##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n01_34271-1.341000000358dd.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5220003 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.656861116746484 33.0 33.0 33.0 33.0 33.0 2 32.69884327652685 33.0 33.0 33.0 33.0 33.0 3 32.541306968597524 33.0 33.0 33.0 33.0 33.0 4 36.58232878410223 37.0 37.0 37.0 37.0 37.0 5 36.65791743797848 37.0 37.0 37.0 37.0 37.0 6 36.53852612728384 37.0 37.0 37.0 37.0 37.0 7 36.41652868781876 37.0 37.0 37.0 37.0 37.0 8 36.364347491754316 37.0 37.0 37.0 37.0 37.0 9 36.42760856650849 37.0 37.0 37.0 37.0 37.0 10-11 36.35515152002786 37.0 37.0 37.0 37.0 37.0 12-13 36.33583151197423 37.0 37.0 37.0 37.0 37.0 14-15 38.16782634032969 40.0 37.0 40.0 37.0 40.0 16-17 38.19248216907155 40.0 37.0 40.0 37.0 40.0 18-19 38.145091391709926 40.0 37.0 40.0 37.0 40.0 20-21 38.13149705086376 40.0 37.0 40.0 37.0 40.0 22-23 37.933152624624924 40.0 37.0 40.0 33.0 40.0 24-25 37.94611803862948 40.0 37.0 40.0 33.0 40.0 26-27 37.894817301829136 40.0 37.0 40.0 33.0 40.0 28-29 37.67911139131529 40.0 37.0 40.0 33.0 40.0 30-31 37.67089942285474 40.0 37.0 40.0 33.0 40.0 32-33 37.52806396854561 40.0 37.0 40.0 33.0 40.0 34-35 37.33704185610621 40.0 37.0 40.0 33.0 40.0 36-37 37.00203994135636 37.0 37.0 40.0 33.0 40.0 38-39 37.17397413373134 40.0 37.0 40.0 33.0 40.0 40-41 36.96268469577508 38.5 37.0 40.0 33.0 40.0 42-43 36.73961806152219 37.0 37.0 40.0 33.0 40.0 44-45 36.755407803405475 37.0 37.0 40.0 33.0 40.0 46-47 36.74755397650155 37.0 37.0 40.0 33.0 40.0 48-49 36.44224284928572 37.0 37.0 40.0 33.0 40.0 50-51 36.34526340310532 37.0 37.0 40.0 33.0 40.0 52-53 36.25422562017685 37.0 37.0 40.0 33.0 40.0 54-55 35.9797753947651 37.0 37.0 40.0 30.0 40.0 56-57 35.83507892619985 37.0 37.0 40.0 27.0 40.0 58-59 35.55428445156066 37.0 35.0 40.0 27.0 40.0 60-61 35.2473923865561 37.0 33.0 40.0 27.0 40.0 62-63 35.02347824321174 37.0 33.0 38.5 27.0 40.0 64-65 34.729211649878366 37.0 33.0 37.0 27.0 40.0 66-67 34.12856707170475 37.0 33.0 37.0 27.0 40.0 68-69 33.933916896216346 37.0 33.0 37.0 27.0 40.0 70-71 33.493813605087965 37.0 33.0 37.0 27.0 40.0 72-73 32.69364854771156 37.0 33.0 37.0 22.0 37.0 74-75 31.92761833661781 37.0 33.0 37.0 18.5 37.0 76-77 29.409102159519833 33.0 27.0 35.0 15.0 37.0 78-79 31.478380567980516 33.0 33.0 37.0 15.0 37.0 80-81 31.744147177693193 37.0 33.0 37.0 15.0 37.0 82-83 31.638570322660733 37.0 33.0 37.0 15.0 37.0 84-85 31.477793307781624 33.0 33.0 37.0 15.0 37.0 86-87 31.32295412473901 33.0 33.0 37.0 15.0 37.0 88-89 31.20384001695018 33.0 33.0 37.0 15.0 37.0 90-91 31.07335819538801 33.0 33.0 37.0 15.0 37.0 92-93 30.92318931617472 33.0 33.0 37.0 15.0 37.0 94-95 30.802815055853415 33.0 33.0 37.0 15.0 37.0 96-97 30.661944064016822 33.0 33.0 37.0 15.0 37.0 98-99 30.48556083205316 33.0 27.0 37.0 15.0 37.0 100-101 28.8760705693081 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 0.14877760680247576 2306 2 0.13197663270693738 2306 3 0.35689526071929123 2306 4 0.24972174662872249 2306 5 0.15000400415526371 2306 6 0.18402090272756766 2306 7 0.2609048976149495 2306 8 0.17811151761077326 2306 9 0.19741680731016942 2306 10-11 0.20869125098717944 2306 12-13 0.22429281535743684 2306 14-15 0.3750129232060786 2306 