 5 0.054670082537455755 1102 6 -0.010658788934101437 1102 7 0.03403154239001083 1102 8 0.08417310365127406 1102 9 0.04153446375544689 1102 10-11 0.0758143742330688 1102 12-13 0.0810250739141054 1102 14-15 0.017550940713967123 1102 16-17 0.043439760594239374 1102 18-19 0.05864547365154493 1102 20-21 0.04514561049828103 1102 22-23 0.10018061221436625 1102 24-25 0.09909065228406178 1102 26-27 0.14946364400925205 1102 28-29 0.10639315891388179 1102 30-31 0.024387076774722516 1102 32-33 0.054942487620373015 1102 34-35 0.0555872988223598 1102 36-37 0.07926531616988797 1102 38-39 0.0871163432608455 1102 40-41 0.1493536921009806 1102 42-43 0.03039488918145139 1102 44-45 0.07360815807363963 1102 46-47 0.13046592388271705 1102 48-49 0.06463649657185755 1102 50-51 0.15661169911827955 1102 52-53 -0.034819996768789 1102 54-55 0.07626211710816477 1102 56-57 0.09631663574907634 1102 58-59 0.09385422746118621 1102 60-61 0.004554540545598229 1102 62-63 -0.015793702143945154 1102 64-65 -0.023029613506302837 1102 66-67 -0.08356962724983674 1102 68-69 -0.17072482417119517 1102 70-71 -0.0867384618857372 1102 72-73 -0.05715016858457744 1102 74-75 -0.20467951360637215 1102 76-77 -0.19679303544022275 1102 78-79 -0.07585009113319785 1102 80-81 -0.013746462097508072 1102 82-83 -0.05515957039921915 1102 84-85 0.034189428488133444 1102 86-87 -0.031113161794984023 1102 88-89 0.13861711850353942 1102 90-91 0.12653454406253672 1102 92-93 0.22245890736866158 1102 94-95 0.0785288747165076 1102 96-97 0.021011171473041657 1102 98-99 -0.1989736428416755 1102 100-101 -0.17457071054345263 2305 1 0.10994191134171416 2305 2 0.047599443309295 2305 3 0.23947090172425334 2305 4 0.12517271717656087 2305 5 0.02999301944034727 2305 6 0.06259122342148515 2305 7 0.04965755222125523 2305 8 0.030906743909945078 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94-95 -0.04782510480880475 1213 96-97 -0.015822061206918647 1213 98-99 -0.07994124596388374 1213 100-101 0.1600666015877188 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 11.0 8 82.0 9 242.0 10 191.0 11 100.0 12 141.0 13 316.0 14 951.0 15 2419.0 16 4835.0 17 7613.0 18 9658.0 19 11449.0 20 13716.0 21 16972.0 22 20622.0 23 26006.0 24 32170.0 25 39957.0 26 50715.0 27 66845.0 28 94116.0 29 129561.0 30 128513.0 31 134330.0 32 175839.0 33 246925.0 34 375654.0 35 637110.0 36 1181014.0 37 1596956.0 38 314121.0 39 47.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.49775029667762 24.956830628052693 24.305740462534633 14.239678612735048 2 30.800964882481324 23.295019906200125 32.77460864863625 13.1294065626823 3 36.80508046800315 18.242328021439334 28.536818071155505 16.415773439402017 4 29.828394455820877 17.657093536868114 33.82071489147256 18.693797115838443 5 31.479168754231136 16.60756313405952 35.58053969424332 16.33272841746602 6 26.851816166989117 17.499051078574453 39.457290263925174 16.191842490511256 7 33.009324527743566 20.191938745641494 29.319876665594453 17.478860061020487 8 29.659420397477287 28.64475596598509 24.534680704625153 17.16114293191247 9 30.520133020078035 25.166749793248865 25.416881533058465 18.896235653614635 10-11 24.938811183881157 27.31997215366021 29.408983433759005 18.332233228699625 12-13 23.969282572900358 26.16779172715122 28.813088370224406 21.049837329724017 14-15 23.22007438340787 26.897819351304342 29.572123386293082 20.309982878994706 16-17 23.171044426442563 26.79779485512569 