FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n01_34271-1.341000000358dd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n01_34271-1.341000000358dd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5220003
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT3499966.704900361168374TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA452550.081.387371
TCGGAAG452100.081.242263
CGGAAGA457950.080.712734
ATCGGAA455900.080.606782
GAAGAGC472400.078.146196
GGAAGAG522000.070.930065
GAGCACA535150.068.823279
AGAGCAC536050.068.73438
AAGAGCA555800.066.3516857
GCCGTCT403500.045.34462750-51
ATGCCGT402900.045.29820648-49
TCGTATG407750.045.12460344-45
TGCCGTC403550.045.1153750-51
TATGCCG404000.045.06905748-49
TCTCGTA405350.044.94631642-43
CGTATGC406750.044.94405446-47
GTATGCC407750.044.88624646-47
CGTATCT402750.044.81064638-39
TATCTCG403500.044.79273640-41
TCGTATC401600.044.6551438-39
Produced by FastQC (version 0.11.2)