##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l07n01_34211-2.34100000035863.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3534150 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.6671626840966 33.0 33.0 33.0 33.0 33.0 2 32.715264773707965 33.0 33.0 33.0 33.0 33.0 3 32.554414781489186 33.0 33.0 33.0 33.0 33.0 4 36.590577932459006 37.0 37.0 37.0 37.0 37.0 5 36.65556555324477 37.0 37.0 37.0 37.0 37.0 6 36.536662563841375 37.0 37.0 37.0 37.0 37.0 7 36.452558323783656 37.0 37.0 37.0 37.0 37.0 8 36.420426127923264 37.0 37.0 37.0 37.0 37.0 9 36.41460039896439 37.0 37.0 37.0 37.0 37.0 10-11 36.37125758668987 37.0 37.0 37.0 37.0 37.0 12-13 36.34876193709944 37.0 37.0 37.0 37.0 37.0 14-15 38.1746824554702 40.0 37.0 40.0 37.0 40.0 16-17 38.16417766648274 40.0 37.0 40.0 37.0 40.0 18-19 38.161000947894124 40.0 37.0 40.0 37.0 40.0 20-21 38.10213431801141 40.0 37.0 40.0 37.0 40.0 22-23 37.97472532292065 40.0 37.0 40.0 33.0 40.0 24-25 37.937202580535626 40.0 37.0 40.0 33.0 40.0 26-27 37.88361586237143 40.0 37.0 40.0 33.0 40.0 28-29 37.69342232219911 40.0 37.0 40.0 33.0 40.0 30-31 37.643691693900934 40.0 37.0 40.0 33.0 40.0 32-33 37.49974534187852 40.0 37.0 40.0 33.0 40.0 34-35 37.39478035737023 40.0 37.0 40.0 33.0 40.0 36-37 37.351562752005435 40.0 37.0 40.0 33.0 40.0 38-39 37.2049372550684 40.0 37.0 40.0 33.0 40.0 40-41 37.0985927309254 40.0 37.0 40.0 33.0 40.0 42-43 36.93668265353763 40.0 37.0 40.0 33.0 40.0 44-45 36.79744592051837 37.0 37.0 40.0 33.0 40.0 46-47 36.77008474456376 37.0 37.0 40.0 33.0 40.0 48-49 36.58243269244373 37.0 37.0 40.0 33.0 40.0 50-51 36.44616583902777 37.0 37.0 40.0 33.0 40.0 52-53 36.32709053096218 37.0 37.0 40.0 33.0 40.0 54-55 36.12210375903683 37.0 37.0 40.0 33.0 40.0 56-57 35.957360893001145 37.0 37.0 40.0 27.0 40.0 58-59 35.76974859584341 37.0 37.0 40.0 27.0 40.0 60-61 35.511006606963484 37.0 33.0 40.0 27.0 40.0 62-63 35.30344085565129 37.0 33.0 40.0 27.0 40.0 64-65 35.04416366028607 37.0 33.0 40.0 27.0 40.0 66-67 34.66304472079567 37.0 33.0 40.0 27.0 40.0 68-69 34.42726016156643 37.0 33.0 37.0 27.0 40.0 70-71 34.09839282430004 37.0 33.0 37.0 27.0 40.0 72-73 33.65041806374941 37.0 33.0 37.0 27.0 40.0 74-75 33.21185023272923 37.0 33.0 37.0 22.0 40.0 76-77 30.640756900527705 33.0 30.0 35.0 22.0 37.0 78-79 32.77842225146075 35.0 33.0 37.0 22.0 37.0 80-81 33.03001230847587 37.0 33.0 37.0 22.0 37.0 82-83 32.88252267164665 37.0 33.0 37.0 22.0 37.0 84-85 32.676908450405335 37.0 33.0 37.0 22.0 37.0 86-87 32.48180920447632 37.0 33.0 37.0 22.0 37.0 88-89 32.312731066876054 37.0 33.0 37.0 22.0 37.0 90-91 32.14270107380841 33.0 33.0 37.0 22.0 37.0 92-93 31.952456177581595 33.0 33.0 37.0 22.0 37.0 94-95 31.80764257317884 33.0 33.0 37.0 22.0 37.0 96-97 31.65841715829832 33.0 33.0 37.0 18.5 37.0 98-99 31.468934114284906 33.0 33.0 37.0 15.0 37.0 100-101 29.74285372720456 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2306 1 0.16199897513017447 2306 2 0.10249294688545518 2306 3 0.30655790516036774 2306 4 0.19953631430092855 2306 5 0.12989190376545423 2306 6 0.1392508658592959 2306 7 0.18957254911289567 2306 8 0.14029224424569264 2306 9 0.19418182437594567 2306 10-11 0.19454099798085167 2306 12-13 0.14589043909700905 2306 14-15 0.4220550796369338 2306 16-17 0.38761822549258795 