30.611424995163745 19.419735723268005 18-19 20.906821085964665 27.035678505609024 31.883120704121314 20.174379704304993 20-21 22.427511145009294 27.281533284065247 31.145123972659782 19.145831598265676 22-23 21.025495013627058 29.855258227886655 30.12556218542009 18.993684573066197 24-25 21.52915186258377 26.676376152265085 31.542448982430994 20.252023002720147 26-27 21.243609230344067 26.702245358555192 30.237441943752767 21.816703467347974 28-29 21.99473886308871 28.386573713961283 29.783928669624192 19.834758753325808 30-31 20.166981971150907 27.753127022744223 30.749603746580544 21.330287259524322 32-33 19.87389261950629 29.264191568764986 30.17494933915777 20.68696647257095 34-35 21.532394833280286 28.036901058562037 30.30830593414758 20.1223981740101 36-37 19.89290961161757 28.84627369609608 31.715105313319235 19.545711378967116 38-39 20.436268741273906 29.848460536788078 29.516942992386696 20.19832772955132 40-41 20.478315981826153 27.830573589080654 32.2694887795258 19.421621649567392 42-43 20.340374564111936 29.39861194496622 31.391216241558013 18.869797249363835 44-45 19.996405465046717 28.451712348468355 31.569595423059095 19.982286763425833 46-47 21.687741366864852 27.978397668533443 30.302776498571976 20.031084466029732 48-49 20.85098342787608 30.137614571098354 30.245491535333795 18.76591046569177 50-51 21.59154104472982 29.192271407305352 29.8230758326468 19.393111715318025 52-53 19.927136892156078 28.765802618924496 29.153712750038046 22.153347738881376 54-55 21.711138395347838 28.61862412055417 30.144295718093023 19.525941766004966 56-57 20.540109377460347 28.43992582826282 29.602861581343316 21.41710321293352 58-59 20.371399827220475 28.271395286328616 30.310641189912978 21.04656369653793 60-61 21.214191838990377 28.333311242658727 30.57041035919155 19.882086559159344 62-63 20.168360052727195 28.361304824249096 30.066441044794146 21.403894078229555 64-65 21.65230817305098 28.167543982189592 29.89019658423216 20.28995126052727 66-67 20.90640851498883 30.930533138285455 28.188925162896915 19.9741331838288 68-69 21.42713020147656 30.85724429374137 28.02026330372823 19.69536220105384 70-71 20.729438048979905 31.65241112779175 28.260181769991 19.35796905323734 72-73 20.272794189060498 31.742004124264987 28.257627318794327 19.727574367880187 74-75 21.288675874355274 30.92062594832179 27.82029418495521 19.97040399236773 76-77 21.70724096201918 29.611107491302352 28.433428567211056 20.24822297946741 78-79 21.473248445188652 29.519308447514536 29.15972732830608 19.847715778990736 80-81 20.500869516173 30.1142309246952 29.03505325199049 20.349846307141313 82-83 19.562236159504163 29.907927897830977 29.223851984524206 21.305983958140654 84-85 19.398322637920625 29.31526672478594 29.891978619076294 21.394432018217138 86-87 19.662835681280818 29.402469692910095 30.468545964773735 20.46614866103535 88-89 19.781643315964264 29.973370562837715 30.037666669017014 20.207319452181004 90-91 20.20073620134884 29.670431853330626 29.399915324904246 20.72891662041629 92-93 19.580544437580915 30.422812109519338 29.204291491200113 20.792351961699634 94-95 19.433058108751396 30.75171879423445 29.326776483835392 20.488446613178755 96-97 20.147184372543634 31.112617576375285 28.495454904453325 20.24474314662775 98-99 20.411956651641823 30.428316704828212 28.26358801664421 20.896138626885758 100-101 20.218041006278593 30.341902641608705 