FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n01_34274-2.3410000003583d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n01_34274-2.3410000003583d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4624694
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT309200.6685847755548799TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTCG200.002061196671.365441
CGAGGCG1350.063.4359441
CGAGCGG1100.060.552491
CGCGTGG407.476967E-659.47121
GTTCGCG250.00499015557.0923541
CGAGCGT451.5022253E-552.8632851
CGACGGG752.3537723E-950.7487531
CGGTGCG405.347034E-447.5769581
CGACGGT652.562383E-643.917191
CGGGTCG704.2653173E-640.780251
TCGCCGG608.2922204E-539.58532
GGTACGG1101.5970727E-938.92661
CGCGCAA500.001603517438.061571
TGCGGCC1203.79805E-935.626772
CGGCGAG702.0348458E-433.9835431
CGCGATA702.0561702E-433.9232865
CGGGTGG3550.032.1647071
CGCGACC600.0039209831.7179741
AACGCGG600.0039209831.7179741
CGTCCCG600.0039209831.7179741
Produced by FastQC (version 0.11.2)