16-17 0.35596416061405733 2306 18-19 0.4441479083541253 2306 20-21 0.3668540972695169 2306 22-23 0.4884316877090171 2306 24-25 0.5008876884755651 2306 26-27 0.4466456604688318 2306 28-29 0.5290531897280957 2306 30-31 0.48463802378842047 2306 32-33 0.49610870271398966 2306 34-35 0.5143116119784921 2306 36-37 0.48850230655700955 2306 38-39 0.471549530828554 2306 40-41 0.5491976599964516 2306 42-43 0.5409943324615867 2306 44-45 0.5471683660093873 2306 46-47 0.5620009327800517 2306 48-49 0.5400258199356287 2306 50-51 0.5554720598399498 2306 52-53 0.47218082667968986 2306 54-55 0.5128220580003173 2306 56-57 0.6061287867341107 2306 58-59 0.5863173005971234 2306 60-61 0.6192410530718035 2306 62-63 0.5992908698083923 2306 64-65 0.5203552673594274 2306 66-67 0.36548234923235867 2306 68-69 0.4021951176113845 2306 70-71 0.34329675170913987 2306 72-73 0.2551441956994509 2306 74-75 0.28630776755872134 2306 76-77 0.1789858110108824 2306 78-79 0.42175306525548706 2306 80-81 0.5800939496968631 2306 82-83 0.5152517299959989 2306 84-85 0.609995641185062 2306 86-87 0.5222439035580884 2306 88-89 0.7151508497058465 2306 90-91 0.6029771850282444 2306 92-93 0.5734165693846371 2306 94-95 0.5738769215822437 2306 96-97 0.6590733017648631 2306 98-99 0.4226346534392995 2306 100-101 0.4320601294505799 2307 1 0.14509432020781787 2307 2 0.14790450824337142 2307 3 0.33673037455402266 2307 4 0.24977000359213264 2307 5 0.17751465884960993 2307 6 0.20412472906158996 2307 7 0.18304844238436146 2307 8 0.19157988837197593 2307 9 0.19574147819029264 2307 10-11 0.22732010077260867 2307 12-13 0.16489926504832653 2307 14-15 0.43646582711809856 2307 16-17 0.392837712597391 2307 18-19 0.4293064915690721 2307 20-21 0.4755746352854189 2307 22-23 0.40618150982302126 2307 24-25 0.4314803873939752 2307 26-27 0.436914311415876 2307 28-29 0.4916313918607571 2307 30-31 0.4670305104746717 2307 32-33 0.4926077338815915 2307 34-35 0.4824916227624456 2307 36-37 0.4585182978313611 2307 38-39 0.4334517046077906 2307 40-41 0.46655625601426465 2307 42-43 0.5021068524352899 2307 44-45 0.4783781262264455 2307 46-47 0.5167756638582759 2307 48-49 0.5015365214096832 2307 50-51 0.43579355954818766 2307 52-53 0.408273465276892 2307 54-55 0.5310862107089847 2307 56-57 0.5343754832248919 2307 58-59 0.3987453152416336 2307 60-61 0.4787892841832715 2307 62-63 0.4801239302600919 2307 64-65 0.440895191379326 2307 66-67 0.3331552310348016 2307 68-69 0.3195420187018385 2307 70-71 0.2525593803841204 2307 72-73 -0.18264106819966486 2307 74-75 -0.08607447683325731 2307 76-77 -0.2248744998526 2307 78-79 0.21570414106570013 2307 80-81 0.23229924368271426 2307 82-83 0.3414928695497217 2307 84-85 0.3379212195915464 2307 86-87 0.3269356622043311 2307 88-89 0.3205948999423285 2307 90-91 0.25320825217189835 2307 92-93 0.32990602302225014 2307 94-95 0.22978562974825678 2307 96-97 0.3969674515928858 2307 98-99 0.08991649380231337 2307 100-101 0.050567844409499685 2201 1 0.14320784536245412 2201 2 0.11474970705150866 2201 3 0.32400679240369357 2201 4 0.21914145437643384 2201 5 0.11570559356631094 2201 6 0.16469612061155914 2201 7 0.1387029789306169 2201 8 0.16511145234940017 2201 9 0.1750799162432628 2201 10-11 0.1796729303402671 2201 12-13 0.24953220593487657 