28.33267566010131 21.10738069201139 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5019.0 1 3007.0 2 1157.5 3 1916.5 4 1985.0 5 1285.5 6 1130.5 7 1213.5 8 1283.5 9 1264.5 10 1207.0 11 1132.0 12 1050.0 13 976.5 14 976.0 15 1030.0 16 1086.0 17 1116.0 18 1231.0 19 1395.0 20 1571.0 21 1897.5 22 2339.0 23 2994.0 24 3899.0 25 4970.0 26 6409.5 27 8406.5 28 10670.0 29 13288.0 30 16812.5 31 21826.5 32 33505.5 33 54991.0 34 84600.0 35 126269.0 36 181151.0 37 271214.0 38 379686.0 39 439178.0 40 437090.0 41 384611.5 42 336127.0 43 328196.0 44 319836.0 45 307364.0 46 302744.0 47 270067.0 48 211650.5 49 168473.0 50 139460.0 51 115540.0 52 93013.5 53 66574.5 54 43865.5 55 30337.0 56 20997.0 57 14562.0 58 10203.0 59 7008.5 60 4949.5 61 3756.5 62 2720.5 63 1794.5 64 1265.0 65 889.5 66 633.0 67 484.0 68 360.0 69 257.0 70 191.0 71 135.0 72 106.0 73 84.5 74 55.5 75 40.0 76 35.0 77 24.5 78 15.0 79 12.0 80 9.5 81 8.0 82 7.0 83 4.5 84 1.5 85 1.0 86 2.0 87 1.0 88 0.0 89 0.5 90 0.5 91 1.0 92 1.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1639157188575644 2 0.0 3 3.759966024946998E-5 4 0.022315398358060436 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.1279898074840997E-4 12-13 2.8199745187102494E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 3.759966024946998E-5 28-29 8.459923556130746E-5 30-31 0.0 32-33 0.0 34-35 0.0 36-37 8.553922706754421E-4 38-39 4.0419634768180235E-4 40-41 2.725975368086574E-4 42-43 5.169953284302123E-4 44-45 2.0679813137208493E-4 46-47 0.0026601759626500017 48-49 0.003280570356766256 50-51 0.0036659668743233235 52-53 0.003412169167639401 54-55 0.0055177501416097206 56-57 0.008638521942315729 58-59 0.01613965416208499 60-61 0.0039573642412567165 62-63 0.002265379530030567 64-65 0.009587913363614847 66-67 0.02230599844299807 68-69 0.02459017780315337 70-71 0.024862775339962027 72-73 0.021779603199505487 74-75 0.02349978765591874 76-77 0.023245989949234817 78-79 0.014786066393104072 80-81 0.002763575028336044 82-83 9.493914212991172E-4 84-85 0.004699957531183748 86-87 0.013573477350058665 88-89 0.015688458239091353 90-91 0.022982792327488528 92-93 0.023161390713673513 94-95 0.024392779586843653 96-97 0.025887366081760083 98-99 0.031423916053494545 100-101 0.02268199504549277 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5319197.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.39209513512483 #Duplication Level Percentage of deduplicated Percentage of total 1 85.36171566605044 28.504065304182326 2 6.430179606706879 4.294343383261914 3 2.0791227370603234 2.082787927005584 4 1.20883882277989 1.6146264389319358 5 0.8760669610524818 1.462685565410209 6 0.577428618405669 1.1568930815727476 7 0.4427862890770761 1.0349893322573427 8 0.2994849774987481 0.8000344688143133 9 0.2835342207573223 0.8521021506232793 >10 1.9003840149366038 12.933152749508109 >50 0.24544892563518206 5.698636714119508 >100 0.23438607981480014 16.645986975518355 >500 0.042306178988759185 9.712813863433002 >1k 0.017866937486651274 9.610955926292252 >5k 2.249839170810417E-4 0.5327598181698201 >10k+ 2.2497983189113114E-4 3.063166300898911 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT 122265 2.2985612302007237 TruSeq Adapter, Index 2 (97% over 37bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15908 0.29906769762428426 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 13204 0.24823295696700085 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 