2306 18-19 0.40282345289318044 2306 20-21 0.42361626331436497 2306 22-23 0.4821581637810368 2306 24-25 0.396799463285312 2306 26-27 0.48563392545199235 2306 28-29 0.49007993157871965 2306 30-31 0.4079827288713176 2306 32-33 0.5208075775991716 2306 34-35 0.47028920779311534 2306 36-37 0.5263115120784576 2306 38-39 0.4994680133703113 2306 40-41 0.43235899982519754 2306 42-43 0.5201493534731796 2306 44-45 0.4418637598852939 2306 46-47 0.5327546285708706 2306 48-49 0.44044911582573576 2306 50-51 0.45861883046660523 2306 52-53 0.4305917374214445 2306 54-55 0.45790927242128987 2306 56-57 0.5046496947233337 2306 58-59 0.31043735682780493 2306 60-61 0.443964735819911 2306 62-63 0.4384243596749684 2306 64-65 0.31251759559028613 2306 66-67 0.31113052467075875 2306 68-69 0.3447359528480476 2306 70-71 0.3841332435423368 2306 72-73 0.2683408207701987 2306 74-75 0.06721573613091181 2306 76-77 0.04636503250068458 2306 78-79 0.15010841645273842 2306 80-81 0.2889189711120821 2306 82-83 0.13572102533868957 2306 84-85 0.18956790272037694 2306 86-87 0.28472348605857434 2306 88-89 0.34772511914611215 2306 90-91 0.33426843785083094 2306 92-93 0.43400336837443376 2306 94-95 0.3490848748162776 2306 96-97 0.332839024364155 2306 98-99 0.2897308493753634 2306 100-101 0.2858791945389001 2307 1 0.14607629817966483 2307 2 0.14051648482953993 2307 3 0.3269434338948969 2307 4 0.20638592936978029 2307 5 0.10591511032310308 2307 6 0.1598115948008072 2307 7 0.22666626967943415 2307 8 0.2118360636509493 2307 9 0.123801762159502 2307 10-11 0.18205365415859376 2307 12-13 0.18649784055877916 2307 14-15 0.31853323291809943 2307 16-17 0.3996360768997249 2307 18-19 0.2742638498506693 2307 20-21 0.39721552747349875 2307 22-23 0.3078409183085711 2307 24-25 0.3126323161762201 2307 26-27 0.33443699566585394 2307 28-29 0.4489652644013873 2307 30-31 0.3456711285124925 2307 32-33 0.3224708421222857 2307 34-35 0.4222586312825811 2307 36-37 0.41972697220649025 2307 38-39 0.27995573543487495 2307 40-41 0.30036168690744347 2307 42-43 0.2952161400637081 2307 44-45 0.33008568829631457 2307 46-47 0.3865260337240528 2307 48-49 0.2284178188213204 2307 50-51 0.2810606358164591 2307 52-53 0.3074067232865829 2307 54-55 0.39555429642982176 2307 56-57 0.2813319901991562 2307 58-59 0.2691169312678596 2307 60-61 0.31885939499778004 2307 62-63 0.33228843079810133 2307 64-65 0.20184445670201967 2307 66-67 0.17043559101255568 2307 68-69 0.3390228472614041 2307 70-71 0.24089196501543597 2307 72-73 0.2782154405755648 2307 74-75 0.09940512415930414 2307 76-77 -0.023733894499908814 2307 78-79 0.3557612035960318 2307 80-81 0.23892927600797975 2307 82-83 0.36647132701578045 2307 84-85 0.45705643152077613 2307 86-87 0.3461974528696814 2307 88-89 0.4947591765723942 2307 90-91 0.4584718880725873 2307 92-93 0.4937143831027839 2307 94-95 0.47321277843715137 2307 96-97 0.5248734756513684 2307 98-99 0.35141889318356334 2307 100-101 0.3967928092926556 2201 1 0.15977095325664692 2201 2 0.07869422079316735 2201 3 0.2840865319986392 2201 4 0.20976844014295892 2201 5 0.12516034407212118 2201 6 0.14879883440464425 2201 7 0.1412182814440328 2201 8 0.1981154829673315 2201 9 0.13352199050474667 2201 10-11 0.15699280707219287 2201 12-13 0.17786711240531616 2201 14-15 0.27984301330634764 