2201 14-15 0.29346082979925825 2201 16-17 0.3518424600213663 2201 18-19 0.3479952145426779 2201 20-21 0.40634479159118797 2201 22-23 0.3591842106543268 2201 24-25 0.31114707276419296 2201 26-27 0.3352977461629436 2201 28-29 0.451511407618014 2201 30-31 0.45962393326497164 2201 32-33 0.43188735987227744 2201 34-35 0.45298386936934065 2201 36-37 0.4064511119772618 2201 38-39 0.3816349342328209 2201 40-41 0.5339425176969996 2201 42-43 0.5094484685493441 2201 44-45 0.520189759873638 2201 46-47 0.6125856153428018 2201 48-49 0.5086720321062828 2201 50-51 0.5798949416634684 2201 52-53 0.4955752486122549 2201 54-55 0.5746014389665461 2201 56-57 0.5713607909108376 2201 58-59 0.6059342756732065 2201 60-61 0.5978572746756967 2201 62-63 0.5069417274021504 2201 64-65 0.5882678823825955 2201 66-67 0.3972064953466372 2201 68-69 0.4496626797431702 2201 70-71 0.4471987884572215 2201 72-73 0.35582929178197986 2201 74-75 0.24919777841583723 2201 76-77 0.16189148459723057 2201 78-79 0.47187602361280057 2201 80-81 0.44709631690480833 2201 82-83 0.5926521619076262 2201 84-85 0.5571575131185149 2201 86-87 0.6709167245698744 2201 88-89 0.6422598302638889 2201 90-91 0.4873002169529208 2201 92-93 0.48374121504505396 2201 94-95 0.5286699243766151 2201 96-97 0.541118118528729 2201 98-99 0.44574237029987174 2201 100-101 0.32683491838936973 1101 1 0.17436873720287593 1101 2 0.12066780126703236 1101 3 0.3045328080166243 1101 4 0.16446265151914474 1101 5 0.09060867434978803 1101 6 0.16896299654523972 1101 7 0.09233767908865076 1101 8 0.12665064508713186 1101 9 0.1363401523059622 1101 10-11 0.1580455246427448 1101 12-13 0.17192473065211544 1101 14-15 0.25603721207954067 1101 16-17 0.21967348950921206 1101 18-19 0.22179621392589866 1101 20-21 0.258991936511471 1101 22-23 0.2590191860424014 1101 24-25 0.25539122169519146 1101 26-27 0.2170976459221734 1101 28-29 0.25519085426302723 1101 30-31 0.2848112366045612 1101 32-33 0.31085213625866004 1101 34-35 0.2950787826902399 1101 36-37 0.27596052629246515 1101 38-39 0.2811691062904913 1101 40-41 0.28818697988040753 1101 42-43 0.26104759511520115 1101 44-45 0.2548866233528244 1101 46-47 0.3057984887207752 1101 48-49 0.40946588393357075 1101 50-51 0.30564734155502293 1101 52-53 0.30066381803169406 1101 54-55 0.31008869769467395 1101 56-57 0.33566967507333345 1101 58-59 0.5051519817797967 1101 60-61 0.37280415678801404 1101 62-63 0.3791707127559789 1101 64-65 0.22895418601491713 1101 66-67 0.08034106642307393 1101 68-69 0.10639820073054551 1101 70-71 0.12698290893626307 1101 72-73 0.05021159582909718 1101 74-75 -0.037645944275844556 1101 76-77 -0.2119705720020768 1101 78-79 0.11697724194865344 1101 80-81 0.15087052240486898 1101 82-83 0.20906710145530738 1101 84-85 0.2240026616487718 1101 86-87 0.3063371741194949 1101 88-89 0.3698959843451881 1101 90-91 0.23880890538666932 1101 92-93 0.18762611206602386 1101 94-95 0.34286447140822673 1101 96-97 0.3319961272371472 1101 98-99 0.10100914253620275 1101 100-101 0.11710670266431578 2304 1 0.14298612214607687 2304 2 0.10796605695234973 2304 3 0.3167889557740864 2304 4 0.22703423499648778 2304 5 0.13179750324110273 2304 6 0.14390583939346868 2304 7 0.14773447031505071 2304 8 0.17636969301940297 2304 9 