11719 0.22031520923176937 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 8952 0.16829607927662765 No Hit TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA 8172 0.15363221177933437 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 5921 0.11131379416855589 No Hit GTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGT 5321 0.1000338960937149 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0018987828425982344 0.0 0.0 2 0.0020491814835961143 0.0 0.0 3 0.002199580124593994 0.0 0.0 4 0.0023311789354671392 0.0 0.0 5 0.002462777746340284 0.0 0.0 6 0.002876374009084454 0.0 0.0 7 0.0032899702718286235 0.0 0.0 8 0.0038915648358201437 0.0 0.0 9 0.004267561438314843 0.0 0.0 10-11 0.007407133069145587 0.0 0.0 12-13 0.01118589892421732 0.0 0.0 14-15 0.016036255096398947 0.0 0.0 16-17 0.02230599844299807 0.0 0.0 18-19 0.029966929218827577 0.0 1.879983012473499E-5 20-21 0.03590767553824384 0.0 1.879983012473499E-5 22-23 0.042384217016215045 0.0 1.879983012473499E-5 24-25 0.049885349235984304 0.0 1.879983012473499E-5 26-27 0.057696678652811695 0.0 2.8199745187102487E-5 28-29 0.06583700509682194 0.0 5.639949037420498E-5 30-31 0.09420594875504704 0.0 5.639949037420498E-5 32-33 0.10436725693746632 0.0 5.639949037420498E-5 34-35 0.11363557318896067 0.0 5.639949037420498E-5 36-37 0.12371228213581863 0.0 5.639949037420498E-5 38-39 0.13547157587884034 0.0 5.639949037420498E-5 40-41 0.1477196652051052 0.0 5.639949037420498E-5 42-43 0.16102054501835522 0.0 5.639949037420498E-5 44-45 0.17524261650771722 0.0 5.639949037420498E-5 46-47 0.19031068035269233 0.0 5.639949037420498E-5 48-49 0.20475834980355118 0.0 5.639949037420498E-5 50-51 0.22068180591920172 0.0 5.639949037420498E-5 52-53 0.2382126475105171 0.0 5.639949037420498E-5 54-55 0.25530169309390116 0.0 5.639949037420498E-5 56-57 0.27480651684831375 0.0 5.639949037420498E-5 58-59 0.29482833593115654 0.0 5.639949037420498E-5 60-61 0.3164951401499136 0.0 5.639949037420498E-5 62-63 0.33970353043889895 0.0 5.639949037420498E-5 64-65 0.36329731724544134 0.0 5.639949037420498E-5 66-67 0.3884138902920873 0.0 5.639949037420498E-5 68-69 0.41604024066038536 0.0 5.639949037420498E-5 70-71 0.44541497523028384 0.0 5.639949037420498E-5 72-73 0.47601169875828997 0.0 5.639949037420498E-5 74-75 0.5080466092908384 0.0 5.639949037420498E-5 76-77 0.5417171050442389 0.0 7.519932049893996E-5 78-79 0.5795705630003927 0.0 9.399915062367497E-5 80-81 0.6196706006564525 0.0 1.0339906568604247E-4 82-83 0.6626094126613472 0.0 1.1279898074840996E-4 84-85 0.7102857818576751 0.0 1.1279898074840996E-4 86-87 0.7614683193722662 0.0 1.1279898074840996E-4 88-89 0.8163638233364923 0.0 1.3159881087314494E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAAGA 18540 0.0 66.947914 4 TCGGAAG 18575 0.0 66.20802 3 GAAGAGC 18905 0.0 65.693245 6 GATCGGA 18925 0.0 65.04344 1 ATCGGAA 19365 0.0 63.531574 2 GGAAGAG 20200 0.0 61.575768 5 CGCGTTG 40 7.5562257E-6 59.365715 6 AACGCCG 155 0.0 46.047123 1 GCCGTCT 14290 0.0 43.30987 50-51 CGTCTTC 14295 0.0 43.296757 52-53 AGTCGCC 55 4.9154467E-5 43.25639 1 ATGCCGT 14415 0.0 42.768147 48-49 TGCCGTC 14440 0.0 42.596832 50-51 TCGTATG 14510 0.0 42.552402 44-45 TATGCCG 14375 0.0 42.474144 48-49 CGTATGC 14465 0.0 42.27475 46-47 CTGCTTG 14640 0.0 42.21235 58-59 TCTCGTA 14545 0.0 42.172253 42-43 CTCGTAT 14505 0.0 42.010426 44-45 ATCTCGT 14580 0.0 41.826702 42-43 >>END_MODULE