2201 16-17 0.3619308741614802 2201 18-19 0.3909218954722391 2201 20-21 0.3405052932934254 2201 22-23 0.23483847068141728 2201 24-25 0.20557408538959265 2201 26-27 0.33640546762934065 2201 28-29 0.4645615471066975 2201 30-31 0.5517776708861746 2201 32-33 0.47867777594139227 2201 34-35 0.4588170163734162 2201 36-37 0.3526364999606315 2201 38-39 0.42052562399850046 2201 40-41 0.4514612762208472 2201 42-43 0.3477222091545116 2201 44-45 0.37292259936337047 2201 46-47 0.45764825015726984 2201 48-49 0.514572170512487 2201 50-51 0.5013159999181767 2201 52-53 0.34457639045177757 2201 54-55 0.3484821407812291 2201 56-57 0.5160972603814642 2201 58-59 0.5286103886442888 2201 60-61 0.4332947102968987 2201 62-63 0.438458006755134 2201 64-65 0.45588463452045147 2201 66-67 0.5622620906058771 2201 68-69 0.5212109872022026 2201 70-71 0.3103288608394763 2201 72-73 0.20079455115858735 2201 74-75 0.17514459742594113 2201 76-77 -0.05825836889727398 2201 78-79 0.3425229016670386 2201 80-81 0.34581008818539516 2201 82-83 0.5000855002638076 2201 84-85 0.34605657145657887 2201 86-87 0.44262118723154487 2201 88-89 0.45323041539158027 2201 90-91 0.39618686757574295 2201 92-93 0.2820324296989156 2201 94-95 0.41756034024317046 2201 96-97 0.4184718186615157 2201 98-99 0.3252894738047054 2201 100-101 0.27349999487127263 1101 1 0.1679740210092575 1101 2 0.11923086710198305 1101 3 0.31844443260067123 1101 4 0.17173205139071257 1101 5 0.14562919859111645 1101 6 0.11607449538184511 1101 7 0.11823698077613187 1101 8 0.1276880551520989 1101 9 0.193966525879965 1101 10-11 0.1345895939270889 1101 12-13 0.16588449275759842 1101 14-15 0.2805373277633265 1101 16-17 0.23333019711419212 1101 18-19 0.2732527170849366 1101 20-21 0.2076134336769897 1101 22-23 0.30910225793824253 1101 24-25 0.2198056454278401 1101 26-27 0.18416784521742358 1101 28-29 0.16809282898385192 1101 30-31 0.26361380977228777 1101 32-33 0.19726109875480802 1101 34-35 0.3310648975172299 1101 36-37 0.3769784248005479 1101 38-39 0.3170496986449294 1101 40-41 0.3579057042203573 1101 42-43 0.2872783992907486 1101 44-45 0.3273608431032855 1101 46-47 0.3700063945311456 1101 48-49 0.27029768921689623 1101 50-51 0.30853527414554804 1101 52-53 0.23666418023918823 1101 54-55 0.3519701096248724 1101 56-57 0.4978575397904379 1101 58-59 0.36068597532912605 1101 60-61 0.3242413431257063 1101 62-63 0.29425433537699774 1101 64-65 0.15270972386718284 1101 66-67 0.07163555342848582 1101 68-69 0.09836680819821453 1101 70-71 0.14038048823420723 1101 72-73 0.22139037018120433 1101 74-75 0.2471212098388662 1101 76-77 -0.04289464824239886 1101 78-79 0.3291732007954167 1101 80-81 0.2171466543977445 1101 82-83 0.33124179139123555 1101 84-85 0.3701243980709563 1101 86-87 0.5557914691217576 1101 88-89 0.678467693911081 1101 90-91 0.5787121988079917 1101 92-93 0.3660531125793085 1101 94-95 0.49719588682172855 1101 96-97 0.3926212653139274 1101 98-99 0.16945280674626062 1101 100-101 0.3567421314154302 2304 1 0.11185887504726111 2304 2 0.06422780625661062 2304 3 0.30250331189494517 2304 4 0.14728693704202556 2304 5 0.046795625282214814 2304 6 0.15820547840903032 2304 7 0.13942622828415097 2304 8 0.13437986967147708 2304 9 0.14780527552132128 2304 10-11 0.11629268002608484 2304 12-13 0.08511241930259672 