0.12514203475458174 2304 10-11 0.13449373836963474 2304 12-13 0.11503328239852095 2304 14-15 0.2381466747524854 2304 16-17 0.25973382593412 2304 18-19 0.25869696080592064 2304 20-21 0.2757594628775095 2304 22-23 0.3131516929640483 2304 24-25 0.359182430157837 2304 26-27 0.32876951503578766 2304 28-29 0.2921228955557069 2304 30-31 0.2667024855306792 2304 32-33 0.31155685852572645 2304 34-35 0.22411252285093752 2304 36-37 0.2388808331770349 2304 38-39 0.33436223268204657 2304 40-41 0.37852238821270134 2304 42-43 0.30967816557048167 2304 44-45 0.37601822532776197 2304 46-47 0.38870471311965815 2304 48-49 0.3267144373144575 2304 50-51 0.28635578127939 2304 52-53 0.2790123700172913 2304 54-55 0.31803822598622133 2304 56-57 0.33306466437941395 2304 58-59 0.22420444875621826 2304 60-61 0.3087649251555433 2304 62-63 0.32483005812470367 2304 64-65 0.2496534467093312 2304 66-67 0.0630491593176501 2304 68-69 0.26612807214439727 2304 70-71 0.22454996768005486 2304 72-73 -0.16158241119121186 2304 74-75 -0.10255813055931995 2304 76-77 -0.21406440740144106 2304 78-79 0.13294546516352668 2304 80-81 0.13425308962307625 2304 82-83 0.1712941972341504 2304 84-85 0.01264259052873129 2304 86-87 0.11338315099352414 2304 88-89 0.23585184925697789 2304 90-91 0.16385838843087797 2304 92-93 0.283557688925697 2304 94-95 0.33092934053977885 2304 96-97 0.15782632614602932 2304 98-99 0.15139309707359772 2304 100-101 0.1127098376867508 2202 1 0.08460154679843868 2202 2 0.08478347877661463 2202 3 0.276045451419094 2202 4 0.175957576943091 2202 5 0.05878788125747292 2202 6 0.0708381815104886 2202 7 0.05329071677417119 2202 8 0.110324378957678 2202 9 0.06187599542835187 2202 10-11 0.12320276203077185 2202 12-13 0.11655672475644252 2202 14-15 0.0820374751412345 2202 16-17 0.05133451131045064 2202 18-19 0.024792941128993107 2202 20-21 0.06842298823598014 2202 22-23 0.08662030521514907 2202 24-25 0.12230821606064524 2202 26-27 0.13496277458061456 2202 28-29 0.23957461291166027 2202 30-31 0.21483658530637229 2202 32-33 0.07437795595695462 2202 34-35 0.18502856885589836 2202 36-37 0.2120399206316108 2202 38-39 0.2542455160400223 2202 40-41 0.25571704338273094 2202 42-43 0.27099263263050233 2202 44-45 0.3148686539182748 2202 46-47 0.304312915526765 2202 48-49 0.32654908106155034 2202 50-51 0.24727090607959923 2202 52-53 0.3247273861153417 2202 54-55 0.2991394802064846 2202 56-57 0.3288358739673143 2202 58-59 0.1882933542267935 2202 60-61 0.17606215647465717 2202 62-63 0.16079527343327982 2202 64-65 0.07922227682536231 2202 66-67 0.1611929317432228 2202 68-69 -0.045581822055240195 2202 70-71 0.06042467462179957 2202 72-73 0.029439296998752695 2202 74-75 -0.09895575937828838 2202 76-77 -0.12256528484896734 2202 78-79 0.10901256571763085 2202 80-81 0.17789585563246035 2202 82-83 0.1784968668855207 2202 84-85 0.2356644184753165 2202 86-87 0.2726725797083951 2202 88-89 0.33624681579421534 2202 90-91 0.2423989237429609 2202 92-93 0.27172205027747154 2202 94-95 0.2689200547958812 2202 96-97 0.32073839254646686 2202 98-99 0.10859484552021215 2202 100-101 0.15855013763101766 1102 1 0.1377024037464878 1102 2 0.06732861938574786 1102 3 0.27795261456297027 1102 4 0.17755178481798595 1102 5 0.08388316583584299 1102 6 