FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n01_34298-1.341000000357c1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n01_34298-1.341000000357c1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5319197
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT1222652.2985612302007237TruSeq Adapter, Index 2 (97% over 37bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159080.29906769762428426No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC132040.24823295696700085No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG117190.22031520923176937No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA89520.16829607927662765No Hit
TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA81720.15363221177933437No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA59210.11131379416855589No Hit
GTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGT53210.1000338960937149No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA185400.066.9479144
TCGGAAG185750.066.208023
GAAGAGC189050.065.6932456
GATCGGA189250.065.043441
ATCGGAA193650.063.5315742
GGAAGAG202000.061.5757685
CGCGTTG407.5562257E-659.3657156
AACGCCG1550.046.0471231
GCCGTCT142900.043.3098750-51
CGTCTTC142950.043.29675752-53
AGTCGCC554.9154467E-543.256391
ATGCCGT144150.042.76814748-49
TGCCGTC144400.042.59683250-51
TCGTATG145100.042.55240244-45
TATGCCG143750.042.47414448-49
CGTATGC144650.042.2747546-47
CTGCTTG146400.042.2123558-59
TCTCGTA145450.042.17225342-43
CTCGTAT145050.042.01042644-45
ATCTCGT145800.041.82670242-43