2304 14-15 0.1995885450278152 2304 16-17 0.27703358202574435 2304 18-19 0.25526625091355726 2304 20-21 0.1665817106259837 2304 22-23 0.28383256638948495 2304 24-25 0.23292946620905752 2304 26-27 0.20463928151415445 2304 28-29 0.17781137664908897 2304 30-31 0.15207746372090725 2304 32-33 0.2941196004346409 2304 34-35 0.248309224375852 2304 36-37 0.1974240124894706 2304 38-39 0.1889248267310606 2304 40-41 0.17012323711288246 2304 42-43 0.33388489357166407 2304 44-45 0.20796704914908304 2304 46-47 0.24695201461346983 2304 48-49 0.14525184264523716 2304 50-51 0.061142942521790644 2304 52-53 0.16114724113744927 2304 54-55 0.28910689040884563 2304 56-57 0.2339948190360488 2304 58-59 0.06178503697838522 2304 60-61 0.16677585531422068 2304 62-63 0.10551137102427788 2304 64-65 0.13061676672033684 2304 66-67 0.050172680887904164 2304 68-69 0.1565612796371525 2304 70-71 0.17596820360071064 2304 72-73 0.1273596136433568 2304 74-75 0.07059848884648545 2304 76-77 -0.06162378259830703 2304 78-79 0.21110171848683024 2304 80-81 0.2800355238946679 2304 82-83 0.30152323050421614 2304 84-85 0.345436465470371 2304 86-87 0.38082601105930536 2304 88-89 0.40263475392612236 2304 90-91 0.2791266359496021 2304 92-93 0.45711610517378176 2304 94-95 0.4891169738647676 2304 96-97 0.4219520970837891 2304 98-99 0.4578940497535875 2304 100-101 0.15590182028016386 2202 1 0.10398775619803047 2202 2 0.059390617681387425 2202 3 0.25290593594667854 2202 4 0.1573629018888525 2202 5 0.0897033274751422 2202 6 0.09837150253538596 2202 7 0.15054891202995435 2202 8 0.2065905192590094 2202 9 0.09710159024171361 2202 10-11 0.1637849591787628 2202 12-13 0.12744753516712848 2202 14-15 0.1505054604197653 2202 16-17 0.045239754558181744 2202 18-19 0.01919486675735982 2202 20-21 0.1025789953749765 2202 22-23 0.18787642494771006 2202 24-25 0.162234486314361 2202 26-27 0.12877547933366174 2202 28-29 0.19783894370127797 2202 30-31 0.10501915836916709 2202 32-33 0.18038219215208784 2202 34-35 0.09982800313471074 2202 36-37 0.075011014520733 2202 38-39 0.24020757555997108 2202 40-41 0.19726149727952702 2202 42-43 0.27520774935245385 2202 44-45 0.2913871772426049 2202 46-47 0.337054043797238 2202 48-49 0.3702186792741813 2202 50-51 0.21284075166658312 2202 52-53 0.2460713799261569 2202 54-55 0.3475871271124049 2202 56-57 0.2867802831159807 2202 58-59 0.2965441361783192 2202 60-61 0.38098791434502033 2202 62-63 0.4076504084574353 2202 64-65 0.3289777707413961 2202 66-67 0.2915126365318983 2202 68-69 0.2974680387467217 2202 70-71 0.310288924489484 2202 72-73 0.36009263814279535 2202 74-75 0.22088037750047107 2202 76-77 0.08260838172619245 2202 78-79 0.2732506531135428 2202 80-81 0.24016952464084085 2202 82-83 0.3550121207749086 2202 84-85 0.345378170482185 2202 86-87 0.47115344753072463 2202 88-89 0.6192913161281695 2202 90-91 0.42615955059537214 2202 92-93 0.5340421715834367 2202 94-95 0.4636044961464094 2202 96-97 0.4817225961925189 2202 98-99 0.3374073772833306 2202 100-101 0.3847213079060836 1102 1 0.11672779634987762 1102 2 0.11112539822893552 1102 3 0.20661975708780744 1102 4 0.17370713571767737 1102 5 0.05939707987689502 1102 6 0.05480378849696166 1102 7 0.12162265961922714 1102 8 0.15027041963359267 1102 9 0.020414889552519355 1102 10-11 