0.0611473597331198 1102 7 0.07998119027843842 1102 8 0.0884910889540933 1102 9 0.08811322337064809 1102 10-11 0.13131801590224512 1102 12-13 0.11577090290548853 1102 14-15 0.13270949507618468 1102 16-17 0.10633860719837429 1102 18-19 0.11519458340836763 1102 20-21 0.0810312104244062 1102 22-23 0.1368778311026304 1102 24-25 0.07499408843571587 1102 26-27 0.06389985487473382 1102 28-29 0.09747354503765138 1102 30-31 0.08441321274734293 1102 32-33 0.10639627217911851 1102 34-35 0.1252531314297869 1102 36-37 0.08072992323139516 1102 38-39 0.06824634012286168 1102 40-41 0.08716242582093514 1102 42-43 0.061368031795630884 1102 44-45 0.07209973557605309 1102 46-47 0.1520945139531591 1102 48-49 0.06656271767045041 1102 50-51 0.04674751537113764 1102 52-53 -0.044508080840401476 1102 54-55 0.04301404302563583 1102 56-57 -0.010764740170607467 1102 58-59 -0.04644713325745187 1102 60-61 -0.04390223137404803 1102 62-63 0.07428080047840524 1102 64-65 -0.07497073514836927 1102 66-67 -0.05136427579321179 1102 68-69 -0.050523866467415246 1102 70-71 -0.013872770230868525 1102 72-73 -0.16172413138206565 1102 74-75 -0.21875434960800533 1102 76-77 -0.328120486136104 1102 78-79 0.008688083099400501 1102 80-81 0.015934881841236148 1102 82-83 0.10378713535012807 1102 84-85 0.022521278229174158 1102 86-87 0.11326381009350683 1102 88-89 0.16123467528808888 1102 90-91 0.14227163237571716 1102 92-93 0.15270167875346274 1102 94-95 0.04062531764396837 1102 96-97 -0.03931836125387633 1102 98-99 -0.12768123204167736 1102 100-101 -0.08934967744086819 2305 1 0.10075080871490627 2305 2 0.04541959428685516 2305 3 0.2585471412435183 2305 4 0.1452665355182461 2305 5 0.009878923171235954 2305 6 0.03658072438354765 2305 7 0.09282621164317817 2305 8 0.06812256727032207 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0.2326969414343658 1213 100-101 0.43135479698802115 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 3.0 8 29.0 9 55.0 10 50.0 11 34.0 12 81.0 13 261.0 14 736.0 15 1700.0 16 3475.0 17 5622.0 18 7866.0 19 9982.0 20 13233.0 21 17323.0 22 22308.0 23 28533.0 24 36512.0 25 47662.0 26 63660.0 27 93252.0 28 154940.0 29 209908.0 30 149721.0 31 126338.0 32 163752.0 33 229837.0 34 348031.0 35 585581.0 36 1064462.0 37 1465463.0 38 368978.0 39 615.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.34249280141105 24.177165688241903 20.31834591527329 15.16199559507376 2 29.413680413593635 28.465826552206963 28.577244112694956 13.54324892150445 3 34.10845436457687 18.88269391467666 30.71644416994476 16.292407550801705 4 28.67851310281344 18.572916860276063 28.922245379769183 23.826324657141306 5 37.11750740373137 17.229894312321274 28.914906753885006 16.73769153006234 6 33.054559547188 17.795066401302833 32.25754851098744 16.89282554052172 7 30.73555704853043 25.41406585398514 25.39268655592727 18.45769054155716 8 28.08352792134411 30.92741517581503 23.030140020992327 17.958916881848534 9 34.14045930624944 22.525101997067818 24.361307838328827 18.97313085835391 10-11 26.494448127397813 29.41713263929059 27.782591000317915 16.305828232993687 12-13 22.648717375970353 28.25949108831759 27.430956923005013 21.660834612707045 14-15 22.206912524762917 28.505203924212303 27.59020445007407 21.697679100950708 16-17 