FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l07n01_34211-2.34100000035863.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l07n01_34211-2.34100000035863.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3534150
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT797272.2559031167324535TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGACC301.2906997E-463.441051
CGGAAGA233550.039.4754334
GAGCACA231100.039.3323179
AGAGCAC235350.038.9449048
TCGGAAG235950.038.7316783
ATCGGAA241000.037.9200822
GCCGTCT120500.037.56638350-51
CGTCTTC120950.037.4868552-53
GAAGAGC245950.037.440236
GATCGGA245600.037.4098171
CCGTCTT121450.037.13696352-53
TGCCGTC122100.036.93795450-51
ATGCCGT123050.036.74772348-49
TATGCCG124550.036.38142848-49
AAGAGCA256350.035.6989867
CTGCTTG129450.035.3558858-59
CGTATGC128850.035.23953246-47
TGCTTGA127200.035.0680860-61
GCTTGAA126750.034.87401260-61
TTGTGTT212750.034.598111
Produced by FastQC (version 0.11.2)