0.06419758851403401 1102 12-13 0.07058670548205015 1102 14-15 0.03464972423677182 1102 16-17 0.0867969583665058 1102 18-19 0.05576518815168896 1102 20-21 0.04055261115990305 1102 22-23 0.13070942847087252 1102 24-25 0.10559587446202556 1102 26-27 0.0347923750717527 1102 28-29 0.054474323294222415 1102 30-31 0.06219149705249549 1102 32-33 0.15255041868319097 1102 34-35 0.10673897773273922 1102 36-37 -0.019029888823943963 1102 38-39 0.09204406004692345 1102 40-41 0.05850093856553684 1102 42-43 -0.03283835433843052 1102 44-45 0.031809476420541216 1102 46-47 0.12592364637298203 1102 48-49 0.12675278140639534 1102 50-51 0.11893946397036359 1102 52-53 -0.024183663296334146 1102 54-55 0.16838000482884752 1102 56-57 -0.00703512524408012 1102 58-59 0.048948241327302355 1102 60-61 0.049467863562632886 1102 62-63 0.013671209842279097 1102 64-65 -0.029864299269341643 1102 66-67 -0.11391268839995661 1102 68-69 -0.20284923893668605 1102 70-71 -0.12152016659916853 1102 72-73 -0.219032959376257 1102 74-75 -0.20413030691249645 1102 76-77 -0.2092998813653537 1102 78-79 -0.07478276395924865 1102 80-81 -0.07730591018783173 1102 82-83 -0.02070683247584526 1102 84-85 -0.07686477960096028 1102 86-87 0.06788251326410943 1102 88-89 0.06987220252143089 1102 90-91 -0.03730828662804697 1102 92-93 0.03322355700163371 1102 94-95 0.013619894270981092 1102 96-97 -0.009825382208806133 1102 98-99 -0.20640051158440897 1102 100-101 -0.32557509925803885 2305 1 0.10721371528428847 2305 2 0.05029317246454923 2305 3 0.25662009953107656 2305 4 0.1265502809905712 2305 5 0.06224137334190516 2305 6 0.07194831123781142 2305 7 0.11294273005779587 2305 8 0.0720175007076378 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-0.01952765080212515 1213 100-101 0.2574144603683237 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 7.0 8 19.0 9 103.0 10 155.0 11 138.0 12 170.0 13 265.0 14 663.0 15 1286.0 16 2434.0 17 3864.0 18 5557.0 19 7324.0 20 9661.0 21 12207.0 22 15675.0 23 19361.0 24 23842.0 25 29965.0 26 36704.0 27 45412.0 28 59561.0 29 81073.0 30 89705.0 31 92339.0 32 117093.0 33 161037.0 34 235819.0 35 372025.0 36 628712.0 37 1005392.0 38 474812.0 39 1770.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.08913667971876 29.124249239887266 15.995753344836109 17.79086073555786 2 30.95649590424855 27.17796358388863 27.42914137770044 14.436399134162386 3 35.11442368255302 22.767508756004986 24.09011964983903 18.027947911602972 4 29.92596345152955 23.604612001505636 26.58274348430261 19.886681062662205 5 34.4205820352843 21.33653070752515 26.31133370117284 17.93155355601771 6 31.711302576291327 22.054808086810123 28.138788676202196 18.095100660696346 7 32.333969978637015 24.40097335993096 22.999080401228017 20.26597626020401 8 29.140245886563953 28.60518087800461 22.76564378987875 19.48892944555268 9 30.842295884441807 23.183650948601503 25.0811934977293 20.892859669227395 10-11 25.745318069106098 29.97206821281127 27.852843070552698 16.429770647529935 12-13 24.509988118780505 29.443000280972875 27.36597975920087 18.68103184104575 14-15 23.93623643591811 29.555197147829038 27.513079524072264 18.99548689218058 16-17 24.977363722535827 28.045725280477622 27.57902182986008 19.39788916712647 18-19 22.973275044918864 28.086215921791663 29.220052912298573 