25.717916254071117 24.77170990131615 28.008805742065668 21.50156810254707 18-19 21.138072142870413 24.740857428625997 31.970326453835373 22.150743974668213 20-21 24.905024000944064 25.038462621573203 31.499838218483784 18.55667515899895 22-23 21.074547275164402 32.71847736485975 27.391085790563725 18.815889569412125 24-25 20.92808184209856 24.760368911665374 31.5336408810493 22.777908365186764 26-27 20.865928704057136 24.98934626947018 27.667869890866907 26.476855135605774 28-29 24.47421904648604 29.337363603848814 27.13310946347287 19.05530788619227 30-31 20.417478687272787 26.060952455391313 30.85334433715843 22.66822452017748 32-33 20.22892515579014 29.96492530751419 27.123375216451024 22.682774320244643 34-35 24.17120258360005 30.021840983616293 27.32100920248513 18.485947230298528 36-37 20.16750168464338 30.019573951653726 31.438984779061474 18.373939584641416 38-39 20.3045877823506 25.987960090981314 31.209040736417602 22.498411390250485 40-41 24.104036747249634 25.881169141850574 30.89328364606586 19.121510464833936 42-43 20.417944970751268 29.607682573624235 30.772584016056033 19.201788439568467 44-45 20.175132754263757 25.813321614265796 31.02010102331751 22.99144460815294 46-47 23.999032918140774 25.79609439163405 27.269074243265152 22.935798446960025 48-49 20.20314751342148 29.872420472573392 30.759178076260735 19.165253937744396 50-51 23.994960710535214 26.11490495726499 30.278803709004404 19.611330623195386 52-53 20.210176796181305 25.898196021453714 26.749599026475362 27.14202815588962 54-55 24.131728714507894 25.977454182074695 30.57867318353877 19.312143919878643 56-57 20.45347625630532 25.90019372539696 30.394176531992567 23.252153486305158 58-59 20.345477711887952 26.146336089209925 30.305231694816204 23.202954504085916 60-61 23.98184666277057 26.20212264690891 30.176388496283302 19.63964219403721 62-63 20.125560850823668 26.197927358006474 30.333479282344666 23.34303250882519 64-65 24.247200937336462 26.205359336737317 30.09568690923725 19.451752816688973 66-67 20.637243715139597 33.512252026355995 26.26909639688889 19.581407861615517 68-69 20.773502503093656 33.531289724611796 26.078085484969364 19.617122287325184 70-71 20.59287469640573 33.795610996938905 26.01269467159124 19.59881963506412 72-73 20.338578878867036 33.77346352109579 26.044782661389025 19.843174938648147 74-75 20.609460236543278 33.17126384120026 25.9470900018376 20.27218592041886 76-77 20.64034566068814 32.37290394393534 26.182294853786615 20.80445554158991 78-79 20.59839713274587 31.836229222068972 26.607257286759612 20.958116358425542 80-81 20.469018881352994 31.46558821571382 26.816684251841416 21.248708651091775 82-83 20.154522626903816 31.364395906280667 26.910404750239348 21.570676716576173 84-85 20.273093637454867 31.176148336933945 26.90003077756309 21.650727248048096 86-87 20.188055592997046 31.012053541363045 27.386411220223117 21.413479645416793 88-89 20.23142579731346 31.054243804208237 27.45097757448865 21.26335282398965 90-91 20.546171075148678 30.661712054458068 27.14157223797391 21.650544632419344 92-93 20.28443419265324 30.923751493873038 26.967807071985856 21.82400724148787 94-95 20.23584408088957 30.860123541208047 26.892751695355084 22.01128068254731 96-97 