19.720456120990903 20-21 24.09576560134686 28.224792382892634 29.140783498153727 18.53865851760678 22-23 22.67799895307217 31.39180566755797 27.405953340973078 18.524242038396785 24-25 22.266146032284993 28.519403534088816 29.10008064173847 20.114369791887725 26-27 22.22690667541182 28.37306802854096 27.728471550011047 21.671553746036174 28-29 23.43118769977419 29.910177747300516 27.647283438872723 19.011351114052573 30-31 21.89791038863659 28.43970968974152 29.185207192677165 20.477172728944726 32-33 21.876420072719043 29.84441803545407 27.720441973317488 20.558719918509404 34-35 21.67819702049998 29.844573659861634 29.27041862965635 19.20681068998203 36-37 21.70674298193478 28.339343566268354 29.21519312562719 20.738720326169673 38-39 22.938364312909304 29.881530939938678 27.777306971914868 19.402797775237154 40-41 21.455205509685523 31.15656142654224 27.839997849547554 19.548235214224675 42-43 21.48352127198418 29.724144429240333 29.120134731809678 19.672199566965805 44-45 21.343883191917463 28.32970067108283 29.239540380273667 21.086875756726037 46-47 22.768778856007206 28.280425672131948 27.71389187060851 21.236903601252337 48-49 21.271041217696805 29.749186194969123 29.095312528296418 19.88446005903765 50-51 22.59582625919638 28.291185625353705 29.118972835314093 19.994015280135823 52-53 21.286125938859513 28.340339305918476 27.68548802301975 22.688046732202256 54-55 22.38687481589241 28.31319386101458 29.318460222431685 19.98147110066133 56-57 21.11443439598429 28.270812926353482 29.208339588368904 21.406413089293327 58-59 20.987412372093893 28.493902306151693 29.149404748596947 21.369280573157468 60-61 22.305757141832398 28.387999438028093 29.115337093300774 20.19090632683873 62-63 20.927703343189823 28.4214852647812 29.083784892686857 21.567026499342116 64-65 22.226119521668828 28.40333285603599 29.13642761616012 20.23412000613506 66-67 20.934989578538225 30.946625898744063 27.679993977358773 20.438390545358935 68-69 20.876261852587458 31.093731758106387 27.619633212787953 20.410373176518203 70-71 20.817874799139886 31.013424637567333 27.669003755049363 20.49969680824342 72-73 20.754425152882426 31.007279913340092 27.618104901789447 20.620190031988034 74-75 20.60650238001537 30.907524759957155 27.829785704511295 20.65618715551618 76-77 20.728185915978482 30.617542061207548 27.80066417162442 20.853607851189558 78-79 20.628458407419746 30.643346910614362 27.824945614938194 20.903249067027694 80-81 20.67008778506235 30.404570759808315 27.826012770974323 21.099328684155015 82-83 20.7476169032114 30.432263063722274 27.679037675544578 21.141082357521746 84-85 20.65174761457402 30.287482031488754 27.830167857021586 21.23060249691564 86-87 20.82650786151441 29.971812340170057 27.786982171492642 21.41469762682289 88-89 20.760806828345714 30.16879980540896 27.7055505849688 21.36484278127653 90-91 20.752048319379757 29.957234314944554 27.757534796912882 21.53318256876281 92-93 20.764810416983295 29.91044933259408 27.697862087840086 21.626878162582532 94-95 20.617601566822756 29.871889031816178 27.823839989358284 21.68666941200278 96-97 20.682580912669295 29.762121245355672 27.77319192263687 21.782105919338164 98-99 20.65611471287109 29.86650136781965 27.71584731934237 21.76153659996688 100-101 