20.489132850859914 31.080713377983333 26.704401739602318 21.725752031554435 98-99 20.655437377445637 30.771618793114104 26.76988261130559 21.803061218134665 100-101 20.68756591533405 30.85084816172864 26.33614597640538 22.125439946531934 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2352.0 1 1443.0 2 578.5 3 821.5 4 885.0 5 652.0 6 557.5 7 568.0 8 575.0 9 561.0 10 529.0 11 523.0 12 510.5 13 497.5 14 528.0 15 542.0 16 564.5 17 623.0 18 699.0 19 772.0 20 884.5 21 1140.0 22 1570.5 23 2317.0 24 3375.5 25 5006.5 26 8238.5 27 13093.5 28 18138.0 29 23314.0 30 28902.5 31 33892.5 32 44362.0 33 65469.0 34 96940.0 35 132162.0 36 177884.0 37 237934.0 38 285381.0 39 302482.0 40 311009.5 41 314520.5 42 312318.5 43 312752.0 44 299198.0 45 276633.5 46 255685.5 47 226418.5 48 192461.5 49 168024.0 50 149314.0 51 135343.0 52 124509.5 53 109265.5 54 91445.0 55 75675.0 56 62767.5 57 53517.5 58 46670.5 59 39659.0 60 32799.5 61 26959.5 62 21888.5 63 17719.5 64 14518.5 65 11534.0 66 8877.0 67 7215.5 68 6353.0 69 5225.0 70 3699.0 71 2604.0 72 1869.0 73 1386.0 74 1052.5 75 847.5 76 679.0 77 484.0 78 334.5 79 222.5 80 138.5 81 86.0 82 67.0 83 49.0 84 34.5 85 20.0 86 11.0 87 13.5 88 12.5 89 9.0 90 5.5 91 2.5 92 2.0 93 1.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16408036547105434 2 0.0 3 1.915707711279093E-5 4 0.022260523605063063 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.4367807834593198E-4 12-13 1.915707711279093E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 9.578538556395466E-6 28-29 1.1494246267674559E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0010632177797598966 38-39 5.747123133837279E-4 40-41 3.927200808122141E-4 42-43 6.417620832784962E-4 44-45 3.8314154225581857E-4 46-47 0.003371645571851204 48-49 0.0038314154225581863 50-51 0.00420497842625761 52-53 0.003965514962347723 54-55 0.006149421753205889 56-57 0.009607274172064651 58-59 0.016340986777210662 60-61 0.0047222195083029645 62-63 0.0027490405656854988 64-65 0.01020114356256117 66-67 0.022068952833935153 68-69 0.024157074239229363 70-71 0.02448274455014681 72-73 0.02165707567601015 74-75 0.024022974699439828 76-77 0.023821825389755524 78-79 0.015201140688999606 80-81 0.003266281647730854 82-83 0.0010632177797598966 84-85 0.005584287978378556 86-87 0.014339072218924012 88-89 0.016005737927736824 90-91 0.022260523605063063 92-93 0.022720293455770046 94-95 0.024109181546447388 96-97 0.025651326254027055 98-99 0.03171454116022539 100-101 0.022739450532882836 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5220003.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.324582817169905 #Duplication Level Percentage of deduplicated Percentage of total 1 90.99396183928707 48.522150539614614 2 4.5814110137380615 4.886036620431392 3 1.4188056914673435 2.269716647883671 4 0.6277012574748067 1.3388763075462806 5 0.4243259548646967 1.131350226082861 6 0.32642532005615094 1.0443896407773248 7 0.24761202165080737 0.9242665428531743 8 0.1264956114440709 0.5396260566766324 9 0.12402218860481995 0.5952088320681949 >10 0.8806892372328479 9.343042748362073 >50 0.1315477769986925 4.839756821051133 >100 0.10270053651431177 11.83290837620768 >500 0.012044289524076561 4.2195045754819205 >1k 0.002221394844635622 