20.733641343886013 29.713324902170633 27.666270297302496 21.886763456640857 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 8.0 1 6.5 2 12.0 3 26.0 4 25.0 5 15.0 6 13.0 7 18.5 8 22.0 9 22.0 10 25.5 11 30.5 12 37.0 13 51.0 14 80.0 15 120.0 16 192.0 17 318.5 18 541.5 19 910.0 20 1462.5 21 2277.5 22 3612.0 23 5485.5 24 8086.5 25 11469.5 26 15666.5 27 20626.0 28 26556.0 29 33633.0 30 41671.0 31 50585.5 32 61009.5 33 72984.0 34 85984.5 35 100466.0 36 116202.0 37 132288.0 38 147137.0 39 159244.5 40 168619.5 41 174220.5 42 175483.5 43 174116.0 44 171189.0 45 167287.0 46 162209.5 47 156424.0 48 149266.5 49 139721.0 50 128782.5 51 116385.0 52 102396.0 53 87157.0 54 72099.5 55 58939.5 56 47977.0 57 39038.5 58 31783.0 59 25560.0 60 20197.0 61 15874.5 62 12174.5 63 9232.0 64 7026.5 65 5284.5 66 3993.0 67 3030.5 68 2295.5 69 1748.5 70 1348.5 71 1029.5 72 791.0 73 588.0 74 442.0 75 350.0 76 281.5 77 212.5 78 155.5 79 126.0 80 86.0 81 66.5 82 55.5 83 40.5 84 33.0 85 31.0 86 23.0 87 16.0 88 16.5 89 10.0 90 5.5 91 5.5 92 2.5 93 2.0 94 2.0 95 1.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16201915594980407 2 0.0 3 1.1318138732085509E-4 4 0.021504463590962468 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.2732906073596197E-4 12-13 2.8295346830213772E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 2.8295346830213772E-5 28-29 9.90337139057482E-5 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0010893708529632303 38-39 5.800546100193823E-4 40-41 2.8295346830213775E-4 42-43 5.093162429438479E-4 44-45 3.1124881513235145E-4 46-47 0.0032681125588896907 48-49 0.0037915764752486456 50-51 0.004244302024532066 52-53 0.004003791576475248 54-55 0.005970318181175106 56-57 0.009351612127385653 58-59 0.017005503444958478 60-61 0.004682879900400379 62-63 0.002829534683021377 64-65 0.010299506246197814 66-67 0.023103150686869543 68-69 0.02553655051426793 70-71 0.025678027248418996 72-73 0.022565539097095483 74-75 0.024744280803021945 76-77 0.024800871496682374 78-79 0.01559073610344779 80-81 0.00340958929304076 82-83 0.0011459615466236578 84-85 0.005376115897740617 86-87 0.014473069903654344 88-89 0.016566925569090164 90-91 0.023386104155171682 92-93 0.02385297737787021 94-95 0.025041381944739188 96-97 0.026668364387476478 98-99 0.03268112558889691 100-101 0.023470990195662324 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3534150.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 93.02534757777813 #Duplication Level Percentage of deduplicated Percentage of total 1 97.64965045258053 90.83892674199842 2 1.7401338996376696 3.2375312169133736 3 0.24981738451720473 0.6971804707705325 4 0.13492579776739663 0.5020607693808429 5 0.04744336767612987 0.22067178841661522 6 0.03738284623604289 0.2086531358732715 7 0.03962756977811879 0.25804579165905095 8 0.009918334372736414 0.07381252019331432 9 0.01176863417164863 0.09853031558999976 >10 0.07612352831164984 1.2379611232930567 >50 0.002192688287477131 0.14611764164498636 >100 9.185525418843795E-4 0.15867335246842318 >500 3.530552625037636E-5 0.027818095966764135 >1k 3.129870654761901E-5 0.04381917448267344 >5k 0.0 0.0 >10k+ 3.0339888723547E-5 2.2501978613486937 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT 79727 2.2559031167324535 TruSeq Adapter, Index 