1.8192988408458164 >5k 0.0 0.0 >10k+ 3.586629748117885E-5 6.693867224117294 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT 349996 6.704900361168374 TruSeq Adapter, Index 7 (97% over 35bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0029693469524825944 0.0 0.0 2 0.003314174340512831 0.0 0.0 3 0.003812258345445395 0.0 0.0 4 0.004272028196152378 0.0 0.0 5 0.00473179804685936 0.0 0.0 6 0.005536395285596579 0.0 0.0 7 0.00637930667855938 0.0 0.0 8 0.0075095742282140455 0.0 0.0 9 0.008467428083853592 0.0 0.0 10-11 0.012289264967855382 0.0 0.0 12-13 0.016044052081962405 0.0 0.0 14-15 0.020929106745724093 0.0 1.915707711279093E-5 16-17 0.026772015265125328 0.0 1.915707711279093E-5 18-19 0.03368772010284285 0.0 1.915707711279093E-5 20-21 0.03918580123421385 0.0 1.915707711279093E-5 22-23 0.04488503167526915 0.0 1.915707711279093E-5 24-25 0.051398437893618074 0.0 4.789269278197733E-5 26-27 0.057289239105801276 0.0 5.7471231338372794E-5 28-29 0.06397505901816532 0.0 5.7471231338372794E-5 30-31 0.09099611628575693 0.0 5.7471231338372794E-5 32-33 0.10024898453123494 0.0 5.7471231338372794E-5 34-35 0.10848652768973505 0.0 5.7471231338372794E-5 36-37 0.11742330416285202 0.0 5.7471231338372794E-5 38-39 0.12772981164953354 0.0 5.7471231338372794E-5 40-41 0.13787348398075633 0.0 5.7471231338372794E-5 42-43 0.14926236632431056 0.0 7.662830845116373E-5 44-45 0.16169530937051185 0.0 7.662830845116373E-5 46-47 0.1755267190459469 0.0 8.620684700755919E-5 48-49 0.18944433556838952 0.0 9.578538556395466E-5 50-51 0.20456884794893795 0.0 9.578538556395466E-5 52-53 0.22193473835168293 0.0 9.578538556395466E-5 54-55 0.23964545614245814 0.0 9.578538556395466E-5 56-57 0.25862054102267756 0.0 9.578538556395466E-5 58-59 0.2794251267671686 0.0 9.578538556395466E-5 60-61 0.30197300652892345 0.0 9.578538556395466E-5 62-63 0.3263503871549499 0.0 9.578538556395466E-5 64-65 0.35263389695369907 0.0 9.578538556395466E-5 66-67 0.37954001175861396 0.0 9.578538556395466E-5 68-69 0.4093867379003422 0.0 9.578538556395466E-5 70-71 0.44220281099455305 0.0 9.578538556395466E-5 72-73 0.477902024194239 0.0 9.578538556395466E-5 74-75 0.5165131131150691 0.0 1.0536392412035012E-4 76-77 0.5583713266065173 0.0 1.1494246267674559E-4 78-79 0.6048368171435917 0.0 1.1494246267674559E-4 80-81 0.6574708865109848 0.0 1.1494246267674559E-4 82-83 0.7157371365495384 0.0 1.2452100123314105E-4 84-85 0.778725606096395 0.0 1.3409953978953652E-4 86-87 0.8483998955556156 0.0 1.3409953978953652E-4 88-89 0.9243960204620572 0.0 1.3409953978953652E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCGGA 45255 0.0 81.38737 1 TCGGAAG 45210 0.0 81.24226 3 CGGAAGA 45795 0.0 80.71273 4 ATCGGAA 45590 0.0 80.60678 2 GAAGAGC 47240 0.0 78.14619 6 GGAAGAG 52200 0.0 70.93006 5 GAGCACA 53515 0.0 68.82327 9 AGAGCAC 53605 0.0 68.7343 8 AAGAGCA 55580 0.0 66.351685 7 GCCGTCT 40350 0.0 45.344627 50-51 ATGCCGT 40290 0.0 45.298206 48-49 TCGTATG 40775 0.0 45.124603 44-45 TGCCGTC 40355 0.0 45.11537 50-51 TATGCCG 40400 0.0 45.069057 48-49 TCTCGTA 40535 0.0 44.946316 42-43 CGTATGC 40675 0.0 44.944054 46-47 GTATGCC 40775 0.0 44.886246 46-47 CGTATCT 40275 0.0 44.810646 38-39 TATCTCG 40350 0.0 44.792736 40-41 TCGTATC 40160 0.0 44.65514 38-39 >>END_MODULE