13 (97% over 38bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.004753618267475913 0.0 0.0 2 0.006168385608986602 0.0 0.0 3 0.008828148211026697 0.0 0.0 4 0.011318138732085509 0.0 0.0 5 0.014374036189748595 0.0 0.0 6 0.024192521539832774 0.0 2.8295346830213772E-5 7 0.03528429749727657 0.0 5.6590693660427545E-5 8 0.043829492240001135 0.0 5.6590693660427545E-5 9 0.049460266259213674 0.0 1.1318138732085509E-4 10-11 0.05936363764978849 0.0 1.1318138732085509E-4 12-13 0.07161552282727104 0.0 1.1318138732085509E-4 14-15 0.08266485576446952 0.0 1.1318138732085509E-4 16-17 0.09671349546567068 0.0 1.2732906073596197E-4 18-19 0.11414342911308235 0.0 1.4147673415106885E-4 20-21 0.1358318124584412 0.0 2.122151012266033E-4 22-23 0.1584256469023669 0.0 2.4051044805681706E-4 24-25 0.18002914420723512 0.0 2.9710114171724463E-4 26-27 0.2019014473069904 0.0 3.112488151323515E-4 28-29 0.2254290281963131 0.0 3.112488151323515E-4 30-31 0.25751595150177553 0.0 3.67839508792779E-4 32-33 0.29219189904220255 0.0 3.819871822078859E-4 34-35 0.3308857858325198 0.0 4.2443020245320654E-4 36-37 0.3772477116138251 0.0 4.5272554928342036E-4 38-39 0.434022325028649 0.0 4.95168569528741E-4 40-41 0.4960315776070625 0.0 5.234639163589547E-4 42-43 0.5640111483666511 0.0 5.800546100193822E-4 44-45 0.6357540002546582 0.0 5.942022834344891E-4 46-47 0.7119250739215937 0.0 5.942022834344891E-4 48-49 0.7928922088762503 0.0 6.3664530367981E-4 50-51 0.8795750038906102 0.0 6.507929770949168E-4 52-53 0.9690873335879915 0.0 6.507929770949168E-4 54-55 1.0663950313370967 0.0 6.649406505100236E-4 56-57 1.1679045880904884 0.0 7.49826691000665E-4 58-59 1.272936915524242 0.0 8.064173846610925E-4 60-61 1.3818457054737348 0.0 8.205650580761993E-4 62-63 1.4898773396714908 0.0 8.77155751736627E-4 64-65 1.603992473437743 0.0 8.77155751736627E-4 66-67 1.7265537682328143 0.0 9.054510985668407E-4 68-69 1.8490584723342245 0.0 9.054510985668407E-4 70-71 1.977094916740942 0.0 9.337464453970545E-4 72-73 2.1116817339388536 0.0 9.761894656423752E-4 74-75 2.2513475658927886 0.0 9.90337139057482E-4 76-77 2.395936788195181 1.4147673415106886E-5 0.0010186324858876957 78-79 2.545067413663823 2.8295346830213772E-5 0.0010752231795481233 80-81 2.7012152851463576 2.8295346830213772E-5 0.0011318138732085508 82-83 2.863177850402501 2.8295346830213772E-5 0.0011601092200387646 84-85 3.030785337351273 2.8295346830213772E-5 0.0012025522402840851 86-87 3.2034859867294823 2.8295346830213772E-5 0.0012166999136991922 88-89 3.3886931794066464 2.8295346830213772E-5 0.001244995260529406 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGACC 30 1.2906997E-4 63.44105 1 CGGAAGA 23355 0.0 39.475433 4 GAGCACA 23110 0.0 39.332317 9 AGAGCAC 23535 0.0 38.944904 8 TCGGAAG 23595 0.0 38.731678 3 ATCGGAA 24100 0.0 37.920082 2 GCCGTCT 12050 0.0 37.566383 50-51 CGTCTTC 12095 0.0 37.48685 52-53 GAAGAGC 24595 0.0 37.44023 6 GATCGGA 24560 0.0 37.409817 1 CCGTCTT 12145 0.0 37.136963 52-53 TGCCGTC 12210 0.0 36.937954 50-51 ATGCCGT 12305 0.0 36.747723 48-49 TATGCCG 12455 0.0 36.381428 48-49 AAGAGCA 25635 0.0 35.698986 7 CTGCTTG 12945 0.0 35.35588 58-59 CGTATGC 12885 0.0 35.239532 46-47 TGCTTGA 12720 0.0 35.06808 60-61 GCTTGAA 12675 0.0 34.874012 60-61 TTGTGTT 21275 0.0 34.59811 1 >>END_MODULE